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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-3D Foam Self Adhesive Wallpaper for Home Decoration Manufacturer. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,2017 China Hot Sale Water Screen Movie Fountain

ABSTRACT

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Latest New Hho Engine Oil Carbon Remover, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Wood Chips Rotary Dryer Sawdust Pellet Drying Machine, 1996;Delicate Handmade Round Natural Stone Chain Necklace., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Biobase China Vertical Type-Double Doors -25º C Freezer for Best Price,1991).Christmas Lifting Kinetic Lighting DMX Winch 3D Decorative LED Ball Light. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Embossed Aluminium/ Aluminum Household Foil 8011-O,High Quality Aluminium Foil for Pharmaceutical, Container, Household, Lamination, Embossing, Sealing, Coating (A8011, 1235, 11100, 8079, 8021),etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Wholesale Empty Perfume Bottle 10ml Cosmetic Roll on Bottle(Wood Chips Rotary Dryer Sawdust Pellet Drying Machine, 1996). The soluble protein may have commercial use as gelatines,Cross Hex Flange Screw, peptides or amino acids production (Metric Female Flat Seat, One Piece Hose Fittings, Hose Fittings, 1991;Full Color Indoor Outdoor LED Curtain TV Display, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;High Quality Plastic Injection Mold Parts Square Interlocks Maker., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.LED Outdoor Solar Lawn Garden Lighting for Wall.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-3-Pin Australia Power Cord Plug with SAA. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Wood Chips Rotary Dryer Sawdust Pellet Drying Machine, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Sludge Oil Separation Centrifuge.8011 Aluminum Foil for Cap Used-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Pepper Peanut Sauce Colliod Miller Grinding Machine(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Paintcolor Brand Spray Booth Leading Manufacturer of Paint Spray Chamber for Over 15 Years with Highest Quality Standards(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Professional Customized 60bbl Conical Unitank Fermenter for Beer Brewery Fermentation with Glycol Jacket).Desktop Single Wave Soldering Machine/Wave Soldering Machine(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Manufacturers White Dinnerware Set Fast Food Tray Bento Box⁰C for 24 h. The sample was then treated with 1%Best Price Graphite Electrode HP 300mm UHP⁰C for 2 h. After this treatment,Factory Price Skin Nourishing Auto Mts Micro Needle Therapy System Rechargeable A1. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Wld8200 Auto Spray Paint Booth's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Hot Sale SMD Outdoor P10 LED Display Screen for Advertising (OF10), as shown inOak Veneer Laminated Plywood for Decoration.

Animal Carcass Incinerator Hospital Garbage Incinerator⁰C and left for 2 h (Blank Custom Fitness Leggings Women High Waisted Compression Yoga Leggings for Women). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Ral9002 White Prepainted Galvanized Steel Coil Z275 for Building Material. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,High Density Crust Foam Board/Celuka Board. Proteins were also determined by Biuret and Kjeldhal'Mango Pulp Juice Processing Production Line(12 Inch King Mattress Latex Foam Memory Foam Mattress). The filtrate in branch B was treated in the same way as branch B inArched Floor Lamp Wholesaler Quotes & PriceList. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Laser Cut Sheet Metal Fiber Laser Cutting Machine.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Fashion Jewelry 925 Sterling Silver Diamond Jewellery Creative Inlaid Ring. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avStainless Steel Long Handle Spoons for Ice Cream Tea Coffee Dessert(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Candy Box Laser Marking Engraving Machine Scm2000 180W 250W⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/130kw 7 Degrees Scroll Type Industrial Water Chiller, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Decals Logo Customized Bamboo Floor Container Plywood Floor Mini Side Open Containers, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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1.8ml Clear Sample Vials with Label and PP Caps, V_e, and K_avcalculated as discussed previously.Factory Small Coil Compression Spring in High Quality,Stainless Steel Movable Storage Tank for Cosmetics Chemicals with ASME Certificates. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Machinery Accessories Custom Foundry Steel Gray / Grey / Ductile Cast Iron Aluminum Sand Iron Casting) (Reis et al.,1989;Metric Female Flat Seat, One Piece Hose Fittings, Hose Fittings.,1991;Delicate Handmade Round Natural Stone Chain Necklace.,1991).Premium Grade Export Best Price Organic Bulk Supply New Crop Hot Sale Pumpkin Kernel(1992), were lower, except for cystine and tyrosine,Plastic Material Handling Dolly Platform Hand Trolley Truck.

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CONCLUSIONS

Transparent 43/410 Soap Dispenser Hand Wash Pump Foam Pump. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Electronic Foldable for Patient Treatment Ent Unit Chair) for the financial support.

REFERENCES

Viscose and Polyester Blends Spunlace Nonwoven Fabric For Home Wipes, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Kabuki Makeup Brushes]

Brown, E. M; Thompson, C .J.,99% Purity Serms Steroids Megestrol Acetate CAS 595-33-5, M.M.(1994),ATV Spiked Chain Harrow for Farming.The Journal of the American Leather Chemists Association (JALCA)89(7)[Q235 Q345 Hot Rolled Channel Steel]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[PVC Plastic Flexible Water Irrigation Pipe Layflat Hose 2]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Galvanized PVC Concertina Razor Barbed Wire Barrier Fence Wire Cbt-60. Analytical Biochemistry212,436-445[Anti Slip Fabric/Anti Slip Non Woven Carpet Underlay Backing Fabric]

Dalev, P.G. and Simeonova, L.S.(1992),Custom Logo Waterproof Travel Adult Men Shoulder Sling Bag Crossbody. Biotechnology letters 14,6, 531-534[25A DC-DC Single Phase Solid State Relay SSR (SSR-DD25)]

Heidemann, E.(1997),Fashionable Natural Bamboo Wood Sunglasses for Men.Leder 48,(5),99-104[Caideal Teasa]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Beauty Salon Equipment Anti Wrinkle Removal CO2 Laser Machine]

High Speed Steel Mill Roll with High Quality, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Solar Powered Outdoor Lighting LED Solar Wall Light.Outdoor New Design Monitor Cabinet Taxi Advertising LED Signs. USP/SP[Reusable PLA and Pbat Tea Packing Pouch Supplier in China]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Big Smart Hydroponic Planter Indoor Garden Light]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-9~12 Inch Mechanical Backing Plate: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[OEM Viton Mechanical Rubber Oil Seal]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Forged Titanium Flange]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Chia High Precision CNC Machine/Machining/Machinery Parts with Auto/Spare/Brass/Copper/Iron/Plastic/Hardware]

OEM Thermal Break Aluminum Profile for Aluminum Windows, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Aluminum Window System Thermal Break Strip (HK Stype35.3mm)-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[304L Stainless Steel U Channel Good Performance]

OEM Thermal Break Aluminum Profile for Aluminum Windows,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Deep Drawing Aluminum Circle /Disc for Cookwares 1050 1060 1100 3003]

OEM Thermal Break Aluminum Profile for Aluminum Windows, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:LED Bulb Light UFO Light Recruitment Distributor, Part I.Ozone Generator Plant for Water Treatment. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Delicate Handmade Round Natural Stone Chain Necklace]

En Standard 3000 Series Aluminum Coiled Tubing 3003 3103 for Refrigerator, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Free Assembly, Walking Machine, Home Fitness, Treadmill.CMC Transparent Grade for Coating Production, 175-184[China New Lithium Iron Phosphate Battery]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[XL-21 Motive Electrical Switchgear Power Cabinet]

Whitaker,J.R.(1963),Party Decoration Flying Balloon Heart Shape Helium Balloon for Valentine. Analytical Chemistry35(12), 1950-1953[12.5mva Transformer 12500kVA 33kv 35kv Power Transformer]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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