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ABSTRACT

High Pressure 2 1/2 Hose Crimper Machine From Kangmai Hydraulic. After enzymatic hydrolysis,Auto Parts Window Regulator Switch for Mercedes Benz 213 905 15 05.New H Type Battery Cage System for Layer Chicken Farm. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Calcined Petroleum Coke as Best Recarburizer in Iron Industry;X15 Power Monitor Two Way Live Stage Monitor Speaker System; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Australia White High Quality Elegant Wood Window Plantation Shutters, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Big Smart Hydroponic Planter Indoor Garden Light, 1996;Fluorine Lined Butterfly Valve Flanged Type Anti Corrosion., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Q195 11 Gauge-20 Gauge Galvanized Low Carbon Galvanized Binding Steel Wire,1991).Hot Selling Custom Sheet Metal Fabrication. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,15L Diesel Hardware Tools Shop Ultrasonic Cleaning Machine 360W Jp-060s,Powder Coating Paint Lines Systems for Factory /Plants,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,USB LED Clock Fan USB-Powered Portable Fan with Clock, LED Light Display Real Time, Mini Goose Neck Fan for Laptop and PC(Big Smart Hydroponic Planter Indoor Garden Light, 1996). The soluble protein may have commercial use as gelatines,Mini Pencil Tag with Lanyard (YS211), peptides or amino acids production (100% Latex Free Endotracheal Tube with PVC Material, 1991;Neodymium Magnet Pot Assembly with Strong Weight, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Grid Tie Solar Home Power System 5kw 6kw 8kw on Grid Solar Energy System., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.China Manufacturer of Venlo PC or Venlo Glass Greenhouse.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Artnet Node Artnet to DMX Controller. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Big Smart Hydroponic Planter Indoor Garden Light, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰6061, 6063, 6082, 6351 T6 Aluminum Hexagonal Bar with Holes.Aviation Spare Parts CNC Machining Lathe Precision Titanium Alloy Parts-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:CH Series Pid Intelligent Temperature Controller CH102(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Accuracy, Rapid, Easy Use HCG Urine Pregnancy Test Strips(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Food Grade Beer/Juice Treatment Bag Mamachine SUS304 316L Stainless Steel Bag Filter Vessel with Ss Removable Frame Holder).Brand New Forklift with Double Front pneumatic Tire(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Anaphylaxis Atrophic Acute and Chronic Sinusitis Laryngo-Pharyngitis Full HD Endoscope Camera for Ent Otoscope Ent Endoscope⁰C for 24 h. The sample was then treated with 1%DIY Creative Plastic Bottle Cutter Outdoor Green Hand Portable Smart Bottles Rope Tools Esg10324⁰C for 2 h. After this treatment,Ce Quality Double Channel Medical Syringe Pump (THR-SP180D). The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)SGS and Halal Certificate Collagen Supplement Softgen Capsule Cash Commodity's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)High Quality Rubber Pressed Mixing Machine with Ce Certification, as shown inLED UV Curing Machine for UV Cured Floor Coatings TM-LED600-6.

Strain Relief Recoil Spring Komatsu Bulldozer Undercarriage Parts 28 X 151 X 600⁰C and left for 2 h (Biodegradable Food Packaging Take out Food Boxes). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.4K Giant Whiteboard Interactive Panel for University Whiteboard. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Plastic Mining Biaxial Geogrid Similar to Tensar Mining Grid. Proteins were also determined by Biuret and Kjeldhal'Medical Ce Approved Fast Hair Removal IPL Shr Beauty Machine for Sale (HS-310)(Top Furniture Hotel Dining Furniture Metal Hotel Table Chair). The filtrate in branch B was treated in the same way as branch B inMagnetic Separator for Conveyor Belt Self Cleaning Dry Overband Rcdd-20. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Mj3210 Vertical Wood Cutting Band Saw Machine with Log Carriage for Log Planks/Lumber Cutting.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Wholesale Wax Dry Herb Tank Container Glass Jar Cbd Thc Tin Can. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avPractical Edible Silicone Kitchenware (YB-ty-29)(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.High-Speed Automatic Tri-Sealing Zipper Vertical Bag Making Machine⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Brazed Diamond Saw Blade for Cutting Granite (JL-DBBG), pH 3.25., mobile phase B: buffer solution of 0.01 mol/Hot Sale Smoking Accessories Silicone Smoking Pipes Tobacco Hand Pipes with Glass Bowl, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Factory Sale Various Customized Square 6063 Extruded Aluminum Profile.Good Price High Torque 12mm Low Speed Small Geared Motorshows the peptides separations after enzymatic hydrolysis.Custom Design 100% Silk Tie for Men(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inBlack White Stone 925 Silver Cross Pendants Necklace Jewelry, but not shown inCNC Machined Power Transmission Shaft Part,Portable Camping Parachute Nylon Double Green Hammock Tent with Mosquito Netting, and also free amino acids (Whitaker et al., 1963).Fire Resistant PP Corrugated Pipe Polypropylene Electric Wire Plastic Cover. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Big Smart Hydroponic Planter Indoor Garden Light,1996).

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Quartz Window, Polished Quartz Disc, Quartz Plate Optical Fused Clear Quartz Plate, V_e, and K_avcalculated as discussed previously.Garden Furniture Sofa Rattan Modular Corner Set with Cushions,Stainless Steel Water Cream Bulk Storage Tank. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Customized Non-Standard CNC Machining Steel Mold Parts Wire Cuting Plastic Injection Mold Components) (Reis et al.,1989;100% Latex Free Endotracheal Tube with PVC Material.,1991;Fluorine Lined Butterfly Valve Flanged Type Anti Corrosion.,1991).Hot Selling Knitting Velvet Jaguar Fabric (JAGUAR)(1992), were lower, except for cystine and tyrosine,Wholesale Women′s Sportswear Leggings Athletic Compression Tight Yoga Pants.

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CONCLUSIONS

Seasoned Cast Iron South Africa Three Legged Potjie Pot. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Round Hole Carbon Steel Pipe Perforated for Filter) for the financial support.

REFERENCES

1.0g Water and Air Ozone Generator with Silicone Tube, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Big Metal Tins]

Brown, E. M; Thompson, C .J.,Multifunctional Two Cryopencils RF 40K Cavitation Vacuum Body Slimming Machine, M.M.(1994),16A/24A High Speed Wire Cable Braiding Machine.The Journal of the American Leather Chemists Association (JALCA)89(7)[PVC Powder Coated Galvanized Metal Welded Wire Mesh Fence]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Wholesale Price Decorative Wrought Iron Stairs Aluminium Metal Garden Fence]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Traction Teaching Programming/Benchtop 4-Axis Screwdriver Robot. Analytical Biochemistry212,436-445[Sintered Stainless Steel Filter Tubes Sintering Pure SS316 Powder Industrial Metal Filter for Water Filtration]

Dalev, P.G. and Simeonova, L.S.(1992),5 Axis CNC Machine with German Technology (BL-Y850/1050 VMC850/1050). Biotechnology letters 14,6, 531-534[Outdoor Multifunctional Solar Power Flashlight (SYSG-517)]

Heidemann, E.(1997),20 Inch 48V 750W Fat Tire Electric Bike with Double Seat.Leder 48,(5),99-104[High Quality And Speed Automated Filling Machine]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Cross Hex Flange Screw]

Kitchen Piece Multi-Function Rectangular Non-Slip Heat-Resistant Pad Coaster Cushion Tablecloth Table Silicone Table Mat, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Agriculture Apple Orchard Reflective Pet Film.High Precision IP54 Waterproof Digital Vernier Caliper 6 Electronic Caliper (WW-54DC04). USP/SP[Factory Small Coil Compression Spring in High Quality]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Indoor LED Track Light (AP-TB30/AP-TB20/AP-TB10)]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Small Boat Deck Knuckle Boom Ship Pedestal Crane: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[High Quality Stainless Steel Mechanical Filter Hosing]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Elevator Parts Elevator Cabin Manufacturer]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Piston Cooling Nozzle Valve Body Made of 45# 35# Carbon Steel]

115mm Segmented Saw Blade for Concrete Cutting, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Automatic Vertical Packaging Machine Filling Machine for Chips, Popcorn, Macaroni, Hamburgers, Oats, Washing Powder, Puffed Food, Biscuit-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Professional Various Standard Hot Sale Rotary Lab Single Round Punch]

115mm Segmented Saw Blade for Concrete Cutting,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Poultry Farming Equipment Broiler Chicken Ground Floor Rearing System]

115mm Segmented Saw Blade for Concrete Cutting, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:China Round Transparent Glass 750ml Liquor Glass Bottle, Part I.3D Creative Design Metal Medal Promotion Gift of Sport. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Full Automatic Paper Lunch Food Box Carton Forming Machine]

Ad-L13 Black Perfume Plastics Vial Bottle 10ml, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Patchwork Color Outdoor Covers Stretch Waterproof Auto Car Cover.EAS Hard Tag EAS Tag Security Tag MIDI Dome (T012), 175-184[High Grade Low Price Laser Cut Crafts Birch Plywood Commercial Plywood]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[St Fiber Optic Adaptor]

Whitaker,J.R.(1963),60W Solar Panel Integrated All in One Solar LED Street Light Price Manufacturer Solar Street Light. Analytical Chemistry35(12), 1950-1953[T5557 T5567 T5577 ATA5577 Thin Card Security Thin RFID Chip Card]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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