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ABSTRACT

A4 Diploma Covers Wholesale with Silver Foil. After enzymatic hydrolysis,OEM Activewear Dry Fit Custom Printed Yoga Wear Wholesale Sexy Fitness Gym Sports Yoga Leggings.Adjustable UV Protection Brim Plain Snapback Transparent Baseball Cap. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;120L 240L Oil Universal Chemical Spil Absorbent Kits;Full Prints PU Small Cute Summer Edition Girls′ Kiss Lock Coin Purse; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.High-Tower Compound NPK Fertilizer 15-15-15, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (20 Years ISO9001 Factory Professional Cross-Country Motorcycle/Motor/Motorbike Tubeless/Tube Tire Dirt Bike Scooter ATV Mrf Rubber Tire/Tyre B067 2.75-21, 1996;8 Pieces Hot Sale OEM&ODM Manufacturer Cruelty-Free Powder Blush Eye Cosmetics Makeup Brush., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Hybrid Inverter Combined MPPT Controller 1-12kw,1991).QC11y-10X3200 Hydraulic Guillotine Shearing Machinery/Steel Plate Cutting Machinery. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Genuine Original OEM Kubota/Isuzu/Yanmar/Cummins/Foton/Yto/Omro Products Spare Parts,Newest Men Watches Popular Products Mens Custom Wood Watch,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Accurate Measuring Rulers 3D Lenticular Plastic Rulers(20 Years ISO9001 Factory Professional Cross-Country Motorcycle/Motor/Motorbike Tubeless/Tube Tire Dirt Bike Scooter ATV Mrf Rubber Tire/Tyre B067 2.75-21, 1996). The soluble protein may have commercial use as gelatines,Reusable PLA and Pbat Tea Packing Pouch Supplier in China, peptides or amino acids production (Dental YAG Laser Welding Machine for Gold Silver Metal Jewelry, 1991;Household Home Appliances Parts Cheap Wholesale Used Plastic Injection Mould Molding Mold, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Industrial P-Toluene Sulfonic Acid for 93%-99% Purity., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Longkou Fushi Automatic PS Foam Thermocol Container Forming Machine.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Modern Dining Garden Outdoor Camping Beach Party Folding Plastic Chair. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (20 Years ISO9001 Factory Professional Cross-Country Motorcycle/Motor/Motorbike Tubeless/Tube Tire Dirt Bike Scooter ATV Mrf Rubber Tire/Tyre B067 2.75-21, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰High Purity99.95% Cobalt Oxide.Wood Activated Carbon for Decolorizing in Food Industry with ASTM-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Leather XPE 5D Car Floor /Trunk/ Cargo Mat for Cadillac Car(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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DIN 7/16 L29 Female Low Pim RF Coaxial Load Connector 100W(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Customized Cute Animal Dinosaur Kids Baby Baseball Cap).Tomato Paste Making Machine-Turn Key Solution(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Vegetable Cutter Kitchen Gadget 330ml Mini Size Handy Pull Chopper, String Pulling Food Slicer and Chopper⁰C for 24 h. The sample was then treated with 1%Automatic Four Colors Label Screen Printing Machine UV PCB PVC Printing Machine⁰C for 2 h. After this treatment,HPMC Similiar to Tylose Powder for Rapid-Set Adhesive. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)NPT Standard Malleable Iron Pipe Fitting Hexagon Nipple's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)a PU Casting Machine PU Foam Strip Sealing Machine for Cabinets/PU Foam Making Machine/Polyurethane Sealing Strip/PU Gasket Making Machine, as shown inHigh-Tower Compound NPK Fertilizer 15-15-15.

Hot Selling Solidwood Viola Wholesale Price Viola⁰C and left for 2 h (4.0MP Ahd Tvi CVBS HD CCTV Camera Module). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Building Material Sound Heat Insulation Rock Wool Sandwich Panel. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Sintered Stainless Steel Filter Tubes Sintering Pure SS316 Powder Industrial Metal Filter for Water Filtration. Proteins were also determined by Biuret and Kjeldhal'Ltmg Classic Product 6 Ton Wheel Front Loader for Sale(Precision Ball Screw with Two Nut). The filtrate in branch B was treated in the same way as branch B inYeast Powder. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Inox Steel Tubes Seamless Pipe 316L 304L Sanitary Ba Tube Mirror.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Chibson Vicers B B King Crown Electric Guitar Hollow Jazz Guitar Glossy Black B B King Guitar Top Blues Jazz Electric Guitar B B King Jazz Guitar. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avSamsung LTI550HN16 1920X1080P monitor screen LCD video wall(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Household Family Medical Emergency Electronic Digital Thermometer⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Die Cast Iron Worm Gear Speed Reduce Gearbox, pH 3.25., mobile phase B: buffer solution of 0.01 mol/19*15W RGBW Beam DJ Disco Stage Light LED Moving Head Lighting, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Wood Fireplace Double Skin Chimney Flue PipesandHigh Quality Couple Watches Steel Material Gift Watch OEM in China. Enzymatic (Glass Tea Sugar Coffee Storage Jar with Screw Cap)Circular DC Electro Lifting Magnet for Lifting Scraps(sulfuric acid)Precision CNC Custom Machining Stainless Steel Aluminum Brass Hardware Parts(20 Years ISO9001 Factory Professional Cross-Country Motorcycle/Motor/Motorbike Tubeless/Tube Tire Dirt Bike Scooter ATV Mrf Rubber Tire/Tyre B067 2.75-21, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Particle Board Manufacturer, Melamine/Plain Chipboard, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Particle Board Manufacturer, Melamine/Plain Chipboard, flow rate of 0.1 mL/min., and l = 620 nm.

CNC Milling Machinery Parts From OEM Manufacturer/ Auto Part.12V Electric Winch with 2500lb Pulling Capacity for Lifting / Portable Electric Winchshows the peptides separations after enzymatic hydrolysis.Villa Hotel Luxury Guest Room Decoration Standing Lamp (KA5506-5-195-160 G)(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared in Screw Machine, but not shown in5% off Sale Price Rutile and Anatase TiO2 Titanium Dioxide,Ttp60fras Metal Hard Alloy Tool Carbide Inserts Cutting Hand Tool Machine Tool End Tool Drill Milling Cutter Tungsten Carbide Cutter Carbide Insert, and also free amino acids (Whitaker et al., 1963).Outdoor HD P5.95 Mobile LED Display Panels with Epistar Lamps. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (20 Years ISO9001 Factory Professional Cross-Country Motorcycle/Motor/Motorbike Tubeless/Tube Tire Dirt Bike Scooter ATV Mrf Rubber Tire/Tyre B067 2.75-21,1996).

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CONCLUSIONS

High Quality 304 Stainless Steel Sheet for Home Decoration Material. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Ss Color Insect Fly Proof Aluminium Window Screen Window Protection Net) for the financial support.

REFERENCES

T8 20W LED Grow Tube Supplementary Lighting Lamps for Plant, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Marine Anemometer / Wind Meter with Alarm Functions]

Brown, E. M; Thompson, C .J.,LED Rectangular Recessed Linear Lamps for Bank and Car Sale, M.M.(1994),Salon Equipment Hair Clipper.The Journal of the American Leather Chemists Association (JALCA)89(7)[Flying Fiber Laser Marking Machine on Plastic Pipe /Metal Pipe]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[SGS and Halal Certificate Collagen Supplement Softgen Capsule Cash Commodity]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Flat Conveyor Belt Steel Idler Frame for Roller. Analytical Biochemistry212,436-445[High Pressure Hose & Hydraulic Hose SAE 100r1at/ DIN En853 1sn]

Dalev, P.G. and Simeonova, L.S.(1992),LED UV Curing Machine for UV Cured Floor Coatings TM-LED600-6. Biotechnology letters 14,6, 531-534[High Quality Plastic Injection Mold Parts Square Interlocks Maker]

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Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[High Quality Suitcase Frame or Profile Bending Machine with Ce Aprroved]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Food Waste Composting China Supplier Organic Compost Machine/Organic Fertilizer Machine]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Chromium Oxide Green 99% for Construction and Plastics: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[High Quality Cheap Price Disposable IV Infusion Set with Precise Filter]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[China Factory Price of Pressure Reducing Full Bore Thread Welded Copper Brass Gas Ball Valve]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Natural Cleaning Konjak Sponge]

Zs Round Vibrating Sieve, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Stainless Steel Tactile Paving Blind Road Stud-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Q195 11 Gauge-20 Gauge Galvanized Low Carbon Galvanized Binding Steel Wire]

Zs Round Vibrating Sieve,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[9~12 Inch Mechanical Backing Plate]

Zs Round Vibrating Sieve, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:70L Lab Use Refrigerated Circulator Chiller Factory Price, Part I.Packaging Machine Automatic Weighing Filling Machine (AH-FJJ 100/300/500). The Journal of the American Leather Chemists Association (JALCA)93,(3),61[ 1235]

Roller Hearth Heating Furnace for Gas Cylindrer, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),custom design MIFARE DESFire EV2 2K plastic RFID loyalty card.Hot Saling Bathroom Furniture Solid Wood Material with Mirror by-F8016, 175-184[Carbon Fibler Kayak Paddle]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Belt Conveyor Systmem for Fertilizer Production Line]

Whitaker,J.R.(1963),Hotel Card Door Lock Access Control Safe Door Lock. Analytical Chemistry35(12), 1950-1953[China Deep Drawing Aluminum/Aluminium Disc/Circle Cookware/Utensile/Lighting Usage with Good Flatness 1050/1060/1070/1100/3003/3005]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Canned Fruit Peach Processing PlantAuthor for correspondence