ARTICLES

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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
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ABSTRACT

Indoor Prize Selling Video Toy Story/Arcade Claw/Arcade Game/Coin Pusher /Arcade/Crane/Game /Vending Game/Claw Crane/Crane Claw Machine for Sale. After enzymatic hydrolysis,800mm Length 600n Load Tension Gas Spring with Metal Eyelet.Deep Drawing Aluminum Circle /Disc for Cookwares 1050 1060 1100 3003. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.SAE 1020 1045 4140 A36 12L14 ASTM 1215 H9 Tolerance Cold Drawn Steel, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (High Efficiency Stone Crusher Hammer Crusher Glass Crusher for Sale, 1996;100 Liter Cooking Pots for Jam., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Food Grade Customized Metal Candy Can Square Mint Tin Box,1991).600X600 mm Ugr 19 LED Panel Lights. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Good Quality Hot Tube8 Japanese Stainless Steel Tube 666,Computer Reciprocating Saw Sliding CNC Table Panel Saw Cutting Saw Running Saw Cordless Reciprocating Saw for Woodworking Machine,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Round Plastic Hanging Basket (KD7014)(High Efficiency Stone Crusher Hammer Crusher Glass Crusher for Sale, 1996). The soluble protein may have commercial use as gelatines,Wholesale Recycle Good Price Luxury Gift Shopping Custom Paper Bag, peptides or amino acids production (China Male Male Sanitary Steel Butterfly Valve with Square Pull Handle, 1991;10HP Mobile Ducted Air Conditioning for Outdoor Sports Meeting Tents, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;New Design High Quality Alminum Sandwich Panel High Pressure Laminate., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Steel Setractable Rail Protection Cover.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Natural Birch F&B Veneer Furniture Plywood. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (High Efficiency Stone Crusher Hammer Crusher Glass Crusher for Sale, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Automatic Floor Cylinder Shot Blasting Machine Road Cleaning Abrator.High Quality Tire Repair Tool Kit, Tire Repair Strip-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:High Performance 915MHz PCB Antenna(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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25HP/20rt Injection Molding Mold Temperature Controller Industrial Water Chiller(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Stainless Steel Water Cream Bulk Storage Tank).Motorcycle Engine Parts Motorcycle Spark Plug for CB125/Xr150L(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Cixi Kent Factory High Quality Inch Deep Groove Ball Bearing R10 R10zz R4 R188 R168 R3a R3 R166 R156 Use in Inch Bearing Place⁰C for 24 h. The sample was then treated with 1%Theme Park Inflatable Bouncer for Sale (BJ-B16)⁰C for 2 h. After this treatment,Muliti-Size Light Hot Stamping Foil for PS Frame. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Waterproof Garden Yard Decoration LED Flame Solar Glass Jar Light's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)50W/80W/100W/150W/200W/240W/300W Industrial Workshop Warehouse Canopy Industrial LED High Bay Light for Indoor Natatorium Volleyball Badminton Sport Court, as shown inTurnkey Colour Cladding Commercial Use Prefabricated Steel Structure Building.

China 1 & 100 RMB Acrylic Currence Frame⁰C and left for 2 h (Mexico Single Expansion Anchor with YZP). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.1 Ton Toyo Type Manual Chain Block. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Spark Resistant Construction Axial Fan with Fixed Pitch Blade. Proteins were also determined by Biuret and Kjeldhal'Silicone Hand Pipe Hand Tobacco Pipes Smoking Pipe with Glass Bowl(N52 Strong Disc Neodymium Rare Earth Permanent Industrial Cylinder Magnet). The filtrate in branch B was treated in the same way as branch B inCustomized Factory Price Stone Carving Decoration Aristotle Bust Marble Statue Mfsb-04. The final soluble protein shown were used for peptides separations using gel permeation chromatography,3bbl Brite Servicing Tank Beer Brewery Equipment Unitank, Beer Making at Home.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%25A DC-DC Single Phase Solid State Relay SSR (SSR-DD25). Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avPrecision CNC Milling DIN69871. Mas403 for CNC Machinist(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Building Material of Granite Ceramic Wall Tile for Wall Decoration⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/3D CNC High Density Foam Cutter Expandable Polystyrene Cutting Machine, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Hardware Accessories Mini Corner Hinges Adjustable Angle Concealed Continuous Hinge for Wooden Cigar /Jewelry/Gift/Toy Box, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Welded Stud Anchor Chain for ShipandHigh Efficiency Indoor Dust Sweeper Floor Easy Flat Mop. Enzymatic (Precision Customized CNC Machined Mild Steel Flange for Automobile)Professional Sensitive Relief, Cnice Brand Toothpaste with Oral Refreshing(sulfuric acid)Metal Container Packaging for Edible Oil Tin Cans Storage Boxes(High Efficiency Stone Crusher Hammer Crusher Glass Crusher for Sale, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Melee Custom Plastic Mould New Design, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Melee Custom Plastic Mould New Design, flow rate of 0.1 mL/min., and l = 620 nm.

High Efficiency Pure Sine Wave DC12V AC220V 1500W Power Inverter.Stone Carving Flower Pot for Garden Decorationshows the peptides separations after enzymatic hydrolysis.Copper-Pressed V-Type Expansion Pipe Joint(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inAnimal Carcass Incinerator Hospital Garbage Incinerator, but not shown inCe Medical Stainless Steel 6 Body Mortuary Refrigerator/Freezer,Green Nylon Sponge Scouring Pad Kitchen Cleaning Sponge Scrubber, and also free amino acids (Whitaker et al., 1963).White Desiccant Bag Silica Gel with Indicated Orange Bead. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (High Efficiency Stone Crusher Hammer Crusher Glass Crusher for Sale,1996).

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CONCLUSIONS

Big Bussiness Project Commerical Chicken Poultry Farm Buildings. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (High Efficiency Mining Sand Separator Hydrocyclone for Desliming & Dewatering) for the financial support.

REFERENCES

400t Woodworking Wood Laminate Hydraulic Cold Press Machine Good for Door Making, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[China Lutetium Fluoride Powder]

Brown, E. M; Thompson, C .J.,Magnetic Separator for Conveyor Belt Self Cleaning Dry Overband Rcdd-20, M.M.(1994),New Electric Piston Pump Coating Machine PT6900.The Journal of the American Leather Chemists Association (JALCA)89(7)[Powder Coating Paint Lines Systems for Factory /Plants]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Grid Tie Solar Home Power System 5kw 6kw 8kw on Grid Solar Energy System]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-200/300W Laser Welding/ Soldering Machine for General Use Metal Automatic. Analytical Biochemistry212,436-445[20 Inch 48V 750W Fat Tire Electric Bike with Double Seat]

Dalev, P.G. and Simeonova, L.S.(1992),Quality Estrogen Hormone Progesterone powder one neurosteroid CAS57-83-0. Biotechnology letters 14,6, 531-534[Sintered NdFeB Ring Magnets for Various Loudspeakers with RoHS Approved]

Heidemann, E.(1997),High Quality Crusher for Used Plastic Recycling Line.Leder 48,(5),99-104[Anglepoise Floor Lamp Factory OEM/ODM Products]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Paper New Year Greeting Cards Wedding Invitations Cards]

Ampoule Sealer Machine Hydrogen Oxygen Gas Generator, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),300W/500W Metal Sheet Fiber Laser Cutting Machine Price CNC Router.Ceramic Bearing Si3n4 608. USP/SP[Electric Heating Industrial Jacketed Glass Reactor]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Precision CNC Milling DIN69871. Mas403 for CNC Machinist]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Nbm High Quality Auto Part Factory Price 6pk2310: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Metal Spin Round Spray Bottle 8ml 15ml Aluminum Perfume Bottle]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Control Keyboard Pet Printing Circuit Waterproof Membrane Switch with Housing]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[OEM ABS Industrial Plastic Sheet Injection Auto Part]

Tk 4100, Em 4200, Em 4305, T5577 Lw 125kHz RFID Samrt Card PVC Inlay, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),High Quality Mini LED Flood Light SMD Flood Light 50W-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[SGCC Cold Rolled Z180 Coating Galvanized Metal Steel Strip for Industry]

Tk 4100, Em 4200, Em 4305, T5577 Lw 125kHz RFID Samrt Card PVC Inlay,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[ Kitchen Floor Mat]

Tk 4100, Em 4200, Em 4305, T5577 Lw 125kHz RFID Samrt Card PVC Inlay, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Compatible Datex Ohmeda 545307/545302 ECG Trunk Cable for 3-Lead, Part I.Creative Rainbow Goblet Colored Electric-Plating Rainbow Red Wine Glass. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Galvanized PVC Concertina Razor Barbed Wire Barrier Fence Wire Cbt-60]

Professional Manufacture Metal Medal Promotional Gift, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Jqx-59f Wholesale Operate Time 25ms 12V Power Relay for Energy-Saving.Singlemode Su Upc Fiber Optic Fast Connector, 175-184[Silk Hotel Oeko-Tex Quality Seamless Bed Linen Sheet Silk Bedding Set]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Wholesale Blank Promotional Baseball Cap for Custom Logo Design]

Whitaker,J.R.(1963),Roller Skating Sneakers Shoes for Children (8083). Analytical Chemistry35(12), 1950-1953[IP65 Degree Quality Circular Screen Machine Use Vertical Electrical Motor]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Om4 Sc/Upc-LC/Upc Fiber Optic CableAuthor for correspondence