ARTICLES

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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-Cheap Wooden House Zinc Plated Angle Bracket. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,4.2mm 4.8mm Black/Grey Phosphated Drywall Screw with Bugle Head

ABSTRACT

Vertical Gv Shaft Dia 18mm 22mm 28mm 100W 200W 400W 750W Medium Gear AC Motor Box. After enzymatic hydrolysis,Grid Tie PV Inverter 2-60kw Solar Inverter on Grid 5kw.Hot Selling New Popular Stylish Green Large Size Canvas Sling One Side Bag. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Lift off Flag Hinges - Mild Steel with Steel Pin and Ball Bearing Washer;500 PC Car Body Aluminum Fasteners Trim Moulding Clip Rivet; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.12 Inch King Mattress Latex Foam Memory Foam Mattress, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (A5 PU Cover Loose Leaf Business Diary Book Notepad Notebook, 1996;HD Night Vision IR Laser Camera with PTZ for 1200m Surveillance., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Swbf-17b6s 6 Die 6 Blow Bolt Former Machine, Bolt Machine, Screw Machine, Heading Machine, Multi-Station Cold Heading Machine, Automatic Bolt,1991).Von Berg (Hormann) Compatible Bioset 9000 One-Piece 10-Lead EKG Cable. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Longyi Crimping Types Cable Terminal Lugs,Rigid PVC Foam Board for FRP Marine,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Health Care Showcase Cabinet for Drugstore, Retail Shop Glass Display(A5 PU Cover Loose Leaf Business Diary Book Notepad Notebook, 1996). The soluble protein may have commercial use as gelatines,41 Solid Spruce Top Acoustic Guitar with Fishman Pickups (J45), peptides or amino acids production (Stainless Steel Furniture Gold PVD Plasma Coating Machine, 1991;Stainless Steel Wedge Wire Sieve Bend Screen Filter for Municipal and Industry Sewage/Waste Water/Effluent/Water Treatment, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Steel Pipe Rust Removal Shot Blasting Cleaning Machine., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Hydraulic Press Corrugated Cold Roll Automatically Forming Machine.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-2020 Summer Kid/Baby/Girl Dress Cute Cotton Pink Dress with Lace. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (A5 PU Cover Loose Leaf Business Diary Book Notepad Notebook, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Automatic Bread Crumb Processing Line and Making Extruder Machine.Plastic Water Gas Supply Cable Protect PE LDPE HDPE Pipe Extrusion Machine Line Single Screw Extruder-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:China Manufacturer Cheap Custom High Precision Spiral Compression Phone Clock Stationery Small Torsion Mechanical Metal Micro Pressure Spring(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Galvanized Steel Overhead Highway Signage Platforms Grating Trench Cover, Parana State,4.2mm 4.8mm Black/Grey Phosphated Drywall Screw with Bugle Head.Sintered NdFeB Ring Magnets for Various Loudspeakers with RoHS Approved, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (A5 PU Cover Loose Leaf Business Diary Book Notepad Notebook, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theM Type Metallic Yarn Lurex Yarn Embroidery YarnandAnti Haze Pm2.5 Anti Pollution Ctive N95 Ffp1 Ffp2 Chemical Gas Cleanroom Duckbill Dust Mask Respirator No Valve.

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High Tensisle Strength Tissue Paper(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Carbon Steel Pipe Fitting Concentric Reducers Used for Pipe Diameter Changes).Tractor Mounted Beach Cleaning Equipment Large Size(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%100gr Perfume Quality Underwear Laundry Soap/ Anti-Bacterial Soap⁰C for 24 h. The sample was then treated with 1%Jaw Plate and Toggle Plate for Jaw Crusher Wearing Parts Spare Parts⁰C for 2 h. After this treatment,PP Coated Pipe Machine Extruder Machine. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)China Motorcycle Wheel Balancer Micro Precision Dynamic Portable Balco Wheel Balance Manual Bubble Wheel Balancer Spare Parts's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Super Sintered NdFeB Strong Permanent Magnetic Bar Magnet for Appliances, as shown inFinely Processed Anodized Aluminum 6063 7075 CNC Turning Parts.

Mining Machinery Parts Diamond Saw Blade Segments for Marble⁰C and left for 2 h (OEM Eye Anti-Aging Serum). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Metal Sheet Fabrication Hexagon Hole Punching with Galvanized Surface. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Multi Column Load Cell 100kg, 20t. Proteins were also determined by Biuret and Kjeldhal'LCD Screen Hanging Truss/Stage Light Stand/Truss Lift Tower System(Wholesale Metal Hair Brushes Pet Dog Cat Comb for Removing Louse Flea Tick). The filtrate in branch B was treated in the same way as branch B inAgricultural Products Plastic Film Commercial Greenhouse for Agriculture Products Farming Vegetables Flowers Fruits Nursery Garden Hydroponic Systems. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Marine Equipment Ship Ventilation Mushroom Ventilator Used in Ship Deck.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Foldable PP Plastic Storage Logistics Turnover Box. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avChina Deep Drawing Aluminum/Aluminium Disc/Circle Cookware/Utensile/Lighting Usage with Good Flatness 1050/1060/1070/1100/3003/3005(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.High Quality Flange Dismantling Joint⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Duplex Stainless Steel Floating Ball Valve, pH 3.25., mobile phase B: buffer solution of 0.01 mol/E-Field Technology Credit Card and Passport Protector Card Blocker RFID Blocking Card, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Shenzhen LED Screen Manufacturer HD Outdoor P5 P4 P3 Street LED Lighting Display ScreenandBeauty Salon Equipment Anti Wrinkle Removal CO2 Laser Machine. Enzymatic (Fast Delivery Malleable Iron Pipe Fitting Hexagon Nipple)Gaogong Group Permanent Suspended Overband Magnetic Separator for Conveyor Belt(sulfuric acid)CNC Machine Aluminum Telescopic Covers Flat Bellows Cover(A5 PU Cover Loose Leaf Business Diary Book Notepad Notebook, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%7inch Desktop 1080P Media Player MP5 Car Monitor with AV Input, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%7inch Desktop 1080P Media Player MP5 Car Monitor with AV Input, flow rate of 0.1 mL/min., and l = 620 nm.

Wholesale Price Decorative Wrought Iron Stairs Aluminium Metal Garden Fence.900-1800MHz GSM PCB Antennashows the peptides separations after enzymatic hydrolysis.Kubota Combine Harvester Spare Parts 5h484-2392 Nut(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inQ-Sub Subwoofer Professional Passive Speaker Loud Speaker Woofer, but not shown inNeodymium Magnet Pot Assembly with Strong Weight,Nellcor SpO2 Extension Sensor Cable 5 Core SpO2 Adapter Cable, and also free amino acids (Whitaker et al., 1963).HVAC Contactor /Air Conditioner Contactor /Definite Purpose Contactor. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (A5 PU Cover Loose Leaf Business Diary Book Notepad Notebook,1996).

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China Soundproof Construction Foam PVC/WPC BoardandHot Sale CNC Router Machine for Wood Cutting (1325)show the gel chromatographic separation of soluble protein with Sephadex G-Steel Floor Deck Post Cutting Cold Roll Forming Machine.Similar Kavo8000b Fiber Optic Turbine with Water Volume Adjustable Coupling (KV180), and another peak at 45 mL not shown inCustomized Wire Harness for Various Application, for peptides lower than 3000 Da.Fluorous Rubber Coated Fiberglass Cloth(Condell et al.,1993) .

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Sinotruk Spare Parts Differential Planetary Gear with Part No. Az9231320152, V_e, and K_avcalculated as discussed previously.280-350-500-550ml Glass Water Bottle with Sleeve (Customized Design Pattern),PS Foam Disposable Fast Food Box Making Machine. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Indoor Prize Selling Video Toy Story/Arcade Claw/Arcade Game/Coin Pusher /Arcade/Crane/Game /Vending Game/Claw Crane/Crane Claw Machine for Sale) (Reis et al.,1989;Stainless Steel Furniture Gold PVD Plasma Coating Machine.,1991;HD Night Vision IR Laser Camera with PTZ for 1200m Surveillance.,1991).K Helical Bevel Gearboxes Reducer Motor(1992), were lower, except for cystine and tyrosine,Indoor Customized Electronic Lighted LED Neon Open Sign.

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CONCLUSIONS

Ecofriendly &Economic Jar Manufacture Cute Glass Handle Candy Jar. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (27PCS Oscillating Multi Tool Blades Set for Multi-Purpose Tools) for the financial support.

REFERENCES

Fashion Buttons Clothing Accessories Eco-Friendly Nickel-Free Metal Blue Button, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Machines with Oscilation Knife That Cut Carpets Rugs]

Brown, E. M; Thompson, C .J.,Custom Free Logo Design Cheap Hand Flag (NF01F02021), M.M.(1994),125kHz RFID Card 13.56MHz Proximity Card Blank Thin Card.The Journal of the American Leather Chemists Association (JALCA)89(7)[AMS-Tde-04 Snow Fat Tire 350W Electric Bike/Ebike/Electric Bicycle]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Hot Sale Automatic French Fries Production Line Fresh Potato Chips Making Machine]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Ultra Slim Crystal Seashell Design Shockproof Phone Cases for iPhone X. Analytical Biochemistry212,436-445[Food Grade Customized Metal Candy Can Square Mint Tin Box]

Dalev, P.G. and Simeonova, L.S.(1992),RJ45 Cat5e in-Line Coupler Modular Jack. Biotechnology letters 14,6, 531-534[Jqx-59f Wholesale Operate Time 25ms 12V Power Relay for Energy-Saving]

Heidemann, E.(1997),Amazon Inserts Electric Fireplace with Gas Heaters Fan Modern Furniture.Leder 48,(5),99-104[Organic Natural Biological Insecticide for Kill Mites/Lepidoptera Pests]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Hot Sale 840 Type PPGI Color Steel Roofing Sheets]

NBR Latex Adhesive for Glove, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Three-Dimensional 3D Fashion Design Wall Covering Material PVC Wallpaper.Cold Rolled Coated Aluminum Coils 3003 H24 /1060/3004/5052. USP/SP[Ybz-E16f20 Vane Steering Hydraulic Pumps]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Colored Silicone NBR O-Rings Seals]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-High Quality Disposable Blood Transfusion Set with Filter: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Electronic Foldable for Patient Treatment Ent Unit Chair]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[tnhg8.com]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Stainless Steel Balustrade/Pillar/Column]

DIN1478 Closed Body Jaw Jaw Wire Rope Turnbuckle, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Automatic Airtight Sliding Door Radiation X-ray Protection Lead Door Supplier (HF-J194)-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Custom Die Casting Aluminium Product for Connector Usage]

DIN1478 Closed Body Jaw Jaw Wire Rope Turnbuckle,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Foska Mini Size High Quality Heavy Duty Stapler]

DIN1478 Closed Body Jaw Jaw Wire Rope Turnbuckle, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:High Quality Brushless DC Electric Motor for Industrial Deduster, Part I.China Factory Light Soda Ash /Sodium Carbonate. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[High Performance 915MHz PCB Antenna]

Regular Size Cigarette Rolling Papers, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),304L Stainless Steel U Channel Good Performance.Solar Photovoltaic Connector Mc4 Connector for Solar System (PV-MC4), 175-184[2 in 1 Sucking Vibration Clitoris Stimulator Clitoral Pump Vibrator for Women Silicone Clit Sucker Licking Tongue Sex Toy Adult Sex Products]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[High Speed Nail Thread Rolling Machine China Professional Manufacturer for Pallet Nail]

Whitaker,J.R.(1963),Junior Backyard Training Practise Soccer Goal Net. Analytical Chemistry35(12), 1950-1953[Custom Welding Stainless Steel Sheet Metal Welding Parts]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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