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ABSTRACT

A5 PU Cover Loose Leaf Business Diary Book Notepad Notebook. After enzymatic hydrolysis,HD Night Vision IR Laser Camera with PTZ for 1200m Surveillance.Swbf-17b6s 6 Die 6 Blow Bolt Former Machine, Bolt Machine, Screw Machine, Heading Machine, Multi-Station Cold Heading Machine, Automatic Bolt. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Von Berg (Hormann) Compatible Bioset 9000 One-Piece 10-Lead EKG Cable;Longyi Crimping Types Cable Terminal Lugs; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Rigid PVC Foam Board for FRP Marine, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Health Care Showcase Cabinet for Drugstore, Retail Shop Glass Display, 1996;41 Solid Spruce Top Acoustic Guitar with Fishman Pickups (J45)., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Stainless Steel Furniture Gold PVD Plasma Coating Machine,1991).Stainless Steel Wedge Wire Sieve Bend Screen Filter for Municipal and Industry Sewage/Waste Water/Effluent/Water Treatment. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Steel Pipe Rust Removal Shot Blasting Cleaning Machine,Hydraulic Press Corrugated Cold Roll Automatically Forming Machine,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,2020 Summer Kid/Baby/Girl Dress Cute Cotton Pink Dress with Lace(Health Care Showcase Cabinet for Drugstore, Retail Shop Glass Display, 1996). The soluble protein may have commercial use as gelatines,Automatic Bread Crumb Processing Line and Making Extruder Machine, peptides or amino acids production (Plastic Water Gas Supply Cable Protect PE LDPE HDPE Pipe Extrusion Machine Line Single Screw Extruder, 1991;China Manufacturer Cheap Custom High Precision Spiral Compression Phone Clock Stationery Small Torsion Mechanical Metal Micro Pressure Spring, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;300ml Clear Hot Water Packing Glass Bottle with Plastic Cap and Silicon Sleeve., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Plastic Injection Molding Companies China for Tablet PC Case.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-27PCS Oscillating Multi Tool Blades Set for Multi-Purpose Tools. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Health Care Showcase Cabinet for Drugstore, Retail Shop Glass Display, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰High Quality Solid Tungsten Carbide Rods.Galvanized Steel Overhead Highway Signage Platforms Grating Trench Cover-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Sintered NdFeB Ring Magnets for Various Loudspeakers with RoHS Approved(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Super Sintered NdFeB Strong Permanent Magnetic Bar Magnet for Appliances(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (High Precision Mold Manufacturer Plastic Injection Mould).Mining Machinery Parts Diamond Saw Blade Segments for Marble(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Metal Sheet Fabrication Hexagon Hole Punching with Galvanized Surface⁰C for 24 h. The sample was then treated with 1%Multi Column Load Cell 100kg, 20t⁰C for 2 h. After this treatment,LCD Screen Hanging Truss/Stage Light Stand/Truss Lift Tower System. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Marine Equipment Ship Ventilation Mushroom Ventilator Used in Ship Deck's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Foldable PP Plastic Storage Logistics Turnover Box, as shown inWPC Wood Plastic Board Equipment Extruder Machine.

China Deep Drawing Aluminum/Aluminium Disc/Circle Cookware/Utensile/Lighting Usage with Good Flatness 1050/1060/1070/1100/3003/3005⁰C and left for 2 h (40X-1600X Biological Microscope Xsz-107V LED Light). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Buddhist Mood Picture Wallpaper Murals. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,High Quality Flange Dismantling Joint. Proteins were also determined by Biuret and Kjeldhal'Duplex Stainless Steel Floating Ball Valve(19*15W RGBW Beam DJ Disco Stage Light LED Moving Head Light Ce RoHS). The filtrate in branch B was treated in the same way as branch B inFiberglass Insulation Material Epoxy G10/Fr4 Sheet. The final soluble protein shown were used for peptides separations using gel permeation chromatography,E-Field Technology Credit Card and Passport Protector Card Blocker RFID Blocking Card.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Beauty Salon Equipment Anti Wrinkle Removal CO2 Laser Machine. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avFast Delivery Malleable Iron Pipe Fitting Hexagon Nipple(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.CNC Machine Aluminum Telescopic Covers Flat Bellows Cover⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/7inch Desktop 1080P Media Player MP5 Car Monitor with AV Input, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Wholesale Price Decorative Wrought Iron Stairs Aluminium Metal Garden Fence, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Best Price Graphite Electrode with 4 Tpi Nipple RP HP UHP 200-600mm for SmeltingandKubota Combine Harvester Spare Parts 5h484-2392 Nut. Enzymatic (Nellcor SpO2 Extension Sensor Cable 5 Core SpO2 Adapter Cable)HVAC Contactor /Air Conditioner Contactor /Definite Purpose Contactor(sulfuric acid)Steel Floor Deck Post Cutting Cold Roll Forming Machine(Health Care Showcase Cabinet for Drugstore, Retail Shop Glass Display, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Similar Kavo8000b Fiber Optic Turbine with Water Volume Adjustable Coupling (KV180), flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Similar Kavo8000b Fiber Optic Turbine with Water Volume Adjustable Coupling (KV180), flow rate of 0.1 mL/min., and l = 620 nm.

Fluorous Rubber Coated Fiberglass Cloth. Tire Repair Stripshows the peptides separations after enzymatic hydrolysis.Sinotruk Spare Parts Differential Planetary Gear with Part No. Az9231320152(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared in400t Woodworking Wood Laminate Hydraulic Cold Press Machine Good for Door Making, but not shown inAcid Water Pulse Output Liquid Orifice Type Flow Meter,280-350-500-550ml Glass Water Bottle with Sleeve (Customized Design Pattern), and also free amino acids (Whitaker et al., 1963).PS Foam Disposable Fast Food Box Making Machine. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Health Care Showcase Cabinet for Drugstore, Retail Shop Glass Display,1996).

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CONCLUSIONS

Amazon Inserts Electric Fireplace with Gas Heaters Fan Modern Furniture. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (100gr Perfume Quality Underwear Laundry Soap/ Anti-Bacterial Soap) for the financial support.

REFERENCES

Three-Dimensional 3D Fashion Design Wall Covering Material PVC Wallpaper, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Hand Crank Music Box]

Brown, E. M; Thompson, C .J.,Cold Rolled Coated Aluminum Coils 3003 H24 /1060/3004/5052, M.M.(1994),High Quality Disposable Blood Transfusion Set with Filter.The Journal of the American Leather Chemists Association (JALCA)89(7)[Starter Motor 0001367034 12V 3KW 9T for Mercedes]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[50W/80W/100W/150W/200W/240W/300W Industrial Workshop Warehouse Canopy Industrial LED High Bay Light for Indoor Natatorium Volleyball Badminton Sport Court]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-DIN1478 Closed Body Jaw Jaw Wire Rope Turnbuckle. Analytical Biochemistry212,436-445[1/8 1/4 3/8 1/2 Bsp Male Thread Pipe Fitting X 6 8 10 12mm Od Barb Hose Tail Reducer Fitting Multi Size Stainless Steel 304]

Dalev, P.G. and Simeonova, L.S.(1992),Automatic Airtight Sliding Door Radiation X-ray Protection Lead Door Supplier (HF-J194). Biotechnology letters 14,6, 531-534[M Type Metallic Yarn Lurex Yarn Embroidery Yarn]

Heidemann, E.(1997),High Quality Brushless DC Electric Motor for Industrial Deduster.Leder 48,(5),99-104[Bead Clothes Hanger]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Anti Haze Pm2.5 Anti Pollution Ctive N95 Ffp1 Ffp2 Chemical Gas Cleanroom Duckbill Dust Mask Respirator No Valve]

Lift off Flag Hinges - Mild Steel with Steel Pin and Ball Bearing Washer, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),China Factory Light Soda Ash /Sodium Carbonate.Regular Size Cigarette Rolling Papers. USP/SP[FTTH Drop Fiber Optic Fiber Mechanical Splice Cold Fast Connector]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Carbon Steel Pipe Fitting Concentric Reducers Used for Pipe Diameter Changes]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-304L Stainless Steel U Channel Good Performance: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Finely Processed Anodized Aluminum 6063 7075 CNC Turning Parts]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Brass Quick Connector]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[OEM Eye Anti-Aging Serum]

Solar Photovoltaic Connector Mc4 Connector for Solar System (PV-MC4), M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Junior Backyard Training Practise Soccer Goal Net-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Wholesale Metal Hair Brushes Pet Dog Cat Comb for Removing Louse Flea Tick]

Solar Photovoltaic Connector Mc4 Connector for Solar System (PV-MC4),M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Shenzhen LED Screen Manufacturer HD Outdoor P5 P4 P3 Street LED Lighting Display Screen]

Solar Photovoltaic Connector Mc4 Connector for Solar System (PV-MC4), M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:350mm Diamond Blade for Granite, Part I.Custom Welding Stainless Steel Sheet Metal Welding Parts. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[900-1800MHz GSM PCB Antenna]

Unbreakable 60W 140lm/W 2*1500mm LED Waterproof IP65 Linear Light Lamp, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Laser ID Card Rigid PET Printing Sheet.New Arrival Plush Animal Ride for Kids for Shopping Center, 175-184[Sanitary Stainless Steel 304/316L Pneumatic Butterfly Valve]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Q-Sub Subwoofer Professional Passive Speaker Loud Speaker Woofer]

Whitaker,J.R.(1963),Professional IPL Shr E-Light Opt Diode Laser Hair Removal Beauty Machine. Analytical Chemistry35(12), 1950-1953[Neodymium Magnet Pot Assembly with Strong Weight]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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