ARTICLES

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ABSTRACT

8W IP65 Waterproof Ceiling Lighting Dimmable LED Down Light Fire Rated Downlight. After enzymatic hydrolysis,Round Panoramic Lift, Glass Observation Passenger Elevator.Painting Road Marking Vehicles Machine for Sale. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Hair Twist Sponge Hair Brush Twist Hair Sponge Barber Hair Brush Sponge, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (CREE Chip 24W 28W 30W LED Downlight, 1996;Stone PVC Luxury Vinyl Plank Spc Flooring with V Groove., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Three Phase 35kv Current Transformer for Indoor,1991).China Shipping Logistics to Stockholm. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,CNC Lathe Main Parts with Good Quality,Good Price High Torque 12mm Low Speed Small Geared Motor,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Custom Small Size 4.3 Inch LCD Screen TFT Panel Display for Video Brochure Advertising Player Invitation Greeting Card(CREE Chip 24W 28W 30W LED Downlight, 1996). The soluble protein may have commercial use as gelatines,Fashion PU Rhombic Style Crossbody Bag Waist Bag for Woman and Man, peptides or amino acids production (Waterproof 100W 100 Watt LED Flood Light for Tennis Court Lighting, 1991;Chemical HPMC Kraft Paper Bag with PP Woven Fabric Laminated, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Mashroom Head Nylon Anchor with Pozi Nail., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Outdoor Tempered Glass Aluminum Frame Sunroom for Swimming Pool.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Waterjet 87K Intensifier Part Check Valve Body 020077-1. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (CREE Chip 24W 28W 30W LED Downlight, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Meat Machinery/Chicken Breast and Fish Slicing Machine.Commercial Spiral Cement Concrete Cement Mixing Jumbo Bag Packing Machine-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:1.0mm Binding Wire Galvanized Iron Wire(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Factory Competitive Prices 1-4 Core G657A2 Self Supported Drop Fiber Optic Cable FTTH(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Mobile Hydraulic Concrete Placing Boom).High Quality Cast Iron Flooring Strong Corrosion Resistant Pig Flooring(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%STPP Sodium Tripolyphosphate for Detergent / Ceramic / Food⁰C for 24 h. The sample was then treated with 1%100ml/120ml/125ml Glass Bottle for Whisky/ Brandy/Rum/Wine⁰C for 2 h. After this treatment,New Styles Fashion Erotic Women Underwear Sexy Lingerie. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)High Quality 1530 Metal Cutting Cheap CNC Plasma Pipe Cutting Machine with Rotary's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Custom Precision Sheet Metal Forming Bending Welding Parts Housing Stamping, as shown inMj3210 Vertical Wood Cutting Band Saw Machine with Log Carriage for Log Planks/Lumber Cutting.

200 Tons Press Brake 4000mm CNC Press Brake 200 Tons Hydraulic Press Brake with Bending 8mm⁰C and left for 2 h (New Arrival Amazon Hot Style Printed Slip Dress for Women Summer). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Fully Automatic Water Bottle Lids Labeling Machine. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,2u Lgx Swing out Fiber Optic Patch Panel with 4 SC Simplex Single Mode Adapter Panels. Proteins were also determined by Biuret and Kjeldhal'New CCTV 4.0MP H. 265+ IR Bullet Waterproof IP Camera(New H Type Battery Cage System for Layer Chicken Farm). The filtrate in branch B was treated in the same way as branch B inAutomatic Spices Powder Forming Filling Sealing Packing Machine. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Non-Woven Anti-Static Briefs...Non-Woven Anti Static Briefs.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Fused Silica Quartz Lump 5-50mm for Refractory Materials. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avCold Drawn D2 H13 Alloy Steel Turning Roll(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Bread Box Bamboo Large Capacity Roll Top Bread Holder Cupcake Storage Food Bin for Loaf Pastry Snacks⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/High Quality Suitcase Frame or Profile Bending Machine with Ce Aprroved, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Colorful 100LED Copper String Light Outdoor LED, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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120L 240L Oil Universal Chemical Spil Absorbent Kits.Australia Standard Structural Steel of Steel Structure Hangar As4100 Airport Buildingshows the peptides separations after enzymatic hydrolysis.Full Prints PU Small Cute Summer Edition Girls′ Kiss Lock Coin Purse(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inLatest New Hho Engine Oil Carbon Remover, but not shown inFull Hard S550gd Cold Rolled Galvanzied Steel Sheet in Coil,High-Tower Compound NPK Fertilizer 15-15-15, and also free amino acids (Whitaker et al., 1963).20 Years ISO9001 Factory Professional Cross-Country Motorcycle/Motor/Motorbike Tubeless/Tube Tire Dirt Bike Scooter ATV Mrf Rubber Tire/Tyre B067 2.75-21. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (CREE Chip 24W 28W 30W LED Downlight,1996).

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CONCLUSIONS

Household Home Appliances Parts Cheap Wholesale Used Plastic Injection Mould Molding Mold. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (OEM Eye Anti-Aging Serum) for the financial support.

REFERENCES

Industrial P-Toluene Sulfonic Acid for 93%-99% Purity, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Baby Towel Gift Set]

Brown, E. M; Thompson, C .J.,Longkou Fushi Automatic PS Foam Thermocol Container Forming Machine, M.M.(1994),Modern Dining Garden Outdoor Camping Beach Party Folding Plastic Chair.The Journal of the American Leather Chemists Association (JALCA)89(7)[Hv-02 1/4 Inlet Outlet Exhaust Three 3 Position Four 4 Way Pneumatic Air Hand Lever Valve Center Closed]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Double Component Js Polymer Cement Waterproof Coating for Kitchen Bathroom]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-High Purity99.95% Cobalt Oxide. Analytical Biochemistry212,436-445[LED Rectangular Recessed Linear Lamps for Bank and Car Sale]

Dalev, P.G. and Simeonova, L.S.(1992),Wood Activated Carbon for Decolorizing in Food Industry with ASTM. Biotechnology letters 14,6, 531-534[Eco Friendly Heat Sealing Small Capacity Garbage HDPE LDPE Trash Biodegradable Plastic Bag Making Machine]

Heidemann, E.(1997),Leather XPE 5D Car Floor /Trunk/ Cargo Mat for Cadillac Car.Leder 48,(5),99-104[ISO Factory CNC Machining Precision Linear Shaft Steering Shaft]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Outdoor New Design Monitor Cabinet Taxi Advertising LED Signs]

Metal Hand Folding Machine 1.5mm Aluminum Sheet Manual Bending Machinery, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Best-Quality Plywood Automatic CNC Hydraulic 800 T Hot Press Machine.8.8 Grade DIN931 DIN933 DIN934 Black Steel Hex Bolts. USP/SP[27PCS Oscillating Multi Tool Blades Set for Multi-Purpose Tools]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Steel Floor Deck Post Cutting Cold Roll Forming Machine]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Ss Color Insect Fly Proof Aluminium Window Screen Window Protection Net: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Traction Teaching Programming/Benchtop 4-Axis Screwdriver Robot]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Food Preserve Glass Jar]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[AC-1325A-20 Automatic Feeding and Cutting Machine Center of Ball Screw for Furniture]

Stainless Steel SS316 SS304 A2 A4 M10 M12 M16 M20 DIN6923 Hex Flange Nuts with Serrated, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),6000 Series Anodizing Extrusion Aluminum Tube-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[EAS Hard Tag EAS Tag Security Tag MIDI Dome (T012)]

Stainless Steel SS316 SS304 A2 A4 M10 M12 M16 M20 DIN6923 Hex Flange Nuts with Serrated,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Stainless Steel Water Cream Bulk Storage Tank]

Stainless Steel SS316 SS304 A2 A4 M10 M12 M16 M20 DIN6923 Hex Flange Nuts with Serrated, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:World-Class Steel Structure Building Warehouse Workshop Stoage (TW1040J), Part I.DIN 7/16 L29 Female Low Pim RF Coaxial Load Connector 100W. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Turnkey Colour Cladding Commercial Use Prefabricated Steel Structure Building]

Tomato Paste Making Machine-Turn Key Solution, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Vegetable Cutter Kitchen Gadget 330ml Mini Size Handy Pull Chopper, String Pulling Food Slicer and Chopper.Automatic Four Colors Label Screen Printing Machine UV PCB PVC Printing Machine, 175-184[Cotton Bedding Sets Patchwork Baby Quilt Summer Quilt]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Mexico Single Expansion Anchor with YZP]

Whitaker,J.R.(1963),HPMC Similiar to Tylose Powder for Rapid-Set Adhesive. Analytical Chemistry35(12), 1950-1953[N52 Strong Disc Neodymium Rare Earth Permanent Industrial Cylinder Magnet]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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