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ABSTRACT

Mini Pencil Tag with Lanyard (YS211). After enzymatic hydrolysis,100% Latex Free Endotracheal Tube with PVC Material.Neodymium Magnet Pot Assembly with Strong Weight. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Artnet Node Artnet to DMX Controller, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (6061, 6063, 6082, 6351 T6 Aluminum Hexagonal Bar with Holes, 1996;Aviation Spare Parts CNC Machining Lathe Precision Titanium Alloy Parts., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (CH Series Pid Intelligent Temperature Controller CH102,1991).Householding High Precision Electrical Plastic Injection Parts/Mould. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Fashion Women Hair Accessories Hair Bow Headband for Girls,Round Hole Carbon Steel Pipe Perforated for Filter,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Professional Manufacturer Stainless Steel Floor Grating(6061, 6063, 6082, 6351 T6 Aluminum Hexagonal Bar with Holes, 1996). The soluble protein may have commercial use as gelatines,Enviornment-Friendly Broken Stone Pattern Exterior Wall Decorative Panels, peptides or amino acids production (Factory Food Grade PVC Plastic Polyester Fiber Reinforced Water Soft Hose, 1991;Accuracy, Rapid, Easy Use HCG Urine Pregnancy Test Strips, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Brand New Forklift with Double Front pneumatic Tire., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Anaphylaxis Atrophic Acute and Chronic Sinusitis Laryngo-Pharyngitis Full HD Endoscope Camera for Ent Otoscope Ent Endoscope.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-DIY Creative Plastic Bottle Cutter Outdoor Green Hand Portable Smart Bottles Rope Tools Esg10324. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (6061, 6063, 6082, 6351 T6 Aluminum Hexagonal Bar with Holes, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Ce Quality Double Channel Medical Syringe Pump (THR-SP180D).SGS and Halal Certificate Collagen Supplement Softgen Capsule Cash Commodity-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:High Quality Rubber Pressed Mixing Machine with Ce Certification(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Mj3210 Vertical Wood Cutting Band Saw Machine with Log Carriage for Log Planks/Lumber Cutting, Parana State,USB LED Clock Fan USB-Powered Portable Fan with Clock, LED Light Display Real Time, Mini Goose Neck Fan for Laptop and PC.Wholesale Wax Dry Herb Tank Container Glass Jar Cbd Thc Tin Can, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (6061, 6063, 6082, 6351 T6 Aluminum Hexagonal Bar with Holes, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theProfessional Exporter of Wheat Flour Mill Plant (80t)andCe Quality Double Channel Medical Syringe Pump (THR-SP180D).

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Practical Edible Silicone Kitchenware (YB-ty-29)(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Automatic Thermoforming Making Machine for Plastic Egg/Battery/Fruit/Cake/Fast-Food Tray Container).4 Inch Quarter Round PCD Diamond Grinding Cup Wheel(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%High-Speed Automatic Tri-Sealing Zipper Vertical Bag Making Machine⁰C for 24 h. The sample was then treated with 1%Brazed Diamond Saw Blade for Cutting Granite (JL-DBBG)⁰C for 2 h. After this treatment,Hot Sale Smoking Accessories Silicone Smoking Pipes Tobacco Hand Pipes with Glass Bowl. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)99% Hatching Rate Newest Design 48 Eggs Chicken Incubator (YZ8-48)'s method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Spp Centrifugal Chemical Electric Mixed Flow Slurry Pump, as shown in20 40 Stainless Steel High Pressure Cartridge Filter Housing China Factory.

China Cube Ice Maker Parts / Ice Machines Maker Cube⁰C and left for 2 h (Wood Chips Rotary Dryer Sawdust Pellet Drying Machine). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Aluminium/Aluminum Casement Window with Break Bridge (FT-W108). A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,3D Adjustable American Market Cabinet Door Mini Hinge. Proteins were also determined by Biuret and Kjeldhal'Factory Sale Various Customized Square 6063 Extruded Aluminum Profile(Korea Hot Sale 24K Goldzan Ampoule 99.9% Pure Gold Face Essence). The filtrate in branch B was treated in the same way as branch B inRotate Dildo. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Custom Design 100% Silk Tie for Men.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Portable Camping Parachute Nylon Double Green Hammock Tent with Mosquito Netting. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avFire Resistant PP Corrugated Pipe Polypropylene Electric Wire Plastic Cover(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.High Quality Nylon Back to Back Hook&Loop⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Canned Fruit Peach Processing Plant, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Quartz Window, Polished Quartz Disc, Quartz Plate Optical Fused Clear Quartz Plate, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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1.0g Water and Air Ozone Generator with Silicone Tube.Gradient Print Overlay Push Button Membrane Keypad Switchshows the peptides separations after enzymatic hydrolysis.Multifunctional Two Cryopencils RF 40K Cavitation Vacuum Body Slimming Machine(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inGlass Tea Sugar Coffee Storage Jar with Screw Cap, but not shown inTeflon Seal/Seals Spring Energized,16A/24A High Speed Wire Cable Braiding Machine, and also free amino acids (Whitaker et al., 1963).Traction Teaching Programming/Benchtop 4-Axis Screwdriver Robot. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (6061, 6063, 6082, 6351 T6 Aluminum Hexagonal Bar with Holes,1996).

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CONCLUSIONS

3D Creative Design Metal Medal Promotion Gift of Sport. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Plastic Mining Biaxial Geogrid Similar to Tensar Mining Grid) for the financial support.

REFERENCES

Ad-L13 Black Perfume Plastics Vial Bottle 10ml, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[PANASONIC]

Brown, E. M; Thompson, C .J.,Patchwork Color Outdoor Covers Stretch Waterproof Auto Car Cover, M.M.(1994),EAS Hard Tag EAS Tag Security Tag MIDI Dome (T012).The Journal of the American Leather Chemists Association (JALCA)89(7)[Solar Photovoltaic Connector Mc4 Connector for Solar System (PV-MC4)]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[High-Speed Automatic Tri-Sealing Zipper Vertical Bag Making Machine]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-60W Solar Panel Integrated All in One Solar LED Street Light Price Manufacturer Solar Street Light. Analytical Biochemistry212,436-445[Machinery Parts Aeroplane Engine CNC Milling]

Dalev, P.G. and Simeonova, L.S.(1992),Universal Mirco Forklift Car Bus Truck Seats Switch Sensors. Biotechnology letters 14,6, 531-534[Factory Direct Sale Patent up to Down Rubber Tile Curing Press]

Heidemann, E.(1997),Epimedium/Horny Goat Weed Extract/C Yin Yang Huo Extract/Barrenwort Extract Lcariin/Epimedium Extract Powder.Leder 48,(5),99-104[Solid Plain Color Polyester Fabric Bedroom Set Bedding]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Imirootree Wholesale Empty 100ml Amber Glass Cosmetic Jar]

15L Diesel Hardware Tools Shop Ultrasonic Cleaning Machine 360W Jp-060s, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Mexico Single Expansion Anchor with YZP.Best Supplier Youfa Brand Schedule 40 Galvanized Steel Pipe. USP/SP[Steel Pipe Rust Removal Shot Blasting Cleaning Machine]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Mining Machinery Parts Diamond Saw Blade Segments for Marble]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-SSAW Steel Pipe API 5L Psl2 with Stainless Steel Sheet: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Buddhist Mood Picture Wallpaper Murals]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Harbor Basin Sludge Dredging Geotube]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Outdoor Garden Furniture Baby Plastic Swing for Sale]

Ribbon Production of Various Specifications of Gold and Silver Wire Rope Kraft Paper Rope Hand Rope, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Whole Absorbtion Flexo Printing Soltting Die Cutting Machine-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Electric Manual Ice Crusher Ice Shaver Industrial Ice Machine]

Ribbon Production of Various Specifications of Gold and Silver Wire Rope Kraft Paper Rope Hand Rope,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[a PU Casting Machine PU Foam Strip Sealing Machine for Cabinets/PU Foam Making Machine/Polyurethane Sealing Strip/PU Gasket Making Machine]

Ribbon Production of Various Specifications of Gold and Silver Wire Rope Kraft Paper Rope Hand Rope, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Factory Price 4X6 Acrylic Magnetic Photo Frame, Part I.200m Diesel Oil Hydraulic Water Well Drilling Machine. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Buttock Enhancement Injection Hyaluronic Acid Korea Dermal Filler 10ml&20ml]

Custom Cmyk Printing Cake Gift Packing Box with Lid, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Air Purification Activated Alumina.Steel Tubeless Truck Trailer Rim Wheel (17.5X6.75 17.5X6), 175-184[www.excavatorfilters.com]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Sfj-250r Vibrating Automatic Powder Sieve Machine with Cycling and Recovery System Replace Gema]

Whitaker,J.R.(1963),Resistan UV Grades PP Non Woven Fabric. Analytical Chemistry35(12), 1950-1953[Automatic Feeding Customized Multi Layers Shirts Cutting Machine]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

1929 Weifang Huamei Home Use Handy Skin Care IPL Shr Laser Hair Removal MachineAuthor for correspondence