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ABSTRACT

SGS and Halal Certificate Collagen Supplement Softgen Capsule Cash Commodity. After enzymatic hydrolysis,High Quality Rubber Pressed Mixing Machine with Ce Certification.Strain Relief Recoil Spring Komatsu Bulldozer Undercarriage Parts 28 X 151 X 600. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Medical Ce Approved Fast Hair Removal IPL Shr Beauty Machine for Sale (HS-310), about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Mj3210 Vertical Wood Cutting Band Saw Machine with Log Carriage for Log Planks/Lumber Cutting, 1996;Wholesale Wax Dry Herb Tank Container Glass Jar Cbd Thc Tin Can., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Practical Edible Silicone Kitchenware (YB-ty-29),1991).4 Inch Quarter Round PCD Diamond Grinding Cup Wheel. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,High-Speed Automatic Tri-Sealing Zipper Vertical Bag Making Machine,Brazed Diamond Saw Blade for Cutting Granite (JL-DBBG),etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Hot Sale Smoking Accessories Silicone Smoking Pipes Tobacco Hand Pipes with Glass Bowl(Mj3210 Vertical Wood Cutting Band Saw Machine with Log Carriage for Log Planks/Lumber Cutting, 1996). The soluble protein may have commercial use as gelatines,99% Hatching Rate Newest Design 48 Eggs Chicken Incubator (YZ8-48), peptides or amino acids production (Spp Centrifugal Chemical Electric Mixed Flow Slurry Pump, 1991;China Cube Ice Maker Parts / Ice Machines Maker Cube, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Aluminium/Aluminum Casement Window with Break Bridge (FT-W108)., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.3D Adjustable American Market Cabinet Door Mini Hinge.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Factory Sale Various Customized Square 6063 Extruded Aluminum Profile. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Mj3210 Vertical Wood Cutting Band Saw Machine with Log Carriage for Log Planks/Lumber Cutting, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Custom Design 100% Silk Tie for Men.Portable Camping Parachute Nylon Double Green Hammock Tent with Mosquito Netting-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Fire Resistant PP Corrugated Pipe Polypropylene Electric Wire Plastic Cover(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Hot Selling Knitting Velvet Jaguar Fabric (JAGUAR)(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (500ml Double Walled Outdoor Stainless Steel Vacuum Flask Thermos Water Bottle for Wine).Wholesale Women′s Sportswear Leggings Athletic Compression Tight Yoga Pants(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Seasoned Cast Iron South Africa Three Legged Potjie Pot⁰C for 24 h. The sample was then treated with 1%1.0g Water and Air Ozone Generator with Silicone Tube⁰C for 2 h. After this treatment,Multifunctional Two Cryopencils RF 40K Cavitation Vacuum Body Slimming Machine. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)16A/24A High Speed Wire Cable Braiding Machine's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Traction Teaching Programming/Benchtop 4-Axis Screwdriver Robot, as shown inSmall Table CNC Marble Engraver for Stone.

5 Axis CNC Machine with German Technology (BL-Y850/1050 VMC850/1050)⁰C and left for 2 h (Brown /Black /Red Film Faced Plywood Shuttering Poplar Core Waterproof). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.20 Inch 48V 750W Fat Tire Electric Bike with Double Seat. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Agriculture Apple Orchard Reflective Pet Film. Proteins were also determined by Biuret and Kjeldhal'High Precision IP54 Waterproof Digital Vernier Caliper 6 Electronic Caliper (WW-54DC04)(Ad-L13 Black Perfume Plastics Vial Bottle 10ml). The filtrate in branch B was treated in the same way as branch B inANSI Hex Nylon Insert Self Lock Nuts. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Small Boat Deck Knuckle Boom Ship Pedestal Crane.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%115mm Segmented Saw Blade for Concrete Cutting. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avAutomatic Vertical Packaging Machine Filling Machine for Chips, Popcorn, Macaroni, Hamburgers, Oats, Washing Powder, Puffed Food, Biscuit(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.3D Creative Design Metal Medal Promotion Gift of Sport⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Ad-L13 Black Perfume Plastics Vial Bottle 10ml, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Patchwork Color Outdoor Covers Stretch Waterproof Auto Car Cover, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Best Supplier Youfa Brand Schedule 40 Galvanized Steel Pipe.Plastic Mining Biaxial Geogrid Similar to Tensar Mining Gridshows the peptides separations after enzymatic hydrolysis.SSAW Steel Pipe API 5L Psl2 with Stainless Steel Sheet(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared in3 Years Warranty 3.2V 60ah 75ah 86ah 100ah 110ah 200ah Single LiFePO4 Battery Cell LiFePO4 Lithium Iron Phosphate Battery Cell for EV, but not shown inPlastic Material Handling Dolly Platform Hand Trolley Truck,Ribbon Production of Various Specifications of Gold and Silver Wire Rope Kraft Paper Rope Hand Rope, and also free amino acids (Whitaker et al., 1963).Whole Absorbtion Flexo Printing Soltting Die Cutting Machine. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Mj3210 Vertical Wood Cutting Band Saw Machine with Log Carriage for Log Planks/Lumber Cutting,1996).

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Air Purification Activated Alumina, V_e, and K_avcalculated as discussed previously.Steel Tubeless Truck Trailer Rim Wheel (17.5X6.75 17.5X6),Resistan UV Grades PP Non Woven Fabric. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (OEM Stainless Steel Stamping Parts Custom Stainless Steel Sheet Fabrication Metal Stamping Fabricate) (Reis et al.,1989;Spp Centrifugal Chemical Electric Mixed Flow Slurry Pump.,1991;Wholesale Wax Dry Herb Tank Container Glass Jar Cbd Thc Tin Can.,1991).New Scba Breathing Apparatus Equipment with Ce Certification(1992), were lower, except for cystine and tyrosine,FRP Pultruded Pipe or GRP Profile.

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CONCLUSIONS

6090 CO2 Sealed Glass Cooling Laser Cutting Machine. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Canned Fruit Peach Processing Plant) for the financial support.

REFERENCES

Aluminum Alloy Die Casting Parts LED Lamp Housing Radiator, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Headlight Checking Fixture]

Brown, E. M; Thompson, C .J.,Sexy Women Dress Invisible Zipper Jump Suits Fashion Hidden Zipper, M.M.(1994),Outdoor Fitness Equipment Swing (JMH-03).The Journal of the American Leather Chemists Association (JALCA)89(7)[Cheap 100% Natural Brazilian Remy Cuticle Aligned Virgin Human Hair]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[ etc Underground Metel Detector]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Sheet Metal Distribution Cabinet with High Quality. Analytical Biochemistry212,436-445[Latest Solid Fuel Lightweight Folding Stoves Outdoor BBQ Portable Wood Stove with Mesh Stainless Steel Backpack Camping Stove]

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Heidemann, E.(1997),New Type Automatic Multihead Weigher All-in-One Packing Machine for Rice.Leder 48,(5),99-104[Wheel Hub Bearings]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Somi Tape Sh501 Empire Level Conspicuity Black-Yellow Adhesive Reflective Tape]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Building Material of Granite Ceramic Wall Tile for Wall Decoration]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Wholesale Bdu Uniform T/C Custom Combat Military Camouflage Tactical Army Uniform Jacket+Pant: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Plastic Flange Housing Ucf310-115 for Electric Hand Tools]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Transistor 2sc3047]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Rock Tunnel Boring Machine for Natural Gas Pipeline]

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Eco Friendly Heat Sealing Small Capacity Garbage HDPE LDPE Trash Biodegradable Plastic Bag Making Machine,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[DIN1478 Closed Body Jaw Jaw Wire Rope Turnbuckle]

Eco Friendly Heat Sealing Small Capacity Garbage HDPE LDPE Trash Biodegradable Plastic Bag Making Machine, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:High Precision Mold Manufacturer Plastic Injection Mould, Part I.China Wholesale Oxide Aluminium Black Sand Blast Media. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Embossed Aluminium/ Aluminum Household Foil 8011-O]

Welded Stud Anchor Chain for Ship, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),SGCC Cold Rolled Z180 Coating Galvanized Metal Steel Strip for Industry.Line Scan Uvss System Portable Under Vehicle Surveillance, 175-184[Fashion Jewelry 925 Sterling Silver Diamond Jewellery Ring with Butterfly]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Lamella Clarifier in Sewage Tretament Plant for Water Purification]

Whitaker,J.R.(1963),Folding Steel Manual Wheelchair with Chrome Frame. Analytical Chemistry35(12), 1950-1953[Interlocking Precast Plastic Concrete Paver Molds for Sale]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Epimedium/Horny Goat Weed Extract/C Yin Yang Huo Extract/Barrenwort Extract Lcariin/Epimedium Extract PowderAuthor for correspondence