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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-IS Type Cast Iron Chemical Industrial Pump. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Hot Sale Automatic French Fries Production Line Fresh Potato Chips Making Machine

ABSTRACT

Custom Grooved Quartz Plate Deep Processing. After enzymatic hydrolysis,Hot Sale CNC Router Machine for Wood Cutting (1325).Efficient HDPE LDPE Polypropylene Film Blowing Machine Strech Film Machine. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Mini Succulent Planters, Cactus Pots with Wooden Cement Pot, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Paper Egg Box Forming Molding Production Line/ Egg Tray Machine, 1996;4K LED TV Panel Laser Repair Machine Cr-512dh Big Size Panel Repairmachine/LCD Screen Repair Machine., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Tan Color Strip Aluminum and Glass Mosaic for Wall (M855330),1991).CNC Metal Grinding Machine Tool Mk2120. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Removable Triangular Single Slot Metal Folding Down Shelf Wall Support Bracket,Hot Sale High-Order Elasticty Girls Sports Wear Yaga Suit,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Cast Iron Trivet for Tableware Dia: 21cm or Customize OEM(Paper Egg Box Forming Molding Production Line/ Egg Tray Machine, 1996). The soluble protein may have commercial use as gelatines,12V Electric Winch with 2500lb Pulling Capacity for Lifting / Portable Electric Winch, peptides or amino acids production (Hydraulic Electric Stationary Car Platform Scissor Elevator Lift, 1991;Australia Standard Structural Steel of Steel Structure Hangar As4100 Airport Building, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Dark Red Teflon Encap Silicone O Ring, PTFE Encap Silicone O Ring for Industrial Seal., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Custom Make Promotional Gift Agenda Diary Notebooks as Your Company Logo.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-N52 Strong Disc Neodymium Rare Earth Permanent Industrial Cylinder Magnet. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Paper Egg Box Forming Molding Production Line/ Egg Tray Machine, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Bulk Packing 20kg Double Plastic Bags Outer Carton Peanut Butter (Peanut Paste).Remy Human Hair Straight 1b#613 Full Lace/Lace Front Wig on Sale-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:High Quaity China Supplier Truck Trailer Auto Car Accessories(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Natural Cleaning Konjak Sponge, Parana State,Hot Sale Automatic French Fries Production Line Fresh Potato Chips Making Machine.Indoor Load Break Switch Isolating Switch, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Paper Egg Box Forming Molding Production Line/ Egg Tray Machine, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to the3bbl Brite Servicing Tank Beer Brewery Equipment Unitankand130kw 7 Degrees Scroll Type Industrial Water Chiller.

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%A5 PU Cover Loose Leaf Business Diary Book Notepad Notebook. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avHD Night Vision IR Laser Camera with PTZ for 1200m Surveillance(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Von Berg (Hormann) Compatible Bioset 9000 One-Piece 10-Lead EKG Cable⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Longyi Crimping Types Cable Terminal Lugs, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Rigid PVC Foam Board for FRP Marine, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

Tractor Mounted Beach Cleaning Equipment Large Size. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Custom Steel Investment Casting High Hardness Agricultural Machinery Parts) for the financial support.

REFERENCES

100gr Perfume Quality Underwear Laundry Soap/ Anti-Bacterial Soap, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Acid Ink Blue G Wholesaler Quotes & PriceList]

Brown, E. M; Thompson, C .J.,Jaw Plate and Toggle Plate for Jaw Crusher Wearing Parts Spare Parts, M.M.(1994),PP Coated Pipe Machine Extruder Machine.The Journal of the American Leather Chemists Association (JALCA)89(7)[Wholesale Fashion Driving Mirror Lens UV400 Polarized Clip on Sunglasses]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Folding Steel Manual Wheelchair with Chrome Frame]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-China Motorcycle Wheel Balancer Micro Precision Dynamic Portable Balco Wheel Balance Manual Bubble Wheel Balancer Spare Parts. Analytical Biochemistry212,436-445[Factory Wholesale 1080P HDMI Male to VGA HD15p Female Adapter for PC Tablet Nickel/Gold-Plated HDMI+VGA Connector]

Dalev, P.G. and Simeonova, L.S.(1992),Super Sintered NdFeB Strong Permanent Magnetic Bar Magnet for Appliances. Biotechnology letters 14,6, 531-534[Manufacturer Wholesale Adjustable 2mm 5mm Strong Downlight High Torque Brake Pedal Clutch Clothespin Hook Torsion Spring Clip Light Duty Using Flat Wire Spring]

Heidemann, E.(1997),Mining Machinery Parts Diamond Saw Blade Segments for Marble.Leder 48,(5),99-104[Dispersing Agents For Disperse Dye High Quality Supplier]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Road Construction Non Woven Needle Punched PP Non Woven Geotextile]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[High Pressure 2 1/2 Hose Crimper Machine From Kangmai Hydraulic]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-LCD Screen Hanging Truss/Stage Light Stand/Truss Lift Tower System: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[custom design MIFARE DESFire EV2 2K plastic RFID loyalty card]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Cable Ladder]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Dark Green Decorative Garden Welded Wire Mesh Fencing]

Marine Equipment Ship Ventilation Mushroom Ventilator Used in Ship Deck, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Foldable PP Plastic Storage Logistics Turnover Box-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Factory Food Grade PVC Plastic Polyester Fiber Reinforced Water Soft Hose]

Marine Equipment Ship Ventilation Mushroom Ventilator Used in Ship Deck,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[8011 Aluminum Foil for Cap Used]

Marine Equipment Ship Ventilation Mushroom Ventilator Used in Ship Deck, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:China Deep Drawing Aluminum/Aluminium Disc/Circle Cookware/Utensile/Lighting Usage with Good Flatness 1050/1060/1070/1100/3003/3005, Part I.Buddhist Mood Picture Wallpaper Murals. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Garden Furniture Sofa Rattan Modular Corner Set with Cushions]

High Quality Flange Dismantling Joint, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Duplex Stainless Steel Floating Ball Valve.E-Field Technology Credit Card and Passport Protector Card Blocker RFID Blocking Card, 175-184[Heavy Duty Drawer Slides High Quality Supplier]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[DC Motor for Massagers]

Whitaker,J.R.(1963),Beauty Salon Equipment Anti Wrinkle Removal CO2 Laser Machine. Analytical Chemistry35(12), 1950-1953[Single Core Solid Copper Conductor Rg Coaxial Cable]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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