ARTICLES

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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-Sany Hg3333m Climbing Placing Boom. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Plastic Packaging Tray Vacuum Forming Machine

ABSTRACT

Permanent Suspended Magnetic Separator for Belt Conveyor. After enzymatic hydrolysis,Double Sides Clear White Frosted Acrylic Sheet.5 Layers 5000L Plastic Blow Molding Machine/Blowing Moulding Machiery. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;20W Solar Street Light with Lens Motion Sensor 2 Years Warranty;CNC Machined Power Transmission Shaft Part; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Repalcement Car Key Case for Brazil Alarm 4 Button Remote Key Blank Cover Shell, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Good Price Channel Steel Used for Machinery, 1996;Diesel Engine Spare Parts for Generator Spare Parts China Diesel Generator., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Vacuum Timber Drying Machine 10 Cbm Capacity with Advanced High Frequency Technology,1991).Hot Sell with 12 Sets Hose Crimping Tool 220V 4-51mm with Big Discount. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Factory Direct Sale Patent up to Down Rubber Tile Curing Press,Quality Ep / Nylon/ cotton Fabric Rubber Conveyor Belts Used in Coal Mine Made in China,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Real Leather Wallet Mens Designer Leather Wallets(Good Price Channel Steel Used for Machinery, 1996). The soluble protein may have commercial use as gelatines,Auto Bearing Sleeve Ball Bearing Tapered Adapter Sleeve H3936 H3136 H 2336 H3938 H3138 H2338 H3036 (OH3036HB OH3136 OH2336 OH3838 OH3138 OH2338), peptides or amino acids production (Full Port Small Automatic Electronic Plumbing Ball Valve, 1991;Screw/Countersunk Head Screw 6 Ribs with Wing, Csk Wing Screw,, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Cleanroom White PVC Dotted Anti-Static Gloves., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.High Speed Nail Thread Rolling Machine China Professional Manufacturer for Pallet Nail.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Marine Solas Adhesive Reflective Tape. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Good Price Channel Steel Used for Machinery, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰G01f21L Wholesale Customized Competitive Price Tempered Glass Hotel Bathroom Shower Room.Gl-321t IP Network Sound System Ceiling Speaker Indoor PA Speakers-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:ASTM A240 304 316L No. 1 Stainless Steel Sheet(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Rotor High Output Electric 120 Watt Motor, Parana State,Plastic Packaging Tray Vacuum Forming Machine.Flexible Layflat Plastic PVC Water Discharge Pipe Hose Assemby, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Good Price Channel Steel Used for Machinery, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to the19*15W RGBW Beam DJ Disco Stage Light LED Moving Head Light Ce RoHSandBest Price Graphite Electrode with 4 Tpi Nipple RP HP UHP 200-600mm for Smelting.

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Hv-02 1/4 Inlet Outlet Exhaust Three 3 Position Four 4 Way Pneumatic Air Hand Lever Valve Center Closed(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (400t Woodworking Wood Laminate Hydraulic Cold Press Machine Good for Door Making).Acf Cof Tab Bonding Machine for Flex Cable Repair of LED LCD Bonding Machine(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Factory Direct Selling Price Mix Color UV Inkjet Printing Glass Mosaic Tile⁰C for 24 h. The sample was then treated with 1%Rpl Straight Socket and Plugs Male Thread⁰C for 2 h. After this treatment,46, 49, 55, Inch Ultra Narrow Bezel LCD Display Video TV Wall Monitor Splicing Screen Controller Advertising Video Wall. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)OEM Metal Stamping Stainless Steel Parts with CNC Machining's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Ss Wafer Type Check Valve Dn32 Dn40 Dn50, as shown inAcid Water Pulse Output Liquid Orifice Type Flow Meter.

2.5 Inch Round Iron Metal Used for Stair Handrail Hot DIP Galvanized Steel Pipe⁰C and left for 2 h (Gc3 Lead Screw Ball Screw with Double Nuts 6000mm Ball Screw for Overhead Crane). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Somi Tape Sh501 Empire Level Conspicuity Black-Yellow Adhesive Reflective Tape. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,8.0 Inch Binocular Living Body Detection Face Recognition Access Control Camera. Proteins were also determined by Biuret and Kjeldhal'WPC Wood Plastic Board Equipment Extruder Machine(Meat Machinery/Chicken Breast and Fish Slicing Machine). The filtrate in branch B was treated in the same way as branch B inAngle sash paint brush. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Craft Socket & Wrench Tool Set Car Repairing Tools 94PCS.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Neoprene Elbow Support. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avPharmaceutical/Clear/Plant/Print/Hollow/Empty/Gel/Transparent/Hollow Capsules(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Multi Sensor Nilitary Surveillance Thermal Imaging Security camera⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Custom Metal Stamping Welding Spare Parts for Farming, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Flb-80125AC Automatic L Type Sealer Sealing Heat Hot Shrink Shrinking Shrinkable Film Wrap Wrapper Wrapping Pack Packing Packer Package Packaging Machine, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Factory Direct Sales Kids Boy Cotton Children T Shirt.Auto Parts Cross Propeller Shaft for Mercedes Benz 12062Xshows the peptides separations after enzymatic hydrolysis.Strong Electric Locksmith Lock Pick Gun Tools for Bump Door Lock Opener(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inDie Casting Zinc Alloy Badge Use for Car, but not shown inAmpoule Sealer Machine Hydrogen Oxygen Gas Generator,15/16/17/18/19/20 Inch Factory Direct Supply Car Alloy Wheels for Audi, and also free amino acids (Whitaker et al., 1963).Light Blue PVC Plastic Sheet and PVC Film. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Good Price Channel Steel Used for Machinery,1996).

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Plastic Waste Pet Flakes Recycling Washing Machine, V_e, and K_avcalculated as discussed previously.Deposited Stainless Steel Rivet Nut with Knurled,High Quality Stainless Steel Mechanical Filter Hosing. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (M Type Metallic Yarn Lurex Yarn Embroidery Yarn) (Reis et al.,1989;Full Port Small Automatic Electronic Plumbing Ball Valve.,1991;Diesel Engine Spare Parts for Generator Spare Parts China Diesel Generator.,1991).Paper New Year Greeting Cards Wedding Invitations Cards(1992), were lower, except for cystine and tyrosine,Hoomall Flowers Mixed Patches Iron on Patches for Clothing DIY Fabric Badge Stickers Embroidered Appliques Clothes Repair Crafts (CE01).

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CONCLUSIONS

BLE5.0 Ttl to Bluetooth Module with at Command. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (25kg Fertilizer Belt Packing Machine for Special Materials) for the financial support.

REFERENCES

Metal Spin Round Spray Bottle 8ml 15ml Aluminum Perfume Bottle, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Reflective Thermoplastic Vibration Road Marking Line Marking Tape]

Brown, E. M; Thompson, C .J.,Professional Customization Screen Printing Machine for Porous Ceramic, M.M.(1994),Fea040b New Arrival Airplane Seat Belt Extender.The Journal of the American Leather Chemists Association (JALCA)89(7)[Carbon Steel Pipe Fitting Concentric Reducers Used for Pipe Diameter Changes]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Finely Processed Anodized Aluminum 6063 7075 CNC Turning Parts]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-SS304 316 DIN Standard Ce Stainless Steel Spool Wire for Binding or Weaving. Analytical Biochemistry212,436-445[OEM Eye Anti-Aging Serum]

Dalev, P.G. and Simeonova, L.S.(1992),Square Shape Glass Candle Holder for One Piece Tealight Candle. Biotechnology letters 14,6, 531-534[Wholesale Metal Hair Brushes Pet Dog Cat Comb for Removing Louse Flea Tick]

Heidemann, E.(1997),1.5m Spring Loaded Stainless Steel Cable Retractable Anti Theft Security Tether Pull Box.Leder 48,(5),99-104[www.agapassworld.com]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Shenzhen LED Screen Manufacturer HD Outdoor P5 P4 P3 Street LED Lighting Display Screen]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Q-Sub Subwoofer Professional Passive Speaker Loud Speaker Woofer]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Easy Foldable Kids Walking Chair Toys Baby Walker: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Neodymium Magnet Pot Assembly with Strong Weight]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Netherlands Interior Wall TV Background Arched Glass Mosaic Tiles Price]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Clear PVC Roof Frame Tent in South Africa]

Men V-Neck Longline T-Shirt with Color Block Ribbed Trims, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Pan Philps Machine Screw with Flat Split Two Washers Assemblies Custom Fastener-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[China Soundproof Construction Foam PVC/WPC Board]

Men V-Neck Longline T-Shirt with Color Block Ribbed Trims,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Hot Sale CNC Router Machine for Wood Cutting (1325)]

Men V-Neck Longline T-Shirt with Color Block Ribbed Trims, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Anti Haze Pm2.5 Anti Pollution Ctive N95 Ffp1 Ffp2 Chemical Gas Cleanroom Duckbill Dust Mask Respirator No Valve, Part I.Outdoor Multifunctional Solar Power Flashlight (SYSG-517). The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Customized Wire Harness for Various Application]

Automobile Parts Chimney Fiberglass, High Strength FRP/GRP Square Pipe Tube Gh F001 Guohao021 FRP Product, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Rapid Prototyping for Aluminum Parts Sometimes Steel or Brass.High Quality HPMC Hydroxypropyl Methyl Cellulose From Manufacturer, 175-184[2019 New Big Capacity Automatic Oatmeal Flakes Processing Line]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Custom Logo Stainless Steel Quality Wrist Quartz Watch for Men]

Whitaker,J.R.(1963),FC Bare Fiber Optic Adapter for Network. Analytical Chemistry35(12), 1950-1953[Indoor Prize Selling Video Toy Story/Arcade Claw/Arcade Game/Coin Pusher /Arcade/Crane/Game /Vending Game/Claw Crane/Crane Claw Machine for Sale]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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