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ABSTRACT

Carbide Rod H6 Tc Rods. After enzymatic hydrolysis,High Quality Mens Slim Wallet Custom Design Leather Wallet Men.St Fiber Optic Adaptor. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Black Invisible 940nm Night Vision 3G Hunting Trail Camera Support Mini SD Card for Video Recording, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Sinotruk-HOWO 6X4 Tractor Truck, Prime Mover, 1996;Motorcycle Tube Making Machine/Inner Tube Making Machine., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Paper Automaitc Carton Box Cardboard Strapping Machine,1991).China Motor Company Produce BLDC Fan Motor for Barn Vent Fan. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Electroless Nickel Copper-Clad Laminate Flexible Multilayer Rigid Double-Sided PCB,1000 Gallon Stainless Steel Wine Conical Jacket Storage Fermenter Vessel Brewery Brignt Beer Fermentation Tank,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Reliable Stainless Steel 2 Drawers File Cabinet/Office Metal Drawer Filing Cabinet(Sinotruk-HOWO 6X4 Tractor Truck, Prime Mover, 1996). The soluble protein may have commercial use as gelatines,New Fashion Plush Rabbit Eye Mask Cute Sleeping Blindfold Eye Cover Dr-10, peptides or amino acids production (Soft and Comfortable Velcro Side Tapes for Baby Diaper/Adult Diaper (LSZYT8540), 1991;Polymeric Ferric Sulfate (SPFS/PFS) for Water Treatment, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Rht-060ws Soda Process Water Cooled Screw Type Water Chiller., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Pressed Pipe Scaffold Clamp JIS Ks BS 48.6 Steel Double and Swivel Scaffolding Clamp.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Boat Aluminum Pipe 5083 Marine Used Aluminum Tube. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Sinotruk-HOWO 6X4 Tractor Truck, Prime Mover, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Imirootree Wholesale Empty 100ml Amber Glass Cosmetic Jar.2017 Year The Newest 7inch LCD Screen Advertising Brochure Video Greeting Cards-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Cheap 100% Natural Brazilian Remy Cuticle Aligned Virgin Human Hair(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Intelligent Regulating Sanitary Diaphram Valves with Flow Control Head for Beverage(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (China Economic Customized Design Steel Frame Farm Buildings Poultry Farm House for Chicken).Fashion Men′s Large Dial Casual Quartz Watch(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%PVC Powder Coated Galvanized Metal Welded Wire Mesh Fence⁰C for 24 h. The sample was then treated with 1%Outdoor Garden Furniture Baby Plastic Swing for Sale⁰C for 2 h. After this treatment,Anti Radiation RFID Blocking Case Signal Blocking Holder for Smart Phone. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)2D LED Rope Lighting Motif Lights for Christmas Decoration's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Modern Compact Laminate Key Lock Changing Room Locker, as shown inHot Sale High Precision Valve Block Hydraulic Flow Control Valve 1wfl.

Outdoor Full Color LED Video Wall⁰C and left for 2 h (PVDF Exterior Design Curved Aluminum Panel). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.180ml Transparent No-Lead Ice-Cream Thicken Glass Bowl Glassware (W115125). A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Pneumatic CNC Tapping Machine/ Drilling Machine for Fasteners. Proteins were also determined by Biuret and Kjeldhal'Low Price High Quality Pet/PP Non Woven Short Fiber Geotextile(Mini Succulent Planters). The filtrate in branch B was treated in the same way as branch B inThrust Tapered Roller Bearings. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Quick Connect Camlock and Grooved Couplings Quick Fitting Type D.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%High Quality White Calcined Alumina Powder for Ceramic Industry. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_av500ml Double Walled Outdoor Stainless Steel Vacuum Flask Thermos Water Bottle for Wine(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Double Wall Glass Vacuum Flask Bottle Stainless Steel Cola Shaped Glass Water Bottle⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Luxury Hotel Furniture Antique Design Marble Side Table, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Ampoule Sealer Machine Hydrogen Oxygen Gas Generator, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

10HP Mobile Ducted Air Conditioning for Outdoor Sports Meeting Tents. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (1200mm Recessed Mounted LED Linear Light) for the financial support.

REFERENCES

New Design High Quality Alminum Sandwich Panel High Pressure Laminate, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Caterpillar Concrete Parts Main Valve and Relief Valve in Hot Sale]

Brown, E. M; Thompson, C .J.,Steel Setractable Rail Protection Cover, M.M.(1994),Natural Birch F&B Veneer Furniture Plywood.The Journal of the American Leather Chemists Association (JALCA)89(7)[Ad-L13 Black Perfume Plastics Vial Bottle 10ml]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Hot Sale High-Order Elasticty Girls Sports Wear Yaga Suit]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Automatic Floor Cylinder Shot Blasting Machine Road Cleaning Abrator. Analytical Biochemistry212,436-445[Plastic Mining Biaxial Geogrid Similar to Tensar Mining Grid]

Dalev, P.G. and Simeonova, L.S.(1992),High Quality Tire Repair Tool Kit, Tire Repair Strip. Biotechnology letters 14,6, 531-534[3 Years Warranty 3.2V 60ah 75ah 86ah 100ah 110ah 200ah Single LiFePO4 Battery Cell LiFePO4 Lithium Iron Phosphate Battery Cell for EV]

Heidemann, E.(1997),High Performance 915MHz PCB Antenna.Leder 48,(5),99-104[33kv Pole]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Plastic Material Handling Dolly Platform Hand Trolley Truck]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[LED Neon Flex IP67 Waterproof Silicone DIY Neon LED Strip]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-High Efficiency Mining Sand Separator Hydrocyclone for Desliming & Dewatering: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Chromium Oxide Green 99% for Construction and Plastics]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Agate Double Color Pear Pendant Crystal Jewelry Gemstone Necklaces]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Fashion Men′s Large Dial Casual Quartz Watch]

Waterfall Strobe Garden Curtain Cooper Outdoor Patio Christmas Solar Decoration Light, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Customized Metal Material Products for Punching Stamping Part-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[High Quality Plastic Butterfly Valve UPVC DIN ANSI JIS Standard Wafer Worm Gear Butterfly Valve PVC Level Butterfly Valve for Water Supply]

Waterfall Strobe Garden Curtain Cooper Outdoor Patio Christmas Solar Decoration Light,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[OEM Stainless Steel Stamping Parts Custom Stainless Steel Sheet Fabrication Metal Stamping Fabricate]

Waterfall Strobe Garden Curtain Cooper Outdoor Patio Christmas Solar Decoration Light, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Poultry Farming Equipment Broiler Chicken Ground Floor Rearing System, Part I.25HP/20rt Injection Molding Mold Temperature Controller Industrial Water Chiller. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Cast Iron Trivet for Tableware Dia: 21cm or Customize OEM]

Motorcycle Engine Parts Motorcycle Spark Plug for CB125/Xr150L, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Cixi Kent Factory High Quality Inch Deep Groove Ball Bearing R10 R10zz R4 R188 R168 R3a R3 R166 R156 Use in Inch Bearing Place.Theme Park Inflatable Bouncer for Sale (BJ-B16), 175-184[Clear 2mm Float Glass Convex Mirror 305mm*407mm R1200]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Promotional 16oz Pint Drinking Glass Mixing Glass with Letter Logo]

Whitaker,J.R.(1963),Muliti-Size Light Hot Stamping Foil for PS Frame. Analytical Chemistry35(12), 1950-1953[Cheap 100% Natural Brazilian Remy Cuticle Aligned Virgin Human Hair]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

etc Underground Metel DetectorAuthor for correspondence