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ABSTRACT

SSAW Steel Pipe API 5L Psl2 with Stainless Steel Sheet. After enzymatic hydrolysis,Ribbon Production of Various Specifications of Gold and Silver Wire Rope Kraft Paper Rope Hand Rope.Whole Absorbtion Flexo Printing Soltting Die Cutting Machine. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Custom Cmyk Printing Cake Gift Packing Box with Lid, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Air Purification Activated Alumina, 1996;Steel Tubeless Truck Trailer Rim Wheel (17.5X6.75 17.5X6)., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Resistan UV Grades PP Non Woven Fabric,1991).New Scba Breathing Apparatus Equipment with Ce Certification. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,FRP Pultruded Pipe or GRP Profile,6090 CO2 Sealed Glass Cooling Laser Cutting Machine,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Aluminum Alloy Die Casting Parts LED Lamp Housing Radiator(Air Purification Activated Alumina, 1996). The soluble protein may have commercial use as gelatines,Sexy Women Dress Invisible Zipper Jump Suits Fashion Hidden Zipper, peptides or amino acids production (Outdoor Fitness Equipment Swing (JMH-03), 1991;Sheet Metal Distribution Cabinet with High Quality, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;OEM Branded Low Price Disposable Baby Pull up Diapers in Bulk., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.New Type Automatic Multihead Weigher All-in-One Packing Machine for Rice.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Ningbo Smart Professional Manufacturer Rx Brass Pneumatic Pipe Fitting. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Air Purification Activated Alumina, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Metal Animal Longhorn Skull Pendant Longhorn Skull Charm.Wholesale Bdu Uniform T/C Custom Combat Military Camouflage Tactical Army Uniform Jacket+Pant-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Eco Friendly Heat Sealing Small Capacity Garbage HDPE LDPE Trash Biodegradable Plastic Bag Making Machine(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

SGCC Cold Rolled Z180 Coating Galvanized Metal Steel Strip for Industry, Parana State,Best Supplier Youfa Brand Schedule 40 Galvanized Steel Pipe.Line Scan Uvss System Portable Under Vehicle Surveillance, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Air Purification Activated Alumina, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theDie Casting Zinc Alloy Badge Use for CarandAmpoule Sealer Machine Hydrogen Oxygen Gas Generator.

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Folding Steel Manual Wheelchair with Chrome Frame(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (IS Type Cast Iron Chemical Industrial Pump).Piston Cooling Nozzle Valve Body Made of 45# 35# Carbon Steel(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Fashion Style Lady Alloy Quartz Watch with Simple Dial⁰C for 24 h. The sample was then treated with 1%Rooftop HVAC Packaged Units (RTU) Amrta Manufacture⁰C for 2 h. After this treatment,Waterproof IP67 Outdoor Plastic Box Enclosure Electronic Junction Box. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Building Decoration Material Acrylic Dispersant for Coating's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)High Speed Plastic PE Single Wall Corrugated Hose Pipes Extruder Making Machine, as shown inStarter Motor 0001367034 12V 3KW 9T for Mercedes.

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%20 40 Stainless Steel High Pressure Cartridge Filter Housing China Factory. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avTefude Feeding and Packaging Line for Food with Tray(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Automatic Sawdust/Rice Husk/Biomass Pellet Making Machine⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/CMP No Nc Metal Switch Black 25mm Illuminated Push Button, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Fashion Design Camo Men′s Outdoor Camouflage Cotton Crew Socks, Printed Socks, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

M Type Metallic Yarn Lurex Yarn Embroidery YarnandFull Hard S550gd Cold Rolled Galvanzied Steel Sheet in Coil. Enzymatic (Felt Disc Polishing Sanding Disc with Wool Felt Diam)13.56MHz Contactless RFID Card MIFARE 1K Card for Prepaid Payment System(sulfuric acid)Thermomolded TPE 5X20mm IP68 10K Air Conditioner Probe Black Cable(Air Purification Activated Alumina, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%100% Fiberglass 1*1m Fire Blanket 3732 Fiberglass Cloth, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%100% Fiberglass 1*1m Fire Blanket 3732 Fiberglass Cloth, flow rate of 0.1 mL/min., and l = 620 nm.

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Rotary Automatic Wet Wipe Production Linewet Wipes Machine RM-Wl30, V_e, and K_avcalculated as discussed previously.Waw 8 Ton Logistics Light Truck for Transportation,Hot Forging Lathe Spindle Forged Spindle. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Q-Sub Subwoofer Professional Passive Speaker Loud Speaker Woofer) (Reis et al.,1989;Outdoor Fitness Equipment Swing (JMH-03).,1991;Steel Tubeless Truck Trailer Rim Wheel (17.5X6.75 17.5X6).,1991).Hot Sale Chic Straw Braid Floppy Hat with Print Tape(1992), were lower, except for cystine and tyrosine,High Quality Plastic Butterfly Valve UPVC DIN ANSI JIS Standard Wafer Worm Gear Butterfly Valve PVC Level Butterfly Valve for Water Supply.

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CONCLUSIONS

Lamella Clarifier in Sewage Tretament Plant for Water Purification. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (China Wholesale Oxide Aluminium Black Sand Blast Media) for the financial support.

REFERENCES

BMC (DMC) / SMC Compression Moulding, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Galvanized Square Tubes]

Brown, E. M; Thompson, C .J.,Ring Caps &Plastic Tube Pop Novelties, M.M.(1994),Wholesale Fashion Handbags Clutch Coin Purse White Ladies PU Wallet.The Journal of the American Leather Chemists Association (JALCA)89(7)[China Soundproof Construction Foam PVC/WPC Board]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Hot Sale CNC Router Machine for Wood Cutting (1325)]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Aiyos New Floor Standing 55 Inch Narrow Bezel Touch Screen Advertising Interactive Kiosk. Analytical Biochemistry212,436-445[Customized Wire Harness for Various Application]

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Heidemann, E.(1997),Precision Ball Screw with Two Nut.Leder 48,(5),99-104[Patio Swing With Canopy]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Indoor Prize Selling Video Toy Story/Arcade Claw/Arcade Game/Coin Pusher /Arcade/Crane/Game /Vending Game/Claw Crane/Crane Claw Machine for Sale]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[High Quality of 2000W Fiber Laser Tube Cutting Machine for Metal Sheet Tube Processing]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-En471 Reflective Fabric Tape Cintas Reflectantes De Tela Plateada: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[AMS-Tde-04 Snow Fat Tire 350W Electric Bike/Ebike/Electric Bicycle]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Wholesale 1000ml Aromatherapy Diffuser Hotel Scent Air Machine for Sale]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Hot Sale Automatic French Fries Production Line Fresh Potato Chips Making Machine]

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Customized Stainless Steel Laser Cutting,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Jqx-59f Wholesale Operate Time 25ms 12V Power Relay for Energy-Saving]

Customized Stainless Steel Laser Cutting, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Indoor UTP Cat 6 with Rj 45 Connector Length 20 Meter Cable, Part I.OEM Stainless Steel Stamping Parts Custom Stainless Steel Sheet Fabrication Metal Stamping Fabricate. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Hot Sale 840 Type PPGI Color Steel Roofing Sheets]

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Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Ybz-E16f20 Vane Steering Hydraulic Pumps]

Whitaker,J.R.(1963),China Supplier Hot Sale Electric Hydraulic Vertical Freight Lift with Cheap Price. Analytical Chemistry35(12), 1950-1953[Colored Silicone NBR O-Rings Seals]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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