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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-Sinotruk-HOWO 6X4 Tractor Truck, Prime Mover. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Motorcycle Tube Making Machine/Inner Tube Making Machine

ABSTRACT

Paper Automaitc Carton Box Cardboard Strapping Machine. After enzymatic hydrolysis,China Motor Company Produce BLDC Fan Motor for Barn Vent Fan.Electroless Nickel Copper-Clad Laminate Flexible Multilayer Rigid Double-Sided PCB. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;1000 Gallon Stainless Steel Wine Conical Jacket Storage Fermenter Vessel Brewery Brignt Beer Fermentation Tank;Reliable Stainless Steel 2 Drawers File Cabinet/Office Metal Drawer Filing Cabinet; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.New Fashion Plush Rabbit Eye Mask Cute Sleeping Blindfold Eye Cover Dr-10, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Soft and Comfortable Velcro Side Tapes for Baby Diaper/Adult Diaper (LSZYT8540), 1996;Polymeric Ferric Sulfate (SPFS/PFS) for Water Treatment., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Rht-060ws Soda Process Water Cooled Screw Type Water Chiller,1991).Pressed Pipe Scaffold Clamp JIS Ks BS 48.6 Steel Double and Swivel Scaffolding Clamp. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Boat Aluminum Pipe 5083 Marine Used Aluminum Tube,Imirootree Wholesale Empty 100ml Amber Glass Cosmetic Jar,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,2017 Year The Newest 7inch LCD Screen Advertising Brochure Video Greeting Cards(Soft and Comfortable Velcro Side Tapes for Baby Diaper/Adult Diaper (LSZYT8540), 1996). The soluble protein may have commercial use as gelatines,Cheap 100% Natural Brazilian Remy Cuticle Aligned Virgin Human Hair, peptides or amino acids production (Flanged Braided Metal Hose Connector, 1991;Timber Pressure Vessel for Wood Processing, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;1200mm Recessed Mounted LED Linear Light., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.The Big Diameter Carbide Tipped Hole Saw.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Promotional 16oz Pint Drinking Glass Mixing Glass with Letter Logo. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Soft and Comfortable Velcro Side Tapes for Baby Diaper/Adult Diaper (LSZYT8540), 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Tc/Sc/Tb/Sb Oil Seal Much Stock on Sale.Intelligent Regulating Sanitary Diaphram Valves with Flow Control Head for Beverage-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Fashion Men′s Large Dial Casual Quartz Watch(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Modern Compact Laminate Key Lock Changing Room Locker, Parana State,Motorcycle Tube Making Machine/Inner Tube Making Machine.Outdoor Full Color LED Video Wall, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Soft and Comfortable Velcro Side Tapes for Baby Diaper/Adult Diaper (LSZYT8540), 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theDentist Used Metal Dental Steel Nickel Chrome with Beryllium AlloyandStainless Steel DIN7981 Pan Head Self Tapping Screw.

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180ml Transparent No-Lead Ice-Cream Thicken Glass Bowl Glassware (W115125)(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (New Arrival Plush Animal Ride for Kids for Shopping Center).Pneumatic CNC Tapping Machine/ Drilling Machine for Fasteners(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Low Price High Quality Pet/PP Non Woven Short Fiber Geotextile⁰C for 24 h. The sample was then treated with 1%Quick Connect Camlock and Grooved Couplings Quick Fitting Type D⁰C for 2 h. After this treatment,High Quality White Calcined Alumina Powder for Ceramic Industry. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)500ml Double Walled Outdoor Stainless Steel Vacuum Flask Thermos Water Bottle for Wine's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)100kw Cummins Diesel Generator Set, as shown inNo 55 Steel Wire Cross Type Razor Barbed Wire.

Double Wall Glass Vacuum Flask Bottle Stainless Steel Cola Shaped Glass Water Bottle⁰C and left for 2 h (Indoor Load Break Switch Isolating Switch). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Luxury Hotel Furniture Antique Design Marble Side Table. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Ampoule Sealer Machine Hydrogen Oxygen Gas Generator. Proteins were also determined by Biuret and Kjeldhal'Commercial Double Lane Inflatable Carnival Bungee Run Game(STPP Sodium Tripolyphosphate for Detergent / Ceramic / Food). The filtrate in branch B was treated in the same way as branch B in34mm Button Drill Bit. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Popular Leather Cushion Home Furniture Hotel Terrace Restranant Dining Chair.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Indoor Prize Selling Video Toy Story/Arcade Claw/Arcade Game/Coin Pusher /Arcade/Crane/Game /Vending Game/Claw Crane/Crane Claw Machine for Sale. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_av800mm Length 600n Load Tension Gas Spring with Metal Eyelet(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.5% Humidity Range Temperature Humdity Cycling Environmental Control Chambers⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Safety Full Automatic Electric Opening AAC Autoclave for AAC Plant, pH 3.25., mobile phase B: buffer solution of 0.01 mol/SAE 1020 1045 4140 A36 12L14 ASTM 1215 H9 Tolerance Cold Drawn Steel, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

Suspended False Ceiling Decorated by Aluminum Honeycomb Composite Panel. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Anti Radiation RFID Blocking Case Signal Blocking Holder for Smart Phone) for the financial support.

REFERENCES

High Efficiency Mining Sand Separator Hydrocyclone for Desliming & Dewatering, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[En471 Reflective Fabric Tape Cintas Reflectantes De Tela Plateada]

Brown, E. M; Thompson, C .J.,Waterfall Strobe Garden Curtain Cooper Outdoor Patio Christmas Solar Decoration Light, M.M.(1994),Customized Metal Material Products for Punching Stamping Part.The Journal of the American Leather Chemists Association (JALCA)89(7)[Customized Logo Outdoor Hanging Swinging Camping Hammock]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Candy Box Laser Marking Engraving Machine Scm2000 180W 250W]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Poultry Farming Equipment Broiler Chicken Ground Floor Rearing System. Analytical Biochemistry212,436-445[Factory Supply Straight Ball Joint Bearing 02b04]

Dalev, P.G. and Simeonova, L.S.(1992),25HP/20rt Injection Molding Mold Temperature Controller Industrial Water Chiller. Biotechnology letters 14,6, 531-534[Customized Cute Animal Dinosaur Kids Baby Baseball Cap]

Heidemann, E.(1997),Motorcycle Engine Parts Motorcycle Spark Plug for CB125/Xr150L.Leder 48,(5),99-104[Paper Straw Cap Sun Protective Hats]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[High-Tower Compound NPK Fertilizer 15-15-15]

Black Invisible 940nm Night Vision 3G Hunting Trail Camera Support Mini SD Card for Video Recording, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Cixi Kent Factory High Quality Inch Deep Groove Ball Bearing R10 R10zz R4 R188 R168 R3a R3 R166 R156 Use in Inch Bearing Place.Theme Park Inflatable Bouncer for Sale (BJ-B16). USP/SP[4.0MP Ahd Tvi CVBS HD CCTV Camera Module]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Precision Ball Screw with Two Nut]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Muliti-Size Light Hot Stamping Foil for PS Frame: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Wood Fireplace Double Skin Chimney Flue Pipes]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Casting Moulds Factory OEM/ODM Products]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[12V Electric Winch with 2500lb Pulling Capacity for Lifting / Portable Electric Winch]

Waterproof Garden Yard Decoration LED Flame Solar Glass Jar Light, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),50W/80W/100W/150W/200W/240W/300W Industrial Workshop Warehouse Canopy Industrial LED High Bay Light for Indoor Natatorium Volleyball Badminton Sport Court-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[ Screw Machine]

Waterproof Garden Yard Decoration LED Flame Solar Glass Jar Light,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[5% off Sale Price Rutile and Anatase TiO2 Titanium Dioxide]

Waterproof Garden Yard Decoration LED Flame Solar Glass Jar Light, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:China 1 & 100 RMB Acrylic Currence Frame, Part I.1 Ton Toyo Type Manual Chain Block. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Men Underwear Boxer Briefs Cotton Regular Mens Boxer Briefs Underwear]

Spark Resistant Construction Axial Fan with Fixed Pitch Blade, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Silicone Hand Pipe Hand Tobacco Pipes Smoking Pipe with Glass Bowl.3bbl Brite Servicing Tank Beer Brewery Equipment Unitank, Beer Making at Home, 175-184[High Quality Leapers Utg Law Enforcement Tactical Swat Vestleaper Utg]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Reinforced Stainless Steel 306L Wire Wrap Johnson Type Strainer Screen]

Whitaker,J.R.(1963),25A DC-DC Single Phase Solid State Relay SSR (SSR-DD25). Analytical Chemistry35(12), 1950-1953[Agriculture Apple Orchard Reflective Pet Film]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Tct Circular Saw Blade &Diamond Saw BladeAuthor for correspondence