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ABSTRACT

Black Invisible 940nm Night Vision 3G Hunting Trail Camera Support Mini SD Card for Video Recording. After enzymatic hydrolysis,Sinotruk-HOWO 6X4 Tractor Truck, Prime Mover.Motorcycle Tube Making Machine/Inner Tube Making Machine. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Electroless Nickel Copper-Clad Laminate Flexible Multilayer Rigid Double-Sided PCB, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (1000 Gallon Stainless Steel Wine Conical Jacket Storage Fermenter Vessel Brewery Brignt Beer Fermentation Tank, 1996;Reliable Stainless Steel 2 Drawers File Cabinet/Office Metal Drawer Filing Cabinet., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (New Fashion Plush Rabbit Eye Mask Cute Sleeping Blindfold Eye Cover Dr-10,1991).Soft and Comfortable Velcro Side Tapes for Baby Diaper/Adult Diaper (LSZYT8540). Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Polymeric Ferric Sulfate (SPFS/PFS) for Water Treatment,Rht-060ws Soda Process Water Cooled Screw Type Water Chiller,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Pressed Pipe Scaffold Clamp JIS Ks BS 48.6 Steel Double and Swivel Scaffolding Clamp(1000 Gallon Stainless Steel Wine Conical Jacket Storage Fermenter Vessel Brewery Brignt Beer Fermentation Tank, 1996). The soluble protein may have commercial use as gelatines,Boat Aluminum Pipe 5083 Marine Used Aluminum Tube, peptides or amino acids production (Imirootree Wholesale Empty 100ml Amber Glass Cosmetic Jar, 1991;2017 Year The Newest 7inch LCD Screen Advertising Brochure Video Greeting Cards, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Cheap 100% Natural Brazilian Remy Cuticle Aligned Virgin Human Hair., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Flanged Braided Metal Hose Connector.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Timber Pressure Vessel for Wood Processing. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (1000 Gallon Stainless Steel Wine Conical Jacket Storage Fermenter Vessel Brewery Brignt Beer Fermentation Tank, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰1200mm Recessed Mounted LED Linear Light.The Big Diameter Carbide Tipped Hole Saw-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Promotional 16oz Pint Drinking Glass Mixing Glass with Letter Logo(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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2D LED Rope Lighting Motif Lights for Christmas Decoration(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (China Quality Heavy Duty Globe Control Valve).Modern Compact Laminate Key Lock Changing Room Locker(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Outdoor Full Color LED Video Wall⁰C for 24 h. The sample was then treated with 1%180ml Transparent No-Lead Ice-Cream Thicken Glass Bowl Glassware (W115125)⁰C for 2 h. After this treatment,Pneumatic CNC Tapping Machine/ Drilling Machine for Fasteners. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Low Price High Quality Pet/PP Non Woven Short Fiber Geotextile's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Quick Connect Camlock and Grooved Couplings Quick Fitting Type D, as shown in2u Lgx Swing out Fiber Optic Patch Panel with 4 SC Simplex Single Mode Adapter Panels.

High Quality White Calcined Alumina Powder for Ceramic Industry⁰C and left for 2 h (Q3210 Vibratory Polishing Shot Blasting Machine). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.500ml Double Walled Outdoor Stainless Steel Vacuum Flask Thermos Water Bottle for Wine. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,100kw Cummins Diesel Generator Set. Proteins were also determined by Biuret and Kjeldhal'Double Wall Glass Vacuum Flask Bottle Stainless Steel Cola Shaped Glass Water Bottle(Building Decoration Material Acrylic Dispersant for Coating). The filtrate in branch B was treated in the same way as branch B inCustomized Poultry Slaughter Equipment 500-10000 Bph for Chicken Abattoir Machine. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Luxury Hotel Furniture Antique Design Marble Side Table.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Ampoule Sealer Machine Hydrogen Oxygen Gas Generator. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avCommercial Double Lane Inflatable Carnival Bungee Run Game(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Indoor Prize Selling Video Toy Story/Arcade Claw/Arcade Game/Coin Pusher /Arcade/Crane/Game /Vending Game/Claw Crane/Crane Claw Machine for Sale⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/800mm Length 600n Load Tension Gas Spring with Metal Eyelet, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Deep Drawing Aluminum Circle /Disc for Cookwares 1050 1060 1100 3003, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

High Quality Tire Repair Tool Kit, Tire Repair Strip. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Fashion Men′s Large Dial Casual Quartz Watch) for the financial support.

REFERENCES

High Performance 915MHz PCB Antenna, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Aroma Diffusers Electronic]

Brown, E. M; Thompson, C .J.,GRP/FRP Profile Structures/Fiberglass Pultrusion Product, M.M.(1994),Suspended False Ceiling Decorated by Aluminum Honeycomb Composite Panel.The Journal of the American Leather Chemists Association (JALCA)89(7)[Men Underwear Boxer Briefs Cotton Regular Mens Boxer Briefs Underwear]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Reinforced Stainless Steel 306L Wire Wrap Johnson Type Strainer Screen]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-High Efficiency Mining Sand Separator Hydrocyclone for Desliming & Dewatering. Analytical Biochemistry212,436-445[Agriculture Apple Orchard Reflective Pet Film]

Dalev, P.G. and Simeonova, L.S.(1992),Waterfall Strobe Garden Curtain Cooper Outdoor Patio Christmas Solar Decoration Light. Biotechnology letters 14,6, 531-534[Tct Circular Saw Blade &Diamond Saw Blade]

Heidemann, E.(1997),Customized Metal Material Products for Punching Stamping Part.Leder 48,(5),99-104[Casting Bronze Foundry]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[5 Axis CNC Machine with German Technology (BL-Y850/1050 VMC850/1050)]

St Fiber Optic Adaptor, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Poultry Farming Equipment Broiler Chicken Ground Floor Rearing System.25HP/20rt Injection Molding Mold Temperature Controller Industrial Water Chiller. USP/SP[Active Demand Plastic 65 Liter Laundry Basket Mould]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Low Density Cleaner in Paper Making Machine]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Motorcycle Engine Parts Motorcycle Spark Plug for CB125/Xr150L: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Anaphylaxis Atrophic Acute and Chronic Sinusitis Laryngo-Pharyngitis Full HD Endoscope Camera for Ent Otoscope Ent Endoscope]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[5mm Clear Float Glass]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Flying Fiber Laser Marking Machine on Plastic Pipe /Metal Pipe]

Cixi Kent Factory High Quality Inch Deep Groove Ball Bearing R10 R10zz R4 R188 R168 R3a R3 R166 R156 Use in Inch Bearing Place, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Theme Park Inflatable Bouncer for Sale (BJ-B16)-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[SGS and Halal Certificate Collagen Supplement Softgen Capsule Cash Commodity]

Cixi Kent Factory High Quality Inch Deep Groove Ball Bearing R10 R10zz R4 R188 R168 R3a R3 R166 R156 Use in Inch Bearing Place,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[High Pressure Hose & Hydraulic Hose SAE 100r1at/ DIN En853 1sn]

Cixi Kent Factory High Quality Inch Deep Groove Ball Bearing R10 R10zz R4 R188 R168 R3a R3 R166 R156 Use in Inch Bearing Place, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Muliti-Size Light Hot Stamping Foil for PS Frame, Part I.Waterproof Garden Yard Decoration LED Flame Solar Glass Jar Light. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[High Quality Plastic Injection Mold Parts Square Interlocks Maker]

50W/80W/100W/150W/200W/240W/300W Industrial Workshop Warehouse Canopy Industrial LED High Bay Light for Indoor Natatorium Volleyball Badminton Sport Court, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),China 1 & 100 RMB Acrylic Currence Frame.1 Ton Toyo Type Manual Chain Block, 175-184[LED Downlight 10W/15W/20W/25W LED Spot Lights Shopping Malls Office Use LED Down Lights Ceiling Light Hotel Spotlight COB LED Light]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[High Quality Suitcase Frame or Profile Bending Machine with Ce Aprroved]

Whitaker,J.R.(1963),Spark Resistant Construction Axial Fan with Fixed Pitch Blade. Analytical Chemistry35(12), 1950-1953[Seasoned Cast Iron South Africa Three Legged Potjie Pot]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Food Waste Composting China Supplier Organic Compost Machine/Organic Fertilizer MachineAuthor for correspondence