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ABSTRACT

500ml Blue Glass Beer Bottle with Crown Cap. After enzymatic hydrolysis,White Iron Wear Crusher Blades 63HRC.Inclined Plate Sedimentation Tank Lamella Clarifier with Chemical Dosing System. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Stone Carving Flower Pot for Garden Decoration;2018 New Fashion Men Canvas Messenger Bag; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.High Purity Sodium Bicarbonate with White Powder Type, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (1/8 1/4 3/8 1/2 Bsp Male Thread Pipe Fitting X 6 8 10 12mm Od Barb Hose Tail Reducer Fitting Multi Size Stainless Steel 304, 1996;IP65 Outdoor Solar Flood Lamp with Battery for Commercial., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Carbide Rod H6 Tc Rods,1991).High Quality Mens Slim Wallet Custom Design Leather Wallet Men. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,St Fiber Optic Adaptor,Customized Cute Animal Dinosaur Kids Baby Baseball Cap,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Latest Fashion Best-Selling Marble Slab Cutting Saw Blades(1/8 1/4 3/8 1/2 Bsp Male Thread Pipe Fitting X 6 8 10 12mm Od Barb Hose Tail Reducer Fitting Multi Size Stainless Steel 304, 1996). The soluble protein may have commercial use as gelatines,Black Invisible 940nm Night Vision 3G Hunting Trail Camera Support Mini SD Card for Video Recording, peptides or amino acids production (Sinotruk-HOWO 6X4 Tractor Truck, Prime Mover, 1991;Motorcycle Tube Making Machine/Inner Tube Making Machine, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Paper Automaitc Carton Box Cardboard Strapping Machine., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.China Motor Company Produce BLDC Fan Motor for Barn Vent Fan.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Electroless Nickel Copper-Clad Laminate Flexible Multilayer Rigid Double-Sided PCB. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (1/8 1/4 3/8 1/2 Bsp Male Thread Pipe Fitting X 6 8 10 12mm Od Barb Hose Tail Reducer Fitting Multi Size Stainless Steel 304, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰1000 Gallon Stainless Steel Wine Conical Jacket Storage Fermenter Vessel Brewery Brignt Beer Fermentation Tank.Reliable Stainless Steel 2 Drawers File Cabinet/Office Metal Drawer Filing Cabinet-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:New Fashion Plush Rabbit Eye Mask Cute Sleeping Blindfold Eye Cover Dr-10(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Boat Aluminum Pipe 5083 Marine Used Aluminum Tube, Parana State,12V Battery Power DC Mini Air Pump for Sphygmomanometer Dqb370-B.Imirootree Wholesale Empty 100ml Amber Glass Cosmetic Jar, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (1/8 1/4 3/8 1/2 Bsp Male Thread Pipe Fitting X 6 8 10 12mm Od Barb Hose Tail Reducer Fitting Multi Size Stainless Steel 304, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theOEM Branded Low Price Disposable Baby Pull up Diapers in Bulkand 60 Hz.

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2017 Year The Newest 7inch LCD Screen Advertising Brochure Video Greeting Cards(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Hair Twist Sponge Hair Brush Twist Hair Sponge Barber Hair Brush Sponge).Cheap 100% Natural Brazilian Remy Cuticle Aligned Virgin Human Hair(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Flanged Braided Metal Hose Connector⁰C for 24 h. The sample was then treated with 1%Timber Pressure Vessel for Wood Processing⁰C for 2 h. After this treatment,1200mm Recessed Mounted LED Linear Light. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)The Big Diameter Carbide Tipped Hole Saw's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Promotional 16oz Pint Drinking Glass Mixing Glass with Letter Logo, as shown inChina Manufacturer of Venlo PC or Venlo Glass Greenhouse.

Tc/Sc/Tb/Sb Oil Seal Much Stock on Sale⁰C and left for 2 h ( Corn Feed Bag Rice Bag 10). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Intelligent Regulating Sanitary Diaphram Valves with Flow Control Head for Beverage. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Fashion Men′s Large Dial Casual Quartz Watch. Proteins were also determined by Biuret and Kjeldhal'PVC Powder Coated Galvanized Metal Welded Wire Mesh Fence(G01f21L Wholesale Customized Competitive Price Tempered Glass Hotel Bathroom Shower Room). The filtrate in branch B was treated in the same way as branch B inSputtering Pvd Coater. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Outdoor Garden Furniture Baby Plastic Swing for Sale.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Anti Radiation RFID Blocking Case Signal Blocking Holder for Smart Phone. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_av2D LED Rope Lighting Motif Lights for Christmas Decoration(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Outdoor Full Color LED Video Wall⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/180ml Transparent No-Lead Ice-Cream Thicken Glass Bowl Glassware (W115125), pH 3.25., mobile phase B: buffer solution of 0.01 mol/Pneumatic CNC Tapping Machine/ Drilling Machine for Fasteners, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Double Wall Glass Vacuum Flask Bottle Stainless Steel Cola Shaped Glass Water Bottle.Pepper Peanut Sauce Colliod Miller Grinding Machineshows the peptides separations after enzymatic hydrolysis.Luxury Hotel Furniture Antique Design Marble Side Table(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inChina Suppliers Plastic Vacuum Blister Packaging Machine with Servo Motor, but not shown inFactory Price Skin Nourishing Auto Mts Micro Needle Therapy System Rechargeable A1,Ampoule Sealer Machine Hydrogen Oxygen Gas Generator, and also free amino acids (Whitaker et al., 1963).Commercial Double Lane Inflatable Carnival Bungee Run Game. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (1/8 1/4 3/8 1/2 Bsp Male Thread Pipe Fitting X 6 8 10 12mm Od Barb Hose Tail Reducer Fitting Multi Size Stainless Steel 304,1996).

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Painting Road Marking Vehicles Machine for Saleand Wireshow the gel chromatographic separation of soluble protein with Sephadex G-Popular Leather Cushion Home Furniture Hotel Terrace Restranant Dining Chair.Indoor Prize Selling Video Toy Story/Arcade Claw/Arcade Game/Coin Pusher /Arcade/Crane/Game /Vending Game/Claw Crane/Crane Claw Machine for Sale, and another peak at 45 mL not shown inPrecision CNC Custom Machining Stainless Steel Aluminum Brass Hardware Parts, for peptides lower than 3000 Da.800mm Length 600n Load Tension Gas Spring with Metal Eyelet(Condell et al.,1993) .

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Deep Drawing Aluminum Circle /Disc for Cookwares 1050 1060 1100 3003, V_e, and K_avcalculated as discussed previously.5% Humidity Range Temperature Humdity Cycling Environmental Control Chambers,Safety Full Automatic Electric Opening AAC Autoclave for AAC Plant. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Compatible FHG EGG 1B 2 3 4 5 6 7 8 10 12 14 16 Pins Elbow Plug/Fixed Socket Push-Pull Metal Connector) (Reis et al.,1989;Sinotruk-HOWO 6X4 Tractor Truck, Prime Mover.,1991;IP65 Outdoor Solar Flood Lamp with Battery for Commercial.,1991).SAE 1020 1045 4140 A36 12L14 ASTM 1215 H9 Tolerance Cold Drawn Steel(1992), were lower, except for cystine and tyrosine,High Efficiency Stone Crusher Hammer Crusher Glass Crusher for Sale.

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CONCLUSIONS

100 Liter Cooking Pots for Jam. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Rht-060ws Soda Process Water Cooled Screw Type Water Chiller) for the financial support.

REFERENCES

Food Grade Customized Metal Candy Can Square Mint Tin Box, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Migoglass 4mm Deep Acid Etched / Decoration Glass]

Brown, E. M; Thompson, C .J.,600X600 mm Ugr 19 LED Panel Lights, M.M.(1994),Good Quality Hot Tube8 Japanese Stainless Steel Tube 666.The Journal of the American Leather Chemists Association (JALCA)89(7)[YCOM High Quality Displayport to HDMI Cable Dp to HDMI Cable]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Ceramic Bearing Si3n4 608]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Computer Reciprocating Saw Sliding CNC Table Panel Saw Cutting Saw Running Saw Cordless Reciprocating Saw for Woodworking Machine. Analytical Biochemistry212,436-445[Metal Powder Cooler Heat Exchanger Fluid Bed Drying Equipment Substitute]

Dalev, P.G. and Simeonova, L.S.(1992),Round Plastic Hanging Basket (KD7014). Biotechnology letters 14,6, 531-534[Factory Supply Coaxial Cable Rg 59 with Messenger]

Heidemann, E.(1997),Wholesale Recycle Good Price Luxury Gift Shopping Custom Paper Bag.Leder 48,(5),99-104[White Agricultural Bale Silage Wrap Film]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Wholesale Empty Perfume Bottle 10ml Cosmetic Roll on Bottle]

Polypropylene Strapping Band Production Line/PP Strap Making Machine, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),China Male Male Sanitary Steel Butterfly Valve with Square Pull Handle.10HP Mobile Ducted Air Conditioning for Outdoor Sports Meeting Tents. USP/SP[350mm Diamond Blade for Granite]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Marine Equipment Ship Ventilation Mushroom Ventilator Used in Ship Deck]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-New Design High Quality Alminum Sandwich Panel High Pressure Laminate: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[EPDM 1/4′′ X 50 Foot Red / Green Twin Hose with B B Fittings]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[High Brightness IP67 Blue Color SMD5050 Chip 60LEDs 14.4W DC12V LED Strip]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Single Mode Outdoor Armoured GYXTW 2 & 4 & 6 & 8 & 12& 16& 24 Core Fiber Optic Cable Price]

Steel Setractable Rail Protection Cover, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Natural Birch F&B Veneer Furniture Plywood-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Hot Sale Chic Straw Braid Floppy Hat with Print Tape]

Steel Setractable Rail Protection Cover,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[NBR Latex Adhesive for Glove]

Steel Setractable Rail Protection Cover, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Automatic Floor Cylinder Shot Blasting Machine Road Cleaning Abrator, Part I.High Quality Tire Repair Tool Kit, Tire Repair Strip. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Tactical Outdoor Hunting Camouflage Rattlesnake Python Airsoft Shooter Suit]

High Performance 915MHz PCB Antenna, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),GRP/FRP Profile Structures/Fiberglass Pultrusion Product.Suspended False Ceiling Decorated by Aluminum Honeycomb Composite Panel, 175-184[Garage Car Cover]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Rht-060ws Soda Process Water Cooled Screw Type Water Chiller]

Whitaker,J.R.(1963),High Efficiency Mining Sand Separator Hydrocyclone for Desliming & Dewatering. Analytical Chemistry35(12), 1950-1953[New Electric Piston Pump Coating Machine PT6900]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Villa Hotel Luxury Guest Room Decoration Standing Lamp (KA5506-5-195-160 G)Author for correspondence