ARTICLES

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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-Tomato Paste Making Machine-Turn Key Solution. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Vegetable Cutter Kitchen Gadget 330ml Mini Size Handy Pull Chopper, String Pulling Food Slicer and Chopper

ABSTRACT

Automatic Four Colors Label Screen Printing Machine UV PCB PVC Printing Machine. After enzymatic hydrolysis,HPMC Similiar to Tylose Powder for Rapid-Set Adhesive.NPT Standard Malleable Iron Pipe Fitting Hexagon Nipple. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;a PU Casting Machine PU Foam Strip Sealing Machine for Cabinets/PU Foam Making Machine/Polyurethane Sealing Strip/PU Gasket Making Machine;Hot Selling Solidwood Viola Wholesale Price Viola; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Building Material Sound Heat Insulation Rock Wool Sandwich Panel, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Sintered Stainless Steel Filter Tubes Sintering Pure SS316 Powder Industrial Metal Filter for Water Filtration, 1996;Ltmg Classic Product 6 Ton Wheel Front Loader for Sale., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Inox Steel Tubes Seamless Pipe 316L 304L Sanitary Ba Tube Mirror,1991).Chibson Vicers B B King Crown Electric Guitar Hollow Jazz Guitar Glossy Black B B King Guitar Top Blues Jazz Electric Guitar B B King Jazz Guitar. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Samsung LTI550HN16 1920X1080P monitor screen LCD video wall,Precision Cold Runner Single Cavity Plastic Injection Mould Auto Parts,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Household Family Medical Emergency Electronic Digital Thermometer(Sintered Stainless Steel Filter Tubes Sintering Pure SS316 Powder Industrial Metal Filter for Water Filtration, 1996). The soluble protein may have commercial use as gelatines,Die Cast Iron Worm Gear Speed Reduce Gearbox, peptides or amino acids production (19*15W RGBW Beam DJ Disco Stage Light LED Moving Head Lighting, 1991;High Quality Couple Watches Steel Material Gift Watch OEM in China, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Glass Tea Sugar Coffee Storage Jar with Screw Cap., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Circular DC Electro Lifting Magnet for Lifting Scraps.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Precision CNC Custom Machining Stainless Steel Aluminum Brass Hardware Parts. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Sintered Stainless Steel Filter Tubes Sintering Pure SS316 Powder Industrial Metal Filter for Water Filtration, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Particle Board Manufacturer, Melamine/Plain Chipboard.CNC Milling Machinery Parts From OEM Manufacturer/ Auto Part-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Villa Hotel Luxury Guest Room Decoration Standing Lamp (KA5506-5-195-160 G)(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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16oz Insulated Cardboad Cup Sleeve(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Stainless Steel Tactile Paving Blind Road Stud).Large Outdoor Simple Gazebo Beer Festival Marquee Tent(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%HOWO Tipper Truck Price with Manufacture Factory Price⁰C for 24 h. The sample was then treated with 1%Wood Fireplace Double Skin Chimney Flue Pipes⁰C for 2 h. After this treatment,High Quality 304 Stainless Steel Sheet for Home Decoration Material. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)T8 20W LED Grow Tube Supplementary Lighting Lamps for Plant's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)LED Rectangular Recessed Linear Lamps for Bank and Car Sale, as shown inMango Pulp Juice Processing Production Line.

Salon Equipment Hair Clipper⁰C and left for 2 h (SS304 316 DIN Standard Ce Stainless Steel Spool Wire for Binding or Weaving). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Flat Conveyor Belt Steel Idler Frame for Roller. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,LED UV Curing Machine for UV Cured Floor Coatings TM-LED600-6. Proteins were also determined by Biuret and Kjeldhal'Custom 30ml Green Dropper Glass Bottle Cosmetic Essential Oil Bottle( Home Fitness). The filtrate in branch B was treated in the same way as branch B inEnig Long Flexible Printed Board Circuit Double Side FPCB. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Durable TPR Pet Molar Toy with Colorful Rope.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Manufacturer Wholesale Adjustable 2mm 5mm Strong Downlight High Torque Brake Pedal Clutch Clothespin Hook Torsion Spring Clip Light Duty Using Flat Wire Spring. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avChromium Oxide Green 99% for Construction and Plastics(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

Zs Round Vibrating Sieve(HPLC)

The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Stainless Steel Tactile Paving Blind Road Stud⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/70L Lab Use Refrigerated Circulator Chiller Factory Price, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Packaging Machine Automatic Weighing Filling Machine (AH-FJJ 100/300/500), pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

Brown /Black /Red Film Faced Plywood Shuttering Poplar Core Waterproof. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (120ml Glass Perfume Bottles for Designer Perfume) for the financial support.

REFERENCES

Competitive Price Sodium Gluconate as Concrete Admixture, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Customized Black EPDM Rubber Brake Diaphragm]

Brown, E. M; Thompson, C .J.,Precision Investment Casting Stainless Steel 304 Wire Rope Hook, M.M.(1994),AC-1325A-20 Automatic Feeding and Cutting Machine Center of Ball Screw for Furniture.The Journal of the American Leather Chemists Association (JALCA)89(7)[Round Panoramic Lift]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Drilling Self Tapping Screw with PVC Washer 4.8/5.5/6.3]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Custom Die Casting Aluminium Product for Connector Usage. Analytical Biochemistry212,436-445[Flange Type Ball Float Water Tank Control Valve]

Dalev, P.G. and Simeonova, L.S.(1992),Stainless Steel Balustrade/Pillar/Column. Biotechnology letters 14,6, 531-534[Men V-Neck Longline T-Shirt with Color Block Ribbed Trims]

Heidemann, E.(1997),Universal-Input Pid Processs and Industrial Digital CH402 Temperature Controller.Leder 48,(5),99-104[Insulation Glass Wool Blanket ASTM Centrifugal Glasswool Felt]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Ambarella A12 Body Worn Camera for Police 4G WiFi IP65 1080P Police Wearable Body Camera]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Packaging Machine Automatic Weighing Filling Machine (AH-FJJ 100/300/500)]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-FPC Hotbar Soldering with Exposed Lead Flex for PCB Soldering: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[High Speed Heavy Duty Customized Radio Shuttle Pallet Racking for Warehouse Storage]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Eggs Trays Packing Machine]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Customized Double Acting Hydraulic Cylinder for Excavator]

Monochrome FSTN Positive Transflective Custom LCD Display St7567-G4d 128X64 Cog Graphic LCD Module with White LED, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Outdoor COB OEM Solar LED Street Light with Motion Sensor 12V 70W 90W Solar LED Street Lamp Solar Lights-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Professional Manufacturer Stainless Steel Floor Grating]

Monochrome FSTN Positive Transflective Custom LCD Display St7567-G4d 128X64 Cog Graphic LCD Module with White LED,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Safety Full Automatic Electric Opening AAC Autoclave for AAC Plant]

Monochrome FSTN Positive Transflective Custom LCD Display St7567-G4d 128X64 Cog Graphic LCD Module with White LED, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Candle Cups Patterned Glass Candle Holders Festival Decoration Glass Candlestick, Part I.Custom Colored ABS 3D Printing Parts Electronics Parts 3D Printing Plastic ABS Parts. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Good Price Channel Steel Used for Machinery]

IS Type Cast Iron Chemical Industrial Pump, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Hot Sale Automatic French Fries Production Line Fresh Potato Chips Making Machine.Custom Grooved Quartz Plate Deep Processing, 175-184[Best Price 304 Stainless Steel Gas Water Orifice Plate Flow Meter in Kaifeng]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[CNC Milling Machinery Parts From OEM Manufacturer/ Auto Part]

Whitaker,J.R.(1963),Hot Sale CNC Router Machine for Wood Cutting (1325). Analytical Chemistry35(12), 1950-1953[PP/Pet Woven Geotextile for Road Construction]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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