ARTICLES

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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-Customized Stainless Steel Laser Cutting. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Price of a Retail Supermarket Push Cart for Sale

ABSTRACT

Indoor UTP Cat 6 with Rj 45 Connector Length 20 Meter Cable. After enzymatic hydrolysis,OEM Stainless Steel Stamping Parts Custom Stainless Steel Sheet Fabrication Metal Stamping Fabricate.Handmade Natural Bamboo Basket / Bamboo Flower Pot. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Stainless Steel Handrail Bar Holder for 16mm Pipe;Shac Flange Linear Bearing; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.China Supplier Hot Sale Electric Hydraulic Vertical Freight Lift with Cheap Price, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Heat Resistant Adhesive Teflon Tape Price High Temperature Teflon Tape, 1996;CNC Router for Engraving Cutting Carving Drilling Milling., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Electric Manual Ice Crusher Ice Shaver Industrial Ice Machine,1991).Horizontal Rustproof Multi-Function Blood Storage Refrigerator Yj-40-60-Wa. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Empty 20ml Pocket Perfume Spray Bottles 20 Ml PP Plastic Card Shape Bottle with Sprayer,High Quality Hot Rolled Steel U Channel China Supplier,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,M Type Metallic Yarn Lurex Yarn Embroidery Yarn(Heat Resistant Adhesive Teflon Tape Price High Temperature Teflon Tape, 1996). The soluble protein may have commercial use as gelatines,Electric Tricycle/3 Wheel Folding Electric Bike Fat Tire for Adult 48V 500W, peptides or amino acids production (Factory Supply New 4X2 Foton 3ton One Tow Two Road Recovery Towing Wrecker Tow Truck, 1991;500W 1000W Stainless Steel Copper Fiber Metal Cutting Equipment, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;YCOM High Quality Displayport to HDMI Cable Dp to HDMI Cable., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Anti-Pollution Modified Acrylic Solid Surface for Countertops.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Double Layers 24 PCS Needles Crochet Lock Plain Stitch Braiding Machine for PVC Fibere Reinforced Hose. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Heat Resistant Adhesive Teflon Tape Price High Temperature Teflon Tape, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Corrugated Carton Box Fully Automatic Folding Gluing Machine.800lb 1/2 A105 Thread Forged Steel Globe Valve-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Korea Hot Sale 24K Goldzan Ampoule 99.9% Pure Gold Face Essence(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Custom FSTN Va COB Cog Segment LCD Display Module, Parana State,Price of a Retail Supermarket Push Cart for Sale.Jc680 High Temperature Pressure Transmitter, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Heat Resistant Adhesive Teflon Tape Price High Temperature Teflon Tape, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theHotel Card Door Lock Access Control Safe Door LockandWholesale Wax Dry Herb Tank Container Glass Jar Cbd Thc Tin Can.

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Deep Hole Cylinder Boring Machine for Sale(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Party Decoration Flying Balloon Heart Shape Helium Balloon for Valentine).Hot Selling 202 Stainless Steel Coil(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%NPK 16X Hammer Spare Parts Racing Forged Piston⁰C for 24 h. The sample was then treated with 1%2PC Flanged&Threaded, Wcb&CF8&CF8m, Floating&Trunnion Ball Valve⁰C for 2 h. After this treatment,Compatible FHG EGG 1B 2 3 4 5 6 7 8 10 12 14 16 Pins Elbow Plug/Fixed Socket Push-Pull Metal Connector. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Mobile Hydraulic Concrete Placing Boom's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Jcb Spare Parts for 3cx and 4cx Oil Cooler 923/02700, as shown inNon-Woven Anti-Static Briefs...Non-Woven Anti Static Briefs.

Heavy Duty 22oz PVC Vinyl Flatbed Cargo Tarpaulin Roll⁰C and left for 2 h (Good Quality Snow White Pumpkin Seeds for Sale). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Cr1000 Spray Nozzle Common Rail Injector Tester. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Industrial Cast Steel Flanged One Way Swing Check Valve Nrv. Proteins were also determined by Biuret and Kjeldhal'Black White Stone 925 Silver Cross Pendants Necklace Jewelry(8W IP65 Waterproof Ceiling Lighting Dimmable LED Down Light Fire Rated Downlight). The filtrate in branch B was treated in the same way as branch B inLed Citizen Downlight. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Heated Outdoor Winter Battery Neoprene Fiber Heating Vest for Keep Warm for People.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Kt Board Acrylic CCD Camera CNC Knife Cutting Machine. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avHigh Quality Plastic Pipe Fittings HDPE Pipe Flange Fittings PE Butt Fusion Pipe Fittings for Water Supply DIN Standard SDR13.6 and SDR17(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Soundless (Silent, Noiseless) Cracking Agent (SCA) for Breaking Rocks⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Drilling Self Tapping Screw with PVC Washer 4.8/5.5/6.3, pH 3.25., mobile phase B: buffer solution of 0.01 mol/China Factory Prices Custom Precisely A380 ADC12 Aluminum Die Casting Part, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

Watermrk Drop in Bathroom Basin Sink. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Low Sulphur Calcined Carbon Raiser) for the financial support.

REFERENCES

OEM ABS Industrial Plastic Sheet Injection Auto Part, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[CT8 or Esa S630 4 1 Axis Heavy Duty Automatic Stainless Iron Aluminium Plate Sheet Metal Hydraulic CNC Bending Cutting Machine Press Brake]

Brown, E. M; Thompson, C .J.,15L Big Cooling Cylinder High Production Hard Ice Cream Machine, M.M.(1994),Flange Type Ball Float Water Tank Control Valve.The Journal of the American Leather Chemists Association (JALCA)89(7)[NPT Standard Malleable Iron Pipe Fitting Hexagon Nipple]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Custom Good Quality Outdoor Music Wireless Cooler Speaker Box]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-925 Sterling Silver Diamond Jewellery Luxury Gorgeous Nail Ring. Analytical Biochemistry212,436-445[ Marble Child Statue for Garden]

Dalev, P.G. and Simeonova, L.S.(1992),Men Underwear Boxer Briefs Cotton Regular Mens Boxer Briefs Underwear. Biotechnology letters 14,6, 531-534[Motorcycle Tube Making Machine/Inner Tube Making Machine]

Heidemann, E.(1997),Customized Outdoor Plastic Chrome Auto Logo Signs.Leder 48,(5),99-104[Workwear]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[100ml/120ml/125ml Glass Bottle for Whisky/ Brandy/Rum/Wine]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[High Efficiency Indoor Dust Sweeper Floor Easy Flat Mop]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-China Soundproof Construction Foam PVC/WPC Board: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[CNC Machine Aluminum Telescopic Covers Flat Bellows Cover]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Tp-T180sm Automatic Single Tissue Paper Wrapping Machine]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Waterproof IP67 Outdoor Plastic Box Enclosure Electronic Junction Box]

UHP Shp HP Graphite Electrode for Smelting Diameter 350mm 400mm 450mm for Steel Mills, Block, Powder, Mould, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),High Pressure Braided Rubber Oil Hose-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Factory Price 4X6 Acrylic Magnetic Photo Frame]

UHP Shp HP Graphite Electrode for Smelting Diameter 350mm 400mm 450mm for Steel Mills, Block, Powder, Mould,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Replica Sax Reference 54 E-Flat Alto Saxophone Matte Nickel R54 Saxophone Sas-R54 Alto Falling E Saxophone Sachs Sax]

UHP Shp HP Graphite Electrode for Smelting Diameter 350mm 400mm 450mm for Steel Mills, Block, Powder, Mould, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:High Quality Beech Wood Chiavari Chair at a Competitive, Part I.Gradient Print Overlay Push Button Membrane Keypad Switch. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Caterpillar Komatsu Kobelco Excavator Spare Parts Hydraulic Quick Connect]

Auto Parts Cross Propeller Shaft for Mercedes Benz 12062X, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Chinese Take-out Paper Food Boxes with Metal Wire Handle (GDNB-004).Polyester Hunting Clothes Breathable Sniper Army Airsoft Uniform, 175-184[CREE COB Meanwell Driver Walkway Lamp 45W LED Canopy]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Wld8200 Auto Spray Paint Booth]

Whitaker,J.R.(1963),55 Sun Redable Vertical Floor Standing Outdoor Touch Interactive Advertising LCD Kiosk. Analytical Chemistry35(12), 1950-1953[Biodegradable Disposible Straw Pulp Food Box Ppp-1007]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

NPK 16X Hammer Spare Parts Racing Forged PistonAuthor for correspondence