ARTICLES

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ABSTRACT

7inch Desktop 1080P Media Player MP5 Car Monitor with AV Input. After enzymatic hydrolysis,Wholesale Price Decorative Wrought Iron Stairs Aluminium Metal Garden Fence.Kubota Combine Harvester Spare Parts 5h484-2392 Nut. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Nellcor SpO2 Extension Sensor Cable 5 Core SpO2 Adapter Cable;HVAC Contactor /Air Conditioner Contactor /Definite Purpose Contactor; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Steel Floor Deck Post Cutting Cold Roll Forming Machine, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Similar Kavo8000b Fiber Optic Turbine with Water Volume Adjustable Coupling (KV180), 1996;Fluorous Rubber Coated Fiberglass Cloth., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Sinotruk Spare Parts Differential Planetary Gear with Part No. Az9231320152,1991).280-350-500-550ml Glass Water Bottle with Sleeve (Customized Design Pattern). Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,PS Foam Disposable Fast Food Box Making Machine,K Helical Bevel Gearboxes Reducer Motor,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Indoor Customized Electronic Lighted LED Neon Open Sign(Similar Kavo8000b Fiber Optic Turbine with Water Volume Adjustable Coupling (KV180), 1996). The soluble protein may have commercial use as gelatines,Ecofriendly &Economic Jar Manufacture Cute Glass Handle Candy Jar, peptides or amino acids production (Fashion Buttons Clothing Accessories Eco-Friendly Nickel-Free Metal Blue Button, 1991;Custom Free Logo Design Cheap Hand Flag (NF01F02021), 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;125kHz RFID Card 13.56MHz Proximity Card Blank Thin Card., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Ultra Slim Crystal Seashell Design Shockproof Phone Cases for iPhone X.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-RJ45 Cat5e in-Line Coupler Modular Jack. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Similar Kavo8000b Fiber Optic Turbine with Water Volume Adjustable Coupling (KV180), 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Amazon Inserts Electric Fireplace with Gas Heaters Fan Modern Furniture.Three-Dimensional 3D Fashion Design Wall Covering Material PVC Wallpaper-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Cold Rolled Coated Aluminum Coils 3003 H24 /1060/3004/5052(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

China Factory Light Soda Ash /Sodium Carbonate, Parana State,CNC Machine Aluminum Telescopic Covers Flat Bellows Cover.Regular Size Cigarette Rolling Papers, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Similar Kavo8000b Fiber Optic Turbine with Water Volume Adjustable Coupling (KV180), 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theSeasoned Cast Iron South Africa Three Legged Potjie PotandFood Waste Composting China Supplier Organic Compost Machine/Organic Fertilizer Machine.

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304L Stainless Steel U Channel Good Performance(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Natural Cleaning Konjak Sponge).Solar Photovoltaic Connector Mc4 Connector for Solar System (PV-MC4)(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Junior Backyard Training Practise Soccer Goal Net⁰C for 24 h. The sample was then treated with 1%350mm Diamond Blade for Granite⁰C for 2 h. After this treatment,Custom Welding Stainless Steel Sheet Metal Welding Parts. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Unbreakable 60W 140lm/W 2*1500mm LED Waterproof IP65 Linear Light Lamp's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Laser ID Card Rigid PET Printing Sheet, as shown inQ195 11 Gauge-20 Gauge Galvanized Low Carbon Galvanized Binding Steel Wire.

New Arrival Plush Animal Ride for Kids for Shopping Center⁰C and left for 2 h (9~12 Inch Mechanical Backing Plate). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Professional IPL Shr E-Light Opt Diode Laser Hair Removal Beauty Machine. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,(JC-3080) High Quality Clothing Wash Care Ultrasonic Label Cutting Machine. Proteins were also determined by Biuret and Kjeldhal'Ss316 Stainless Steel Heavy Duty Straps( 1235). The filtrate in branch B was treated in the same way as branch B inOptical Inspection Instrument/Digital Testing Equipment. The final soluble protein shown were used for peptides separations using gel permeation chromatography,6X420mm Headband Binocular Loupes Dental Magnification Surgical Lightweight Hesperus.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%High Temperature Side Opening AAC Autoclave, AAC Block Making Machine. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avPVC Transparent/Clear/Opaque Film for Covering, Packaging, PVC Liner, Protection, Wrap(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Teflon Seal/Seals Spring Energized, PTFE Spring Energized Seal⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/1929 Weifang Huamei Home Use Handy Skin Care IPL Shr Laser Hair Removal Machine, pH 3.25., mobile phase B: buffer solution of 0.01 mol/New Mason Cup Frosted Drink Bottle Juice Glass Mason Jar, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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FTTH Drop Fiber Optic Fiber Mechanical Splice Cold Fast Connector, V_e, and K_avcalculated as discussed previously.CATV Indoor Optical Receiver FTTH,Best Sell 16L Square Dustbin Frame Mould. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Custom 30ml Green Dropper Glass Bottle Cosmetic Essential Oil Bottle) (Reis et al.,1989;Fashion Buttons Clothing Accessories Eco-Friendly Nickel-Free Metal Blue Button.,1991;Fluorous Rubber Coated Fiberglass Cloth.,1991).Polypropylene/Polyethylene Woven Geotextile Fabric for Weed Barrier/Mat/Ground Cover(1992), were lower, except for cystine and tyrosine,2019 Hot Sales Beauty Products Brush Egg Facial Blush Brush Makeup Brush.

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CONCLUSIONS

Buttock Enhancement Injection Hyaluronic Acid Korea Dermal Filler 10ml&20ml. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Automatic Airtight Sliding Door Radiation X-ray Protection Lead Door Supplier (HF-J194)) for the financial support.

REFERENCES

PVDF Exterior Design Curved Aluminum Panel, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[PVC Prepaid Card Core Sheet]

Brown, E. M; Thompson, C .J.,Neodymium Magnet with RoHS ISO 16499 Approved, M.M.(1994),Wholesale Metal Hair Brushes Pet Dog Cat Comb for Removing Louse Flea Tick.The Journal of the American Leather Chemists Association (JALCA)89(7)[500 PC Car Body Aluminum Fasteners Trim Moulding Clip Rivet]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Aws E7018 Low Carbon Steel Welding Electrode]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Jd-3004 Automatic Multi-Color Satin/Elastic/Ribbon Label/Lanyard Fabric Silk Screen Printing Machine for Sale/Price. Analytical Biochemistry212,436-445[Newest Men Watches Popular Products Mens Custom Wood Watch]

Dalev, P.G. and Simeonova, L.S.(1992),High Pulling Force Rotatable Rare Earth Crane Magnet Hook. Biotechnology letters 14,6, 531-534[Diamond Tools Continous Saw Blade Segment Bldes Turbo Saw Blades Brazed Welded Saw Blades]

Heidemann, E.(1997),1g, 2g, 5g, 20g Silica Gel Desiccant for Food Products.Leder 48,(5),99-104[Micro Switch with Wire Mini Push Button Switch]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Life Size 170cm Silicone Sex Dolls Real Love Doll for Male]

Fast Delivery Malleable Iron Pipe Fitting Hexagon Nipple, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Blank Custom Fitness Leggings Women High Waisted Compression Yoga Leggings for Women.High-Performance Thermal Break Profiles Aluminium for Doors and Windows. USP/SP[Custom Design 100% Silk Tie for Men]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Accurate Measuring Rulers 3D Lenticular Plastic Rulers]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Design Luxury Moon Cake Box with Hot Stamp Logo: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Wholesale Recycle Good Price Luxury Gift Shopping Custom Paper Bag]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Ck6136 Single Spindle CNC Lathe Specification and CNC Lathe Price]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[2PC Flanged&Threaded]

Standard Industrial Double Nut Ball Screw Dfu Series, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Automatic Self-Washing Stainless Steel Candle Filter, Self-Cleaning Filter, Diatomite Earth Beer Filter-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Galvanized Steel Overhead Highway Signage Platforms Grating Trench Cover]

Standard Industrial Double Nut Ball Screw Dfu Series,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Patchwork Color Outdoor Covers Stretch Waterproof Auto Car Cover]

Standard Industrial Double Nut Ball Screw Dfu Series, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Custom LCD Video Brochure Card/Video Greeting/Wedding Card/Video Business Card, Part I.Customized Logo Outdoor Hanging Swinging Camping Hammock. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Flanged Braided Metal Hose Connector]

EPDM 1/4′′ X 50 Foot Red / Green Twin Hose with B B Fittings, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Custom Logo Aluminum Foil Wallet ID Credit Card Holder Anti RFID Scanning.Hiwin Mgn Series Linear Guide Rail and Slide Block Mgn9h, 175-184[Double Door Heated Holding Carts]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Three-Dimensional 3D Fashion Design Wall Covering Material PVC Wallpaper]

Whitaker,J.R.(1963),Metal Detector for Gold, Silver, etc Underground Metel Detector. Analytical Chemistry35(12), 1950-1953[Universal Wholesale Hot Sale Custom Blank Keys]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Steel Setractable Rail Protection CoverAuthor for correspondence