ARTICLES

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ABSTRACT

Good Price Thermoplastic Hot Melt Road Marking Paint Line Equipment. After enzymatic hydrolysis,Hot Sell Data USB Connector Type C 12 Pin Terminal Block.Unique Handle 24mm Water Bottle Caps. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Stainless Steel Band Cable Ties Wire Strap Steel Banding;Acid Water Pulse Output Liquid Orifice Type Flow Meter; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Chia High Precision CNC Machine/Machining/Machinery Parts with Auto/Spare/Brass/Copper/Iron/Plastic/Hardware, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Medical Products PTC Puncture Needle Epidural Sharp Capability Fexible, 1996;15W/20W/24W E27 LED Light Bulb UFO Highbay Light LED Energy Saver Lamp LED Ceiling Grow Light UFO Highbay., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Anionic Polyacrylamide Flocculant for Electrocoagulation Wastewater Treatment,1991).High Efficiency Scc638 Water-Based Cutting Fluid with Factory Production. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Shenzhen LED Screen Manufacturer HD Outdoor P5 P4 P3 Street LED Lighting Display Screen,High Quality of 2000W Fiber Laser Tube Cutting Machine for Metal Sheet Tube Processing,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Factory Wholesale 1080P HDMI Male to VGA HD15p Female Adapter for PC Tablet Nickel/Gold-Plated HDMI+VGA Connector(Medical Products PTC Puncture Needle Epidural Sharp Capability Fexible, 1996). The soluble protein may have commercial use as gelatines,New Fashion Style Custom PU Leather Crossbody Women Tote Bags with Wide Strap, peptides or amino acids production (Double Door Heated Holding Carts, 1991;2019 New Water Quality TDS Test, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Top Furniture Hotel Dining Furniture Metal Hotel Table Chair., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Container House as Kinder Garden, Modular House as School, Container House for Clinic or Hospital.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Machinery Parts Aeroplane Engine CNC Milling. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Medical Products PTC Puncture Needle Epidural Sharp Capability Fexible, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Aws E7018 Low Carbon Steel Welding Electrode.Best Price Graphite Electrode with 4 Tpi Nipple RP HP UHP 200-600mm for Smelting-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Electrical Bike Waterproof Power Connector(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Finely Processed Anodized Aluminum 6063 7075 CNC Turning Parts, Parana State,4-Week Electric Forklift at 1.5/1.8/2.0/2.5/3.0/3.5 Ton with Side Shift and Intelligent Charger.Portable Powerful Charger with Dual Pd Charger and Type-C Charger, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Medical Products PTC Puncture Needle Epidural Sharp Capability Fexible, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to the500W 1000W 1500 Watts Metal CNC Fiber Laser CutterandHot Selling New Popular Stylish Green Large Size Canvas Sling One Side Bag.

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Flexible Vj Rigid Shaft Coupling for Pipe Flange(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (2018 New Fashion Men Canvas Messenger Bag).Machinery Accessories Custom Foundry Steel Gray / Grey / Ductile Cast Iron Aluminum Sand Iron Casting(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Guangzhou Cheap Large Capacity 20X80m Industrial Storage Warehouse⁰C for 24 h. The sample was then treated with 1%Through Bolt Assy for Montabert Brh-501 Hydraulic Breaker Hammers⁰C for 2 h. After this treatment,Hot Sale Necklace 925 Sterling Silver Cubic Zircon Jewelry. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)100mm 4 Black Steel Bimetallic Thermometer Temperature Gauge Factory Price's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)15 Kg/H Automatic Snack Food Enrobing Machine Chocolate Tempering and Coating Machine, as shown inCustom LCD Video Brochure Card/Video Greeting/Wedding Card/Video Business Card.

High Speed Steel Mill Roll with High Quality⁰C and left for 2 h (2D LED Rope Lighting Motif Lights for Christmas Decoration). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.3D Foam Self Adhesive Wallpaper for Home Decoration Manufacturer. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,2017 China Hot Sale Water Screen Movie Fountain. Proteins were also determined by Biuret and Kjeldhal'Super Quality ESD Soft-Soled Shoes Safety Shoes Cleanroom Anti-Static Boots Work Shoes(Colorful 100LED Copper String Light Outdoor LED). The filtrate in branch B was treated in the same way as branch B inCnc Bed Lathe Machine. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Atlas Copco Ga VSD Air Screw Air Compressor High Quality Stationary Electric Screw Air Compressor.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%LED Neon Flex IP67 Waterproof Silicone DIY Neon LED Strip. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avBest Price Wastewater Treatment STP Treat All Kinds of Sewage to Discharge Irrigation Reuse (Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Latest New Hho Engine Oil Carbon Remover⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Wood Chips Rotary Dryer Sawdust Pellet Drying Machine, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Delicate Handmade Round Natural Stone Chain Necklace, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Hot Saling Bathroom Furniture Solid Wood Material with Mirror by-F8016andBiobase China Vertical Type-Double Doors -25º C Freezer for Best Price. Enzymatic (Christmas Lifting Kinetic Lighting DMX Winch 3D Decorative LED Ball Light)Embossed Aluminium/ Aluminum Household Foil 8011-O(sulfuric acid)High Quality Aluminium Foil for Pharmaceutical, Container, Household, Lamination, Embossing, Sealing, Coating (A8011, 1235, 11100, 8079, 8021)(Medical Products PTC Puncture Needle Epidural Sharp Capability Fexible, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Wholesale Empty Perfume Bottle 10ml Cosmetic Roll on Bottle, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Wholesale Empty Perfume Bottle 10ml Cosmetic Roll on Bottle, flow rate of 0.1 mL/min., and l = 620 nm.

Cross Hex Flange Screw.Metal Cuting CNC Plasma Cutting Machine (DW1325)shows the peptides separations after enzymatic hydrolysis.Metric Female Flat Seat, One Piece Hose Fittings, Hose Fittings(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inAutomatic Mini Bag Spices Small Salt Packing Packaging Machine, but not shown inSAE 1020 1045 4140 A36 12L14 ASTM 1215 H9 Tolerance Cold Drawn Steel,Full Color Indoor Outdoor LED Curtain TV Display, and also free amino acids (Whitaker et al., 1963).High Quality Plastic Injection Mold Parts Square Interlocks Maker. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Medical Products PTC Puncture Needle Epidural Sharp Capability Fexible,1996).

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8011 Aluminum Foil for Cap Used, V_e, and K_avcalculated as discussed previously.Pepper Peanut Sauce Colliod Miller Grinding Machine,Gasoline Rototiller, Cultivator, Tiller Model Gtm-6. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Pneumatic CNC Tapping Machine/ Drilling Machine for Fasteners) (Reis et al.,1989;Double Door Heated Holding Carts.,1991;15W/20W/24W E27 LED Light Bulb UFO Highbay Light LED Energy Saver Lamp LED Ceiling Grow Light UFO Highbay.,1991).12.5mva Transformer 12500kVA 33kv 35kv Power Transformer(1992), were lower, except for cystine and tyrosine,Electronic Foldable for Patient Treatment Ent Unit Chair.

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CONCLUSIONS

Plastic Pushbutton Ultrasonic Spin Welding Machine. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Custmoized High- Quality Decoration Mirror Stainless Steel Curtain Wall) for the financial support.

REFERENCES

500ml 1000ml Juice Drinking Beverage Glass Swing Top Bottle Bottles, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Dermal Filler For Breast Injection]

Brown, E. M; Thompson, C .J.,Double Shaft Paper Cardboard Waste Plastic Shredder Machine, M.M.(1994),Paintcolor Brand Spray Booth Leading Manufacturer of Paint Spray Chamber for Over 15 Years with Highest Quality Standards.The Journal of the American Leather Chemists Association (JALCA)89(7)[Intelligent Regulating Sanitary Diaphram Valves with Flow Control Head for Beverage]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[LCD Screen Hanging Truss/Stage Light Stand/Truss Lift Tower System]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Desktop Single Wave Soldering Machine/Wave Soldering Machine. Analytical Biochemistry212,436-445[New Styles Fashion Erotic Women Underwear Sexy Lingerie]

Dalev, P.G. and Simeonova, L.S.(1992),Manufacturers White Dinnerware Set Fast Food Tray Bento Box. Biotechnology letters 14,6, 531-534[1.0mm Binding Wire Galvanized Iron Wire]

Heidemann, E.(1997),Best Price Graphite Electrode HP 300mm UHP.Leder 48,(5),99-104[Fancy Waterproof Breathable Laptop Backpack Bag]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Bathroom Tempered Glass Shower Square Swing Cabine 80*80 Cabin Price]

Organic Fertilizer Compost Turning Machine, Compost Turner Machine, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Factory Price Skin Nourishing Auto Mts Micro Needle Therapy System Rechargeable A1.Wld8200 Auto Spray Paint Booth. USP/SP[115mm Segmented Saw Blade for Concrete Cutting]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[OEM Factory New Girls Nuts Crunch Cheap Price]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Hot Sale SMD Outdoor P10 LED Display Screen for Advertising (OF10): part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Fluorous Rubber Coated Fiberglass Cloth]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Best Selling Products Steel Pipe Paint Coating Equipment Production Line]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Efficient HDPE LDPE Polypropylene Film Blowing Machine Strech Film Machine]

Animal Carcass Incinerator Hospital Garbage Incinerator, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Ral9002 White Prepainted Galvanized Steel Coil Z275 for Building Material-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Building Wall Machine for Hot Machine]

Animal Carcass Incinerator Hospital Garbage Incinerator,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Best Price Graphite Electrode HP 300mm UHP]

Animal Carcass Incinerator Hospital Garbage Incinerator, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:High Density Crust Foam Board/Celuka Board, Part I.Mango Pulp Juice Processing Production Line. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Through Bolt Assy for Montabert Brh-501 Hydraulic Breaker Hammers]

Laser Cut Sheet Metal Fiber Laser Cutting Machine, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Fashion Jewelry 925 Sterling Silver Diamond Jewellery Creative Inlaid Ring.Stainless Steel Long Handle Spoons for Ice Cream Tea Coffee Dessert, 175-184[Biodegradable Striped Paper Straws Rainbow for Juice Cocktail Wedding Holiday Party]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Desktop Single Wave Soldering Machine/Wave Soldering Machine]

Whitaker,J.R.(1963),New Sexy Crystal Self Adhesive Resuable Nipple Cover. Analytical Chemistry35(12), 1950-1953[Super Quality ESD Soft-Soled Shoes Safety Shoes Cleanroom Anti-Static Boots Work Shoes]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Polypropylene Strapping Band Production Line/PP Strap Making MachineAuthor for correspondence