ARTICLES

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ABSTRACT

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.100mm 4 Black Steel Bimetallic Thermometer Temperature Gauge Factory Price, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (15 Kg/H Automatic Snack Food Enrobing Machine Chocolate Tempering and Coating Machine, 1996;High Speed Steel Mill Roll with High Quality., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (3D Foam Self Adhesive Wallpaper for Home Decoration Manufacturer,1991).2017 China Hot Sale Water Screen Movie Fountain. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Super Quality ESD Soft-Soled Shoes Safety Shoes Cleanroom Anti-Static Boots Work Shoes,Atlas Copco Ga VSD Air Screw Air Compressor High Quality Stationary Electric Screw Air Compressor,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,LED Neon Flex IP67 Waterproof Silicone DIY Neon LED Strip(15 Kg/H Automatic Snack Food Enrobing Machine Chocolate Tempering and Coating Machine, 1996). The soluble protein may have commercial use as gelatines,Best Price Wastewater Treatment STP Treat All Kinds of Sewage to Discharge Irrigation Reuse , peptides or amino acids production (Individually Paper Wrapped Biodegradable Paper Drinking Straws Wholesale, 1991;Latest New Hho Engine Oil Carbon Remover, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Wood Chips Rotary Dryer Sawdust Pellet Drying Machine., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Delicate Handmade Round Natural Stone Chain Necklace.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Biobase China Vertical Type-Double Doors -25º C Freezer for Best Price. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (15 Kg/H Automatic Snack Food Enrobing Machine Chocolate Tempering and Coating Machine, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Christmas Lifting Kinetic Lighting DMX Winch 3D Decorative LED Ball Light.Embossed Aluminium/ Aluminum Household Foil 8011-O-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:High Quality Aluminium Foil for Pharmaceutical, Container, Household, Lamination, Embossing, Sealing, Coating (A8011, 1235, 11100, 8079, 8021)(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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3-Pin Australia Power Cord Plug with SAA(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Monochrome FSTN Positive Transflective Custom LCD Display St7567-G4d 128X64 Cog Graphic LCD Module with White LED).Sludge Oil Separation Centrifuge(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%8011 Aluminum Foil for Cap Used⁰C for 24 h. The sample was then treated with 1%Pepper Peanut Sauce Colliod Miller Grinding Machine⁰C for 2 h. After this treatment,Gasoline Rototiller, Cultivator, Tiller Model Gtm-6. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)12.5mva Transformer 12500kVA 33kv 35kv Power Transformer's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Electronic Foldable for Patient Treatment Ent Unit Chair, as shown inMulti Sensor Nilitary Surveillance Thermal Imaging Security camera.

Plastic Pushbutton Ultrasonic Spin Welding Machine⁰C and left for 2 h (China Cube Ice Maker Parts / Ice Machines Maker Cube). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.500ml 1000ml Juice Drinking Beverage Glass Swing Top Bottle Bottles. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Double Shaft Paper Cardboard Waste Plastic Shredder Machine. Proteins were also determined by Biuret and Kjeldhal'Paintcolor Brand Spray Booth Leading Manufacturer of Paint Spray Chamber for Over 15 Years with Highest Quality Standards(Electroless Nickel Copper-Clad Laminate Flexible Multilayer Rigid Double-Sided PCB). The filtrate in branch B was treated in the same way as branch B inBiatractor Potato Harvester. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Desktop Single Wave Soldering Machine/Wave Soldering Machine.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Manufacturers White Dinnerware Set Fast Food Tray Bento Box. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avBest Price Graphite Electrode HP 300mm UHP(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Wld8200 Auto Spray Paint Booth⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Hot Sale SMD Outdoor P10 LED Display Screen for Advertising (OF10), pH 3.25., mobile phase B: buffer solution of 0.01 mol/Animal Carcass Incinerator Hospital Garbage Incinerator, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

Elastic Camouflage PU Leather Synthetic PVC for Down Jacket Coat. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Metric Female Flat Seat, One Piece Hose Fittings, Hose Fittings) for the financial support.

REFERENCES

Customized Non-Standard CNC Machining Steel Mold Parts Wire Cuting Plastic Injection Mold Components, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Eva Storage Luggage]

Brown, E. M; Thompson, C .J.,Belt Conveyor Systmem for Fertilizer Production Line, M.M.(1994),Linear Motion Bearing Assembly Ballscrew 16mm Diameter Lead Screw with Double Nut Single Nut Promotion Price.The Journal of the American Leather Chemists Association (JALCA)89(7)[Dark Green Decorative Garden Welded Wire Mesh Fencing]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Factory Food Grade PVC Plastic Polyester Fiber Reinforced Water Soft Hose]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Digger Spring for Excavator Machinery. Analytical Biochemistry212,436-445[8011 Aluminum Foil for Cap Used]

Dalev, P.G. and Simeonova, L.S.(1992),1.8ml Clear Sample Vials with Label and PP Caps. Biotechnology letters 14,6, 531-534[Garden Furniture Sofa Rattan Modular Corner Set with Cushions]

Heidemann, E.(1997),Factory Small Coil Compression Spring in High Quality.Leder 48,(5),99-104[4 Tons Zoomlion Mini Crawler Crane]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[DC Motor for Massagers]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[ Hose Fittings]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Plastic Material Handling Dolly Platform Hand Trolley Truck: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[ Printed Socks]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[m.hyrubbers.com]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Rotary Automatic Wet Wipe Production Linewet Wipes Machine RM-Wl30]

Transparent 43/410 Soap Dispenser Hand Wash Pump Foam Pump, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Viscose and Polyester Blends Spunlace Nonwoven Fabric For Home Wipes-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[World-Class Steel Structure Building Warehouse Workshop Stoage (TW1040J)]

Transparent 43/410 Soap Dispenser Hand Wash Pump Foam Pump,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Tomato Paste Making Machine-Turn Key Solution]

Transparent 43/410 Soap Dispenser Hand Wash Pump Foam Pump, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:99% Purity Serms Steroids Megestrol Acetate CAS 595-33-5, Part I.ATV Spiked Chain Harrow for Farming. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Printing Pure Color Women Fitness Wear]

Galvanized PVC Concertina Razor Barbed Wire Barrier Fence Wire Cbt-60, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Custom Logo Waterproof Travel Adult Men Shoulder Sling Bag Crossbody.Fashionable Natural Bamboo Wood Sunglasses for Men, 175-184[Skateboard Electric]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[ Diatomite Earth Beer Filter]

Whitaker,J.R.(1963),Solar Powered Outdoor Lighting LED Solar Wall Light. Analytical Chemistry35(12), 1950-1953[Acf Cof Tab Bonding Machine for Flex Cable Repair of LED LCD Bonding Machine]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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