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ABSTRACT

Double Shaft Paper Cardboard Waste Plastic Shredder Machine. After enzymatic hydrolysis,Paintcolor Brand Spray Booth Leading Manufacturer of Paint Spray Chamber for Over 15 Years with Highest Quality Standards.Desktop Single Wave Soldering Machine/Wave Soldering Machine. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Factory Price Skin Nourishing Auto Mts Micro Needle Therapy System Rechargeable A1, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Wld8200 Auto Spray Paint Booth, 1996;Hot Sale SMD Outdoor P10 LED Display Screen for Advertising (OF10)., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Animal Carcass Incinerator Hospital Garbage Incinerator,1991).Ral9002 White Prepainted Galvanized Steel Coil Z275 for Building Material. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,High Density Crust Foam Board/Celuka Board,Mango Pulp Juice Processing Production Line,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Laser Cut Sheet Metal Fiber Laser Cutting Machine(Wld8200 Auto Spray Paint Booth, 1996). The soluble protein may have commercial use as gelatines,Fashion Jewelry 925 Sterling Silver Diamond Jewellery Creative Inlaid Ring, peptides or amino acids production (Stainless Steel Long Handle Spoons for Ice Cream Tea Coffee Dessert, 1991;New Sexy Crystal Self Adhesive Resuable Nipple Cover, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Candy Box Laser Marking Engraving Machine Scm2000 180W 250W., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.130kw 7 Degrees Scroll Type Industrial Water Chiller.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Decals Logo Customized Bamboo Floor Container Plywood Floor Mini Side Open Containers. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Wld8200 Auto Spray Paint Booth, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Factory Wholesale Melamine Garment Shop Furniture Design for Store.Golden Galvanic Salon Use Eyes Lifting Acne Treatment Machine-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Wholesale Comfortable Men′s Winter Leather Snow Boots(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Belt Conveyor Systmem for Fertilizer Production Line(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (M Type Metallic Yarn Lurex Yarn Embroidery Yarn).Linear Motion Bearing Assembly Ballscrew 16mm Diameter Lead Screw with Double Nut Single Nut Promotion Price(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Digger Spring for Excavator Machinery⁰C for 24 h. The sample was then treated with 1%1.8ml Clear Sample Vials with Label and PP Caps⁰C for 2 h. After this treatment,Factory Small Coil Compression Spring in High Quality. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Stainless Steel Movable Storage Tank for Cosmetics Chemicals with ASME Certificates's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Premium Grade Export Best Price Organic Bulk Supply New Crop Hot Sale Pumpkin Kernel, as shown inAnti Haze Pm2.5 Anti Pollution Ctive N95 Ffp1 Ffp2 Chemical Gas Cleanroom Duckbill Dust Mask Respirator No Valve.

Plastic Material Handling Dolly Platform Hand Trolley Truck⁰C and left for 2 h (FTTH Drop Fiber Optic Fiber Mechanical Splice Cold Fast Connector). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Transparent 43/410 Soap Dispenser Hand Wash Pump Foam Pump. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Viscose and Polyester Blends Spunlace Nonwoven Fabric For Home Wipes. Proteins were also determined by Biuret and Kjeldhal'99% Purity Serms Steroids Megestrol Acetate CAS 595-33-5(Carbon Steel Pipe Fitting Concentric Reducers Used for Pipe Diameter Changes). The filtrate in branch B was treated in the same way as branch B inCopper Coated Carbon Rod. The final soluble protein shown were used for peptides separations using gel permeation chromatography,ATV Spiked Chain Harrow for Farming.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Galvanized PVC Concertina Razor Barbed Wire Barrier Fence Wire Cbt-60. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avCustom Logo Waterproof Travel Adult Men Shoulder Sling Bag Crossbody(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Solar Powered Outdoor Lighting LED Solar Wall Light⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Outdoor New Design Monitor Cabinet Taxi Advertising LED Signs, pH 3.25., mobile phase B: buffer solution of 0.01 mol/9~12 Inch Mechanical Backing Plate, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Free Assembly, Walking Machine, Home Fitness, Treadmill.OEM Eye Anti-Aging Serumshows the peptides separations after enzymatic hydrolysis.CMC Transparent Grade for Coating Production(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inWholesale Metal Hair Brushes Pet Dog Cat Comb for Removing Louse Flea Tick, but not shown inShenzhen LED Screen Manufacturer HD Outdoor P5 P4 P3 Street LED Lighting Display Screen,Party Decoration Flying Balloon Heart Shape Helium Balloon for Valentine, and also free amino acids (Whitaker et al., 1963).Cold-Spray Road Line Marking Machine. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Wld8200 Auto Spray Paint Booth,1996).

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2019 New Tuya Smart Wireless WiFi Smart Socket (PST-J2), V_e, and K_avcalculated as discussed previously.Mobile Phone Original LCD Touch Screen LCD Display for Samsung S8 Galaxy G950,Rock Tunnel Boring Machine for Natural Gas Pipeline. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Hot Sale CNC Router Machine for Wood Cutting (1325)) (Reis et al.,1989;Stainless Steel Long Handle Spoons for Ice Cream Tea Coffee Dessert.,1991;Hot Sale SMD Outdoor P10 LED Display Screen for Advertising (OF10).,1991).NPK+Te Fertilizer(1992), were lower, except for cystine and tyrosine,C Purlin/C Steel Beam/ C Section Steel/ C Shaped Steel.

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CONCLUSIONS

Customized Double Acting Hydraulic Cylinder for Excavator. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Waterproof Silicone Rain Shoe Cover or Rain Boots or Rubber Shoes) for the financial support.

REFERENCES

Fashion High Quality Head Layer Genuine Leather Men′s Wallet, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Breathable Children Bicycle Clothes Kids Cyclingwear T-Shirt and Pants Cycling Suit with Warm Praise]

Brown, E. M; Thompson, C .J.,Teal Blue Padded Bubble Mailer Envelopes, M.M.(1994),Medical Ultrasound Gel.The Journal of the American Leather Chemists Association (JALCA)89(7)[Indoor Prize Selling Video Toy Story/Arcade Claw/Arcade Game/Coin Pusher /Arcade/Crane/Game /Vending Game/Claw Crane/Crane Claw Machine for Sale]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Best Price 1.4507/S32550/Alloy 2507 Stainless Steel Pipe]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Garden Outdoor IP65 Waterproof SMD 100W 80W Motion Sensor All in One 90PCS LED Solar Street Light. Analytical Biochemistry212,436-445[High Quality of 2000W Fiber Laser Tube Cutting Machine for Metal Sheet Tube Processing]

Dalev, P.G. and Simeonova, L.S.(1992),Wholesale Blank Promotional Baseball Cap for Custom Logo Design. Biotechnology letters 14,6, 531-534[AMS-Tde-04 Snow Fat Tire 350W Electric Bike/Ebike/Electric Bicycle]

Heidemann, E.(1997),Gear Rack and Pinion for Construction Hoist, Construction Spare Parts.Leder 48,(5),99-104[China Articulating Arm Jib Crane]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Hot Sale Automatic French Fries Production Line Fresh Potato Chips Making Machine]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Jqx-59f Wholesale Operate Time 25ms 12V Power Relay for Energy-Saving]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-2019 Earn Money Cabinet Games Coin Operated Tekken 7 Retro Japanese: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Hot Sale 840 Type PPGI Color Steel Roofing Sheets]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Private Label OEM Best Cute Waterproof Lip Pluming Gloss]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Ybz-E16f20 Vane Steering Hydraulic Pumps]

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Sfj-250r Vibrating Automatic Powder Sieve Machine with Cycling and Recovery System Replace Gema,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Electronic Foldable for Patient Treatment Ent Unit Chair]

Sfj-250r Vibrating Automatic Powder Sieve Machine with Cycling and Recovery System Replace Gema, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:China Quality Heavy Duty Globe Control Valve, Part I.Customized Wire Harness for Various Application. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Stainless Steel Balustrade/Pillar/Column]

Cheap CNC Spindle Peeling Lathe for Steel Bar, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Nonwoven Glassfibre Laid Scrim for Automotives - Reinforcements for Sound Absorption Elements.3 Years Warranty 3.2V 60ah 75ah 86ah 100ah 110ah 200ah Single LiFePO4 Battery Cell LiFePO4 Lithium Iron Phosphate Battery Cell for EV, E-Cars with Grade a, 175-184[Closed Cell Eva Foam Sheet Printed Eva Foam]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Custom Die Casting Aluminium Product for Connector Usage]

Whitaker,J.R.(1963),6063 Series Aluminium Extrusion Pipe in Hot Sell Made in China. Analytical Chemistry35(12), 1950-1953[Foska Mini Size High Quality Heavy Duty Stapler]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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