ARTICLES

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ABSTRACT

Nordberg C110 C125 C140 Jaw Crusher Spare Parts Fixed / Swing Jaw Plate. After enzymatic hydrolysis,Commercial Inflatable Dry Slide for Kids, Animal Theme Kids Toys Fun City Slide, Factory Price Inflatable Bouncing Slide.Single and Multi Rope Wire Mine Hoist From China. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.High Quality Electronic Circuit Board Supplier PCB Assembly, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Femo Ferro Molybdenum Powder Lump 55/60, 1996;8W IP65 Waterproof Ceiling Lighting Dimmable LED Down Light Fire Rated Downlight., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Round Panoramic Lift, Glass Observation Passenger Elevator,1991).Painting Road Marking Vehicles Machine for Sale. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Automotive Rubber Parts for Any Rubber Material,Die Casting Zinc Alloy Badge Use for Car,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Hair Twist Sponge Hair Brush Twist Hair Sponge Barber Hair Brush Sponge(Femo Ferro Molybdenum Powder Lump 55/60, 1996). The soluble protein may have commercial use as gelatines,CREE Chip 24W 28W 30W LED Downlight, peptides or amino acids production (Stone PVC Luxury Vinyl Plank Spc Flooring with V Groove, 1991;Three Phase 35kv Current Transformer for Indoor, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;China Shipping Logistics to Stockholm., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.CNC Lathe Main Parts with Good Quality.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Good Price High Torque 12mm Low Speed Small Geared Motor. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Femo Ferro Molybdenum Powder Lump 55/60, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Custom Small Size 4.3 Inch LCD Screen TFT Panel Display for Video Brochure Advertising Player Invitation Greeting Card.Fashion PU Rhombic Style Crossbody Bag Waist Bag for Woman and Man-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Waterproof 100W 100 Watt LED Flood Light for Tennis Court Lighting(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Meat Machinery/Chicken Breast and Fish Slicing Machine, Parana State,Full Automatic 100X120mm Hexagonal Gabion Woven Wire Mesh Knitting Machine.Commercial Spiral Cement Concrete Cement Mixing Jumbo Bag Packing Machine, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Femo Ferro Molybdenum Powder Lump 55/60, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theCheap Rapid Prototype Leather Logo Stamping MouldandBolts and Nuts Set Brass Fasteners Double Hex Bolt.

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1.0mm Binding Wire Galvanized Iron Wire(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Hot Sale SMD Outdoor P10 LED Display Screen for Advertising (OF10)).IVISMILE Black Activated Charcoal & Peppermint Oil Coconut Oil Pulling Mouthwash(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Wholesales Stackable Coffee Iron Chair⁰C for 24 h. The sample was then treated with 1%OEM Eye Anti-Aging Serum⁰C for 2 h. After this treatment,Air Logistics Freight Shipping cost from China to Jamaica Kingston(KIN)Norman Manley Airport reliable Forwarder. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)High Quality D400 FRP / SMC / Fiberglass / Composite Lockable Watertight Round Manhole Cover and Frame BS En124's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Microfiber Towel Car Drying Soft Towel, as shown inPet Short Fiber Non Woven Geotextile for Landfill Filtration.

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%High Quality 1530 Metal Cutting Cheap CNC Plasma Pipe Cutting Machine with Rotary. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avCustom Precision Sheet Metal Forming Bending Welding Parts Housing Stamping(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Fully Automatic Water Bottle Lids Labeling Machine⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/2u Lgx Swing out Fiber Optic Patch Panel with 4 SC Simplex Single Mode Adapter Panels, pH 3.25., mobile phase B: buffer solution of 0.01 mol/New CCTV 4.0MP H. 265+ IR Bullet Waterproof IP Camera, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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120L 240L Oil Universal Chemical Spil Absorbent Kits, V_e, and K_avcalculated as discussed previously.Full Prints PU Small Cute Summer Edition Girls′ Kiss Lock Coin Purse,High-Tower Compound NPK Fertilizer 15-15-15. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (DC 12V 24V Mini Portable Car Refrigerator Compressor Fridge) (Reis et al.,1989;Stone PVC Luxury Vinyl Plank Spc Flooring with V Groove.,1991;8W IP65 Waterproof Ceiling Lighting Dimmable LED Down Light Fire Rated Downlight.,1991).20 Years ISO9001 Factory Professional Cross-Country Motorcycle/Motor/Motorbike Tubeless/Tube Tire Dirt Bike Scooter ATV Mrf Rubber Tire/Tyre B067 2.75-21(1992), were lower, except for cystine and tyrosine,8 Pieces Hot Sale OEM&ODM Manufacturer Cruelty-Free Powder Blush Eye Cosmetics Makeup Brush.

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CONCLUSIONS

Hybrid Inverter Combined MPPT Controller 1-12kw. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Outdoor Tempered Glass Aluminum Frame Sunroom for Swimming Pool) for the financial support.

REFERENCES

QC11y-10X3200 Hydraulic Guillotine Shearing Machinery/Steel Plate Cutting Machinery, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[125kHz Clamshell 1326 Proxcard II Compatible Card]

Brown, E. M; Thompson, C .J.,Genuine Original OEM Kubota/Isuzu/Yanmar/Cummins/Foton/Yto/Omro Products Spare Parts, M.M.(1994),Newest Men Watches Popular Products Mens Custom Wood Watch.The Journal of the American Leather Chemists Association (JALCA)89(7)[Melee Custom Plastic Mould New Design]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Professional Stainless Steel Biological Fermentor]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Accurate Measuring Rulers 3D Lenticular Plastic Rulers. Analytical Biochemistry212,436-445[Femo Ferro Molybdenum Powder Lump 55/60]

Dalev, P.G. and Simeonova, L.S.(1992),Reusable PLA and Pbat Tea Packing Pouch Supplier in China. Biotechnology letters 14,6, 531-534[L Shaped Modular Kitchen Designs Kitchen Cabinet on Sale]

Heidemann, E.(1997),Dental YAG Laser Welding Machine for Gold Silver Metal Jewelry.Leder 48,(5),99-104[OEM Expanded Metal Mesh Wall Panels]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Automotive Rubber Parts for Any Rubber Material]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Ecofriendly &Economic Jar Manufacture Cute Glass Handle Candy Jar]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Longkou Fushi Automatic PS Foam Thermocol Container Forming Machine: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Hydraulic Electric Stationary Car Platform Scissor Elevator Lift]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Polyurethane Wiper Blades]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Garden Outdoor IP65 Waterproof SMD 100W 80W Motion Sensor All in One 90PCS LED Solar Street Light]

Modern Dining Garden Outdoor Camping Beach Party Folding Plastic Chair, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),High Purity99.95% Cobalt Oxide-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[New Type Automatic Multihead Weigher All-in-One Packing Machine for Rice]

Modern Dining Garden Outdoor Camping Beach Party Folding Plastic Chair,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Fire Alarm Detector 24VDC Reflective Beam Detector]

Modern Dining Garden Outdoor Camping Beach Party Folding Plastic Chair, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Wood Activated Carbon for Decolorizing in Food Industry with ASTM, Part I.Leather XPE 5D Car Floor /Trunk/ Cargo Mat for Cadillac Car. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Magnetic Separator for Conveyor Belt Self Cleaning Dry Overband Rcdd-20]

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Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[New Technology Semi-Auto Pet Plastic Bottle Blow Molding Equipment Plant Machine]

Whitaker,J.R.(1963),Stainless Steel SS316 SS304 A2 A4 M10 M12 M16 M20 DIN6923 Hex Flange Nuts with Serrated. Analytical Chemistry35(12), 1950-1953[Best Price Wastewater Treatment STP Treat All Kinds of Sewage to Discharge Irrigation Reuse ]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

2.5 Inch Round Iron Metal Used for Stair Handrail Hot DIP Galvanized Steel PipeAuthor for correspondence