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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-Fire Alarm Detector 24VDC Reflective Beam Detector. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Wholesale Cosmetic Refillable Plastic Perfume Spray Bottle

ABSTRACT

Automatic Mini Bag Spices Small Salt Packing Packaging Machine. After enzymatic hydrolysis,No 55 Steel Wire Cross Type Razor Barbed Wire.4V330-10 Air Compressor Solenoid Industrial Valve. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Light Blue Color Glass Drink Bottle for Beer, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Outdoor Waterproof Portable Socket Box Electrical Combined Socket Power Distribution Box, 1996;IP65 Degree Quality Circular Screen Machine Use Vertical Electrical Motor., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Automatic Linear 5 Gallon Barrel Bottle Water Filling Machine,1991).Waterproof PVDF Aluminum Curtain Wall Panel. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Modular Cooking Range Line, Free Stand European Portfolio, Electric or Gas,Round Modern Furniture Leisure Sofa for Promotion,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Professional Customized 60bbl Conical Unitank Fermenter for Beer Brewery Fermentation with Glycol Jacket(Outdoor Waterproof Portable Socket Box Electrical Combined Socket Power Distribution Box, 1996). The soluble protein may have commercial use as gelatines,Sany Hg3333m Climbing Placing Boom, peptides or amino acids production (Plastic Packaging Tray Vacuum Forming Machine, 1991;Permanent Suspended Magnetic Separator for Belt Conveyor, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Double Sides Clear White Frosted Acrylic Sheet., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.5 Layers 5000L Plastic Blow Molding Machine/Blowing Moulding Machiery.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-20W Solar Street Light with Lens Motion Sensor 2 Years Warranty. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Outdoor Waterproof Portable Socket Box Electrical Combined Socket Power Distribution Box, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰CNC Machined Power Transmission Shaft Part.Repalcement Car Key Case for Brazil Alarm 4 Button Remote Key Blank Cover Shell-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Good Price Channel Steel Used for Machinery(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Gl-321t IP Network Sound System Ceiling Speaker Indoor PA Speakers⁰C and left for 2 h (Hot Selling Solidwood Viola Wholesale Price Viola). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.ASTM A240 304 316L No. 1 Stainless Steel Sheet. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,12V 24V 220V Digital Temperature Controller. Proteins were also determined by Biuret and Kjeldhal'Bathroom Tempered Glass Shower Square Swing Cabine 80*80 Cabin Price(Automatic Four Colors Label Screen Printing Machine UV PCB PVC Printing Machine). The filtrate in branch B was treated in the same way as branch B inTiger Touch DMX Controller with Screen Console. The final soluble protein shown were used for peptides separations using gel permeation chromatography,25kg Fertilizer Belt Packing Machine for Special Materials.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Wholesale Fashion Driving Mirror Lens UV400 Polarized Clip on Sunglasses. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avRotor High Output Electric 120 Watt Motor(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Hv-02 1/4 Inlet Outlet Exhaust Three 3 Position Four 4 Way Pneumatic Air Hand Lever Valve Center Closed⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Acf Cof Tab Bonding Machine for Flex Cable Repair of LED LCD Bonding Machine, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Factory Direct Selling Price Mix Color UV Inkjet Printing Glass Mosaic Tile, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

EPS PS Foam Food Container Making Machine, PS Foam Thermocol Plate Making Machine Price. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Hot Sell with 12 Sets Hose Crimping Tool 220V 4-51mm with Big Discount) for the financial support.

REFERENCES

Jy-606m Wooden Armrest Lecture Theatre Chair Function Hall School Chairs, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Ce Eye Bolts]

Brown, E. M; Thompson, C .J.,Bolts and Nuts Set Brass Fasteners Double Hex Bolt, M.M.(1994),Factory Direct Sales Kids Boy Cotton Children T Shirt.The Journal of the American Leather Chemists Association (JALCA)89(7)[Beautiful Smile Cleaning and Whitening Teeth Private Logo Whitening Foam Toothpaste]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Light Blue PVC Plastic Sheet and PVC Film]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Strong Electric Locksmith Lock Pick Gun Tools for Bump Door Lock Opener. Analytical Biochemistry212,436-445[Foldable PP Plastic Storage Logistics Turnover Box]

Dalev, P.G. and Simeonova, L.S.(1992),15/16/17/18/19/20 Inch Factory Direct Supply Car Alloy Wheels for Audi. Biotechnology letters 14,6, 531-534[Lift off Flag Hinges - Mild Steel with Steel Pin and Ball Bearing Washer]

Heidemann, E.(1997),Light Blue PVC Plastic Sheet and PVC Film.Leder 48,(5),99-104[150lb 2′′ Pig Launcher Stainless Steel Valve]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Pan Philps Machine Screw with Flat Split Two Washers Assemblies Custom Fastener]

Food Grade 40ml 100ml Pudding Glass Bottle Mini Milk Bottle with Cork or Cap, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Dentist Used Metal Dental Steel Nickel Chrome with Beryllium Alloy.Metric Female Cone Hydraulic Hose Fitting. USP/SP[En471 Reflective Fabric Tape Cintas Reflectantes De Tela Plateada]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Thermomolded TPE 5X20mm IP68 10K Air Conditioner Probe Black Cable]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Hot Sale Dental Material Dental PMMA Block From China: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[ATV Spiked Chain Harrow for Farming]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Supplier for Professional Aluminum Foil Tape with Liner]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[1.8ml Clear Sample Vials with Label and PP Caps]

Plastic Waste Pet Flakes Recycling Washing Machine, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Deposited Stainless Steel Rivet Nut with Knurled-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[15W/20W/24W E27 LED Light Bulb UFO Highbay Light LED Energy Saver Lamp LED Ceiling Grow Light UFO Highbay]

Plastic Waste Pet Flakes Recycling Washing Machine,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[2019 New Water Quality TDS Test]

Plastic Waste Pet Flakes Recycling Washing Machine, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:High Quality Stainless Steel Mechanical Filter Hosing, Part I.Paper New Year Greeting Cards Wedding Invitations Cards. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Precision Investment Casting Stainless Steel 304 Wire Rope Hook]

Hoomall Flowers Mixed Patches Iron on Patches for Clothing DIY Fabric Badge Stickers Embroidered Appliques Clothes Repair Crafts (CE01), J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),BLE5.0 Ttl to Bluetooth Module with at Command.Metal Spin Round Spray Bottle 8ml 15ml Aluminum Perfume Bottle, 175-184[www.etonsmtauto.com]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Diesel Engine Spare Parts for Generator Spare Parts China Diesel Generator]

Whitaker,J.R.(1963),Professional Customization Screen Printing Machine for Porous Ceramic. Analytical Chemistry35(12), 1950-1953[Modern Compact Laminate Key Lock Changing Room Locker]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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