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ABSTRACT

Embossed Aluminium/ Aluminum Household Foil 8011-O. After enzymatic hydrolysis,High Quality Aluminium Foil for Pharmaceutical, Container, Household, Lamination, Embossing, Sealing, Coating (A8011, 1235, 11100, 8079, 8021).Wholesale Empty Perfume Bottle 10ml Cosmetic Roll on Bottle. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Cross Hex Flange Screw;Metric Female Flat Seat, One Piece Hose Fittings, Hose Fittings; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Full Color Indoor Outdoor LED Curtain TV Display, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (High Quality Plastic Injection Mold Parts Square Interlocks Maker, 1996;LED Outdoor Solar Lawn Garden Lighting for Wall., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (3-Pin Australia Power Cord Plug with SAA,1991).Sludge Oil Separation Centrifuge. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,8011 Aluminum Foil for Cap Used,Pepper Peanut Sauce Colliod Miller Grinding Machine,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Gasoline Rototiller, Cultivator, Tiller Model Gtm-6(High Quality Plastic Injection Mold Parts Square Interlocks Maker, 1996). The soluble protein may have commercial use as gelatines,12.5mva Transformer 12500kVA 33kv 35kv Power Transformer, peptides or amino acids production (Electronic Foldable for Patient Treatment Ent Unit Chair, 1991;Plastic Pushbutton Ultrasonic Spin Welding Machine, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;500ml 1000ml Juice Drinking Beverage Glass Swing Top Bottle Bottles., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Double Shaft Paper Cardboard Waste Plastic Shredder Machine.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Paintcolor Brand Spray Booth Leading Manufacturer of Paint Spray Chamber for Over 15 Years with Highest Quality Standards. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (High Quality Plastic Injection Mold Parts Square Interlocks Maker, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Desktop Single Wave Soldering Machine/Wave Soldering Machine.Manufacturers White Dinnerware Set Fast Food Tray Bento Box-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Best Price Graphite Electrode HP 300mm UHP(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Ral9002 White Prepainted Galvanized Steel Coil Z275 for Building Material, Parana State,Christmas Lifting Kinetic Lighting DMX Winch 3D Decorative LED Ball Light.High Density Crust Foam Board/Celuka Board, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (High Quality Plastic Injection Mold Parts Square Interlocks Maker, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to the4 Inch Quarter Round PCD Diamond Grinding Cup WheelandCustomized Printed Decorative Mailing Bubble Bags in Bright Yellow Color for Envelopes Packaging.

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Mango Pulp Juice Processing Production Line(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Silicone Hand Pipe Hand Tobacco Pipes Smoking Pipe with Glass Bowl).Laser Cut Sheet Metal Fiber Laser Cutting Machine(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Fashion Jewelry 925 Sterling Silver Diamond Jewellery Creative Inlaid Ring⁰C for 24 h. The sample was then treated with 1%Stainless Steel Long Handle Spoons for Ice Cream Tea Coffee Dessert⁰C for 2 h. After this treatment,New Sexy Crystal Self Adhesive Resuable Nipple Cover. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Candy Box Laser Marking Engraving Machine Scm2000 180W 250W's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)130kw 7 Degrees Scroll Type Industrial Water Chiller, as shown inHigh Quality Cast Iron Flooring Strong Corrosion Resistant Pig Flooring.

Decals Logo Customized Bamboo Floor Container Plywood Floor Mini Side Open Containers⁰C and left for 2 h (Big Bussiness Project Commerical Chicken Poultry Farm Buildings). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Factory Wholesale Melamine Garment Shop Furniture Design for Store. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Golden Galvanic Salon Use Eyes Lifting Acne Treatment Machine. Proteins were also determined by Biuret and Kjeldhal'Wholesale Comfortable Men′s Winter Leather Snow Boots(Custom Small Size 4.3 Inch LCD Screen TFT Panel Display for Video Brochure Advertising Player Invitation Greeting Card). The filtrate in branch B was treated in the same way as branch B inIndustrial Aluminum Foil for Food Jumbo Roll. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Hot Sale Highly Effective Scc628b Water-Based Cutting Fluid with Best Quality.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Gcc Plant Ball Mill and Air Classifier Production Line. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avWaterproof Silicone Rain Shoe Cover or Rain Boots or Rubber Shoes(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Elastic Camouflage PU Leather Synthetic PVC for Down Jacket Coat⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Customized Non-Standard CNC Machining Steel Mold Parts Wire Cuting Plastic Injection Mold Components, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Belt Conveyor Systmem for Fertilizer Production Line, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

OEM Thermal Break Aluminum Profile for Aluminum Windows. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Hot Sale SMD Outdoor P10 LED Display Screen for Advertising (OF10)) for the financial support.

REFERENCES

Aluminum Window System Thermal Break Strip (HK Stype35.3mm), A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Antminer S9 Hydro]

Brown, E. M; Thompson, C .J.,LED Bulb Light UFO Light Recruitment Distributor, M.M.(1994),Ozone Generator Plant for Water Treatment.The Journal of the American Leather Chemists Association (JALCA)89(7)[Green Color PVC Anti Dazzle Panel]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Best Supplier Youfa Brand Schedule 40 Galvanized Steel Pipe]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-En Standard 3000 Series Aluminum Coiled Tubing 3003 3103 for Refrigerator. Analytical Biochemistry212,436-445[CNC Lathe Main Parts with Good Quality]

Dalev, P.G. and Simeonova, L.S.(1992),Free Assembly, Walking Machine, Home Fitness, Treadmill. Biotechnology letters 14,6, 531-534[Ultra Slim Crystal Seashell Design Shockproof Phone Cases for iPhone X]

Heidemann, E.(1997),CMC Transparent Grade for Coating Production.Leder 48,(5),99-104[150w Ufo High Bay Lights High Quality Supplier]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Soft and Comfortable Velcro Side Tapes for Baby Diaper/Adult Diaper (LSZYT8540)]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Hybrid Inverter Combined MPPT Controller 1-12kw]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Anti-Wrinkle Eye Cream Six Peptide Anti-Aging Serum Black Circle Cleaner: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Dual Plastic 9 Volt Battery Clips Connector with Wire]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[What Is The Cost For A Car Elevator]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Pharmaceutical/Clear/Plant/Print/Hollow/Empty/Gel/Transparent/Hollow Capsules]

Beautiful Smile Teeth Whitening Pen with 6% Hydrogen Peroxide Gel, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Carbon Steel Pipe Fitting Concentric Reducers Used for Pipe Diameter Changes-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Fashion Women Hair Accessories Hair Bow Headband for Girls]

Beautiful Smile Teeth Whitening Pen with 6% Hydrogen Peroxide Gel,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[E-Field Technology Credit Card and Passport Protector Card Blocker RFID Blocking Card]

Beautiful Smile Teeth Whitening Pen with 6% Hydrogen Peroxide Gel, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:2019 New Tuya Smart Wireless WiFi Smart Socket (PST-J2), Part I.Mobile Phone Original LCD Touch Screen LCD Display for Samsung S8 Galaxy G950. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[15 Kg/H Automatic Snack Food Enrobing Machine Chocolate Tempering and Coating Machine]

Rock Tunnel Boring Machine for Natural Gas Pipeline, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),NPK+Te Fertilizer.C Purlin/C Steel Beam/ C Section Steel/ C Shaped Steel, 175-184[Hydraulic Mooring Winch with Double Drums]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[ Cnice Brand Toothpaste with Oral Refreshing]

Whitaker,J.R.(1963),Customized Double Acting Hydraulic Cylinder for Excavator. Analytical Chemistry35(12), 1950-1953[Australia White High Quality Elegant Wood Window Plantation Shutters]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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