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ABSTRACT

Microfiber Towel Car Drying Soft Towel. After enzymatic hydrolysis,Factory Competitive Prices 1-4 Core G657A2 Self Supported Drop Fiber Optic Cable FTTH.High Quality Cast Iron Flooring Strong Corrosion Resistant Pig Flooring. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.New Styles Fashion Erotic Women Underwear Sexy Lingerie, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (High Quality 1530 Metal Cutting Cheap CNC Plasma Pipe Cutting Machine with Rotary, 1996;Custom Precision Sheet Metal Forming Bending Welding Parts Housing Stamping., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (200 Tons Press Brake 4000mm CNC Press Brake 200 Tons Hydraulic Press Brake with Bending 8mm,1991).Fully Automatic Water Bottle Lids Labeling Machine. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,2u Lgx Swing out Fiber Optic Patch Panel with 4 SC Simplex Single Mode Adapter Panels,New CCTV 4.0MP H. 265+ IR Bullet Waterproof IP Camera,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Non-Woven Anti-Static Briefs...Non-Woven Anti Static Briefs(High Quality 1530 Metal Cutting Cheap CNC Plasma Pipe Cutting Machine with Rotary, 1996). The soluble protein may have commercial use as gelatines,Fused Silica Quartz Lump 5-50mm for Refractory Materials, peptides or amino acids production (Cold Drawn D2 H13 Alloy Steel Turning Roll, 1991;Interlocking Anti-Fatigue Kitchen Rubber Mat, Kitchen Floor Mat, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Bread Box Bamboo Large Capacity Roll Top Bread Holder Cupcake Storage Food Bin for Loaf Pastry Snacks., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.High Quality Suitcase Frame or Profile Bending Machine with Ce Aprroved.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Colorful 100LED Copper String Light Outdoor LED. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (High Quality 1530 Metal Cutting Cheap CNC Plasma Pipe Cutting Machine with Rotary, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰H365 Emas Chainsaw Spares Cylinder Kits.PP Woven Bag for Feed, Rice, Corn Feed Bag Rice Bag 10, 25, 50 Kg-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:A4 Diploma Covers Wholesale with Silver Foil(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Dental YAG Laser Welding Machine for Gold Silver Metal Jewelry⁰C and left for 2 h (Hot Saling Bathroom Furniture Solid Wood Material with Mirror by-F8016). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Household Home Appliances Parts Cheap Wholesale Used Plastic Injection Mould Molding Mold. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Industrial P-Toluene Sulfonic Acid for 93%-99% Purity. Proteins were also determined by Biuret and Kjeldhal'Longkou Fushi Automatic PS Foam Thermocol Container Forming Machine(Metal Cuting CNC Plasma Cutting Machine (DW1325)). The filtrate in branch B was treated in the same way as branch B inSmall Carry Trolley Suitcase Bag. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Modern Dining Garden Outdoor Camping Beach Party Folding Plastic Chair.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%High Purity99.95% Cobalt Oxide. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avWood Activated Carbon for Decolorizing in Food Industry with ASTM(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Best-Quality Plywood Automatic CNC Hydraulic 800 T Hot Press Machine⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/8.8 Grade DIN931 DIN933 DIN934 Black Steel Hex Bolts, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Ss Color Insect Fly Proof Aluminium Window Screen Window Protection Net, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

Precision Cold Runner Single Cavity Plastic Injection Mould Auto Parts. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (120L 240L Oil Universal Chemical Spil Absorbent Kits) for the financial support.

REFERENCES

Household Family Medical Emergency Electronic Digital Thermometer, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Rubber Artificial Pussy]

Brown, E. M; Thompson, C .J.,Die Cast Iron Worm Gear Speed Reduce Gearbox, M.M.(1994),19*15W RGBW Beam DJ Disco Stage Light LED Moving Head Lighting.The Journal of the American Leather Chemists Association (JALCA)89(7)[New Styles Fashion Erotic Women Underwear Sexy Lingerie]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[1.0mm Binding Wire Galvanized Iron Wire]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-High Quality Couple Watches Steel Material Gift Watch OEM in China. Analytical Biochemistry212,436-445[Bathroom Tempered Glass Shower Square Swing Cabine 80*80 Cabin Price]

Dalev, P.G. and Simeonova, L.S.(1992),Glass Tea Sugar Coffee Storage Jar with Screw Cap. Biotechnology letters 14,6, 531-534[115mm Segmented Saw Blade for Concrete Cutting]

Heidemann, E.(1997),Circular DC Electro Lifting Magnet for Lifting Scraps.Leder 48,(5),99-104[Shopping Cart For Elderly]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[OEM Factory New Girls Nuts Crunch Cheap Price]

OEM Eye Anti-Aging Serum, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Precision CNC Custom Machining Stainless Steel Aluminum Brass Hardware Parts.Particle Board Manufacturer, Melamine/Plain Chipboard. USP/SP[Fluorous Rubber Coated Fiberglass Cloth]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Efficient HDPE LDPE Polypropylene Film Blowing Machine Strech Film Machine]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-CNC Milling Machinery Parts From OEM Manufacturer/ Auto Part: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Building Wall Machine for Hot Machine]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Glass Double Layer Water 320ml Bottle with Stainless Steel Lids]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Best Price Graphite Electrode HP 300mm UHP]

Villa Hotel Luxury Guest Room Decoration Standing Lamp (KA5506-5-195-160 G), M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Ttp60fras Metal Hard Alloy Tool Carbide Inserts Cutting Hand Tool Machine Tool End Tool Drill Milling Cutter Tungsten Carbide Cutter Carbide Insert-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Through Bolt Assy for Montabert Brh-501 Hydraulic Breaker Hammers]

Villa Hotel Luxury Guest Room Decoration Standing Lamp (KA5506-5-195-160 G),M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Desktop Single Wave Soldering Machine/Wave Soldering Machine]

Villa Hotel Luxury Guest Room Decoration Standing Lamp (KA5506-5-195-160 G), M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Outdoor HD P5.95 Mobile LED Display Panels with Epistar Lamps, Part I.120ml Glass Perfume Bottles for Designer Perfume. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Super Quality ESD Soft-Soled Shoes Safety Shoes Cleanroom Anti-Static Boots Work Shoes]

Agriculture Hay Silage Wrapping Film, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Ce Medical Stainless Steel 6 Body Mortuary Refrigerator/Freezer.Gc3 Lead Screw Ball Screw with Double Nuts 6000mm Ball Screw for Overhead Crane, 175-184[3 Panels Canvas Screen]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Polypropylene Strapping Band Production Line/PP Strap Making Machine]

Whitaker,J.R.(1963),16oz Insulated Cardboad Cup Sleeve. Analytical Chemistry35(12), 1950-1953[Double Wall Glass Vacuum Flask Bottle Stainless Steel Cola Shaped Glass Water Bottle]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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