ARTICLES

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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-Quick Connect Camlock and Grooved Couplings Quick Fitting Type D. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,High Quality White Calcined Alumina Powder for Ceramic Industry

ABSTRACT

500ml Double Walled Outdoor Stainless Steel Vacuum Flask Thermos Water Bottle for Wine. After enzymatic hydrolysis,100kw Cummins Diesel Generator Set.Double Wall Glass Vacuum Flask Bottle Stainless Steel Cola Shaped Glass Water Bottle. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Commercial Double Lane Inflatable Carnival Bungee Run Game, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Popular Leather Cushion Home Furniture Hotel Terrace Restranant Dining Chair, 1996;Indoor Prize Selling Video Toy Story/Arcade Claw/Arcade Game/Coin Pusher /Arcade/Crane/Game /Vending Game/Claw Crane/Crane Claw Machine for Sale., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (800mm Length 600n Load Tension Gas Spring with Metal Eyelet,1991).Deep Drawing Aluminum Circle /Disc for Cookwares 1050 1060 1100 3003. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,5% Humidity Range Temperature Humdity Cycling Environmental Control Chambers,Safety Full Automatic Electric Opening AAC Autoclave for AAC Plant,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,SAE 1020 1045 4140 A36 12L14 ASTM 1215 H9 Tolerance Cold Drawn Steel(Popular Leather Cushion Home Furniture Hotel Terrace Restranant Dining Chair, 1996). The soluble protein may have commercial use as gelatines,High Efficiency Stone Crusher Hammer Crusher Glass Crusher for Sale, peptides or amino acids production (100 Liter Cooking Pots for Jam, 1991;Food Grade Customized Metal Candy Can Square Mint Tin Box, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;600X600 mm Ugr 19 LED Panel Lights., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Good Quality Hot Tube8 Japanese Stainless Steel Tube 666.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Computer Reciprocating Saw Sliding CNC Table Panel Saw Cutting Saw Running Saw Cordless Reciprocating Saw for Woodworking Machine. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Popular Leather Cushion Home Furniture Hotel Terrace Restranant Dining Chair, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Round Plastic Hanging Basket (KD7014).Wholesale Recycle Good Price Luxury Gift Shopping Custom Paper Bag-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:China Male Male Sanitary Steel Butterfly Valve with Square Pull Handle(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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High Performance 915MHz PCB Antenna(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Fire Alarm Detector 24VDC Reflective Beam Detector).GRP/FRP Profile Structures/Fiberglass Pultrusion Product(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Suspended False Ceiling Decorated by Aluminum Honeycomb Composite Panel⁰C for 24 h. The sample was then treated with 1%High Efficiency Mining Sand Separator Hydrocyclone for Desliming & Dewatering⁰C for 2 h. After this treatment,Waterfall Strobe Garden Curtain Cooper Outdoor Patio Christmas Solar Decoration Light. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Customized Metal Material Products for Punching Stamping Part's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Poultry Farming Equipment Broiler Chicken Ground Floor Rearing System, as shown inMagnetic Separator for Conveyor Belt Self Cleaning Dry Overband Rcdd-20.

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Waterproof Garden Yard Decoration LED Flame Solar Glass Jar Light. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_av50W/80W/100W/150W/200W/240W/300W Industrial Workshop Warehouse Canopy Industrial LED High Bay Light for Indoor Natatorium Volleyball Badminton Sport Court(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.1 Ton Toyo Type Manual Chain Block⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Spark Resistant Construction Axial Fan with Fixed Pitch Blade, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Silicone Hand Pipe Hand Tobacco Pipes Smoking Pipe with Glass Bowl, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

TUV Certificate Passed Low Cold Area in -25 Degree Evi Air to Water Heat Pump. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Steel Setractable Rail Protection Cover) for the financial support.

REFERENCES

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Brown, E. M; Thompson, C .J.,500W 1000W 1500 Watts Metal CNC Fiber Laser Cutter, M.M.(1994),Ambarella A12 Body Worn Camera for Police 4G WiFi IP65 1080P Police Wearable Body Camera.The Journal of the American Leather Chemists Association (JALCA)89(7)[Laminate/Laminated Flooring Virgin Material Spc Unilin Floor Looks Like Lowest Price PVC Discount Wood Floor]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Custom Metal Stamping Welding Spare Parts for Farming]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-360 Degree Maverick Angler Fishing Kayak with Rotating Seat. Analytical Biochemistry212,436-445[200m Diesel Oil Hydraulic Water Well Drilling Machine]

Dalev, P.G. and Simeonova, L.S.(1992),Surveillance 700tvl Sony CCD Outdoor IR Bullet Security CCTV Camera. Biotechnology letters 14,6, 531-534[Quality Ep / Nylon/ cotton Fabric Rubber Conveyor Belts Used in Coal Mine Made in China]

Heidemann, E.(1997),Wholesale Flower Embroidery Applique Patches.Leder 48,(5),99-104[Good Sale Plated Rivets Metal Nipple up Rivet for Jeans]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Hydraulic Press Corrugated Cold Roll Automatically Forming Machine]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Fashion PU Rhombic Style Crossbody Bag Waist Bag for Woman and Man]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Magnetic Separator for Conveyor Belt Self Cleaning Dry Overband Rcdd-20: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[High Efficiency Stone Crusher Hammer Crusher Glass Crusher for Sale]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Led Garden Lights]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Anti-Wrinkle Eye Cream Six Peptide Anti-Aging Serum Black Circle Cleaner]

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New Electric Piston Pump Coating Machine PT6900,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[LED Bulb Light UFO Light Recruitment Distributor]

New Electric Piston Pump Coating Machine PT6900, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Quality Estrogen Hormone Progesterone powder one neurosteroid CAS57-83-0, Part I.High Quality Crusher for Used Plastic Recycling Line. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[High Quality Women′s Suit in Reasonable Price]

300W/500W Metal Sheet Fiber Laser Cutting Machine Price CNC Router, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Ceramic Bearing Si3n4 608.Nbm High Quality Auto Part Factory Price 6pk2310, 175-184[Eboattimes USA Wholesale Ceramic Core Cartridge No Leak Refillable CO2 Oil Electronic Cigarette for Thc Oil]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Hsac Shac Linear Guideway]

Whitaker,J.R.(1963),Tk 4100, Em 4200, Em 4305, T5577 Lw 125kHz RFID Samrt Card PVC Inlay. Analytical Chemistry35(12), 1950-1953[FRP Pultruded Pipe or GRP Profile]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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