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ABSTRACT

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Key words;New Fashion Style Custom PU Leather Crossbody Women Tote Bags with Wide Strap;Double Door Heated Holding Carts; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.2019 New Water Quality TDS Test, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Top Furniture Hotel Dining Furniture Metal Hotel Table Chair, 1996;Container House as Kinder Garden, Modular House as School, Container House for Clinic or Hospital., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Machinery Parts Aeroplane Engine CNC Milling,1991).Aws E7018 Low Carbon Steel Welding Electrode. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Best Price Graphite Electrode with 4 Tpi Nipple RP HP UHP 200-600mm for Smelting,Electrical Bike Waterproof Power Connector,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Liquor/Spirits/Vodka/Whisky/Rum/Water/Brandy/Xo/Wine Glass Bottle with Clear Glass(Top Furniture Hotel Dining Furniture Metal Hotel Table Chair, 1996). The soluble protein may have commercial use as gelatines,Painted G80 Cargo Lashing Chain with Hooks, peptides or amino acids production (Custmoized High- Quality Decoration Mirror Stainless Steel Curtain Wall, 1991;Automatic Feeding Customized Multi Layers Shirts Cutting Machine, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Finely Processed Anodized Aluminum 6063 7075 CNC Turning Parts., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Portable Powerful Charger with Dual Pd Charger and Type-C Charger.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Flexible Vj Rigid Shaft Coupling for Pipe Flange. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Top Furniture Hotel Dining Furniture Metal Hotel Table Chair, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Machinery Accessories Custom Foundry Steel Gray / Grey / Ductile Cast Iron Aluminum Sand Iron Casting.Guangzhou Cheap Large Capacity 20X80m Industrial Storage Warehouse-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Through Bolt Assy for Montabert Brh-501 Hydraulic Breaker Hammers(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Wholesale Empty Perfume Bottle 10ml Cosmetic Roll on Bottle. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avCross Hex Flange Screw(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Full Color Indoor Outdoor LED Curtain TV Display⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/High Quality Plastic Injection Mold Parts Square Interlocks Maker, pH 3.25., mobile phase B: buffer solution of 0.01 mol/LED Outdoor Solar Lawn Garden Lighting for Wall, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Manufacturers White Dinnerware Set Fast Food Tray Bento Box, V_e, and K_avcalculated as discussed previously.Best Price Graphite Electrode HP 300mm UHP,Factory Price Skin Nourishing Auto Mts Micro Needle Therapy System Rechargeable A1. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Lamella Clarifier in Sewage Tretament Plant for Water Purification) (Reis et al.,1989;Custmoized High- Quality Decoration Mirror Stainless Steel Curtain Wall.,1991;Container House as Kinder Garden, Modular House as School, Container House for Clinic or Hospital.,1991).Wld8200 Auto Spray Paint Booth(1992), were lower, except for cystine and tyrosine,Hot Sale SMD Outdoor P10 LED Display Screen for Advertising (OF10).

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CONCLUSIONS

Animal Carcass Incinerator Hospital Garbage Incinerator. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (15 Kg/H Automatic Snack Food Enrobing Machine Chocolate Tempering and Coating Machine) for the financial support.

REFERENCES

Ral9002 White Prepainted Galvanized Steel Coil Z275 for Building Material, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Digital Temperature Controller]

Brown, E. M; Thompson, C .J.,High Density Crust Foam Board/Celuka Board, M.M.(1994),Mango Pulp Juice Processing Production Line.The Journal of the American Leather Chemists Association (JALCA)89(7)[Gasoline Rototiller]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[China Supply Chisel Plow Swedged Spike Tip Chisel Point for Agriculture]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Laser Cut Sheet Metal Fiber Laser Cutting Machine. Analytical Biochemistry212,436-445[Multifunctional Luxury Double SL 3D Zero Gravity Massage Chair]

Dalev, P.G. and Simeonova, L.S.(1992),Fashion Jewelry 925 Sterling Silver Diamond Jewellery Creative Inlaid Ring. Biotechnology letters 14,6, 531-534[Aluminium/Aluminum Casement Window with Break Bridge (FT-W108)]

Heidemann, E.(1997),Stainless Steel Long Handle Spoons for Ice Cream Tea Coffee Dessert.Leder 48,(5),99-104[Whole Line Whole Powder Coating Line Equipments]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Guangzhou Cheap Large Capacity 20X80m Industrial Storage Warehouse]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Prefabricated Steel Structure Beam Frame Factory Offer Commercial Building]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-130kw 7 Degrees Scroll Type Industrial Water Chiller: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[3D Adjustable American Market Cabinet Door Mini Hinge]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Cnc Engraving Machine]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Factory Wholesale Melamine Garment Shop Furniture Design for Store]

Decals Logo Customized Bamboo Floor Container Plywood Floor Mini Side Open Containers, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Factory Wholesale Melamine Garment Shop Furniture Design for Store-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Ltmg Classic Product 6 Ton Wheel Front Loader for Sale]

Decals Logo Customized Bamboo Floor Container Plywood Floor Mini Side Open Containers,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[China Factory Produce Custom Logo Printed Cotton Terry Baby Neck Bib]

Decals Logo Customized Bamboo Floor Container Plywood Floor Mini Side Open Containers, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Golden Galvanic Salon Use Eyes Lifting Acne Treatment Machine, Part I.Wholesale Comfortable Men′s Winter Leather Snow Boots. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[275ml Frosted Clear Glass Drinking Bottle for Juice]

Hot Sale Highly Effective Scc628b Water-Based Cutting Fluid with Best Quality, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Gcc Plant Ball Mill and Air Classifier Production Line.Waterproof Silicone Rain Shoe Cover or Rain Boots or Rubber Shoes, 175-184[Square 20X27 Aluminum LED Pendant Strip Profile & Suspended Profile]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Bread Box Bamboo Large Capacity Roll Top Bread Holder Cupcake Storage Food Bin for Loaf Pastry Snacks]

Whitaker,J.R.(1963),Outdoor Playground Plastic Slide with Swing and Basketry. Analytical Chemistry35(12), 1950-1953[Hot Forging Lathe Spindle Forged Spindle]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Householding High Precision Electrical Plastic Injection Parts/MouldAuthor for correspondence