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ABSTRACT

Ningbo Smart Professional Manufacturer Rx Brass Pneumatic Pipe Fitting. After enzymatic hydrolysis,Metal Animal Longhorn Skull Pendant Longhorn Skull Charm.Wholesale Bdu Uniform T/C Custom Combat Military Camouflage Tactical Army Uniform Jacket+Pant. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Eco Friendly Heat Sealing Small Capacity Garbage HDPE LDPE Trash Biodegradable Plastic Bag Making Machine;High Output Plastic Rubber Filing Masterbatch Extruder Machine; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.High Precision Mold Manufacturer Plastic Injection Mould, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (China Wholesale Oxide Aluminium Black Sand Blast Media, 1996;Welded Stud Anchor Chain for Ship., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (SGCC Cold Rolled Z180 Coating Galvanized Metal Steel Strip for Industry,1991).Line Scan Uvss System Portable Under Vehicle Surveillance. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Folding Steel Manual Wheelchair with Chrome Frame,Piston Cooling Nozzle Valve Body Made of 45# 35# Carbon Steel,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Fashion Style Lady Alloy Quartz Watch with Simple Dial(China Wholesale Oxide Aluminium Black Sand Blast Media, 1996). The soluble protein may have commercial use as gelatines,Rooftop HVAC Packaged Units (RTU) Amrta Manufacture, peptides or amino acids production (Waterproof IP67 Outdoor Plastic Box Enclosure Electronic Junction Box, 1991;Building Decoration Material Acrylic Dispersant for Coating, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;High Speed Plastic PE Single Wall Corrugated Hose Pipes Extruder Making Machine., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Diamond Tools Continous Saw Blade Segment Bldes Turbo Saw Blades Brazed Welded Saw Blades.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Factory Supply Straight Ball Joint Bearing 02b04. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (China Wholesale Oxide Aluminium Black Sand Blast Media, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Wrinkle Effect Crease UV Drying Machine UV Curing System.Lithium Polymer 3000mAh 634169-3s 11.1V High Quality Li-ion Battery Pack-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Best Pinpointer Metal Detector Hand Held Underground Gold Metal Detector(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

20 40 Stainless Steel High Pressure Cartridge Filter Housing China FactoryAlcalase2.5 L DX,Tefude Feeding and Packaging Line for Food with Tray, with activity of 2.5 (Anson unity) and optimal activity at pH 8.5-9.5 (Nova Nordisk S/A-Aluminum Alloy Front Control Panel Machining Monitoring Linear Combination Parts); Biuret reagent (Bioclin-Quibasa); standard solution of protein mixture IV (12300-78000 Daltons-Merck); standard solution of 0.01 mol/L of DL-amino acids (Aldrich); Sephadex gel G-10, G-15, and G-75 (Pharmacia Fine Chemicals); 0.4% (m/v) solution of blue dextran (Sigma); 0.2% (m/v) solution of ninhydrin (Sigma) dissolved in acetone; 0.02%(m/v)Automatic Sawdust/Rice Husk/Biomass Pellet Making Machine(Aldrich); 0.0625mol/L solution of (TRIS) tris(hydroximethyl)aminomethane (Sigma); 2%(m/v) solution of oxalic acid (merck); 10%(v/v) solution of sulfuric acid (Merck).

Sample collection and preparation

CMP No Nc Metal Switch Black 25mm Illuminated Push Button, Parana State,New Type Automatic Multihead Weigher All-in-One Packing Machine for Rice.Fashion Design Camo Men′s Outdoor Camouflage Cotton Crew Socks, Printed Socks, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (China Wholesale Oxide Aluminium Black Sand Blast Media, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theRemy Human Hair Straight 1b#613 Full Lace/Lace Front Wig on SaleandManufacturers White Dinnerware Set Fast Food Tray Bento Box.

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Full Hard S550gd Cold Rolled Galvanzied Steel Sheet in Coil(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Ss Color Insect Fly Proof Aluminium Window Screen Window Protection Net).Felt Disc Polishing Sanding Disc with Wool Felt Diam(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%13.56MHz Contactless RFID Card MIFARE 1K Card for Prepaid Payment System⁰C for 24 h. The sample was then treated with 1%Thermomolded TPE 5X20mm IP68 10K Air Conditioner Probe Black Cable⁰C for 2 h. After this treatment,100% Fiberglass 1*1m Fire Blanket 3732 Fiberglass Cloth. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)240T goodeng water/oil/gas pipe drilling rig HDD machine's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Mens Messenger Bag 15.6 Inch Waterproof Waxed Canvas, as shown inDecals Logo Customized Bamboo Floor Container Plywood Floor Mini Side Open Containers.

Colored Silicone NBR O-Rings Seals, Rubber O Ring with High Strength⁰C and left for 2 h (180ml Transparent No-Lead Ice-Cream Thicken Glass Bowl Glassware (W115125)). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Reinforced Stainless Steel 306L Wire Wrap Johnson Type Strainer Screen. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Colorful Braided Fabric Cable Round. Proteins were also determined by Biuret and Kjeldhal'Universal Wholesale Hot Sale Custom Blank Keys(High Quality Disposable Blood Transfusion Set with Filter). The filtrate in branch B was treated in the same way as branch B inBonded Knit Fabric. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Latex Free Olive Face Cut Makeup Sponge /Oval Makeup Sponge Not Makeup Remover Sponge.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Rotary Automatic Wet Wipe Production Linewet Wipes Machine RM-Wl30. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avWaw 8 Ton Logistics Light Truck for Transportation(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Hot Sale Chic Straw Braid Floppy Hat with Print Tape⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/High Quality Plastic Butterfly Valve UPVC DIN ANSI JIS Standard Wafer Worm Gear Butterfly Valve PVC Level Butterfly Valve for Water Supply, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Lamella Clarifier in Sewage Tretament Plant for Water Purification, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Custom FSTN Va COB Cog Segment LCD Display ModuleandBMC (DMC) / SMC Compression Moulding. Enzymatic (Ring Caps &Plastic Tube Pop Novelties)Wholesale Fashion Handbags Clutch Coin Purse White Ladies PU Wallet(sulfuric acid)Aiyos New Floor Standing 55 Inch Narrow Bezel Touch Screen Advertising Interactive Kiosk(China Wholesale Oxide Aluminium Black Sand Blast Media, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Dual Plastic 9 Volt Battery Clips Connector with Wire, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Dual Plastic 9 Volt Battery Clips Connector with Wire, flow rate of 0.1 mL/min., and l = 620 nm.

Precision Ball Screw with Two Nut.Dresser Coupling Ductile Iron Dismantling Joint Flexible Compensatorshows the peptides separations after enzymatic hydrolysis.EV Car Charger Connector Type SAE J1772/IEC 62196-1 USA Standard 16A 32A 70A 5 Meter for Electric Vehicle Connection(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inFull Automatic 100X120mm Hexagonal Gabion Woven Wire Mesh Knitting Machine, but not shown inAutomatic Floor Cylinder Shot Blasting Machine Road Cleaning Abrator,Low Density Cleaner in Paper Making Machine, and also free amino acids (Whitaker et al., 1963).En471 Reflective Fabric Tape Cintas Reflectantes De Tela Plateada. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (China Wholesale Oxide Aluminium Black Sand Blast Media,1996).

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Swf Coreline Set Tc Core Bit for Drilling Softer Unconsolidated FormationsandFood Grade Beer/Juice Treatment Bag Mamachine SUS304 316L Stainless Steel Bag Filter Vessel with Ss Removable Frame Holdershow the gel chromatographic separation of soluble protein with Sephadex G-Customized Stainless Steel Laser Cutting.Price of a Retail Supermarket Push Cart for Sale, and another peak at 45 mL not shown inLED UV Curing Machine for UV Cured Floor Coatings TM-LED600-6, for peptides lower than 3000 Da.Indoor UTP Cat 6 with Rj 45 Connector Length 20 Meter Cable(Condell et al.,1993) .

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OEM Stainless Steel Stamping Parts Custom Stainless Steel Sheet Fabrication Metal Stamping Fabricate, V_e, and K_avcalculated as discussed previously.Handmade Natural Bamboo Basket / Bamboo Flower Pot,Stainless Steel Handrail Bar Holder for 16mm Pipe. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Top Furniture Hotel Dining Furniture Metal Hotel Table Chair) (Reis et al.,1989;Waterproof IP67 Outdoor Plastic Box Enclosure Electronic Junction Box.,1991;Welded Stud Anchor Chain for Ship.,1991).Shac Flange Linear Bearing(1992), were lower, except for cystine and tyrosine,China Supplier Hot Sale Electric Hydraulic Vertical Freight Lift with Cheap Price.

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CONCLUSIONS

Heat Resistant Adhesive Teflon Tape Price High Temperature Teflon Tape. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Aluminum Alloy Front Control Panel Machining Monitoring Linear Combination Parts) for the financial support.

REFERENCES

CNC Router for Engraving Cutting Carving Drilling Milling, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Aluminum Sheet Metal Stamping]

Brown, E. M; Thompson, C .J.,Electric Manual Ice Crusher Ice Shaver Industrial Ice Machine, M.M.(1994),Horizontal Rustproof Multi-Function Blood Storage Refrigerator Yj-40-60-Wa.The Journal of the American Leather Chemists Association (JALCA)89(7)[Black White Stone 925 Silver Cross Pendants Necklace Jewelry]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[CNC Machined Power Transmission Shaft Part]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Empty 20ml Pocket Perfume Spray Bottles 20 Ml PP Plastic Card Shape Bottle with Sprayer. Analytical Biochemistry212,436-445[Waterproof Silicone Rain Shoe Cover or Rain Boots or Rubber Shoes]

Dalev, P.G. and Simeonova, L.S.(1992),High Quality Hot Rolled Steel U Channel China Supplier. Biotechnology letters 14,6, 531-534[Plastic Water Gas Supply Cable Protect PE LDPE HDPE Pipe Extrusion Machine Line Single Screw Extruder]

Heidemann, E.(1997),M Type Metallic Yarn Lurex Yarn Embroidery Yarn.Leder 48,(5),99-104[Washed White Aluminum Oxide]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[CREE Chip 24W 28W 30W LED Downlight]

Sheet Metal Distribution Cabinet with High Quality, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Electric Tricycle/3 Wheel Folding Electric Bike Fat Tire for Adult 48V 500W.Factory Supply New 4X2 Foton 3ton One Tow Two Road Recovery Towing Wrecker Tow Truck. USP/SP[FC Bare Fiber Optic Adapter for Network]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Nellcor SpO2 Extension Sensor Cable 5 Core SpO2 Adapter Cable]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-500W 1000W Stainless Steel Copper Fiber Metal Cutting Equipment: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Customized Non-Standard CNC Machining Steel Mold Parts Wire Cuting Plastic Injection Mold Components]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[10 Rolls Plastic Shopping Bag Making Machine for T-Shirt Bag]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Stainless Steel Handrail Bar Holder for 16mm Pipe]

YCOM High Quality Displayport to HDMI Cable Dp to HDMI Cable, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Anti-Pollution Modified Acrylic Solid Surface for Countertops-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[6063 Series Aluminium Extrusion Pipe in Hot Sell Made in China]

YCOM High Quality Displayport to HDMI Cable Dp to HDMI Cable,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[PVC Powder Coated Galvanized Metal Welded Wire Mesh Fence]

YCOM High Quality Displayport to HDMI Cable Dp to HDMI Cable, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Double Layers 24 PCS Needles Crochet Lock Plain Stitch Braiding Machine for PVC Fibere Reinforced Hose, Part I.Corrugated Carton Box Fully Automatic Folding Gluing Machine. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Wholesale Price Decorative Wrought Iron Stairs Aluminium Metal Garden Fence]

800lb 1/2 A105 Thread Forged Steel Globe Valve, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Korea Hot Sale 24K Goldzan Ampoule 99.9% Pure Gold Face Essence.China Manufacturer Cheap Custom Running Award Metal Sport Trophie Medal, 175-184[Small Type 4lz-4.0e Wheat Cutter Mini Harvester Price]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Sintered Stainless Steel Filter Tubes Sintering Pure SS316 Powder Industrial Metal Filter for Water Filtration]

Whitaker,J.R.(1963),CNC Machining Metal Mechanical Engineering Parts. Analytical Chemistry35(12), 1950-1953[Outdoor Multifunctional Solar Power Flashlight (SYSG-517)]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Cross Hex Flange ScrewAuthor for correspondence