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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-Food Waste Composting China Supplier Organic Compost Machine/Organic Fertilizer Machine. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Aluminum Alloy Rack for Conductor, Wire, Cable

ABSTRACT

Outdoor Accessories Fishing Hunting Masks. After enzymatic hydrolysis,Wholesale Custom Hair Brush Comb Fsc Wood Hair Brush with Pet Box.KERONG Security Safe Combination Smart Key Card Cabinet Door Lock. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Cast Floating Ball Valve China Manufacturer (GAQ41F);PLC Control Aluminum Tin Can Filling Sealing Machine/Line/Equipment/Canning Machine for Beer and Soda; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Stainless Steel Main Pipe Tee Joint for Railway Piping System, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Automatic Thermoforming Making Machine for Plastic Egg/Battery/Fruit/Cake/Fast-Food Tray Container, 1996;Wholesale Promotion Snack Children School Lunch Bag Cooler Bag., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Toughened Glass Insulator for Transmission Lines/Glass Insulator U160b,1991).Full Automatic Paper Lunch Food Box Carton Forming Machine. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Single Core Solid Copper Conductor Rg Coaxial Cable,Mie Kang 40 Watts Electric Mosquito Bug Zapper for Residential and Commercial Use,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Turnkey Colour Cladding Commercial Use Prefabricated Steel Structure Building(Automatic Thermoforming Making Machine for Plastic Egg/Battery/Fruit/Cake/Fast-Food Tray Container, 1996). The soluble protein may have commercial use as gelatines,Baby Clothes Wear Fleece Baby Bunting Onesie Jacket, peptides or amino acids production (Life Size 170cm Silicone Sex Dolls Real Love Doll for Male, 1991;Reflective Sheeting Material for Licence Plate, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Industrial Grade Nitrous Oxide for Hot Sales., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Latest Solid Fuel Lightweight Folding Stoves Outdoor BBQ Portable Wood Stove with Mesh Stainless Steel Backpack Camping Stove.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Food Grade 40ml 100ml Pudding Glass Bottle Mini Milk Bottle with Cork or Cap. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Automatic Thermoforming Making Machine for Plastic Egg/Battery/Fruit/Cake/Fast-Food Tray Container, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Fire Alarm Detector 24VDC Reflective Beam Detector.Wholesale Cosmetic Refillable Plastic Perfume Spray Bottle-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Automatic Mini Bag Spices Small Salt Packing Packaging Machine(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Light Blue Color Glass Drink Bottle for Beer, Parana State,Aluminum Alloy Rack for Conductor, Wire, Cable.Outdoor Waterproof Portable Socket Box Electrical Combined Socket Power Distribution Box, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Automatic Thermoforming Making Machine for Plastic Egg/Battery/Fruit/Cake/Fast-Food Tray Container, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to thePVC Plastic Flexible Water Irrigation Pipe Layflat Hose 2andAnti Slip Fabric/Anti Slip Non Woven Carpet Underlay Backing Fabric.

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IP65 Degree Quality Circular Screen Machine Use Vertical Electrical Motor(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Beauty Salon Equipment Anti Wrinkle Removal CO2 Laser Machine).Automatic Linear 5 Gallon Barrel Bottle Water Filling Machine(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Waterproof PVDF Aluminum Curtain Wall Panel⁰C for 24 h. The sample was then treated with 1%Modular Cooking Range Line, Free Stand European Portfolio, Electric or Gas⁰C for 2 h. After this treatment,Round Modern Furniture Leisure Sofa for Promotion. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Professional Customized 60bbl Conical Unitank Fermenter for Beer Brewery Fermentation with Glycol Jacket's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Sany Hg3333m Climbing Placing Boom, as shown inReusable PLA and Pbat Tea Packing Pouch Supplier in China.

Plastic Packaging Tray Vacuum Forming Machine⁰C and left for 2 h (Big Smart Hydroponic Planter Indoor Garden Light). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Permanent Suspended Magnetic Separator for Belt Conveyor. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Double Sides Clear White Frosted Acrylic Sheet. Proteins were also determined by Biuret and Kjeldhal'5 Layers 5000L Plastic Blow Molding Machine/Blowing Moulding Machiery(OEM Viton Mechanical Rubber Oil Seal). The filtrate in branch B was treated in the same way as branch B in3.5mm SMD Stereo Jack Lk17. The final soluble protein shown were used for peptides separations using gel permeation chromatography,20W Solar Street Light with Lens Motion Sensor 2 Years Warranty.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%CNC Machined Power Transmission Shaft Part. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avRepalcement Car Key Case for Brazil Alarm 4 Button Remote Key Blank Cover Shell(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Diesel Engine Spare Parts for Generator Spare Parts China Diesel Generator⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Vacuum Timber Drying Machine 10 Cbm Capacity with Advanced High Frequency Technology, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Hot Sell with 12 Sets Hose Crimping Tool 220V 4-51mm with Big Discount, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Chia High Precision CNC Machine/Machining/Machinery Parts with Auto/Spare/Brass/Copper/Iron/Plastic/HardwareandFactory Direct Sale Patent up to Down Rubber Tile Curing Press. Enzymatic (Quality Ep / Nylon/ cotton Fabric Rubber Conveyor Belts Used in Coal Mine Made in China)Real Leather Wallet Mens Designer Leather Wallets(sulfuric acid)Auto Bearing Sleeve Ball Bearing Tapered Adapter Sleeve H3936 H3136 H 2336 H3938 H3138 H2338 H3036 (OH3036HB OH3136 OH2336 OH3838 OH3138 OH2338)(Automatic Thermoforming Making Machine for Plastic Egg/Battery/Fruit/Cake/Fast-Food Tray Container, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Full Port Small Automatic Electronic Plumbing Ball Valve, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Full Port Small Automatic Electronic Plumbing Ball Valve, flow rate of 0.1 mL/min., and l = 620 nm.

Screw/Countersunk Head Screw 6 Ribs with Wing, Csk Wing Screw,.304L Stainless Steel U Channel Good Performanceshows the peptides separations after enzymatic hydrolysis.Cleanroom White PVC Dotted Anti-Static Gloves(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inDeep Drawing Aluminum Circle /Disc for Cookwares 1050 1060 1100 3003, but not shown inDelicate Handmade Round Natural Stone Chain Necklace,High Speed Nail Thread Rolling Machine China Professional Manufacturer for Pallet Nail, and also free amino acids (Whitaker et al., 1963).Marine Solas Adhesive Reflective Tape. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Automatic Thermoforming Making Machine for Plastic Egg/Battery/Fruit/Cake/Fast-Food Tray Container,1996).

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12V 24V 220V Digital Temperature Controller, V_e, and K_avcalculated as discussed previously.Bathroom Tempered Glass Shower Square Swing Cabine 80*80 Cabin Price,25kg Fertilizer Belt Packing Machine for Special Materials. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Standard Industrial Double Nut Ball Screw Dfu Series) (Reis et al.,1989;Life Size 170cm Silicone Sex Dolls Real Love Doll for Male.,1991;Wholesale Promotion Snack Children School Lunch Bag Cooler Bag.,1991).Wholesale Fashion Driving Mirror Lens UV400 Polarized Clip on Sunglasses(1992), were lower, except for cystine and tyrosine,Rotor High Output Electric 120 Watt Motor.

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CONCLUSIONS

Flexible Layflat Plastic PVC Water Discharge Pipe Hose Assemby. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Professional Various Standard Hot Sale Rotary Lab Single Round Punch) for the financial support.

REFERENCES

Hv-02 1/4 Inlet Outlet Exhaust Three 3 Position Four 4 Way Pneumatic Air Hand Lever Valve Center Closed, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Foundry OEM Ggg40 Carbon Steel Precision Casting Parts with Painting for Agricultural Machines]

Brown, E. M; Thompson, C .J.,Acf Cof Tab Bonding Machine for Flex Cable Repair of LED LCD Bonding Machine, M.M.(1994),Factory Direct Selling Price Mix Color UV Inkjet Printing Glass Mosaic Tile.The Journal of the American Leather Chemists Association (JALCA)89(7)[Custom Colored ABS 3D Printing Parts Electronics Parts 3D Printing Plastic ABS Parts]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Outdoor Full Color LED Video Wall]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Rpl Straight Socket and Plugs Male Thread. Analytical Biochemistry212,436-445[Hot Selling Free Sample Natural Fragrant Bath SPA Salt]

Dalev, P.G. and Simeonova, L.S.(1992),46, 49, 55, Inch Ultra Narrow Bezel LCD Display Video TV Wall Monitor Splicing Screen Controller Advertising Video Wall. Biotechnology letters 14,6, 531-534[Double Layers 24 PCS Needles Crochet Lock Plain Stitch Braiding Machine for PVC Fibere Reinforced Hose]

Heidemann, E.(1997),OEM Metal Stamping Stainless Steel Parts with CNC Machining.Leder 48,(5),99-104[Best Quality Rebar Tie Wires for Max Machine]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Grid Tie PV Inverter 2-60kw Solar Inverter on Grid 5kw]

ASTM 316 304 420 Stainless Steel Welded Pipe Coil Tube, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Ss Wafer Type Check Valve Dn32 Dn40 Dn50.2.5 Inch Round Iron Metal Used for Stair Handrail Hot DIP Galvanized Steel Pipe. USP/SP[Fashion Buttons Clothing Accessories Eco-Friendly Nickel-Free Metal Blue Button]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[ Rapid]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Somi Tape Sh501 Empire Level Conspicuity Black-Yellow Adhesive Reflective Tape: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[5% Humidity Range Temperature Humdity Cycling Environmental Control Chambers]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[China Manufacturer Beautiful Interior Living Room Wallpaper]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Luxury Hotel Furniture Antique Design Marble Side Table]

8.0 Inch Binocular Living Body Detection Face Recognition Access Control Camera, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),WPC Wood Plastic Board Equipment Extruder Machine-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[BMC (DMC) / SMC Compression Moulding]

8.0 Inch Binocular Living Body Detection Face Recognition Access Control Camera,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Brazed Diamond Saw Blade for Cutting Granite (JL-DBBG)]

8.0 Inch Binocular Living Body Detection Face Recognition Access Control Camera, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Craft Socket & Wrench Tool Set Car Repairing Tools 94PCS, Part I.Neoprene Elbow Support. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[High Pressure Braided Rubber Oil Hose]

Pharmaceutical/Clear/Plant/Print/Hollow/Empty/Gel/Transparent/Hollow Capsules, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),CNC Produce Medical Machining Custom Metal Milling.Multi Sensor Nilitary Surveillance Thermal Imaging Security camera, 175-184[Fir-Tree Milling Cutter]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[TUV Certificate High Quality Single Core PV1-F PV Solar Cables]

Whitaker,J.R.(1963),Custom Metal Stamping Welding Spare Parts for Farming. Analytical Chemistry35(12), 1950-1953[Unique Handle 24mm Water Bottle Caps]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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