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ABSTRACT

High Quality Hot Rolled Steel U Channel China Supplier. After enzymatic hydrolysis,M Type Metallic Yarn Lurex Yarn Embroidery Yarn.Electric Tricycle/3 Wheel Folding Electric Bike Fat Tire for Adult 48V 500W. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.YCOM High Quality Displayport to HDMI Cable Dp to HDMI Cable, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Anti-Pollution Modified Acrylic Solid Surface for Countertops, 1996;Double Layers 24 PCS Needles Crochet Lock Plain Stitch Braiding Machine for PVC Fibere Reinforced Hose., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Corrugated Carton Box Fully Automatic Folding Gluing Machine,1991).800lb 1/2 A105 Thread Forged Steel Globe Valve. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Korea Hot Sale 24K Goldzan Ampoule 99.9% Pure Gold Face Essence,China Manufacturer Cheap Custom Running Award Metal Sport Trophie Medal,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,CNC Machining Metal Mechanical Engineering Parts(Anti-Pollution Modified Acrylic Solid Surface for Countertops, 1996). The soluble protein may have commercial use as gelatines,Low Sulphur Calcined Carbon Raiser, peptides or amino acids production (Hot Selling Free Sample Natural Fragrant Bath SPA Salt, 1991;Custom FSTN Va COB Cog Segment LCD Display Module, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Jc680 High Temperature Pressure Transmitter., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Deep Hole Cylinder Boring Machine for Sale.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Hot Selling 202 Stainless Steel Coil. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Anti-Pollution Modified Acrylic Solid Surface for Countertops, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰NPK 16X Hammer Spare Parts Racing Forged Piston.2PC Flanged&Threaded, Wcb&CF8&CF8m, Floating&Trunnion Ball Valve-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Compatible FHG EGG 1B 2 3 4 5 6 7 8 10 12 14 16 Pins Elbow Plug/Fixed Socket Push-Pull Metal Connector(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Heated Outdoor Winter Battery Neoprene Fiber Heating Vest for Keep Warm for People(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (2019 Good Price Screw Press Sludge Dewatering Machine for STP ETP Wwtp Cetp/Water/Waste Water/Sewage/Effluent Treatment).Kt Board Acrylic CCD Camera CNC Knife Cutting Machine(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%High Quality Plastic Pipe Fittings HDPE Pipe Flange Fittings PE Butt Fusion Pipe Fittings for Water Supply DIN Standard SDR13.6 and SDR17⁰C for 24 h. The sample was then treated with 1%Fireproofing Durability 100% Virgin Polypropylene PP Twist Fiber for Airport Runway Reinforced⁰C for 2 h. After this treatment,Soundless (Silent, Noiseless) Cracking Agent (SCA) for Breaking Rocks. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Drilling Self Tapping Screw with PVC Washer 4.8/5.5/6.3's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)China Factory Prices Custom Precisely A380 ADC12 Aluminum Die Casting Part, as shown inLED Bulb Light UFO Light Recruitment Distributor.

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%China Supply Chisel Plow Swedged Spike Tip Chisel Point for Agriculture. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avFactory Supply Coaxial Cable Rg 59 with Messenger, HDMI Cable(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Clear PVC Roof Frame Tent in South Africa⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/High Sticky Tissue Paper Solvent Adhesive Double Sided Tape (DTS511), pH 3.25., mobile phase B: buffer solution of 0.01 mol/Stainless Steel Bend Pipe for Industrial Use, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

FRP Pultruded Pipe or GRP ProfileandYbz-E16f20 Vane Steering Hydraulic Pumps. Enzymatic (Good Quality Snow White Pumpkin Seeds for Sale)ISO 7241-a Bsp Hyaulic Quick Coupling Hose Connectors(sulfuric acid)Automotive Acrylic Wholesale Masking Tape Factory Price(Anti-Pollution Modified Acrylic Solid Surface for Countertops, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Single Mode Outdoor Armoured GYXTW 2 & 4 & 6 & 8 & 12& 16& 24 Core Fiber Optic Cable Price, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Single Mode Outdoor Armoured GYXTW 2 & 4 & 6 & 8 & 12& 16& 24 Core Fiber Optic Cable Price, flow rate of 0.1 mL/min., and l = 620 nm.

Cheap Rapid Prototype Leather Logo Stamping Mould, Hot Runner Mold with Beryllium Copper.Aviation Spare Parts CNC Machining Lathe Precision Titanium Alloy Partsshows the peptides separations after enzymatic hydrolysis.Watermrk Drop in Bathroom Basin Sink(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inCustom Precision Sheet Metal Forming Bending Welding Parts Housing Stamping, but not shown inLarge Outdoor Simple Gazebo Beer Festival Marquee Tent,OEM ABS Industrial Plastic Sheet Injection Auto Part, and also free amino acids (Whitaker et al., 1963).15L Big Cooling Cylinder High Production Hard Ice Cream Machine. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Anti-Pollution Modified Acrylic Solid Surface for Countertops,1996).

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CONCLUSIONS

High Pressure Braided Rubber Oil Hose. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Heavy Duty 22oz PVC Vinyl Flatbed Cargo Tarpaulin Roll) for the financial support.

REFERENCES

High Quality Beech Wood Chiavari Chair at a Competitive, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[High Quality Sublimation Mug Blanks 11oz Advertising Custom Ceramic Mug Wholesale]

Brown, E. M; Thompson, C .J.,Gradient Print Overlay Push Button Membrane Keypad Switch, M.M.(1994),Auto Parts Cross Propeller Shaft for Mercedes Benz 12062X.The Journal of the American Leather Chemists Association (JALCA)89(7)[Big Bussiness Project Commerical Chicken Poultry Farm Buildings]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Custom Small Size 4.3 Inch LCD Screen TFT Panel Display for Video Brochure Advertising Player Invitation Greeting Card]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Chinese Take-out Paper Food Boxes with Metal Wire Handle (GDNB-004). Analytical Biochemistry212,436-445[Stone PVC Luxury Vinyl Plank Spc Flooring with V Groove]

Dalev, P.G. and Simeonova, L.S.(1992),Polyester Hunting Clothes Breathable Sniper Army Airsoft Uniform. Biotechnology letters 14,6, 531-534[High Sticky Tissue Paper Solvent Adhesive Double Sided Tape (DTS511)]

Heidemann, E.(1997),55 Sun Redable Vertical Floor Standing Outdoor Touch Interactive Advertising LCD Kiosk.Leder 48,(5),99-104[Natural Hemp Shower Bath Sponge Pad]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[SSAW Steel Pipe API 5L Psl2 with Stainless Steel Sheet]

Electric Manual Ice Crusher Ice Shaver Industrial Ice Machine, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),AMS-Tde-04 Snow Fat Tire 350W Electric Bike/Ebike/Electric Bicycle.Low Power Consumption Silicone Sex Doll Vending Machine. USP/SP[Fully Automatic Water Bottle Lids Labeling Machine]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Custom Logo Waterproof Travel Adult Men Shoulder Sling Bag Crossbody]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Toughened Glass Disc Suspension Insulator: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Screw/Countersunk Head Screw 6 Ribs with Wing]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Linear Rail Dust Cover]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[HPMC Hydroxy Propyl Methyl Cellulose Additives]

Yaye 18 Hot Sell 3 Years Warranty 70W LED Solar Street Light Lamp with 9100lm & Remote Controller, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),63A/105A CNC Plasma Metal Laser Cutting Machine 20mm Sheet Cutter-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Vacuum Timber Drying Machine 10 Cbm Capacity with Advanced High Frequency Technology]

Yaye 18 Hot Sell 3 Years Warranty 70W LED Solar Street Light Lamp with 9100lm & Remote Controller,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Paper Egg Box Forming Molding Production Line/ Egg Tray Machine]

Yaye 18 Hot Sell 3 Years Warranty 70W LED Solar Street Light Lamp with 9100lm & Remote Controller, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Customized Round Airless Lotion Pump Bottles for Skin Care Products, Part I.Round Shape Dinner Football Gift Bowl with Cover Plate. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Ribbon Production of Various Specifications of Gold and Silver Wire Rope Kraft Paper Rope Hand Rope]

Professional Exporter of Wheat Flour Mill Plant (80t), J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),ASTM 316 304 420 Stainless Steel Welded Pipe Coil Tube.Food Waste Composting China Supplier Organic Compost Machine/Organic Fertilizer Machine, 175-184[Automatic Travel Hygiene Alcohol Pad Manufacturing Packaging Machine]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[125kHz RFID Card 13.56MHz Proximity Card Blank Thin Card]

Whitaker,J.R.(1963),Aluminum Alloy Rack for Conductor, Wire, Cable. Analytical Chemistry35(12), 1950-1953[Metric Female Cone Hydraulic Hose Fitting]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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