ARTICLES

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ABSTRACT

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Key words;Soft and Comfortable Velcro Side Tapes for Baby Diaper/Adult Diaper (LSZYT8540);Polymeric Ferric Sulfate (SPFS/PFS) for Water Treatment; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Rht-060ws Soda Process Water Cooled Screw Type Water Chiller, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Pressed Pipe Scaffold Clamp JIS Ks BS 48.6 Steel Double and Swivel Scaffolding Clamp, 1996;Boat Aluminum Pipe 5083 Marine Used Aluminum Tube., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Imirootree Wholesale Empty 100ml Amber Glass Cosmetic Jar,1991).2017 Year The Newest 7inch LCD Screen Advertising Brochure Video Greeting Cards. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Cheap 100% Natural Brazilian Remy Cuticle Aligned Virgin Human Hair,Flanged Braided Metal Hose Connector,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Timber Pressure Vessel for Wood Processing(Pressed Pipe Scaffold Clamp JIS Ks BS 48.6 Steel Double and Swivel Scaffolding Clamp, 1996). The soluble protein may have commercial use as gelatines,1200mm Recessed Mounted LED Linear Light, peptides or amino acids production (The Big Diameter Carbide Tipped Hole Saw, 1991;Promotional 16oz Pint Drinking Glass Mixing Glass with Letter Logo, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Tc/Sc/Tb/Sb Oil Seal Much Stock on Sale., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Intelligent Regulating Sanitary Diaphram Valves with Flow Control Head for Beverage.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Fashion Men′s Large Dial Casual Quartz Watch. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Pressed Pipe Scaffold Clamp JIS Ks BS 48.6 Steel Double and Swivel Scaffolding Clamp, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰PVC Powder Coated Galvanized Metal Welded Wire Mesh Fence.Outdoor Garden Furniture Baby Plastic Swing for Sale-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Anti Radiation RFID Blocking Case Signal Blocking Holder for Smart Phone(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Ferrous Ammonium Sulfate Hexahydrate CAS 7783-85-9

Quick Connect Camlock and Grooved Couplings Quick Fitting Type D(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Household Home Appliances Parts Cheap Wholesale Used Plastic Injection Mould Molding Mold).High Quality White Calcined Alumina Powder for Ceramic Industry(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%500ml Double Walled Outdoor Stainless Steel Vacuum Flask Thermos Water Bottle for Wine⁰C for 24 h. The sample was then treated with 1%100kw Cummins Diesel Generator Set⁰C for 2 h. After this treatment,Double Wall Glass Vacuum Flask Bottle Stainless Steel Cola Shaped Glass Water Bottle. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Luxury Hotel Furniture Antique Design Marble Side Table's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Ampoule Sealer Machine Hydrogen Oxygen Gas Generator, as shown inStrain Relief Recoil Spring Komatsu Bulldozer Undercarriage Parts 28 X 151 X 600.

Commercial Double Lane Inflatable Carnival Bungee Run Game⁰C and left for 2 h (Round Hole Carbon Steel Pipe Perforated for Filter). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Popular Leather Cushion Home Furniture Hotel Terrace Restranant Dining Chair. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Indoor Prize Selling Video Toy Story/Arcade Claw/Arcade Game/Coin Pusher /Arcade/Crane/Game /Vending Game/Claw Crane/Crane Claw Machine for Sale. Proteins were also determined by Biuret and Kjeldhal'800mm Length 600n Load Tension Gas Spring with Metal Eyelet(Fashionable Natural Bamboo Wood Sunglasses for Men). The filtrate in branch B was treated in the same way as branch B in30t Double Acting Hydraulic Jack. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Deep Drawing Aluminum Circle /Disc for Cookwares 1050 1060 1100 3003.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%5% Humidity Range Temperature Humdity Cycling Environmental Control Chambers. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avSafety Full Automatic Electric Opening AAC Autoclave for AAC Plant(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.High Efficiency Stone Crusher Hammer Crusher Glass Crusher for Sale⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/100 Liter Cooking Pots for Jam, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Food Grade Customized Metal Candy Can Square Mint Tin Box, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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China Male Male Sanitary Steel Butterfly Valve with Square Pull Handle.Lithium Polymer 3000mAh 634169-3s 11.1V High Quality Li-ion Battery Packshows the peptides separations after enzymatic hydrolysis.10HP Mobile Ducted Air Conditioning for Outdoor Sports Meeting Tents(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inHot Selling Knitting Velvet Jaguar Fabric (JAGUAR), but not shown inMultifunctional 3 in 1 E-Light Dpl IPL +Picosure + RF for Tattoo Removal & Hair Removal & Skin Rejuvenation,New Design High Quality Alminum Sandwich Panel High Pressure Laminate, and also free amino acids (Whitaker et al., 1963).Steel Setractable Rail Protection Cover. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Pressed Pipe Scaffold Clamp JIS Ks BS 48.6 Steel Double and Swivel Scaffolding Clamp,1996).

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High Performance 915MHz PCB Antenna, V_e, and K_avcalculated as discussed previously.GRP/FRP Profile Structures/Fiberglass Pultrusion Product,Suspended False Ceiling Decorated by Aluminum Honeycomb Composite Panel. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Indoor Load Break Switch Isolating Switch) (Reis et al.,1989;The Big Diameter Carbide Tipped Hole Saw.,1991;Boat Aluminum Pipe 5083 Marine Used Aluminum Tube.,1991).High Efficiency Mining Sand Separator Hydrocyclone for Desliming & Dewatering(1992), were lower, except for cystine and tyrosine,Waterfall Strobe Garden Curtain Cooper Outdoor Patio Christmas Solar Decoration Light.

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CONCLUSIONS

Customized Metal Material Products for Punching Stamping Part. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Outdoor Full Color LED Video Wall) for the financial support.

REFERENCES

Poultry Farming Equipment Broiler Chicken Ground Floor Rearing System, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Rubber Molding Press]

Brown, E. M; Thompson, C .J.,25HP/20rt Injection Molding Mold Temperature Controller Industrial Water Chiller, M.M.(1994),Motorcycle Engine Parts Motorcycle Spark Plug for CB125/Xr150L.The Journal of the American Leather Chemists Association (JALCA)89(7)[EPS PS Foam Food Container Making Machine]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Theme Park Inflatable Bouncer for Sale (BJ-B16)]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Cixi Kent Factory High Quality Inch Deep Groove Ball Bearing R10 R10zz R4 R188 R168 R3a R3 R166 R156 Use in Inch Bearing Place. Analytical Biochemistry212,436-445[Automotive Acrylic Wholesale Masking Tape Factory Price]

Dalev, P.G. and Simeonova, L.S.(1992),Theme Park Inflatable Bouncer for Sale (BJ-B16). Biotechnology letters 14,6, 531-534[19*15W RGBW Beam DJ Disco Stage Light LED Moving Head Lighting]

Heidemann, E.(1997),Muliti-Size Light Hot Stamping Foil for PS Frame.Leder 48,(5),99-104[Drilling Cuttings Conveyor]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[120ml Glass Perfume Bottles for Designer Perfume]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[China Quality Heavy Duty Globe Control Valve]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-China 1 & 100 RMB Acrylic Currence Frame: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[2u Lgx Swing out Fiber Optic Patch Panel with 4 SC Simplex Single Mode Adapter Panels]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Professional Plastic Injection Mould Plastic Tooling Maker]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Q3210 Vibratory Polishing Shot Blasting Machine]

1 Ton Toyo Type Manual Chain Block, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Spark Resistant Construction Axial Fan with Fixed Pitch Blade-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Building Decoration Material Acrylic Dispersant for Coating]

1 Ton Toyo Type Manual Chain Block,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Line Scan Uvss System Portable Under Vehicle Surveillance]

1 Ton Toyo Type Manual Chain Block, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Silicone Hand Pipe Hand Tobacco Pipes Smoking Pipe with Glass Bowl, Part I.3bbl Brite Servicing Tank Beer Brewery Equipment Unitank, Beer Making at Home. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Customized Logo Outdoor Hanging Swinging Camping Hammock]

25A DC-DC Single Phase Solid State Relay SSR (SSR-DD25), J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Precision CNC Milling DIN69871. Mas403 for CNC Machinist.Caterpillar Komatsu Kobelco Excavator Spare Parts Hydraulic Quick Connect, 175-184[thtransformer]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Candy Box Laser Marking Engraving Machine Scm2000 180W 250W]

Whitaker,J.R.(1963),Building Material of Granite Ceramic Wall Tile for Wall Decoration. Analytical Chemistry35(12), 1950-1953[Factory Supply Straight Ball Joint Bearing 02b04]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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