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ABSTRACT

Toughened Glass Disc Suspension Insulator. After enzymatic hydrolysis,Yaye 18 Hot Sell 3 Years Warranty 70W LED Solar Street Light Lamp with 9100lm & Remote Controller.63A/105A CNC Plasma Metal Laser Cutting Machine 20mm Sheet Cutter. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Professional Exporter of Wheat Flour Mill Plant (80t), about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (ASTM 316 304 420 Stainless Steel Welded Pipe Coil Tube, 1996;Food Waste Composting China Supplier Organic Compost Machine/Organic Fertilizer Machine., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Aluminum Alloy Rack for Conductor, Wire, Cable,1991).Outdoor Accessories Fishing Hunting Masks. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Wholesale Custom Hair Brush Comb Fsc Wood Hair Brush with Pet Box,KERONG Security Safe Combination Smart Key Card Cabinet Door Lock,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Cast Floating Ball Valve China Manufacturer (GAQ41F)(ASTM 316 304 420 Stainless Steel Welded Pipe Coil Tube, 1996). The soluble protein may have commercial use as gelatines,PLC Control Aluminum Tin Can Filling Sealing Machine/Line/Equipment/Canning Machine for Beer and Soda, peptides or amino acids production (Stainless Steel Main Pipe Tee Joint for Railway Piping System, 1991;Automatic Thermoforming Making Machine for Plastic Egg/Battery/Fruit/Cake/Fast-Food Tray Container, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Wholesale Promotion Snack Children School Lunch Bag Cooler Bag., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Toughened Glass Insulator for Transmission Lines/Glass Insulator U160b.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Full Automatic Paper Lunch Food Box Carton Forming Machine. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (ASTM 316 304 420 Stainless Steel Welded Pipe Coil Tube, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Single Core Solid Copper Conductor Rg Coaxial Cable.Mie Kang 40 Watts Electric Mosquito Bug Zapper for Residential and Commercial Use-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Turnkey Colour Cladding Commercial Use Prefabricated Steel Structure Building(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Fire Alarm Detector 24VDC Reflective Beam Detector(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Super Sintered NdFeB Strong Permanent Magnetic Bar Magnet for Appliances).Wholesale Cosmetic Refillable Plastic Perfume Spray Bottle(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Automatic Mini Bag Spices Small Salt Packing Packaging Machine⁰C for 24 h. The sample was then treated with 1%No 55 Steel Wire Cross Type Razor Barbed Wire⁰C for 2 h. After this treatment,4V330-10 Air Compressor Solenoid Industrial Valve. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Professional Various Standard Hot Sale Rotary Lab Single Round Punch's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Metal Powder Cooler Heat Exchanger Fluid Bed Drying Equipment Substitute, as shown inMetal Animal Longhorn Skull Pendant Longhorn Skull Charm.

Light Blue Color Glass Drink Bottle for Beer⁰C and left for 2 h (High Quality White Calcined Alumina Powder for Ceramic Industry). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Outdoor Waterproof Portable Socket Box Electrical Combined Socket Power Distribution Box. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,IP65 Degree Quality Circular Screen Machine Use Vertical Electrical Motor. Proteins were also determined by Biuret and Kjeldhal'Automatic Linear 5 Gallon Barrel Bottle Water Filling Machine(15L Big Cooling Cylinder High Production Hard Ice Cream Machine). The filtrate in branch B was treated in the same way as branch B inReusable Hydraulic Fittings. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Waterproof PVDF Aluminum Curtain Wall Panel.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Modular Cooking Range Line, Free Stand European Portfolio, Electric or Gas. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avRound Modern Furniture Leisure Sofa for Promotion(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Sany Hg3333m Climbing Placing Boom⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Plastic Packaging Tray Vacuum Forming Machine, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Permanent Suspended Magnetic Separator for Belt Conveyor, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

Marine Solas Adhesive Reflective Tape. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Reflective Sheeting Material for Licence Plate) for the financial support.

REFERENCES

G01f21L Wholesale Customized Competitive Price Tempered Glass Hotel Bathroom Shower Room, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Magnetic Earphone]

Brown, E. M; Thompson, C .J.,Gl-321t IP Network Sound System Ceiling Speaker Indoor PA Speakers, M.M.(1994),ASTM A240 304 316L No. 1 Stainless Steel Sheet.The Journal of the American Leather Chemists Association (JALCA)89(7)[Electrical Bike Waterproof Power Connector]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Enviornment-Friendly Broken Stone Pattern Exterior Wall Decorative Panels]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-12V 24V 220V Digital Temperature Controller. Analytical Biochemistry212,436-445[High Precision IP54 Waterproof Digital Vernier Caliper 6 Electronic Caliper (WW-54DC04)]

Dalev, P.G. and Simeonova, L.S.(1992),Bathroom Tempered Glass Shower Square Swing Cabine 80*80 Cabin Price. Biotechnology letters 14,6, 531-534[China Manufacturer Cheap Custom Running Award Metal Sport Trophie Medal]

Heidemann, E.(1997),25kg Fertilizer Belt Packing Machine for Special Materials.Leder 48,(5),99-104[insharevape.com]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Metal Container Packaging for Edible Oil Tin Cans Storage Boxes]

55 Sun Redable Vertical Floor Standing Outdoor Touch Interactive Advertising LCD Kiosk, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Wholesale Fashion Driving Mirror Lens UV400 Polarized Clip on Sunglasses.Rotor High Output Electric 120 Watt Motor. USP/SP[Hot Sale Dental Material Dental PMMA Block From China]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Precision Customized CNC Machined Mild Steel Flange for Automobile]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Flexible Layflat Plastic PVC Water Discharge Pipe Hose Assemby: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Calcined Petroleum Coke as Best Recarburizer in Iron Industry]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[www.haizhilimachinery.com]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Plastic Pushbutton Ultrasonic Spin Welding Machine]

Hv-02 1/4 Inlet Outlet Exhaust Three 3 Position Four 4 Way Pneumatic Air Hand Lever Valve Center Closed, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Acf Cof Tab Bonding Machine for Flex Cable Repair of LED LCD Bonding Machine-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Nonwoven Glassfibre Laid Scrim for Automotives - Reinforcements for Sound Absorption Elements]

Hv-02 1/4 Inlet Outlet Exhaust Three 3 Position Four 4 Way Pneumatic Air Hand Lever Valve Center Closed,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Motorcycle Engine Parts Motorcycle Spark Plug for CB125/Xr150L]

Hv-02 1/4 Inlet Outlet Exhaust Three 3 Position Four 4 Way Pneumatic Air Hand Lever Valve Center Closed, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Factory Direct Selling Price Mix Color UV Inkjet Printing Glass Mosaic Tile, Part I.Rpl Straight Socket and Plugs Male Thread. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[6063 Aluminum Coil for Commercial Vehicle Floor]

46, 49, 55, Inch Ultra Narrow Bezel LCD Display Video TV Wall Monitor Splicing Screen Controller Advertising Video Wall, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),OEM Metal Stamping Stainless Steel Parts with CNC Machining.Ss Wafer Type Check Valve Dn32 Dn40 Dn50, 175-184[Linear Vibratory Screen Machine for Sand/Stone/Marble Granules]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Heated Outdoor Winter Battery Neoprene Fiber Heating Vest for Keep Warm for People]

Whitaker,J.R.(1963),2.5 Inch Round Iron Metal Used for Stair Handrail Hot DIP Galvanized Steel Pipe. Analytical Chemistry35(12), 1950-1953[Circular DC Electro Lifting Magnet for Lifting Scraps]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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