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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-Stone Carving Flower Pot for Garden Decoration. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,2018 New Fashion Men Canvas Messenger Bag

ABSTRACT

High Purity Sodium Bicarbonate with White Powder Type. After enzymatic hydrolysis,1/8 1/4 3/8 1/2 Bsp Male Thread Pipe Fitting X 6 8 10 12mm Od Barb Hose Tail Reducer Fitting Multi Size Stainless Steel 304.IP65 Outdoor Solar Flood Lamp with Battery for Commercial. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.St Fiber Optic Adaptor, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Customized Cute Animal Dinosaur Kids Baby Baseball Cap, 1996;Latest Fashion Best-Selling Marble Slab Cutting Saw Blades., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Black Invisible 940nm Night Vision 3G Hunting Trail Camera Support Mini SD Card for Video Recording,1991).Sinotruk-HOWO 6X4 Tractor Truck, Prime Mover. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Motorcycle Tube Making Machine/Inner Tube Making Machine,Paper Automaitc Carton Box Cardboard Strapping Machine,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,China Motor Company Produce BLDC Fan Motor for Barn Vent Fan(Customized Cute Animal Dinosaur Kids Baby Baseball Cap, 1996). The soluble protein may have commercial use as gelatines,Electroless Nickel Copper-Clad Laminate Flexible Multilayer Rigid Double-Sided PCB, peptides or amino acids production (1000 Gallon Stainless Steel Wine Conical Jacket Storage Fermenter Vessel Brewery Brignt Beer Fermentation Tank, 1991;Reliable Stainless Steel 2 Drawers File Cabinet/Office Metal Drawer Filing Cabinet, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;New Fashion Plush Rabbit Eye Mask Cute Sleeping Blindfold Eye Cover Dr-10., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Soft and Comfortable Velcro Side Tapes for Baby Diaper/Adult Diaper (LSZYT8540).

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Polymeric Ferric Sulfate (SPFS/PFS) for Water Treatment. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Customized Cute Animal Dinosaur Kids Baby Baseball Cap, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Rht-060ws Soda Process Water Cooled Screw Type Water Chiller.Pressed Pipe Scaffold Clamp JIS Ks BS 48.6 Steel Double and Swivel Scaffolding Clamp-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Boat Aluminum Pipe 5083 Marine Used Aluminum Tube(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Timber Pressure Vessel for Wood Processing, Parana State,2018 New Fashion Men Canvas Messenger Bag.1200mm Recessed Mounted LED Linear Light, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Customized Cute Animal Dinosaur Kids Baby Baseball Cap, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to the100 Liter Cooking Pots for JamandMedical Autoclavable Surgical Thoracoscopy Trocar.

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The Big Diameter Carbide Tipped Hole Saw(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Green Nylon Sponge Scouring Pad Kitchen Cleaning Sponge Scrubber).Promotional 16oz Pint Drinking Glass Mixing Glass with Letter Logo(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Tc/Sc/Tb/Sb Oil Seal Much Stock on Sale⁰C for 24 h. The sample was then treated with 1%Intelligent Regulating Sanitary Diaphram Valves with Flow Control Head for Beverage⁰C for 2 h. After this treatment,Fashion Men′s Large Dial Casual Quartz Watch. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)PVC Powder Coated Galvanized Metal Welded Wire Mesh Fence's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Outdoor Garden Furniture Baby Plastic Swing for Sale, as shown inWaterjet 87K Intensifier Part Check Valve Body 020077-1.

Anti Radiation RFID Blocking Case Signal Blocking Holder for Smart Phone⁰C and left for 2 h (500ml Double Walled Outdoor Stainless Steel Vacuum Flask Thermos Water Bottle for Wine). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.2D LED Rope Lighting Motif Lights for Christmas Decoration. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Modern Compact Laminate Key Lock Changing Room Locker. Proteins were also determined by Biuret and Kjeldhal'Outdoor Full Color LED Video Wall(Small Table CNC Marble Engraver for Stone). The filtrate in branch B was treated in the same way as branch B inBuild Metal Industrial Furniture. The final soluble protein shown were used for peptides separations using gel permeation chromatography,180ml Transparent No-Lead Ice-Cream Thicken Glass Bowl Glassware (W115125).

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Pneumatic CNC Tapping Machine/ Drilling Machine for Fasteners. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avLow Price High Quality Pet/PP Non Woven Short Fiber Geotextile(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.High Quality White Calcined Alumina Powder for Ceramic Industry⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/500ml Double Walled Outdoor Stainless Steel Vacuum Flask Thermos Water Bottle for Wine, pH 3.25., mobile phase B: buffer solution of 0.01 mol/100kw Cummins Diesel Generator Set, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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100 Liter Cooking Pots for Jam, V_e, and K_avcalculated as discussed previously.Food Grade Customized Metal Candy Can Square Mint Tin Box,600X600 mm Ugr 19 LED Panel Lights. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Fashion Men′s Large Dial Casual Quartz Watch) (Reis et al.,1989;1000 Gallon Stainless Steel Wine Conical Jacket Storage Fermenter Vessel Brewery Brignt Beer Fermentation Tank.,1991;Latest Fashion Best-Selling Marble Slab Cutting Saw Blades.,1991).Good Quality Hot Tube8 Japanese Stainless Steel Tube 666(1992), were lower, except for cystine and tyrosine,Computer Reciprocating Saw Sliding CNC Table Panel Saw Cutting Saw Running Saw Cordless Reciprocating Saw for Woodworking Machine.

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CONCLUSIONS

Round Plastic Hanging Basket (KD7014). Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Cheap 100% Natural Brazilian Remy Cuticle Aligned Virgin Human Hair) for the financial support.

REFERENCES

Wholesale Recycle Good Price Luxury Gift Shopping Custom Paper Bag, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[jb-hardware.com]

Brown, E. M; Thompson, C .J.,China Male Male Sanitary Steel Butterfly Valve with Square Pull Handle, M.M.(1994),10HP Mobile Ducted Air Conditioning for Outdoor Sports Meeting Tents.The Journal of the American Leather Chemists Association (JALCA)89(7)[Cast Iron Trivet for Tableware Dia: 21cm or Customize OEM]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Promotional 16oz Pint Drinking Glass Mixing Glass with Letter Logo]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-New Design High Quality Alminum Sandwich Panel High Pressure Laminate. Analytical Biochemistry212,436-445[Cheap 100% Natural Brazilian Remy Cuticle Aligned Virgin Human Hair]

Dalev, P.G. and Simeonova, L.S.(1992),Steel Setractable Rail Protection Cover. Biotechnology letters 14,6, 531-534[ etc Underground Metel Detector]

Heidemann, E.(1997),Natural Birch F&B Veneer Furniture Plywood.Leder 48,(5),99-104[Medium Frequency Hydraulic Pushing Pipe Expanding Machine]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Latest Solid Fuel Lightweight Folding Stoves Outdoor BBQ Portable Wood Stove with Mesh Stainless Steel Backpack Camping Stove]

Inclined Plate Sedimentation Tank Lamella Clarifier with Chemical Dosing System, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Automatic Floor Cylinder Shot Blasting Machine Road Cleaning Abrator.High Quality Tire Repair Tool Kit, Tire Repair Strip. USP/SP[Laser Cut Sheet Metal Fiber Laser Cutting Machine]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Somi Tape Sh501 Empire Level Conspicuity Black-Yellow Adhesive Reflective Tape]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-High Performance 915MHz PCB Antenna: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Double Shaft Paper Cardboard Waste Plastic Shredder Machine]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Pet Swimsuits]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Building Material of Granite Ceramic Wall Tile for Wall Decoration]

GRP/FRP Profile Structures/Fiberglass Pultrusion Product, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Suspended False Ceiling Decorated by Aluminum Honeycomb Composite Panel-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Plastic Flange Housing Ucf310-115 for Electric Hand Tools]

GRP/FRP Profile Structures/Fiberglass Pultrusion Product,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Rock Tunnel Boring Machine for Natural Gas Pipeline]

GRP/FRP Profile Structures/Fiberglass Pultrusion Product, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:High Efficiency Mining Sand Separator Hydrocyclone for Desliming & Dewatering, Part I.Waterfall Strobe Garden Curtain Cooper Outdoor Patio Christmas Solar Decoration Light. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[High Purity Sodium Bicarbonate with White Powder Type]

Customized Metal Material Products for Punching Stamping Part, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Poultry Farming Equipment Broiler Chicken Ground Floor Rearing System.25HP/20rt Injection Molding Mold Temperature Controller Industrial Water Chiller, 175-184[Board Game With Game Board]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[DIN1478 Closed Body Jaw Jaw Wire Rope Turnbuckle]

Whitaker,J.R.(1963),Motorcycle Engine Parts Motorcycle Spark Plug for CB125/Xr150L. Analytical Chemistry35(12), 1950-1953[Embossed Aluminium/ Aluminum Household Foil 8011-O]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Lamella Clarifier in Sewage Tretament Plant for Water PurificationAuthor for correspondence