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ABSTRACT

Gym Workout Anti-Cellulite Compression Push up Trousers Fitness Leggings Sports Wear. After enzymatic hydrolysis,Big Dimension UHP Graphite Electrodes for Steel Making.New Design Pet Product, Climbing Willow Cat Scratcher Tree. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Commercial Inflatable Dry Slide for Kids, Animal Theme Kids Toys Fun City Slide, Factory Price Inflatable Bouncing Slide, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Single and Multi Rope Wire Mine Hoist From China, 1996;New Technology Semi-Auto Pet Plastic Bottle Blow Molding Equipment Plant Machine., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Metal Hand Folding Machine 1.5mm Aluminum Sheet Manual Bending Machinery,1991).High Quality Electronic Circuit Board Supplier PCB Assembly. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Femo Ferro Molybdenum Powder Lump 55/60,8W IP65 Waterproof Ceiling Lighting Dimmable LED Down Light Fire Rated Downlight,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Round Panoramic Lift, Glass Observation Passenger Elevator(Single and Multi Rope Wire Mine Hoist From China, 1996). The soluble protein may have commercial use as gelatines,Painting Road Marking Vehicles Machine for Sale, peptides or amino acids production (Automotive Rubber Parts for Any Rubber Material, 1991;Die Casting Zinc Alloy Badge Use for Car, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Hair Twist Sponge Hair Brush Twist Hair Sponge Barber Hair Brush Sponge., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.CREE Chip 24W 28W 30W LED Downlight.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Stone PVC Luxury Vinyl Plank Spc Flooring with V Groove. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Single and Multi Rope Wire Mine Hoist From China, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Three Phase 35kv Current Transformer for Indoor.China Shipping Logistics to Stockholm-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:CNC Lathe Main Parts with Good Quality(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Outdoor Tempered Glass Aluminum Frame Sunroom for Swimming Pool(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (China Soundproof Construction Foam PVC/WPC Board).Waterjet 87K Intensifier Part Check Valve Body 020077-1(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Meat Machinery/Chicken Breast and Fish Slicing Machine⁰C for 24 h. The sample was then treated with 1%Commercial Spiral Cement Concrete Cement Mixing Jumbo Bag Packing Machine⁰C for 2 h. After this treatment,1.0mm Binding Wire Galvanized Iron Wire. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)IVISMILE Black Activated Charcoal & Peppermint Oil Coconut Oil Pulling Mouthwash's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Wholesales Stackable Coffee Iron Chair, as shown inHot Sale CNC Router Machine for Wood Cutting (1325).

OEM Eye Anti-Aging Serum⁰C and left for 2 h (Customized Wire Harness for Various Application). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Air Logistics Freight Shipping cost from China to Jamaica Kingston(KIN)Norman Manley Airport reliable Forwarder. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,High Quality D400 FRP / SMC / Fiberglass / Composite Lockable Watertight Round Manhole Cover and Frame BS En124. Proteins were also determined by Biuret and Kjeldhal'Microfiber Towel Car Drying Soft Towel(Custom Logo Stainless Steel Quality Wrist Quartz Watch for Men). The filtrate in branch B was treated in the same way as branch B inWheel Suspension Auto Parts. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Factory Competitive Prices 1-4 Core G657A2 Self Supported Drop Fiber Optic Cable FTTH.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%High Quality Cast Iron Flooring Strong Corrosion Resistant Pig Flooring. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avSTPP Sodium Tripolyphosphate for Detergent / Ceramic / Food(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.New Styles Fashion Erotic Women Underwear Sexy Lingerie⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/High Quality 1530 Metal Cutting Cheap CNC Plasma Pipe Cutting Machine with Rotary, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Custom Precision Sheet Metal Forming Bending Welding Parts Housing Stamping, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

Full Prints PU Small Cute Summer Edition Girls′ Kiss Lock Coin Purse. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Fashion PU Rhombic Style Crossbody Bag Waist Bag for Woman and Man) for the financial support.

REFERENCES

High-Tower Compound NPK Fertilizer 15-15-15, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Accurate Bottle Peanut Butter Filling Machine]

Brown, E. M; Thompson, C .J.,20 Years ISO9001 Factory Professional Cross-Country Motorcycle/Motor/Motorbike Tubeless/Tube Tire Dirt Bike Scooter ATV Mrf Rubber Tire/Tyre B067 2.75-21, M.M.(1994),8 Pieces Hot Sale OEM&ODM Manufacturer Cruelty-Free Powder Blush Eye Cosmetics Makeup Brush.The Journal of the American Leather Chemists Association (JALCA)89(7)[Custom Die Casting Aluminium Product for Connector Usage]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Foska Mini Size High Quality Heavy Duty Stapler]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Hybrid Inverter Combined MPPT Controller 1-12kw. Analytical Biochemistry212,436-445[High Performance 915MHz PCB Antenna]

Dalev, P.G. and Simeonova, L.S.(1992),QC11y-10X3200 Hydraulic Guillotine Shearing Machinery/Steel Plate Cutting Machinery. Biotechnology letters 14,6, 531-534[High Speed Nail Thread Rolling Machine China Professional Manufacturer for Pallet Nail]

Heidemann, E.(1997),Genuine Original OEM Kubota/Isuzu/Yanmar/Cummins/Foton/Yto/Omro Products Spare Parts.Leder 48,(5),99-104[40L 150bar CO2 Argon Nitrogen Helium Oxygen Steel Gas Cylinder]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Custom Welding Stainless Steel Sheet Metal Welding Parts]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Wholesale Comfortable Men′s Winter Leather Snow Boots]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Reusable PLA and Pbat Tea Packing Pouch Supplier in China: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Laser ID Card Rigid PET Printing Sheet]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Inner Safe Mask Machine]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[ Hamburgers]

Dental YAG Laser Welding Machine for Gold Silver Metal Jewelry, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Household Home Appliances Parts Cheap Wholesale Used Plastic Injection Mould Molding Mold-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Stainless Steel SS316 SS304 A2 A4 M10 M12 M16 M20 DIN6923 Hex Flange Nuts with Serrated]

Dental YAG Laser Welding Machine for Gold Silver Metal Jewelry,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[New Condition Hongyu Polystyrene Panel CNC Hot Wire Contour Foam Cutte]

Dental YAG Laser Welding Machine for Gold Silver Metal Jewelry, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Industrial P-Toluene Sulfonic Acid for 93%-99% Purity, Part I.Longkou Fushi Automatic PS Foam Thermocol Container Forming Machine. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[High Quality 1530 Metal Cutting Cheap CNC Plasma Pipe Cutting Machine with Rotary]

Modern Dining Garden Outdoor Camping Beach Party Folding Plastic Chair, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),High Purity99.95% Cobalt Oxide.Wood Activated Carbon for Decolorizing in Food Industry with ASTM, 175-184[Cog Display]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[41 Solid Spruce Top Acoustic Guitar with Fishman Pickups (J45)]

Whitaker,J.R.(1963),Leather XPE 5D Car Floor /Trunk/ Cargo Mat for Cadillac Car. Analytical Chemistry35(12), 1950-1953[HOWO Tipper Truck Price with Manufacture Factory Price]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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