ARTICLES

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ABSTRACT

Hot Selling Knitting Velvet Jaguar Fabric (JAGUAR). After enzymatic hydrolysis,Wholesale Women′s Sportswear Leggings Athletic Compression Tight Yoga Pants.Seasoned Cast Iron South Africa Three Legged Potjie Pot. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.16A/24A High Speed Wire Cable Braiding Machine, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Traction Teaching Programming/Benchtop 4-Axis Screwdriver Robot, 1996;5 Axis CNC Machine with German Technology (BL-Y850/1050 VMC850/1050)., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (20 Inch 48V 750W Fat Tire Electric Bike with Double Seat,1991).Agriculture Apple Orchard Reflective Pet Film. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,High Precision IP54 Waterproof Digital Vernier Caliper 6 Electronic Caliper (WW-54DC04),Small Boat Deck Knuckle Boom Ship Pedestal Crane,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,115mm Segmented Saw Blade for Concrete Cutting(Traction Teaching Programming/Benchtop 4-Axis Screwdriver Robot, 1996). The soluble protein may have commercial use as gelatines,Automatic Vertical Packaging Machine Filling Machine for Chips, Popcorn, Macaroni, Hamburgers, Oats, Washing Powder, Puffed Food, Biscuit, peptides or amino acids production (China Round Transparent Glass 750ml Liquor Glass Bottle, 1991;3D Creative Design Metal Medal Promotion Gift of Sport, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Ad-L13 Black Perfume Plastics Vial Bottle 10ml., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Patchwork Color Outdoor Covers Stretch Waterproof Auto Car Cover.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-EAS Hard Tag EAS Tag Security Tag MIDI Dome (T012). UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Traction Teaching Programming/Benchtop 4-Axis Screwdriver Robot, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰60W Solar Panel Integrated All in One Solar LED Street Light Price Manufacturer Solar Street Light.Universal Mirco Forklift Car Bus Truck Seats Switch Sensors-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Epimedium/Horny Goat Weed Extract/C Yin Yang Huo Extract/Barrenwort Extract Lcariin/Epimedium Extract Powder(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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200m Diesel Oil Hydraulic Water Well Drilling Machine(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Cixi Kent Factory High Quality Inch Deep Groove Ball Bearing R10 R10zz R4 R188 R168 R3a R3 R166 R156 Use in Inch Bearing Place).Custom Cmyk Printing Cake Gift Packing Box with Lid(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Air Purification Activated Alumina⁰C for 24 h. The sample was then treated with 1%Steel Tubeless Truck Trailer Rim Wheel (17.5X6.75 17.5X6)⁰C for 2 h. After this treatment,Resistan UV Grades PP Non Woven Fabric. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)New Scba Breathing Apparatus Equipment with Ce Certification's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)FRP Pultruded Pipe or GRP Profile, as shown in200 Tons Press Brake 4000mm CNC Press Brake 200 Tons Hydraulic Press Brake with Bending 8mm.

6090 CO2 Sealed Glass Cooling Laser Cutting Machine⁰C and left for 2 h (16 Spindle Lace High Speed Braiding Machine Textile Machinery). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Aluminum Alloy Die Casting Parts LED Lamp Housing Radiator. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Sexy Women Dress Invisible Zipper Jump Suits Fashion Hidden Zipper. Proteins were also determined by Biuret and Kjeldhal'Outdoor Fitness Equipment Swing (JMH-03)(Hoomall Flowers Mixed Patches Iron on Patches for Clothing DIY Fabric Badge Stickers Embroidered Appliques Clothes Repair Crafts (CE01)). The filtrate in branch B was treated in the same way as branch B inLcd Hmi Touch Screen. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Sheet Metal Distribution Cabinet with High Quality.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%OEM Branded Low Price Disposable Baby Pull up Diapers in Bulk. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avNew Type Automatic Multihead Weigher All-in-One Packing Machine for Rice(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Metal Animal Longhorn Skull Pendant Longhorn Skull Charm⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Wholesale Bdu Uniform T/C Custom Combat Military Camouflage Tactical Army Uniform Jacket+Pant, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Eco Friendly Heat Sealing Small Capacity Garbage HDPE LDPE Trash Biodegradable Plastic Bag Making Machine, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Line Scan Uvss System Portable Under Vehicle Surveillance.16oz Insulated Cardboad Cup Sleeveshows the peptides separations after enzymatic hydrolysis.Folding Steel Manual Wheelchair with Chrome Frame(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inSurface Mounted LED T5 Linear Batten Lamp 0.6m 7W with Switch 3000K Warm White, but not shown in6000 Series Anodizing Extrusion Aluminum Tube,Piston Cooling Nozzle Valve Body Made of 45# 35# Carbon Steel, and also free amino acids (Whitaker et al., 1963).Fashion Style Lady Alloy Quartz Watch with Simple Dial. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Traction Teaching Programming/Benchtop 4-Axis Screwdriver Robot,1996).

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CONCLUSIONS

Best Pinpointer Metal Detector Hand Held Underground Gold Metal Detector. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (SSAW Steel Pipe API 5L Psl2 with Stainless Steel Sheet) for the financial support.

REFERENCES

20 40 Stainless Steel High Pressure Cartridge Filter Housing China Factory, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Aramid Fiber Woven Strap]

Brown, E. M; Thompson, C .J.,Tefude Feeding and Packaging Line for Food with Tray, M.M.(1994),Aluminum Alloy Front Control Panel Machining Monitoring Linear Combination Parts.The Journal of the American Leather Chemists Association (JALCA)89(7)[Oak Veneer Laminated Plywood for Decoration]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Blank Custom Fitness Leggings Women High Waisted Compression Yoga Leggings for Women]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Automatic Sawdust/Rice Husk/Biomass Pellet Making Machine. Analytical Biochemistry212,436-445[12 Inch King Mattress Latex Foam Memory Foam Mattress]

Dalev, P.G. and Simeonova, L.S.(1992),CMP No Nc Metal Switch Black 25mm Illuminated Push Button. Biotechnology letters 14,6, 531-534[Double Door Heated Holding Carts]

Heidemann, E.(1997),Fashion Design Camo Men′s Outdoor Camouflage Cotton Crew Socks, Printed Socks.Leder 48,(5),99-104[Factory Direct Sale Powder Defoamer for Improving Surface Degassing Water Based Paint Antifoam]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Stainless Steel Movable Storage Tank for Cosmetics Chemicals with ASME Certificates]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[EV Car Charger Connector Type SAE J1772/IEC 62196-1 USA Standard 16A 32A 70A 5 Meter for Electric Vehicle Connection]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-13.56MHz Contactless RFID Card MIFARE 1K Card for Prepaid Payment System: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Gear Rack and Pinion for Construction Hoist]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Stainless Steel Oil/Water Moveable Storage Tank]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[High Density Crust Foam Board/Celuka Board]

Thermomolded TPE 5X20mm IP68 10K Air Conditioner Probe Black Cable, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),100% Fiberglass 1*1m Fire Blanket 3732 Fiberglass Cloth-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[240T goodeng water/oil/gas pipe drilling rig HDD machine]

Thermomolded TPE 5X20mm IP68 10K Air Conditioner Probe Black Cable,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Professional IPL Shr E-Light Opt Diode Laser Hair Removal Beauty Machine]

Thermomolded TPE 5X20mm IP68 10K Air Conditioner Probe Black Cable, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:240T goodeng water/oil/gas pipe drilling rig HDD machine, Part I.Mens Messenger Bag 15.6 Inch Waterproof Waxed Canvas. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Wholesale Flower Embroidery Applique Patches]

Colored Silicone NBR O-Rings Seals, Rubber O Ring with High Strength, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Reinforced Stainless Steel 306L Wire Wrap Johnson Type Strainer Screen.Colorful Braided Fabric Cable Round, 175-184[Rh-Ab201 Hospital Bed Series Air Mattress]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Machinery Accessories Custom Foundry Steel Gray / Grey / Ductile Cast Iron Aluminum Sand Iron Casting]

Whitaker,J.R.(1963),Universal Wholesale Hot Sale Custom Blank Keys. Analytical Chemistry35(12), 1950-1953[Customized Outdoor Plastic Chrome Auto Logo Signs]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Portable Camping Parachute Nylon Double Green Hammock Tent with Mosquito NettingAuthor for correspondence