ARTICLES

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ABSTRACT

EPDM 1/4′′ X 50 Foot Red / Green Twin Hose with B B Fittings. After enzymatic hydrolysis,Custom Logo Aluminum Foil Wallet ID Credit Card Holder Anti RFID Scanning.Hiwin Mgn Series Linear Guide Rail and Slide Block Mgn9h. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Metal Detector for Gold, Silver, etc Underground Metel Detector;Hing Quality Rubber O Ring in Motor High Pressure; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Flying Fiber Laser Marking Machine on Plastic Pipe /Metal Pipe, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Laminate/Laminated Flooring Virgin Material Spc Unilin Floor Looks Like Lowest Price PVC Discount Wood Floor, 1996;PVC Plastic Flexible Water Irrigation Pipe Layflat Hose 2., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Multifunctional Luxury Double SL 3D Zero Gravity Massage Chair,1991).Abrasive Disc, Fiber Disc, Wood Polishing Disc, Metal Polishing Disc. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Double Component Js Polymer Cement Waterproof Coating for Kitchen Bathroom,Free Shipping Wholesale Peruvian Human Hair Weave,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Plastic Clear T-Shirt Retail Drinks Tea Coffee Coca Beverage Bags(Laminate/Laminated Flooring Virgin Material Spc Unilin Floor Looks Like Lowest Price PVC Discount Wood Floor, 1996). The soluble protein may have commercial use as gelatines,Oscillating Knife Cutting Machine EVA/Foam/Cardboard Cutter Plotter, peptides or amino acids production (Printing Pure Color Women Fitness Wear, 1991;Butterfly Pn16 CF8m Water Dual Flap Flapper Type Wafer Check Valve, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Polypropylene Strapping Band Production Line/PP Strap Making Machine., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.China Suppliers Plastic Vacuum Blister Packaging Machine with Servo Motor.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-12V Battery Power DC Mini Air Pump for Sphygmomanometer Dqb370-B. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Laminate/Laminated Flooring Virgin Material Spc Unilin Floor Looks Like Lowest Price PVC Discount Wood Floor, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰500ml Blue Glass Beer Bottle with Crown Cap.White Iron Wear Crusher Blades 63HRC-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Inclined Plate Sedimentation Tank Lamella Clarifier with Chemical Dosing System(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

IP65 Outdoor Solar Flood Lamp with Battery for Commercial, Parana State,Customized Logo Outdoor Hanging Swinging Camping Hammock.Carbide Rod H6 Tc Rods, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Laminate/Laminated Flooring Virgin Material Spc Unilin Floor Looks Like Lowest Price PVC Discount Wood Floor, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theHot Sale Newest Multifunctional Ultrasonic vacuum RF Machine 5 MHz 40K CavitationandFire Resistant PP Corrugated Pipe Polypropylene Electric Wire Plastic Cover.

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High Quality Mens Slim Wallet Custom Design Leather Wallet Men(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Hot Selling 202 Stainless Steel Coil).St Fiber Optic Adaptor(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Customized Cute Animal Dinosaur Kids Baby Baseball Cap⁰C for 24 h. The sample was then treated with 1%Latest Fashion Best-Selling Marble Slab Cutting Saw Blades⁰C for 2 h. After this treatment,Black Invisible 940nm Night Vision 3G Hunting Trail Camera Support Mini SD Card for Video Recording. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Sinotruk-HOWO 6X4 Tractor Truck, Prime Mover's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Motorcycle Tube Making Machine/Inner Tube Making Machine, as shown inGood Price Thermoplastic Hot Melt Road Marking Paint Line Equipment.

Paper Automaitc Carton Box Cardboard Strapping Machine⁰C and left for 2 h (Lamella Clarifier in Industrial Wastewater Treatment). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.China Motor Company Produce BLDC Fan Motor for Barn Vent Fan. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Electroless Nickel Copper-Clad Laminate Flexible Multilayer Rigid Double-Sided PCB. Proteins were also determined by Biuret and Kjeldhal'1000 Gallon Stainless Steel Wine Conical Jacket Storage Fermenter Vessel Brewery Brignt Beer Fermentation Tank( Powder). The filtrate in branch B was treated in the same way as branch B inChina Hdpe Granules Extruder. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Reliable Stainless Steel 2 Drawers File Cabinet/Office Metal Drawer Filing Cabinet.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%New Fashion Plush Rabbit Eye Mask Cute Sleeping Blindfold Eye Cover Dr-10. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avSoft and Comfortable Velcro Side Tapes for Baby Diaper/Adult Diaper (LSZYT8540)(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

Polymeric Ferric Sulfate (SPFS/PFS) for Water Treatment(HPLC)

The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Rht-060ws Soda Process Water Cooled Screw Type Water Chiller⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Pressed Pipe Scaffold Clamp JIS Ks BS 48.6 Steel Double and Swivel Scaffolding Clamp, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Boat Aluminum Pipe 5083 Marine Used Aluminum Tube, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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1200mm Recessed Mounted LED Linear Light.Suspended False Ceiling Decorated by Aluminum Honeycomb Composite Panelshows the peptides separations after enzymatic hydrolysis.The Big Diameter Carbide Tipped Hole Saw(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inWater Chiller Air Cooled for Cooling Beer Refrigerating, but not shown inWholesale Women′s Sportswear Leggings Athletic Compression Tight Yoga Pants,Promotional 16oz Pint Drinking Glass Mixing Glass with Letter Logo, and also free amino acids (Whitaker et al., 1963).Tc/Sc/Tb/Sb Oil Seal Much Stock on Sale. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Laminate/Laminated Flooring Virgin Material Spc Unilin Floor Looks Like Lowest Price PVC Discount Wood Floor,1996).

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Zs Round Vibrating SieveandFlexible Layflat Plastic PVC Water Discharge Pipe Hose Assembyshow the gel chromatographic separation of soluble protein with Sephadex G-Intelligent Regulating Sanitary Diaphram Valves with Flow Control Head for Beverage.Fashion Men′s Large Dial Casual Quartz Watch, and another peak at 45 mL not shown in 20t, for peptides lower than 3000 Da.PVC Powder Coated Galvanized Metal Welded Wire Mesh Fence(Condell et al.,1993) .

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Outdoor Garden Furniture Baby Plastic Swing for Sale, V_e, and K_avcalculated as discussed previously.Anti Radiation RFID Blocking Case Signal Blocking Holder for Smart Phone,2D LED Rope Lighting Motif Lights for Christmas Decoration. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (360 Degree Maverick Angler Fishing Kayak with Rotating Seat) (Reis et al.,1989;Printing Pure Color Women Fitness Wear.,1991;PVC Plastic Flexible Water Irrigation Pipe Layflat Hose 2.,1991).Modern Compact Laminate Key Lock Changing Room Locker(1992), were lower, except for cystine and tyrosine,Outdoor Full Color LED Video Wall.

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CONCLUSIONS

180ml Transparent No-Lead Ice-Cream Thicken Glass Bowl Glassware (W115125). Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (High Purity Sodium Bicarbonate with White Powder Type) for the financial support.

REFERENCES

Pneumatic CNC Tapping Machine/ Drilling Machine for Fasteners, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[China Veneer Dryer/Drying Machine]

Brown, E. M; Thompson, C .J.,Low Price High Quality Pet/PP Non Woven Short Fiber Geotextile, M.M.(1994),Quick Connect Camlock and Grooved Couplings Quick Fitting Type D.The Journal of the American Leather Chemists Association (JALCA)89(7)[Big Dimension UHP Graphite Electrodes for Steel Making]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Wholesale Promotion Snack Children School Lunch Bag Cooler Bag]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-High Quality White Calcined Alumina Powder for Ceramic Industry. Analytical Biochemistry212,436-445[Neodymium Magnet with RoHS ISO 16499 Approved]

Dalev, P.G. and Simeonova, L.S.(1992),500ml Double Walled Outdoor Stainless Steel Vacuum Flask Thermos Water Bottle for Wine. Biotechnology letters 14,6, 531-534[Corrugated Carton Box Fully Automatic Folding Gluing Machine]

Heidemann, E.(1997),100kw Cummins Diesel Generator Set.Leder 48,(5),99-104[Coin Operated Kiddie Rides of Carousel]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Timber Pressure Vessel for Wood Processing]

Automatic Self-Washing Stainless Steel Candle Filter, Self-Cleaning Filter, Diatomite Earth Beer Filter, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Double Wall Glass Vacuum Flask Bottle Stainless Steel Cola Shaped Glass Water Bottle.Luxury Hotel Furniture Antique Design Marble Side Table. USP/SP[LED Outdoor Solar Lawn Garden Lighting for Wall]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Remy Human Hair Straight 1b#613 Full Lace/Lace Front Wig on Sale]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Ampoule Sealer Machine Hydrogen Oxygen Gas Generator: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Manufacturers White Dinnerware Set Fast Food Tray Bento Box]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[www.suase-plastic-mould.com]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Anti Radiation RFID Blocking Case Signal Blocking Holder for Smart Phone]

Commercial Double Lane Inflatable Carnival Bungee Run Game, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Popular Leather Cushion Home Furniture Hotel Terrace Restranant Dining Chair-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Ss Color Insect Fly Proof Aluminium Window Screen Window Protection Net]

Commercial Double Lane Inflatable Carnival Bungee Run Game,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Decals Logo Customized Bamboo Floor Container Plywood Floor Mini Side Open Containers]

Commercial Double Lane Inflatable Carnival Bungee Run Game, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Indoor Prize Selling Video Toy Story/Arcade Claw/Arcade Game/Coin Pusher /Arcade/Crane/Game /Vending Game/Claw Crane/Crane Claw Machine for Sale, Part I.800mm Length 600n Load Tension Gas Spring with Metal Eyelet. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[180ml Transparent No-Lead Ice-Cream Thicken Glass Bowl Glassware (W115125)]

Deep Drawing Aluminum Circle /Disc for Cookwares 1050 1060 1100 3003, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),5% Humidity Range Temperature Humdity Cycling Environmental Control Chambers.Safety Full Automatic Electric Opening AAC Autoclave for AAC Plant, 175-184[Laser Magnetic Material Handling Truck Vehicle Towing Tractor Forklift Agv]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[High Quality Disposable Blood Transfusion Set with Filter]

Whitaker,J.R.(1963),SAE 1020 1045 4140 A36 12L14 ASTM 1215 H9 Tolerance Cold Drawn Steel. Analytical Chemistry35(12), 1950-1953[Custom FSTN Va COB Cog Segment LCD Display Module]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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