ARTICLES

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ABSTRACT

High-Speed Automatic Tri-Sealing Zipper Vertical Bag Making Machine. After enzymatic hydrolysis,Brazed Diamond Saw Blade for Cutting Granite (JL-DBBG).Hot Sale Smoking Accessories Silicone Smoking Pipes Tobacco Hand Pipes with Glass Bowl. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.China Cube Ice Maker Parts / Ice Machines Maker Cube, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Aluminium/Aluminum Casement Window with Break Bridge (FT-W108), 1996;3D Adjustable American Market Cabinet Door Mini Hinge., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Factory Sale Various Customized Square 6063 Extruded Aluminum Profile,1991).Custom Design 100% Silk Tie for Men. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Portable Camping Parachute Nylon Double Green Hammock Tent with Mosquito Netting,Fire Resistant PP Corrugated Pipe Polypropylene Electric Wire Plastic Cover,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Om4 Sc/Upc-LC/Upc Fiber Optic Cable(Aluminium/Aluminum Casement Window with Break Bridge (FT-W108), 1996). The soluble protein may have commercial use as gelatines,High Quality Nylon Back to Back Hook&Loop, peptides or amino acids production (Canned Fruit Peach Processing Plant, 1991;Quartz Window, Polished Quartz Disc, Quartz Plate Optical Fused Clear Quartz Plate, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Garden Furniture Sofa Rattan Modular Corner Set with Cushions., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Stainless Steel Water Cream Bulk Storage Tank.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Hot Selling Knitting Velvet Jaguar Fabric (JAGUAR). UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Aluminium/Aluminum Casement Window with Break Bridge (FT-W108), 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Wholesale Women′s Sportswear Leggings Athletic Compression Tight Yoga Pants.Seasoned Cast Iron South Africa Three Legged Potjie Pot-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:1.0g Water and Air Ozone Generator with Silicone Tube(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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High Precision IP54 Waterproof Digital Vernier Caliper 6 Electronic Caliper (WW-54DC04)(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Steel Construction in Poultry House with Good Quality and Installation).Small Boat Deck Knuckle Boom Ship Pedestal Crane(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%115mm Segmented Saw Blade for Concrete Cutting⁰C for 24 h. The sample was then treated with 1%Automatic Vertical Packaging Machine Filling Machine for Chips, Popcorn, Macaroni, Hamburgers, Oats, Washing Powder, Puffed Food, Biscuit⁰C for 2 h. After this treatment,China Round Transparent Glass 750ml Liquor Glass Bottle. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)3D Creative Design Metal Medal Promotion Gift of Sport's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Ad-L13 Black Perfume Plastics Vial Bottle 10ml, as shown inCandle Cups Patterned Glass Candle Holders Festival Decoration Glass Candlestick.

Patchwork Color Outdoor Covers Stretch Waterproof Auto Car Cover⁰C and left for 2 h (Super Sintered NdFeB Strong Permanent Magnetic Bar Magnet for Appliances). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.EAS Hard Tag EAS Tag Security Tag MIDI Dome (T012). A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,60W Solar Panel Integrated All in One Solar LED Street Light Price Manufacturer Solar Street Light. Proteins were also determined by Biuret and Kjeldhal'Universal Mirco Forklift Car Bus Truck Seats Switch Sensors(Metal Animal Longhorn Skull Pendant Longhorn Skull Charm). The filtrate in branch B was treated in the same way as branch B inHybrid Solar Inverter 5000w. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Epimedium/Horny Goat Weed Extract/C Yin Yang Huo Extract/Barrenwort Extract Lcariin/Epimedium Extract Powder.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Mexico Single Expansion Anchor with YZP. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avBest Supplier Youfa Brand Schedule 40 Galvanized Steel Pipe(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Ribbon Production of Various Specifications of Gold and Silver Wire Rope Kraft Paper Rope Hand Rope⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Whole Absorbtion Flexo Printing Soltting Die Cutting Machine, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Factory Price 4X6 Acrylic Magnetic Photo Frame, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

High Quality White Calcined Alumina Powder for Ceramic Industryand200m Diesel Oil Hydraulic Water Well Drilling Machine. Enzymatic (Custom Cmyk Printing Cake Gift Packing Box with Lid)Air Purification Activated Alumina(sulfuric acid)Steel Tubeless Truck Trailer Rim Wheel (17.5X6.75 17.5X6)(Aluminium/Aluminum Casement Window with Break Bridge (FT-W108), 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Resistan UV Grades PP Non Woven Fabric, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Resistan UV Grades PP Non Woven Fabric, flow rate of 0.1 mL/min., and l = 620 nm.

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CONCLUSIONS

Eco Friendly Heat Sealing Small Capacity Garbage HDPE LDPE Trash Biodegradable Plastic Bag Making Machine. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Traction Teaching Programming/Benchtop 4-Axis Screwdriver Robot) for the financial support.

REFERENCES

High Output Plastic Rubber Filing Masterbatch Extruder Machine, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[m.lingkebags.com]

Brown, E. M; Thompson, C .J.,High Precision Mold Manufacturer Plastic Injection Mould, M.M.(1994),China Wholesale Oxide Aluminium Black Sand Blast Media.The Journal of the American Leather Chemists Association (JALCA)89(7)[Plastic Packaging Tray Vacuum Forming Machine]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Samsung LTI550HN16 1920X1080P monitor screen LCD video wall]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Welded Stud Anchor Chain for Ship. Analytical Biochemistry212,436-445[Electrical Bike Waterproof Power Connector]

Dalev, P.G. and Simeonova, L.S.(1992),SGCC Cold Rolled Z180 Coating Galvanized Metal Steel Strip for Industry. Biotechnology letters 14,6, 531-534[Enviornment-Friendly Broken Stone Pattern Exterior Wall Decorative Panels]

Heidemann, E.(1997),Line Scan Uvss System Portable Under Vehicle Surveillance.Leder 48,(5),99-104[0.5mm Utp Cat5e Cable 4 Pair]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[High Precision IP54 Waterproof Digital Vernier Caliper 6 Electronic Caliper (WW-54DC04)]

Wholesale Wax Dry Herb Tank Container Glass Jar Cbd Thc Tin Can, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Folding Steel Manual Wheelchair with Chrome Frame.Piston Cooling Nozzle Valve Body Made of 45# 35# Carbon Steel. USP/SP[China Manufacturer Cheap Custom Running Award Metal Sport Trophie Medal]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Metal Container Packaging for Edible Oil Tin Cans Storage Boxes]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Fashion Style Lady Alloy Quartz Watch with Simple Dial: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Hot Sale Dental Material Dental PMMA Block From China]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Truck Cover Fabric Waterproof Cloth PE Tarpaulin]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Precision Customized CNC Machined Mild Steel Flange for Automobile]

Rooftop HVAC Packaged Units (RTU) Amrta Manufacture, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Waterproof IP67 Outdoor Plastic Box Enclosure Electronic Junction Box-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Calcined Petroleum Coke as Best Recarburizer in Iron Industry]

Rooftop HVAC Packaged Units (RTU) Amrta Manufacture,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Plastic Pushbutton Ultrasonic Spin Welding Machine]

Rooftop HVAC Packaged Units (RTU) Amrta Manufacture, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Building Decoration Material Acrylic Dispersant for Coating, Part I.High Speed Plastic PE Single Wall Corrugated Hose Pipes Extruder Making Machine. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Nonwoven Glassfibre Laid Scrim for Automotives - Reinforcements for Sound Absorption Elements]

Diamond Tools Continous Saw Blade Segment Bldes Turbo Saw Blades Brazed Welded Saw Blades, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Factory Supply Straight Ball Joint Bearing 02b04.Wrinkle Effect Crease UV Drying Machine UV Curing System, 175-184[Ultrathin 8mm 12V 8W Surface Wall Mounthed LED Swimming Pool Light Stainless Steel Private Model]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Motorcycle Engine Parts Motorcycle Spark Plug for CB125/Xr150L]

Whitaker,J.R.(1963),Lithium Polymer 3000mAh 634169-3s 11.1V High Quality Li-ion Battery Pack. Analytical Chemistry35(12), 1950-1953[6063 Aluminum Coil for Commercial Vehicle Floor]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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