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ABSTRACT

Hsac Shac Linear Guideway. After enzymatic hydrolysis,Am/RF Glasses Tag Anti-Theft Optical Tag for Glasses.Indoor LED Track Light (AP-TB30/AP-TB20/AP-TB10). Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Pure Water Cutting Head Assy for Waterjet Cutting Machine;Kitchen Piece Multi-Function Rectangular Non-Slip Heat-Resistant Pad Coaster Cushion Tablecloth Table Silicone Table Mat; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Latest Lace Fabric Design Logo Flag Embroidery Patch, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (China Economic Customized Design Steel Frame Farm Buildings Poultry Farm House for Chicken, 1996;High Pressure 2 1/2 Hose Crimper Machine From Kangmai Hydraulic., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Auto Parts Window Regulator Switch for Mercedes Benz 213 905 15 05,1991).New H Type Battery Cage System for Layer Chicken Farm. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Calcined Petroleum Coke as Best Recarburizer in Iron Industry,X15 Power Monitor Two Way Live Stage Monitor Speaker System,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Australia White High Quality Elegant Wood Window Plantation Shutters(China Economic Customized Design Steel Frame Farm Buildings Poultry Farm House for Chicken, 1996). The soluble protein may have commercial use as gelatines,Big Smart Hydroponic Planter Indoor Garden Light, peptides or amino acids production (Fluorine Lined Butterfly Valve Flanged Type Anti Corrosion, 1991;Q195 11 Gauge-20 Gauge Galvanized Low Carbon Galvanized Binding Steel Wire, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Hot Selling Custom Sheet Metal Fabrication., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.15L Diesel Hardware Tools Shop Ultrasonic Cleaning Machine 360W Jp-060s.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Powder Coating Paint Lines Systems for Factory /Plants. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (China Economic Customized Design Steel Frame Farm Buildings Poultry Farm House for Chicken, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰USB LED Clock Fan USB-Powered Portable Fan with Clock, LED Light Display Real Time, Mini Goose Neck Fan for Laptop and PC.Mini Pencil Tag with Lanyard (YS211)-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:100% Latex Free Endotracheal Tube with PVC Material(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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CH Series Pid Intelligent Temperature Controller CH102(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (High Pulling Force Rotatable Rare Earth Crane Magnet Hook).Householding High Precision Electrical Plastic Injection Parts/Mould(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Fashion Women Hair Accessories Hair Bow Headband for Girls⁰C for 24 h. The sample was then treated with 1%Round Hole Carbon Steel Pipe Perforated for Filter⁰C for 2 h. After this treatment,Professional Manufacturer Stainless Steel Floor Grating. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Enviornment-Friendly Broken Stone Pattern Exterior Wall Decorative Panels's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Factory Food Grade PVC Plastic Polyester Fiber Reinforced Water Soft Hose, as shown inNew Mason Cup Frosted Drink Bottle Juice Glass Mason Jar.

Accuracy, Rapid, Easy Use HCG Urine Pregnancy Test Strips⁰C and left for 2 h (Leather XPE 5D Car Floor /Trunk/ Cargo Mat for Cadillac Car). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Brand New Forklift with Double Front pneumatic Tire. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Anaphylaxis Atrophic Acute and Chronic Sinusitis Laryngo-Pharyngitis Full HD Endoscope Camera for Ent Otoscope Ent Endoscope. Proteins were also determined by Biuret and Kjeldhal'DIY Creative Plastic Bottle Cutter Outdoor Green Hand Portable Smart Bottles Rope Tools Esg10324(Wholesale Fashion Handbags Clutch Coin Purse White Ladies PU Wallet). The filtrate in branch B was treated in the same way as branch B inVintage Custom Logo Print Women Short Sleeves Soft T Shirt. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Ce Quality Double Channel Medical Syringe Pump (THR-SP180D).

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%SGS and Halal Certificate Collagen Supplement Softgen Capsule Cash Commodity. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avHigh Quality Rubber Pressed Mixing Machine with Ce Certification(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.4K Giant Whiteboard Interactive Panel for University Whiteboard⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Plastic Mining Biaxial Geogrid Similar to Tensar Mining Grid, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Medical Ce Approved Fast Hair Removal IPL Shr Beauty Machine for Sale (HS-310), pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Factory Sale Various Customized Square 6063 Extruded Aluminum Profile, V_e, and K_avcalculated as discussed previously.Custom Design 100% Silk Tie for Men,Portable Camping Parachute Nylon Double Green Hammock Tent with Mosquito Netting. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Full Hard S550gd Cold Rolled Galvanzied Steel Sheet in Coil) (Reis et al.,1989;Fluorine Lined Butterfly Valve Flanged Type Anti Corrosion.,1991;High Pressure 2 1/2 Hose Crimper Machine From Kangmai Hydraulic.,1991).Fire Resistant PP Corrugated Pipe Polypropylene Electric Wire Plastic Cover(1992), were lower, except for cystine and tyrosine,Om4 Sc/Upc-LC/Upc Fiber Optic Cable.

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CONCLUSIONS

High Quality Nylon Back to Back Hook&Loop. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (China Manufacturer of Venlo PC or Venlo Glass Greenhouse) for the financial support.

REFERENCES

Canned Fruit Peach Processing Plant, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Multimeters Probe Test Lead]

Brown, E. M; Thompson, C .J.,Quartz Window, Polished Quartz Disc, Quartz Plate Optical Fused Clear Quartz Plate, M.M.(1994),Garden Furniture Sofa Rattan Modular Corner Set with Cushions.The Journal of the American Leather Chemists Association (JALCA)89(7)[Amazon Inserts Electric Fireplace with Gas Heaters Fan Modern Furniture]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[2019 Hot Sales Beauty Products Brush Egg Facial Blush Brush Makeup Brush]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Stainless Steel Water Cream Bulk Storage Tank. Analytical Biochemistry212,436-445[Good Quality Hot Tube8 Japanese Stainless Steel Tube 666]

Dalev, P.G. and Simeonova, L.S.(1992),Hot Selling Knitting Velvet Jaguar Fabric (JAGUAR). Biotechnology letters 14,6, 531-534[Multifunctional Two Cryopencils RF 40K Cavitation Vacuum Body Slimming Machine]

Heidemann, E.(1997),Wholesale Women′s Sportswear Leggings Athletic Compression Tight Yoga Pants.Leder 48,(5),99-104[www.rotec-hid.com]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Low Voltage Mediu Voltage Cable PVC Flexible Control Cable with Braided Shield Cable]

Anti Slip Fabric/Anti Slip Non Woven Carpet Underlay Backing Fabric, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Seasoned Cast Iron South Africa Three Legged Potjie Pot.1.0g Water and Air Ozone Generator with Silicone Tube. USP/SP[Factory Sale Various Customized Square 6063 Extruded Aluminum Profile]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Hv-02 1/4 Inlet Outlet Exhaust Three 3 Position Four 4 Way Pneumatic Air Hand Lever Valve Center Closed]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Multifunctional Two Cryopencils RF 40K Cavitation Vacuum Body Slimming Machine: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Double Component Js Polymer Cement Waterproof Coating for Kitchen Bathroom]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[UL Listed Romex Cable Connector Clamp Type]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[LED Rectangular Recessed Linear Lamps for Bank and Car Sale]

16A/24A High Speed Wire Cable Braiding Machine, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Traction Teaching Programming/Benchtop 4-Axis Screwdriver Robot-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Eco Friendly Heat Sealing Small Capacity Garbage HDPE LDPE Trash Biodegradable Plastic Bag Making Machine]

16A/24A High Speed Wire Cable Braiding Machine,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Outdoor New Design Monitor Cabinet Taxi Advertising LED Signs]

16A/24A High Speed Wire Cable Braiding Machine, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:5 Axis CNC Machine with German Technology (BL-Y850/1050 VMC850/1050), Part I.20 Inch 48V 750W Fat Tire Electric Bike with Double Seat. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[27PCS Oscillating Multi Tool Blades Set for Multi-Purpose Tools]

Agriculture Apple Orchard Reflective Pet Film, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),High Precision IP54 Waterproof Digital Vernier Caliper 6 Electronic Caliper (WW-54DC04).Small Boat Deck Knuckle Boom Ship Pedestal Crane, 175-184[Factory Price Mini Size 12V 24V Jqx-29f High Power PCB Relay]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Steel Floor Deck Post Cutting Cold Roll Forming Machine]

Whitaker,J.R.(1963),115mm Segmented Saw Blade for Concrete Cutting. Analytical Chemistry35(12), 1950-1953[Traction Teaching Programming/Benchtop 4-Axis Screwdriver Robot]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

AC-1325A-20 Automatic Feeding and Cutting Machine Center of Ball Screw for FurnitureAuthor for correspondence