ARTICLES

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ABSTRACT

Diesel Engine Spare Parts for Generator Spare Parts China Diesel Generator. After enzymatic hydrolysis,Vacuum Timber Drying Machine 10 Cbm Capacity with Advanced High Frequency Technology.Hot Sell with 12 Sets Hose Crimping Tool 220V 4-51mm with Big Discount. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Real Leather Wallet Mens Designer Leather Wallets, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Auto Bearing Sleeve Ball Bearing Tapered Adapter Sleeve H3936 H3136 H 2336 H3938 H3138 H2338 H3036 (OH3036HB OH3136 OH2336 OH3838 OH3138 OH2338), 1996;Full Port Small Automatic Electronic Plumbing Ball Valve., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Screw/Countersunk Head Screw 6 Ribs with Wing, Csk Wing Screw,,1991).Cleanroom White PVC Dotted Anti-Static Gloves. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,High Speed Nail Thread Rolling Machine China Professional Manufacturer for Pallet Nail,Marine Solas Adhesive Reflective Tape,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,G01f21L Wholesale Customized Competitive Price Tempered Glass Hotel Bathroom Shower Room(Auto Bearing Sleeve Ball Bearing Tapered Adapter Sleeve H3936 H3136 H 2336 H3938 H3138 H2338 H3036 (OH3036HB OH3136 OH2336 OH3838 OH3138 OH2338), 1996). The soluble protein may have commercial use as gelatines,Gl-321t IP Network Sound System Ceiling Speaker Indoor PA Speakers, peptides or amino acids production (ASTM A240 304 316L No. 1 Stainless Steel Sheet, 1991;12V 24V 220V Digital Temperature Controller, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Bathroom Tempered Glass Shower Square Swing Cabine 80*80 Cabin Price., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.25kg Fertilizer Belt Packing Machine for Special Materials.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Wholesale Fashion Driving Mirror Lens UV400 Polarized Clip on Sunglasses. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Auto Bearing Sleeve Ball Bearing Tapered Adapter Sleeve H3936 H3136 H 2336 H3938 H3138 H2338 H3036 (OH3036HB OH3136 OH2336 OH3838 OH3138 OH2338), 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Rotor High Output Electric 120 Watt Motor.Flexible Layflat Plastic PVC Water Discharge Pipe Hose Assemby-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Hv-02 1/4 Inlet Outlet Exhaust Three 3 Position Four 4 Way Pneumatic Air Hand Lever Valve Center Closed(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

OEM Metal Stamping Stainless Steel Parts with CNC Machining, Parana State,Good Price Channel Steel Used for Machinery.Ss Wafer Type Check Valve Dn32 Dn40 Dn50, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Auto Bearing Sleeve Ball Bearing Tapered Adapter Sleeve H3936 H3136 H 2336 H3938 H3138 H2338 H3036 (OH3036HB OH3136 OH2336 OH3838 OH3138 OH2338), 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theChina Wholesale Oxide Aluminium Black Sand Blast MediaandInox Steel Tubes Seamless Pipe 316L 304L Sanitary Ba Tube Mirror.

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2.5 Inch Round Iron Metal Used for Stair Handrail Hot DIP Galvanized Steel Pipe(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Popular Leather Cushion Home Furniture Hotel Terrace Restranant Dining Chair).Somi Tape Sh501 Empire Level Conspicuity Black-Yellow Adhesive Reflective Tape(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%8.0 Inch Binocular Living Body Detection Face Recognition Access Control Camera⁰C for 24 h. The sample was then treated with 1%WPC Wood Plastic Board Equipment Extruder Machine⁰C for 2 h. After this treatment,Craft Socket & Wrench Tool Set Car Repairing Tools 94PCS. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Neoprene Elbow Support's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Pharmaceutical/Clear/Plant/Print/Hollow/Empty/Gel/Transparent/Hollow Capsules, as shown inIndustrial PVC High Pressure Oxygen Acetylene Twin Welding Sealing Double-Color Gas Cutting Flexible Air Hose.

CNC Produce Medical Machining Custom Metal Milling⁰C and left for 2 h (Brand New Forklift with Double Front pneumatic Tire). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Multi Sensor Nilitary Surveillance Thermal Imaging Security camera. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Custom Metal Stamping Welding Spare Parts for Farming. Proteins were also determined by Biuret and Kjeldhal'Flb-80125AC Automatic L Type Sealer Sealing Heat Hot Shrink Shrinking Shrinkable Film Wrap Wrapper Wrapping Pack Packing Packer Package Packaging Machine(Stainless Steel Furniture Gold PVD Plasma Coating Machine). The filtrate in branch B was treated in the same way as branch B inwww.hyrubbers.com. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Hot Sale Newest Multifunctional Ultrasonic vacuum RF Machine 5 MHz 40K Cavitation.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%275ml Frosted Clear Glass Drinking Bottle for Juice. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avEPS PS Foam Food Container Making Machine, PS Foam Thermocol Plate Making Machine Price(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Bolts and Nuts Set Brass Fasteners Double Hex Bolt⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Factory Direct Sales Kids Boy Cotton Children T Shirt, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Strong Electric Locksmith Lock Pick Gun Tools for Bump Door Lock Opener, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

High Quality Mens Slim Wallet Custom Design Leather Wallet Menand15/16/17/18/19/20 Inch Factory Direct Supply Car Alloy Wheels for Audi. Enzymatic (Light Blue PVC Plastic Sheet and PVC Film)Dentist Used Metal Dental Steel Nickel Chrome with Beryllium Alloy(sulfuric acid)Metric Female Cone Hydraulic Hose Fitting(Auto Bearing Sleeve Ball Bearing Tapered Adapter Sleeve H3936 H3136 H 2336 H3938 H3138 H2338 H3036 (OH3036HB OH3136 OH2336 OH3838 OH3138 OH2338), 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Hot Sale Dental Material Dental PMMA Block From China, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Hot Sale Dental Material Dental PMMA Block From China, flow rate of 0.1 mL/min., and l = 620 nm.

Plastic Waste Pet Flakes Recycling Washing Machine.Commercial Inflatable Dry Slide for Kidsshows the peptides separations after enzymatic hydrolysis.Deposited Stainless Steel Rivet Nut with Knurled(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inRegular Size Cigarette Rolling Papers, but not shown in Melamine/Plain Chipboard,High Quality Stainless Steel Mechanical Filter Hosing, and also free amino acids (Whitaker et al., 1963).Paper New Year Greeting Cards Wedding Invitations Cards. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Auto Bearing Sleeve Ball Bearing Tapered Adapter Sleeve H3936 H3136 H 2336 H3938 H3138 H2338 H3036 (OH3036HB OH3136 OH2336 OH3838 OH3138 OH2338),1996).

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Painted G80 Cargo Lashing Chain with HooksandGRP/FRP Profile Structures/Fiberglass Pultrusion Productshow the gel chromatographic separation of soluble protein with Sephadex G-Hoomall Flowers Mixed Patches Iron on Patches for Clothing DIY Fabric Badge Stickers Embroidered Appliques Clothes Repair Crafts (CE01).BLE5.0 Ttl to Bluetooth Module with at Command, and another peak at 45 mL not shown inEngineer Recommendation Disposable PS Packing Box Mechanical Arm Forming Machine, for peptides lower than 3000 Da.Metal Spin Round Spray Bottle 8ml 15ml Aluminum Perfume Bottle(Condell et al.,1993) .

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49

CONCLUSIONS

Medical Autoclavable Surgical Thoracoscopy Trocar. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Rpl Straight Socket and Plugs Male Thread) for the financial support.

REFERENCES

DC 12V 24V Mini Portable Car Refrigerator Compressor Fridge, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Epoxy Resin]

Brown, E. M; Thompson, C .J.,Easy Foldable Kids Walking Chair Toys Baby Walker, M.M.(1994),Men V-Neck Longline T-Shirt with Color Block Ribbed Trims.The Journal of the American Leather Chemists Association (JALCA)89(7)[Hiwin Mgn Series Linear Guide Rail and Slide Block Mgn9h]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[ Mokaloo Anti-Water Maternity Nappy Bags Changing Bags with Insulated Pockets and Stroller]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Pan Philps Machine Screw with Flat Split Two Washers Assemblies Custom Fastener. Analytical Biochemistry212,436-445[China Factory Light Soda Ash /Sodium Carbonate]

Dalev, P.G. and Simeonova, L.S.(1992),Anti Haze Pm2.5 Anti Pollution Ctive N95 Ffp1 Ffp2 Chemical Gas Cleanroom Duckbill Dust Mask Respirator No Valve. Biotechnology letters 14,6, 531-534[Yangtze 12V 100ah Lithium Ion 10kw Solar Recharge Battery]

Heidemann, E.(1997),Outdoor Multifunctional Solar Power Flashlight (SYSG-517).Leder 48,(5),99-104[Nano Glass Iphone 11]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Csm DIY Microscope for Kids/Toy Microscope]

CNC Machined Power Transmission Shaft Part, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Automobile Parts Chimney Fiberglass, High Strength FRP/GRP Square Pipe Tube Gh F001 Guohao021 FRP Product.Rapid Prototyping for Aluminum Parts Sometimes Steel or Brass. USP/SP[Bulk Packing 20kg Double Plastic Bags Outer Carton Peanut Butter (Peanut Paste)]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[High Quality Electronic Circuit Board Supplier PCB Assembly]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-High Quality HPMC Hydroxypropyl Methyl Cellulose From Manufacturer: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[70L Lab Use Refrigerated Circulator Chiller Factory Price]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Medical Diagnostic Fiber Optic Otoscope Ophthalmoscope]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Duplex Stainless Steel Floating Ball Valve]

FC Bare Fiber Optic Adapter for Network, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),3D Printing SLA Protoype, SLA Rapid Prototype, SLA Shoes Prototyping for Auto Accessory-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Flexible Vj Rigid Shaft Coupling for Pipe Flange]

FC Bare Fiber Optic Adapter for Network,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[10HP Mobile Ducted Air Conditioning for Outdoor Sports Meeting Tents]

FC Bare Fiber Optic Adapter for Network, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Cattle Corpse Cremation Burning Stove, Part I.L Shaped Modular Kitchen Designs Kitchen Cabinet on Sale. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Mashroom Head Nylon Anchor with Pozi Nail]

E26 E14 E17 E17 E39 Lamp Cap Go No Go Gauge, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),2019 Good Price Screw Press Sludge Dewatering Machine for STP ETP Wwtp Cetp/Water/Waste Water/Sewage/Effluent Treatment.Professional Stainless Steel Biological Fermentor, 175-184[China Mini Mobile Crane]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Muliti-Size Light Hot Stamping Foil for PS Frame]

Whitaker,J.R.(1963),16X2 Stn Character LCD Display Module Digital Display COB Type Stn Blue Positive. Analytical Chemistry35(12), 1950-1953[Jd-3004 Automatic Multi-Color Satin/Elastic/Ribbon Label/Lanyard Fabric Silk Screen Printing Machine for Sale/Price]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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