ARTICLES

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ABSTRACT

19*15W RGBW Beam DJ Disco Stage Light LED Moving Head Lighting. After enzymatic hydrolysis,High Quality Couple Watches Steel Material Gift Watch OEM in China.Glass Tea Sugar Coffee Storage Jar with Screw Cap. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Circular DC Electro Lifting Magnet for Lifting Scraps;Precision CNC Custom Machining Stainless Steel Aluminum Brass Hardware Parts; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Particle Board Manufacturer, Melamine/Plain Chipboard, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (CNC Milling Machinery Parts From OEM Manufacturer/ Auto Part, 1996;Villa Hotel Luxury Guest Room Decoration Standing Lamp (KA5506-5-195-160 G)., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Ttp60fras Metal Hard Alloy Tool Carbide Inserts Cutting Hand Tool Machine Tool End Tool Drill Milling Cutter Tungsten Carbide Cutter Carbide Insert,1991).Outdoor HD P5.95 Mobile LED Display Panels with Epistar Lamps. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,120ml Glass Perfume Bottles for Designer Perfume,Agriculture Hay Silage Wrapping Film,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Ce Medical Stainless Steel 6 Body Mortuary Refrigerator/Freezer(CNC Milling Machinery Parts From OEM Manufacturer/ Auto Part, 1996). The soluble protein may have commercial use as gelatines,Gc3 Lead Screw Ball Screw with Double Nuts 6000mm Ball Screw for Overhead Crane, peptides or amino acids production (16oz Insulated Cardboad Cup Sleeve, 1991;Large Outdoor Simple Gazebo Beer Festival Marquee Tent, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;HOWO Tipper Truck Price with Manufacture Factory Price., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Wood Fireplace Double Skin Chimney Flue Pipes.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-High Quality 304 Stainless Steel Sheet for Home Decoration Material. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (CNC Milling Machinery Parts From OEM Manufacturer/ Auto Part, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰T8 20W LED Grow Tube Supplementary Lighting Lamps for Plant.LED Rectangular Recessed Linear Lamps for Bank and Car Sale-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Salon Equipment Hair Clipper(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Zs Round Vibrating Sieve(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Pet Short Fiber Non Woven Geotextile for Landfill Filtration).Stainless Steel Tactile Paving Blind Road Stud(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%70L Lab Use Refrigerated Circulator Chiller Factory Price⁰C for 24 h. The sample was then treated with 1%Packaging Machine Automatic Weighing Filling Machine (AH-FJJ 100/300/500)⁰C for 2 h. After this treatment,Roller Hearth Heating Furnace for Gas Cylindrer. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)custom design MIFARE DESFire EV2 2K plastic RFID loyalty card's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Hot Saling Bathroom Furniture Solid Wood Material with Mirror by-F8016, as shown inSpp Centrifugal Chemical Electric Mixed Flow Slurry Pump.

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Dark Green Decorative Garden Welded Wire Mesh Fencing. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avCsm DIY Microscope for Kids/Toy Microscope(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.China Factory Produce Custom Logo Printed Cotton Terry Baby Neck Bib⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Hot Sale High Precision Valve Block Hydraulic Flow Control Valve 1wfl, pH 3.25., mobile phase B: buffer solution of 0.01 mol/High Speed HDMI Cable Wholesale HDMI with Ethernet, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

IS Type Cast Iron Chemical Industrial Pump. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Custom 30ml Green Dropper Glass Bottle Cosmetic Essential Oil Bottle) for the financial support.

REFERENCES

Hot Sale Automatic French Fries Production Line Fresh Potato Chips Making Machine, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Big Size Dual View Industrial X-Ray Machine]

Brown, E. M; Thompson, C .J.,Custom Grooved Quartz Plate Deep Processing, M.M.(1994),Hot Sale CNC Router Machine for Wood Cutting (1325).The Journal of the American Leather Chemists Association (JALCA)89(7)[Professional Stainless Steel Biological Fermentor]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Femo Ferro Molybdenum Powder Lump 55/60]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Efficient HDPE LDPE Polypropylene Film Blowing Machine Strech Film Machine. Analytical Biochemistry212,436-445[L Shaped Modular Kitchen Designs Kitchen Cabinet on Sale]

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Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[3-Pin Australia Power Cord Plug with SAA]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Hydraulic Electric Stationary Car Platform Scissor Elevator Lift]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-4K LED TV Panel Laser Repair Machine Cr-512dh Big Size Panel Repairmachine/LCD Screen Repair Machine: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Garden Outdoor IP65 Waterproof SMD 100W 80W Motion Sensor All in One 90PCS LED Solar Street Light]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[m.zichen-rubber.com]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[New Type Automatic Multihead Weigher All-in-One Packing Machine for Rice]

Tan Color Strip Aluminum and Glass Mosaic for Wall (M855330), M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),CNC Metal Grinding Machine Tool Mk2120-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Fire Alarm Detector 24VDC Reflective Beam Detector]

Tan Color Strip Aluminum and Glass Mosaic for Wall (M855330),M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Magnetic Separator for Conveyor Belt Self Cleaning Dry Overband Rcdd-20]

Tan Color Strip Aluminum and Glass Mosaic for Wall (M855330), M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Removable Triangular Single Slot Metal Folding Down Shelf Wall Support Bracket, Part I.Hot Sale High-Order Elasticty Girls Sports Wear Yaga Suit. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[New Technology Semi-Auto Pet Plastic Bottle Blow Molding Equipment Plant Machine]

Cast Iron Trivet for Tableware Dia: 21cm or Customize OEM, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),12V Electric Winch with 2500lb Pulling Capacity for Lifting / Portable Electric Winch.Hydraulic Electric Stationary Car Platform Scissor Elevator Lift, 175-184[Ebzt-12 Paper Cup Handle Making Machine]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Best Price Wastewater Treatment STP Treat All Kinds of Sewage to Discharge Irrigation Reuse ]

Whitaker,J.R.(1963),Australia Standard Structural Steel of Steel Structure Hangar As4100 Airport Building. Analytical Chemistry35(12), 1950-1953[2.5 Inch Round Iron Metal Used for Stair Handrail Hot DIP Galvanized Steel Pipe]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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