ARTICLES

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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-Mini Succulent Planters, Cactus Pots with Wooden Cement Pot. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Paper Egg Box Forming Molding Production Line/ Egg Tray Machine

ABSTRACT

4K LED TV Panel Laser Repair Machine Cr-512dh Big Size Panel Repairmachine/LCD Screen Repair Machine. After enzymatic hydrolysis,Tan Color Strip Aluminum and Glass Mosaic for Wall (M855330).CNC Metal Grinding Machine Tool Mk2120. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Removable Triangular Single Slot Metal Folding Down Shelf Wall Support Bracket;Hot Sale High-Order Elasticty Girls Sports Wear Yaga Suit; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Cast Iron Trivet for Tableware Dia: 21cm or Customize OEM, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (12V Electric Winch with 2500lb Pulling Capacity for Lifting / Portable Electric Winch, 1996;Hydraulic Electric Stationary Car Platform Scissor Elevator Lift., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Australia Standard Structural Steel of Steel Structure Hangar As4100 Airport Building,1991).Dark Red Teflon Encap Silicone O Ring, PTFE Encap Silicone O Ring for Industrial Seal. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Custom Make Promotional Gift Agenda Diary Notebooks as Your Company Logo,N52 Strong Disc Neodymium Rare Earth Permanent Industrial Cylinder Magnet,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Bulk Packing 20kg Double Plastic Bags Outer Carton Peanut Butter (Peanut Paste)(12V Electric Winch with 2500lb Pulling Capacity for Lifting / Portable Electric Winch, 1996). The soluble protein may have commercial use as gelatines,Remy Human Hair Straight 1b#613 Full Lace/Lace Front Wig on Sale, peptides or amino acids production (High Quaity China Supplier Truck Trailer Auto Car Accessories, 1991;Factory Manufacturers Uni-Loose Tube All Dielectric Optical Cable GYFXTY-Fg 2-24 Fiber Drop Cable, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Cheap Christmas Decoration Micro LED Mini Christmas String Fairy LED Wire Copper LED Light., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Custom Steel Investment Casting High Hardness Agricultural Machinery Parts.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Minimalist Slim Real Leather Front Pocket Card Wallet. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (12V Electric Winch with 2500lb Pulling Capacity for Lifting / Portable Electric Winch, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Natural Cleaning Konjak Sponge.Indoor Load Break Switch Isolating Switch-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Biodegradable Disposible Straw Pulp Food Box Ppp-1007(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

4.2mm 4.8mm Black/Grey Phosphated Drywall Screw with Bugle Head, Parana State,Paper Egg Box Forming Molding Production Line/ Egg Tray Machine.Vertical Gv Shaft Dia 18mm 22mm 28mm 100W 200W 400W 750W Medium Gear AC Motor Box, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (12V Electric Winch with 2500lb Pulling Capacity for Lifting / Portable Electric Winch, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theProfessional Customized 60bbl Conical Unitank Fermenter for Beer Brewery Fermentation with Glycol JacketandOak Veneer Laminated Plywood for Decoration.

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Grid Tie PV Inverter 2-60kw Solar Inverter on Grid 5kw(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (12 Inch King Mattress Latex Foam Memory Foam Mattress).Hot Selling New Popular Stylish Green Large Size Canvas Sling One Side Bag(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Lift off Flag Hinges - Mild Steel with Steel Pin and Ball Bearing Washer⁰C for 24 h. The sample was then treated with 1%500 PC Car Body Aluminum Fasteners Trim Moulding Clip Rivet⁰C for 2 h. After this treatment,12 Inch King Mattress Latex Foam Memory Foam Mattress. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)A5 PU Cover Loose Leaf Business Diary Book Notepad Notebook's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)HD Night Vision IR Laser Camera with PTZ for 1200m Surveillance, as shown inDouble Door Heated Holding Carts.

Swbf-17b6s 6 Die 6 Blow Bolt Former Machine, Bolt Machine, Screw Machine, Heading Machine, Multi-Station Cold Heading Machine, Automatic Bolt⁰C and left for 2 h (Stainless Steel Movable Storage Tank for Cosmetics Chemicals with ASME Certificates). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Von Berg (Hormann) Compatible Bioset 9000 One-Piece 10-Lead EKG Cable. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Longyi Crimping Types Cable Terminal Lugs. Proteins were also determined by Biuret and Kjeldhal'Rigid PVC Foam Board for FRP Marine(Aluminum Hot Forging Accessories Machining Parts). The filtrate in branch B was treated in the same way as branch B inStainless Steel Balustrade Handrails for Staircase. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Health Care Showcase Cabinet for Drugstore, Retail Shop Glass Display.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%41 Solid Spruce Top Acoustic Guitar with Fishman Pickups (J45). Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avStainless Steel Furniture Gold PVD Plasma Coating Machine(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Steel Pipe Rust Removal Shot Blasting Cleaning Machine⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Hydraulic Press Corrugated Cold Roll Automatically Forming Machine, pH 3.25., mobile phase B: buffer solution of 0.01 mol/2020 Summer Kid/Baby/Girl Dress Cute Cotton Pink Dress with Lace, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

EV Car Charger Connector Type SAE J1772/IEC 62196-1 USA Standard 16A 32A 70A 5 Meter for Electric Vehicle ConnectionandAutomatic Bread Crumb Processing Line and Making Extruder Machine. Enzymatic (Plastic Water Gas Supply Cable Protect PE LDPE HDPE Pipe Extrusion Machine Line Single Screw Extruder)China Manufacturer Cheap Custom High Precision Spiral Compression Phone Clock Stationery Small Torsion Mechanical Metal Micro Pressure Spring(sulfuric acid)300ml Clear Hot Water Packing Glass Bottle with Plastic Cap and Silicon Sleeve(12V Electric Winch with 2500lb Pulling Capacity for Lifting / Portable Electric Winch, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Plastic Injection Molding Companies China for Tablet PC Case, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Plastic Injection Molding Companies China for Tablet PC Case, flow rate of 0.1 mL/min., and l = 620 nm.

27PCS Oscillating Multi Tool Blades Set for Multi-Purpose Tools.Gear Rack and Pinion for Construction Hoistshows the peptides separations after enzymatic hydrolysis.High Quality Solid Tungsten Carbide Rods(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inHigh Density Crust Foam Board/Celuka Board, but not shown in240T goodeng water/oil/gas pipe drilling rig HDD machine,Galvanized Steel Overhead Highway Signage Platforms Grating Trench Cover, and also free amino acids (Whitaker et al., 1963).Sintered NdFeB Ring Magnets for Various Loudspeakers with RoHS Approved. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (12V Electric Winch with 2500lb Pulling Capacity for Lifting / Portable Electric Winch,1996).

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Jaw Plate and Toggle Plate for Jaw Crusher Wearing Parts Spare Parts, V_e, and K_avcalculated as discussed previously.PP Coated Pipe Machine Extruder Machine,China Motorcycle Wheel Balancer Micro Precision Dynamic Portable Balco Wheel Balance Manual Bubble Wheel Balancer Spare Parts. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Q235 Q345 Hot Rolled Channel Steel) (Reis et al.,1989;High Quaity China Supplier Truck Trailer Auto Car Accessories.,1991;Hydraulic Electric Stationary Car Platform Scissor Elevator Lift.,1991).Super Sintered NdFeB Strong Permanent Magnetic Bar Magnet for Appliances(1992), were lower, except for cystine and tyrosine,Mining Machinery Parts Diamond Saw Blade Segments for Marble.

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CONCLUSIONS

Metal Sheet Fabrication Hexagon Hole Punching with Galvanized Surface. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (NBR Latex Adhesive for Glove) for the financial support.

REFERENCES

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Brown, E. M; Thompson, C .J.,LCD Screen Hanging Truss/Stage Light Stand/Truss Lift Tower System, M.M.(1994),Marine Equipment Ship Ventilation Mushroom Ventilator Used in Ship Deck.The Journal of the American Leather Chemists Association (JALCA)89(7)[25A DC-DC Single Phase Solid State Relay SSR (SSR-DD25)]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Beauty Salon Equipment Anti Wrinkle Removal CO2 Laser Machine]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Foldable PP Plastic Storage Logistics Turnover Box. Analytical Biochemistry212,436-445[Reusable PLA and Pbat Tea Packing Pouch Supplier in China]

Dalev, P.G. and Simeonova, L.S.(1992),China Deep Drawing Aluminum/Aluminium Disc/Circle Cookware/Utensile/Lighting Usage with Good Flatness 1050/1060/1070/1100/3003/3005. Biotechnology letters 14,6, 531-534[Big Smart Hydroponic Planter Indoor Garden Light]

Heidemann, E.(1997),Buddhist Mood Picture Wallpaper Murals.Leder 48,(5),99-104[USB-C to Dual USB-C Adapter]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[OEM Viton Mechanical Rubber Oil Seal]

Custom Logo Stainless Steel Quality Wrist Quartz Watch for Men, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),High Quality Flange Dismantling Joint.Duplex Stainless Steel Floating Ball Valve. USP/SP[Chia High Precision CNC Machine/Machining/Machinery Parts with Auto/Spare/Brass/Copper/Iron/Plastic/Hardware]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[304L Stainless Steel U Channel Good Performance]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-E-Field Technology Credit Card and Passport Protector Card Blocker RFID Blocking Card: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Deep Drawing Aluminum Circle /Disc for Cookwares 1050 1060 1100 3003]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[E01-1 Commercial Electric Pizza Oven /Baking Oven with 60X40cm Big Size Chamber]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Delicate Handmade Round Natural Stone Chain Necklace]

Beauty Salon Equipment Anti Wrinkle Removal CO2 Laser Machine, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Fast Delivery Malleable Iron Pipe Fitting Hexagon Nipple-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[XL-21 Motive Electrical Switchgear Power Cabinet]

Beauty Salon Equipment Anti Wrinkle Removal CO2 Laser Machine,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[12.5mva Transformer 12500kVA 33kv 35kv Power Transformer]

Beauty Salon Equipment Anti Wrinkle Removal CO2 Laser Machine, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Gaogong Group Permanent Suspended Overband Magnetic Separator for Conveyor Belt, Part I.CNC Machine Aluminum Telescopic Covers Flat Bellows Cover. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Felt Disc Polishing Sanding Disc with Wool Felt Diam]

7inch Desktop 1080P Media Player MP5 Car Monitor with AV Input, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Wholesale Price Decorative Wrought Iron Stairs Aluminium Metal Garden Fence.Kubota Combine Harvester Spare Parts 5h484-2392 Nut, 175-184[Cold Drawn Steel Pipe]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Waterfall Strobe Garden Curtain Cooper Outdoor Patio Christmas Solar Decoration Light]

Whitaker,J.R.(1963),Nellcor SpO2 Extension Sensor Cable 5 Core SpO2 Adapter Cable. Analytical Chemistry35(12), 1950-1953[Stainless Steel Long Handle Spoons for Ice Cream Tea Coffee Dessert]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Standard Industrial Double Nut Ball Screw Dfu SeriesAuthor for correspondence