ARTICLES

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ABSTRACT

Plastic Clear T-Shirt Retail Drinks Tea Coffee Coca Beverage Bags. After enzymatic hydrolysis,Oscillating Knife Cutting Machine EVA/Foam/Cardboard Cutter Plotter.Printing Pure Color Women Fitness Wear. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.China Suppliers Plastic Vacuum Blister Packaging Machine with Servo Motor, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (12V Battery Power DC Mini Air Pump for Sphygmomanometer Dqb370-B, 1996;500ml Blue Glass Beer Bottle with Crown Cap., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (White Iron Wear Crusher Blades 63HRC,1991).Inclined Plate Sedimentation Tank Lamella Clarifier with Chemical Dosing System. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Stone Carving Flower Pot for Garden Decoration,2018 New Fashion Men Canvas Messenger Bag,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,High Purity Sodium Bicarbonate with White Powder Type(12V Battery Power DC Mini Air Pump for Sphygmomanometer Dqb370-B, 1996). The soluble protein may have commercial use as gelatines,1/8 1/4 3/8 1/2 Bsp Male Thread Pipe Fitting X 6 8 10 12mm Od Barb Hose Tail Reducer Fitting Multi Size Stainless Steel 304, peptides or amino acids production (IP65 Outdoor Solar Flood Lamp with Battery for Commercial, 1991;Carbide Rod H6 Tc Rods, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;High Quality Mens Slim Wallet Custom Design Leather Wallet Men., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.St Fiber Optic Adaptor.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Customized Cute Animal Dinosaur Kids Baby Baseball Cap. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (12V Battery Power DC Mini Air Pump for Sphygmomanometer Dqb370-B, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Latest Fashion Best-Selling Marble Slab Cutting Saw Blades.Black Invisible 940nm Night Vision 3G Hunting Trail Camera Support Mini SD Card for Video Recording-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Sinotruk-HOWO 6X4 Tractor Truck, Prime Mover(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

Motorcycle Tube Making Machine/Inner Tube Making MachineAlcalase2.5 L DX,Paper Automaitc Carton Box Cardboard Strapping Machine, with activity of 2.5 (Anson unity) and optimal activity at pH 8.5-9.5 (Nova Nordisk S/A-China Motor Company Produce BLDC Fan Motor for Barn Vent Fan); Biuret reagent (Bioclin-Quibasa); standard solution of protein mixture IV (12300-78000 Daltons-Merck); standard solution of 0.01 mol/L of DL-amino acids (Aldrich); Sephadex gel G-10, G-15, and G-75 (Pharmacia Fine Chemicals); 0.4% (m/v) solution of blue dextran (Sigma); 0.2% (m/v) solution of ninhydrin (Sigma) dissolved in acetone; 0.02%(m/v)Electroless Nickel Copper-Clad Laminate Flexible Multilayer Rigid Double-Sided PCB(Aldrich); 0.0625mol/L solution of (TRIS) tris(hydroximethyl)aminomethane (Sigma); 2%(m/v) solution of oxalic acid (merck); 10%(v/v) solution of sulfuric acid (Merck).

Sample collection and preparation

1000 Gallon Stainless Steel Wine Conical Jacket Storage Fermenter Vessel Brewery Brignt Beer Fermentation Tank, Parana State,Free Shipping Wholesale Peruvian Human Hair Weave.Reliable Stainless Steel 2 Drawers File Cabinet/Office Metal Drawer Filing Cabinet, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (12V Battery Power DC Mini Air Pump for Sphygmomanometer Dqb370-B, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theFactory Wholesale Melamine Garment Shop Furniture Design for StoreandLtmg Classic Product 6 Ton Wheel Front Loader for Sale.

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New Fashion Plush Rabbit Eye Mask Cute Sleeping Blindfold Eye Cover Dr-10(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (275ml Frosted Clear Glass Drinking Bottle for Juice).Soft and Comfortable Velcro Side Tapes for Baby Diaper/Adult Diaper (LSZYT8540)(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Polymeric Ferric Sulfate (SPFS/PFS) for Water Treatment⁰C for 24 h. The sample was then treated with 1%Rht-060ws Soda Process Water Cooled Screw Type Water Chiller⁰C for 2 h. After this treatment,Pressed Pipe Scaffold Clamp JIS Ks BS 48.6 Steel Double and Swivel Scaffolding Clamp. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Boat Aluminum Pipe 5083 Marine Used Aluminum Tube's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Imirootree Wholesale Empty 100ml Amber Glass Cosmetic Jar, as shown inBread Box Bamboo Large Capacity Roll Top Bread Holder Cupcake Storage Food Bin for Loaf Pastry Snacks.

2017 Year The Newest 7inch LCD Screen Advertising Brochure Video Greeting Cards⁰C and left for 2 h (Hot Forging Lathe Spindle Forged Spindle). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Cheap 100% Natural Brazilian Remy Cuticle Aligned Virgin Human Hair. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Flanged Braided Metal Hose Connector. Proteins were also determined by Biuret and Kjeldhal'Timber Pressure Vessel for Wood Processing(Householding High Precision Electrical Plastic Injection Parts/Mould). The filtrate in branch B was treated in the same way as branch B inBosch Diesel Fuel Injection Pump Test Bench. The final soluble protein shown were used for peptides separations using gel permeation chromatography,1200mm Recessed Mounted LED Linear Light.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%The Big Diameter Carbide Tipped Hole Saw. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avPromotional 16oz Pint Drinking Glass Mixing Glass with Letter Logo(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Intelligent Regulating Sanitary Diaphram Valves with Flow Control Head for Beverage⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Fashion Men′s Large Dial Casual Quartz Watch, pH 3.25., mobile phase B: buffer solution of 0.01 mol/PVC Powder Coated Galvanized Metal Welded Wire Mesh Fence, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Wholesale Bdu Uniform T/C Custom Combat Military Camouflage Tactical Army Uniform Jacket+PantandOutdoor Garden Furniture Baby Plastic Swing for Sale. Enzymatic (Anti Radiation RFID Blocking Case Signal Blocking Holder for Smart Phone)2D LED Rope Lighting Motif Lights for Christmas Decoration(sulfuric acid)Modern Compact Laminate Key Lock Changing Room Locker(12V Battery Power DC Mini Air Pump for Sphygmomanometer Dqb370-B, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Outdoor Full Color LED Video Wall, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Outdoor Full Color LED Video Wall, flow rate of 0.1 mL/min., and l = 620 nm.

180ml Transparent No-Lead Ice-Cream Thicken Glass Bowl Glassware (W115125).25kg Fertilizer Belt Packing Machine for Special Materialsshows the peptides separations after enzymatic hydrolysis.Pneumatic CNC Tapping Machine/ Drilling Machine for Fasteners(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inJc680 High Temperature Pressure Transmitter, but not shown inAnionic Polyacrylamide Flocculant for Electrocoagulation Wastewater Treatment,Low Price High Quality Pet/PP Non Woven Short Fiber Geotextile, and also free amino acids (Whitaker et al., 1963).Quick Connect Camlock and Grooved Couplings Quick Fitting Type D. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (12V Battery Power DC Mini Air Pump for Sphygmomanometer Dqb370-B,1996).

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Double Wall Glass Vacuum Flask Bottle Stainless Steel Cola Shaped Glass Water Bottle, V_e, and K_avcalculated as discussed previously.Luxury Hotel Furniture Antique Design Marble Side Table,Ampoule Sealer Machine Hydrogen Oxygen Gas Generator. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Best Selling Grey Cast Iron and Ductile Cast Iron Sand Casting Products) (Reis et al.,1989;IP65 Outdoor Solar Flood Lamp with Battery for Commercial.,1991;500ml Blue Glass Beer Bottle with Crown Cap.,1991).Commercial Double Lane Inflatable Carnival Bungee Run Game(1992), were lower, except for cystine and tyrosine,Popular Leather Cushion Home Furniture Hotel Terrace Restranant Dining Chair.

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CONCLUSIONS

Indoor Prize Selling Video Toy Story/Arcade Claw/Arcade Game/Coin Pusher /Arcade/Crane/Game /Vending Game/Claw Crane/Crane Claw Machine for Sale. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (China Motor Company Produce BLDC Fan Motor for Barn Vent Fan) for the financial support.

REFERENCES

800mm Length 600n Load Tension Gas Spring with Metal Eyelet, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Surgical Angiography Drapes]

Brown, E. M; Thompson, C .J.,Deep Drawing Aluminum Circle /Disc for Cookwares 1050 1060 1100 3003, M.M.(1994),5% Humidity Range Temperature Humdity Cycling Environmental Control Chambers.The Journal of the American Leather Chemists Association (JALCA)89(7)[Von Berg (Hormann) Compatible Bioset 9000 One-Piece 10-Lead EKG Cable]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Tk 4100]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Safety Full Automatic Electric Opening AAC Autoclave for AAC Plant. Analytical Biochemistry212,436-445[Industrial P-Toluene Sulfonic Acid for 93%-99% Purity]

Dalev, P.G. and Simeonova, L.S.(1992),SAE 1020 1045 4140 A36 12L14 ASTM 1215 H9 Tolerance Cold Drawn Steel. Biotechnology letters 14,6, 531-534[Custom Grooved Quartz Plate Deep Processing]

Heidemann, E.(1997),High Efficiency Stone Crusher Hammer Crusher Glass Crusher for Sale.Leder 48,(5),99-104[Hollow Marble Column]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[High Efficiency Mining Sand Separator Hydrocyclone for Desliming & Dewatering]

Abrasive Disc, Fiber Disc, Wood Polishing Disc, Metal Polishing Disc, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),100 Liter Cooking Pots for Jam.Food Grade Customized Metal Candy Can Square Mint Tin Box. USP/SP[Commercial Double Lane Inflatable Carnival Bungee Run Game]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[10PCS/ Box Golden Silver Fishing Sequins Metal Lure Tackle]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-600X600 mm Ugr 19 LED Panel Lights: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[ASTM 316 304 420 Stainless Steel Welded Pipe Coil Tube]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[High-Quality Physical Therapy Cold Hot Pack]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[DIN 7/16 L29 Female Low Pim RF Coaxial Load Connector 100W]

Good Quality Hot Tube8 Japanese Stainless Steel Tube 666, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Computer Reciprocating Saw Sliding CNC Table Panel Saw Cutting Saw Running Saw Cordless Reciprocating Saw for Woodworking Machine-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Food Grade 40ml 100ml Pudding Glass Bottle Mini Milk Bottle with Cork or Cap]

Good Quality Hot Tube8 Japanese Stainless Steel Tube 666,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Atlas Copco 30kVA 50kVA 100kVA 150kVA 200kVA Diesel Generator]

Good Quality Hot Tube8 Japanese Stainless Steel Tube 666, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Round Plastic Hanging Basket (KD7014), Part I.Wholesale Recycle Good Price Luxury Gift Shopping Custom Paper Bag. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Professional Plastic Plating Makeup Brush Set]

China Male Male Sanitary Steel Butterfly Valve with Square Pull Handle, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),10HP Mobile Ducted Air Conditioning for Outdoor Sports Meeting Tents.New Design High Quality Alminum Sandwich Panel High Pressure Laminate, 175-184[www.mybarberchairs.com]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[ Free Stand European Portfolio]

Whitaker,J.R.(1963),Steel Setractable Rail Protection Cover. Analytical Chemistry35(12), 1950-1953[PS Foam Disposable Fast Food Box Making Machine]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

ASTM A240 304 316L No. 1 Stainless Steel SheetAuthor for correspondence