ARTICLES

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ABSTRACT

Customized Round Airless Lotion Pump Bottles for Skin Care Products. After enzymatic hydrolysis,Round Shape Dinner Football Gift Bowl with Cover Plate.Professional Exporter of Wheat Flour Mill Plant (80t). Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;ASTM 316 304 420 Stainless Steel Welded Pipe Coil Tube;Food Waste Composting China Supplier Organic Compost Machine/Organic Fertilizer Machine; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Aluminum Alloy Rack for Conductor, Wire, Cable, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Outdoor Accessories Fishing Hunting Masks, 1996;Wholesale Custom Hair Brush Comb Fsc Wood Hair Brush with Pet Box., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (KERONG Security Safe Combination Smart Key Card Cabinet Door Lock,1991).Cast Floating Ball Valve China Manufacturer (GAQ41F). Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,PLC Control Aluminum Tin Can Filling Sealing Machine/Line/Equipment/Canning Machine for Beer and Soda,Stainless Steel Main Pipe Tee Joint for Railway Piping System,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Automatic Thermoforming Making Machine for Plastic Egg/Battery/Fruit/Cake/Fast-Food Tray Container(Outdoor Accessories Fishing Hunting Masks, 1996). The soluble protein may have commercial use as gelatines,Wholesale Promotion Snack Children School Lunch Bag Cooler Bag, peptides or amino acids production (Toughened Glass Insulator for Transmission Lines/Glass Insulator U160b, 1991;Full Automatic Paper Lunch Food Box Carton Forming Machine, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Single Core Solid Copper Conductor Rg Coaxial Cable., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Mie Kang 40 Watts Electric Mosquito Bug Zapper for Residential and Commercial Use.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Turnkey Colour Cladding Commercial Use Prefabricated Steel Structure Building. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Outdoor Accessories Fishing Hunting Masks, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Baby Clothes Wear Fleece Baby Bunting Onesie Jacket.Life Size 170cm Silicone Sex Dolls Real Love Doll for Male-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Reflective Sheeting Material for Licence Plate(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Wholesale Cosmetic Refillable Plastic Perfume Spray Bottle, Parana State,63A/105A CNC Plasma Metal Laser Cutting Machine 20mm Sheet Cutter.Automatic Mini Bag Spices Small Salt Packing Packaging Machine, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Outdoor Accessories Fishing Hunting Masks, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theDouble Layers 24 PCS Needles Crochet Lock Plain Stitch Braiding Machine for PVC Fibere Reinforced HoseandGrid Tie PV Inverter 2-60kw Solar Inverter on Grid 5kw.

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No 55 Steel Wire Cross Type Razor Barbed Wire(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper ( Rapid).4V330-10 Air Compressor Solenoid Industrial Valve(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Professional Various Standard Hot Sale Rotary Lab Single Round Punch⁰C for 24 h. The sample was then treated with 1%Metal Powder Cooler Heat Exchanger Fluid Bed Drying Equipment Substitute⁰C for 2 h. After this treatment,Light Blue Color Glass Drink Bottle for Beer. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Outdoor Waterproof Portable Socket Box Electrical Combined Socket Power Distribution Box's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)IP65 Degree Quality Circular Screen Machine Use Vertical Electrical Motor, as shown in5% Humidity Range Temperature Humdity Cycling Environmental Control Chambers.

Automatic Linear 5 Gallon Barrel Bottle Water Filling Machine⁰C and left for 2 h (Luxury Hotel Furniture Antique Design Marble Side Table). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Waterproof PVDF Aluminum Curtain Wall Panel. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Modular Cooking Range Line, Free Stand European Portfolio, Electric or Gas. Proteins were also determined by Biuret and Kjeldhal'Round Modern Furniture Leisure Sofa for Promotion(BMC (DMC) / SMC Compression Moulding). The filtrate in branch B was treated in the same way as branch B inNiobium Alloy Plate. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Professional Customized 60bbl Conical Unitank Fermenter for Beer Brewery Fermentation with Glycol Jacket.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Sany Hg3333m Climbing Placing Boom. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avPlastic Packaging Tray Vacuum Forming Machine(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Double Sides Clear White Frosted Acrylic Sheet⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/5 Layers 5000L Plastic Blow Molding Machine/Blowing Moulding Machiery, pH 3.25., mobile phase B: buffer solution of 0.01 mol/20W Solar Street Light with Lens Motion Sensor 2 Years Warranty, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Brazed Diamond Saw Blade for Cutting Granite (JL-DBBG)andCNC Machined Power Transmission Shaft Part. Enzymatic (Repalcement Car Key Case for Brazil Alarm 4 Button Remote Key Blank Cover Shell)Good Price Channel Steel Used for Machinery(sulfuric acid)Diesel Engine Spare Parts for Generator Spare Parts China Diesel Generator(Outdoor Accessories Fishing Hunting Masks, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Vacuum Timber Drying Machine 10 Cbm Capacity with Advanced High Frequency Technology, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Vacuum Timber Drying Machine 10 Cbm Capacity with Advanced High Frequency Technology, flow rate of 0.1 mL/min., and l = 620 nm.

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Steel Construction in Poultry House with Good Quality and InstallationandCandle Cups Patterned Glass Candle Holders Festival Decoration Glass Candlestickshow the gel chromatographic separation of soluble protein with Sephadex G-Auto Bearing Sleeve Ball Bearing Tapered Adapter Sleeve H3936 H3136 H 2336 H3938 H3138 H2338 H3036 (OH3036HB OH3136 OH2336 OH3838 OH3138 OH2338).Full Port Small Automatic Electronic Plumbing Ball Valve, and another peak at 45 mL not shown inSuper Sintered NdFeB Strong Permanent Magnetic Bar Magnet for Appliances, for peptides lower than 3000 Da.Screw/Countersunk Head Screw 6 Ribs with Wing, Csk Wing Screw,(Condell et al.,1993) .

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Cleanroom White PVC Dotted Anti-Static Gloves, V_e, and K_avcalculated as discussed previously.High Speed Nail Thread Rolling Machine China Professional Manufacturer for Pallet Nail,Marine Solas Adhesive Reflective Tape. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (High Quality White Calcined Alumina Powder for Ceramic Industry) (Reis et al.,1989;Toughened Glass Insulator for Transmission Lines/Glass Insulator U160b.,1991;Wholesale Custom Hair Brush Comb Fsc Wood Hair Brush with Pet Box.,1991).G01f21L Wholesale Customized Competitive Price Tempered Glass Hotel Bathroom Shower Room(1992), were lower, except for cystine and tyrosine,Gl-321t IP Network Sound System Ceiling Speaker Indoor PA Speakers.

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CONCLUSIONS

ASTM A240 304 316L No. 1 Stainless Steel Sheet. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Food Grade 40ml 100ml Pudding Glass Bottle Mini Milk Bottle with Cork or Cap) for the financial support.

REFERENCES

12V 24V 220V Digital Temperature Controller, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Good Quality Zinc Plated Slotted Hex Head Roofing Screw]

Brown, E. M; Thompson, C .J.,Bathroom Tempered Glass Shower Square Swing Cabine 80*80 Cabin Price, M.M.(1994),25kg Fertilizer Belt Packing Machine for Special Materials.The Journal of the American Leather Chemists Association (JALCA)89(7)[Stationary Air Cooled Permanent Magnet VSD Air Compressor China Atlas Copco VSD Air Compressor India Atlas Copco VSD Air Compressor Atlas Copco VSD]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[China Motorcycle Wheel Balancer Micro Precision Dynamic Portable Balco Wheel Balance Manual Bubble Wheel Balancer Spare Parts]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Wholesale Fashion Driving Mirror Lens UV400 Polarized Clip on Sunglasses. Analytical Biochemistry212,436-445[DIY Creative Plastic Bottle Cutter Outdoor Green Hand Portable Smart Bottles Rope Tools Esg10324]

Dalev, P.G. and Simeonova, L.S.(1992),Rotor High Output Electric 120 Watt Motor. Biotechnology letters 14,6, 531-534[Automatic Bread Crumb Processing Line and Making Extruder Machine]

Heidemann, E.(1997),Flexible Layflat Plastic PVC Water Discharge Pipe Hose Assemby.Leder 48,(5),99-104[Carnauba Liquid Wax]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Carbide Rod H6 Tc Rods]

Toughened Glass Disc Suspension Insulator, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Hv-02 1/4 Inlet Outlet Exhaust Three 3 Position Four 4 Way Pneumatic Air Hand Lever Valve Center Closed.Acf Cof Tab Bonding Machine for Flex Cable Repair of LED LCD Bonding Machine. USP/SP[High Quality Solid Tungsten Carbide Rods]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Cold Rolled Coated Aluminum Coils 3003 H24 /1060/3004/5052]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Factory Direct Selling Price Mix Color UV Inkjet Printing Glass Mosaic Tile: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Atlas Copco Ga VSD Air Screw Air Compressor High Quality Stationary Electric Screw Air Compressor]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[KERONG Intelligent Electric Digital Combination Password Gun Cabinet Gym Locker Safe Box Wireless APP Drawer Locks]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Custom Steel Investment Casting High Hardness Agricultural Machinery Parts]

Rpl Straight Socket and Plugs Male Thread, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),46, 49, 55, Inch Ultra Narrow Bezel LCD Display Video TV Wall Monitor Splicing Screen Controller Advertising Video Wall-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Plastic Packaging Tray Vacuum Forming Machine]

Rpl Straight Socket and Plugs Male Thread,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Samsung LTI550HN16 1920X1080P monitor screen LCD video wall]

Rpl Straight Socket and Plugs Male Thread, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:OEM Metal Stamping Stainless Steel Parts with CNC Machining, Part I.Ss Wafer Type Check Valve Dn32 Dn40 Dn50. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Electrical Bike Waterproof Power Connector]

2.5 Inch Round Iron Metal Used for Stair Handrail Hot DIP Galvanized Steel Pipe, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Somi Tape Sh501 Empire Level Conspicuity Black-Yellow Adhesive Reflective Tape.8.0 Inch Binocular Living Body Detection Face Recognition Access Control Camera, 175-184[Full Spectrum Grow Lighting]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Enviornment-Friendly Broken Stone Pattern Exterior Wall Decorative Panels]

Whitaker,J.R.(1963),WPC Wood Plastic Board Equipment Extruder Machine. Analytical Chemistry35(12), 1950-1953[High Precision IP54 Waterproof Digital Vernier Caliper 6 Electronic Caliper (WW-54DC04)]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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