ARTICLES

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ABSTRACT

Steel Floor Deck Post Cutting Cold Roll Forming Machine. After enzymatic hydrolysis,Similar Kavo8000b Fiber Optic Turbine with Water Volume Adjustable Coupling (KV180).Fluorous Rubber Coated Fiberglass Cloth. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.PS Foam Disposable Fast Food Box Making Machine, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (K Helical Bevel Gearboxes Reducer Motor, 1996;Indoor Customized Electronic Lighted LED Neon Open Sign., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Ecofriendly &Economic Jar Manufacture Cute Glass Handle Candy Jar,1991).Fashion Buttons Clothing Accessories Eco-Friendly Nickel-Free Metal Blue Button. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Custom Free Logo Design Cheap Hand Flag (NF01F02021),125kHz RFID Card 13.56MHz Proximity Card Blank Thin Card,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Ultra Slim Crystal Seashell Design Shockproof Phone Cases for iPhone X(K Helical Bevel Gearboxes Reducer Motor, 1996). The soluble protein may have commercial use as gelatines,RJ45 Cat5e in-Line Coupler Modular Jack, peptides or amino acids production (Amazon Inserts Electric Fireplace with Gas Heaters Fan Modern Furniture, 1991;Three-Dimensional 3D Fashion Design Wall Covering Material PVC Wallpaper, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Cold Rolled Coated Aluminum Coils 3003 H24 /1060/3004/5052., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.High Quality Disposable Blood Transfusion Set with Filter.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-DIN1478 Closed Body Jaw Jaw Wire Rope Turnbuckle. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (K Helical Bevel Gearboxes Reducer Motor, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Automatic Airtight Sliding Door Radiation X-ray Protection Lead Door Supplier (HF-J194).High Quality Brushless DC Electric Motor for Industrial Deduster-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:China Factory Light Soda Ash /Sodium Carbonate(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

350mm Diamond Blade for Granite, Parana State,HVAC Contactor /Air Conditioner Contactor /Definite Purpose Contactor.Custom Welding Stainless Steel Sheet Metal Welding Parts, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (K Helical Bevel Gearboxes Reducer Motor, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to thePlastic Packaging Tray Vacuum Forming MachineandSamsung LTI550HN16 1920X1080P monitor screen LCD video wall.

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Unbreakable 60W 140lm/W 2*1500mm LED Waterproof IP65 Linear Light Lamp(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Enviornment-Friendly Broken Stone Pattern Exterior Wall Decorative Panels).Laser ID Card Rigid PET Printing Sheet(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%New Arrival Plush Animal Ride for Kids for Shopping Center⁰C for 24 h. The sample was then treated with 1%Professional IPL Shr E-Light Opt Diode Laser Hair Removal Beauty Machine⁰C for 2 h. After this treatment,(JC-3080) High Quality Clothing Wash Care Ultrasonic Label Cutting Machine. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Ss316 Stainless Steel Heavy Duty Straps's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)6X420mm Headband Binocular Loupes Dental Magnification Surgical Lightweight Hesperus, as shown inHigh Precision IP54 Waterproof Digital Vernier Caliper 6 Electronic Caliper (WW-54DC04).

High Temperature Side Opening AAC Autoclave, AAC Block Making Machine⁰C and left for 2 h (China Manufacturer Cheap Custom Running Award Metal Sport Trophie Medal). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.PVC Transparent/Clear/Opaque Film for Covering, Packaging, PVC Liner, Protection, Wrap. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Multifunctional 3 in 1 E-Light Dpl IPL +Picosure + RF for Tattoo Removal & Hair Removal & Skin Rejuvenation. Proteins were also determined by Biuret and Kjeldhal'Teflon Seal/Seals Spring Energized, PTFE Spring Energized Seal(Metal Container Packaging for Edible Oil Tin Cans Storage Boxes). The filtrate in branch B was treated in the same way as branch B in200-400mm Pipe Belling Machine. The final soluble protein shown were used for peptides separations using gel permeation chromatography,1929 Weifang Huamei Home Use Handy Skin Care IPL Shr Laser Hair Removal Machine.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%New Mason Cup Frosted Drink Bottle Juice Glass Mason Jar. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avGreen Color PVC Anti Dazzle Panel(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.XL-21 Motive Electrical Switchgear Power Cabinet⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Digital Converter 110VAC 60Hz to 220VAC 50Hz Sine Wave Frequency Inverter, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Furniture Grade E1 Glue Plywood with Competitive Price, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

High Pulling Force Rotatable Rare Earth Crane Magnet Hook. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Solar Photovoltaic Connector Mc4 Connector for Solar System (PV-MC4)) for the financial support.

REFERENCES

1g, 2g, 5g, 20g Silica Gel Desiccant for Food Products, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Belly Button Piercing]

Brown, E. M; Thompson, C .J.,Blank Custom Fitness Leggings Women High Waisted Compression Yoga Leggings for Women, M.M.(1994),High-Performance Thermal Break Profiles Aluminium for Doors and Windows.The Journal of the American Leather Chemists Association (JALCA)89(7)[Wood Activated Carbon for Decolorizing in Food Industry with ASTM]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Fea040b New Arrival Airplane Seat Belt Extender]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Design Luxury Moon Cake Box with Hot Stamp Logo. Analytical Biochemistry212,436-445[ 5g]

Dalev, P.G. and Simeonova, L.S.(1992),Standard Industrial Double Nut Ball Screw Dfu Series. Biotechnology letters 14,6, 531-534[Cast Floating Ball Valve China Manufacturer (GAQ41F)]

Heidemann, E.(1997),Automatic Self-Washing Stainless Steel Candle Filter, Self-Cleaning Filter, Diatomite Earth Beer Filter.Leder 48,(5),99-104[Best Selling Football Goal Soccer Hand Flag for Sale]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Heat Resistant Adhesive Teflon Tape Price High Temperature Teflon Tape]

Kubota Combine Harvester Spare Parts 5h484-2392 Nut, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Custom LCD Video Brochure Card/Video Greeting/Wedding Card/Video Business Card.Customized Logo Outdoor Hanging Swinging Camping Hammock. USP/SP[Hot Sale Smoking Accessories Silicone Smoking Pipes Tobacco Hand Pipes with Glass Bowl]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Industrial Cast Steel Flanged One Way Swing Check Valve Nrv]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-EPDM 1/4′′ X 50 Foot Red / Green Twin Hose with B B Fittings: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Biobase China Vertical Type-Double Doors -25º C Freezer for Best Price]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Popular Dog Food Pellet Making Machine Production Line]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Digital Converter 110VAC 60Hz to 220VAC 50Hz Sine Wave Frequency Inverter]

Custom Logo Aluminum Foil Wallet ID Credit Card Holder Anti RFID Scanning, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Hiwin Mgn Series Linear Guide Rail and Slide Block Mgn9h-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[High Speed HDMI Cable Wholesale HDMI with Ethernet]

Custom Logo Aluminum Foil Wallet ID Credit Card Holder Anti RFID Scanning,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[ SLA Shoes Prototyping for Auto Accessory]

Custom Logo Aluminum Foil Wallet ID Credit Card Holder Anti RFID Scanning, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Metal Detector for Gold, Silver, etc Underground Metel Detector, Part I.Hing Quality Rubber O Ring in Motor High Pressure. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Pressed Pipe Scaffold Clamp JIS Ks BS 48.6 Steel Double and Swivel Scaffolding Clamp]

Flying Fiber Laser Marking Machine on Plastic Pipe /Metal Pipe, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Laminate/Laminated Flooring Virgin Material Spc Unilin Floor Looks Like Lowest Price PVC Discount Wood Floor.PVC Plastic Flexible Water Irrigation Pipe Layflat Hose 2, 175-184[Plant Fiber Decorative Mural 3D Wallpaper 3D Wood Wall Panels for Home Deco]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Sludge Oil Separation Centrifuge]

Whitaker,J.R.(1963),Multifunctional Luxury Double SL 3D Zero Gravity Massage Chair. Analytical Chemistry35(12), 1950-1953[Happy Cat Tree Condo Pet Scratcher Tower Small Black Sisal Scratch Post]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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