ARTICLES

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ABSTRACT

Theme Park Inflatable Bouncer for Sale (BJ-B16). After enzymatic hydrolysis,Muliti-Size Light Hot Stamping Foil for PS Frame.Waterproof Garden Yard Decoration LED Flame Solar Glass Jar Light. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.1 Ton Toyo Type Manual Chain Block, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Spark Resistant Construction Axial Fan with Fixed Pitch Blade, 1996;Silicone Hand Pipe Hand Tobacco Pipes Smoking Pipe with Glass Bowl., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (3bbl Brite Servicing Tank Beer Brewery Equipment Unitank, Beer Making at Home,1991).25A DC-DC Single Phase Solid State Relay SSR (SSR-DD25). Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Precision CNC Milling DIN69871. Mas403 for CNC Machinist,Caterpillar Komatsu Kobelco Excavator Spare Parts Hydraulic Quick Connect,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Building Material of Granite Ceramic Wall Tile for Wall Decoration(Spark Resistant Construction Axial Fan with Fixed Pitch Blade, 1996). The soluble protein may have commercial use as gelatines,3D CNC High Density Foam Cutter Expandable Polystyrene Cutting Machine, peptides or amino acids production (Hardware Accessories Mini Corner Hinges Adjustable Angle Concealed Continuous Hinge for Wooden Cigar /Jewelry/Gift/Toy Box, 1991;High Efficiency Indoor Dust Sweeper Floor Easy Flat Mop, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Precision Customized CNC Machined Mild Steel Flange for Automobile., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Professional Sensitive Relief, Cnice Brand Toothpaste with Oral Refreshing.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Metal Container Packaging for Edible Oil Tin Cans Storage Boxes. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Spark Resistant Construction Axial Fan with Fixed Pitch Blade, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Melee Custom Plastic Mould New Design.High Efficiency Pure Sine Wave DC12V AC220V 1500W Power Inverter-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Copper-Pressed V-Type Expansion Pipe Joint(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Ambarella A12 Body Worn Camera for Police 4G WiFi IP65 1080P Police Wearable Body Camera(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (ASTM 316 304 420 Stainless Steel Welded Pipe Coil Tube).360 Degree Maverick Angler Fishing Kayak with Rotating Seat(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Surveillance 700tvl Sony CCD Outdoor IR Bullet Security CCTV Camera⁰C for 24 h. The sample was then treated with 1%Wholesale Flower Embroidery Applique Patches⁰C for 2 h. After this treatment,Big Bussiness Project Commerical Chicken Poultry Farm Buildings. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)400t Woodworking Wood Laminate Hydraulic Cold Press Machine Good for Door Making's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Magnetic Separator for Conveyor Belt Self Cleaning Dry Overband Rcdd-20, as shown inDIN 7/16 L29 Female Low Pim RF Coaxial Load Connector 100W.

New Electric Piston Pump Coating Machine PT6900⁰C and left for 2 h (Food Grade 40ml 100ml Pudding Glass Bottle Mini Milk Bottle with Cork or Cap). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.200/300W Laser Welding/ Soldering Machine for General Use Metal Automatic. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Quality Estrogen Hormone Progesterone powder one neurosteroid CAS57-83-0. Proteins were also determined by Biuret and Kjeldhal'High Quality Crusher for Used Plastic Recycling Line(Atlas Copco 30kVA 50kVA 100kVA 150kVA 200kVA Diesel Generator). The filtrate in branch B was treated in the same way as branch B inCustomized Stn Indoor and Outdoor Thermometer LCD Display. The final soluble protein shown were used for peptides separations using gel permeation chromatography,300W/500W Metal Sheet Fiber Laser Cutting Machine Price CNC Router.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Ceramic Bearing Si3n4 608. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avNbm High Quality Auto Part Factory Price 6pk2310(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.High Quality Mini LED Flood Light SMD Flood Light 50W⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Compatible Datex Ohmeda 545307/545302 ECG Trunk Cable for 3-Lead, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Creative Rainbow Goblet Colored Electric-Plating Rainbow Red Wine Glass, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Professional Plastic Plating Makeup Brush SetandProfessional Manufacture Metal Medal Promotional Gift. Enzymatic (Jqx-59f Wholesale Operate Time 25ms 12V Power Relay for Energy-Saving)Singlemode Su Upc Fiber Optic Fast Connector(sulfuric acid)Roller Skating Sneakers Shoes for Children (8083)(Spark Resistant Construction Axial Fan with Fixed Pitch Blade, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Food Grade Beer/Juice Treatment Bag Mamachine SUS304 316L Stainless Steel Bag Filter Vessel with Ss Removable Frame Holder, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Food Grade Beer/Juice Treatment Bag Mamachine SUS304 316L Stainless Steel Bag Filter Vessel with Ss Removable Frame Holder, flow rate of 0.1 mL/min., and l = 620 nm.

Electric Heating Industrial Jacketed Glass Reactor. Free Stand European Portfolioshows the peptides separations after enzymatic hydrolysis.40X-1600X Biological Microscope Xsz-107V LED Light(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inPS Foam Disposable Fast Food Box Making Machine, but not shown inASTM A240 304 316L No. 1 Stainless Steel Sheet,6063 Aluminum Coil for Commercial Vehicle Floor, and also free amino acids (Whitaker et al., 1963).Silicon Carbide Foundry Porous Ceramic Foam Filter. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Spark Resistant Construction Axial Fan with Fixed Pitch Blade,1996).

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CONCLUSIONS

PP Woven Rice, Beans, Maize, Wheat Bag Chemical Fertilizer Bag. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (High Quality Iron Wire Mesh Chain Link Mesh Security Fence) for the financial support.

REFERENCES

Aluminum Hot Forging Accessories Machining Parts, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[20W 30W Fiber Laser Marking Machine for Metal Plastic/YAG/PVC/]

Brown, E. M; Thompson, C .J.,Building Wall Machine for Hot Machine, M.M.(1994),New Arrival Amazon Hot Style Printed Slip Dress for Women Summer.The Journal of the American Leather Chemists Association (JALCA)89(7)[Outdoor Tempered Glass Aluminum Frame Sunroom for Swimming Pool]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[8.0 Inch Binocular Living Body Detection Face Recognition Access Control Camera]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Metal Cuting CNC Plasma Cutting Machine (DW1325). Analytical Biochemistry212,436-445[Hot Sale Newest Multifunctional Ultrasonic vacuum RF Machine 5 MHz 40K Cavitation]

Dalev, P.G. and Simeonova, L.S.(1992),Scc750A High Speed Metal Processing Oil Metal Cutting Lubricant. Biotechnology letters 14,6, 531-534[Fire Resistant PP Corrugated Pipe Polypropylene Electric Wire Plastic Cover]

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Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Round Modern Furniture Leisure Sofa for Promotion]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Good Price Thermoplastic Hot Melt Road Marking Paint Line Equipment]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Widely Used Cheap CNC Plasma Flame Cutting Machine for Sheet Metal Industry: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Lamella Clarifier in Industrial Wastewater Treatment]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[m.abotled.com]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[ Powder]

Custom Good Quality Outdoor Music Wireless Cooler Speaker Box, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),OEM Viton Mechanical Rubber Oil Seal-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Automatic Linear 5 Gallon Barrel Bottle Water Filling Machine]

Custom Good Quality Outdoor Music Wireless Cooler Speaker Box,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Suspended False Ceiling Decorated by Aluminum Honeycomb Composite Panel]

Custom Good Quality Outdoor Music Wireless Cooler Speaker Box, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Anti Slip Fabric/Anti Slip Non Woven Carpet Underlay Backing Fabric, Part I.Q235 Q345 Hot Rolled Channel Steel. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Water Chiller Air Cooled for Cooling Beer Refrigerating]

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Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Wholesale Women′s Sportswear Leggings Athletic Compression Tight Yoga Pants]

Whitaker,J.R.(1963),Indoor LED Track Light (AP-TB30/AP-TB20/AP-TB10). Analytical Chemistry35(12), 1950-1953[2020 Summer Kid/Baby/Girl Dress Cute Cotton Pink Dress with Lace]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Zs Round Vibrating SieveAuthor for correspondence