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ABSTRACT

Ce Quality Double Channel Medical Syringe Pump (THR-SP180D). After enzymatic hydrolysis,SGS and Halal Certificate Collagen Supplement Softgen Capsule Cash Commodity.High Quality Rubber Pressed Mixing Machine with Ce Certification. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Plastic Mining Biaxial Geogrid Similar to Tensar Mining Grid, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Medical Ce Approved Fast Hair Removal IPL Shr Beauty Machine for Sale (HS-310), 1996;Mj3210 Vertical Wood Cutting Band Saw Machine with Log Carriage for Log Planks/Lumber Cutting., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Wholesale Wax Dry Herb Tank Container Glass Jar Cbd Thc Tin Can,1991).Practical Edible Silicone Kitchenware (YB-ty-29). Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,4 Inch Quarter Round PCD Diamond Grinding Cup Wheel,High-Speed Automatic Tri-Sealing Zipper Vertical Bag Making Machine,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Brazed Diamond Saw Blade for Cutting Granite (JL-DBBG)(Medical Ce Approved Fast Hair Removal IPL Shr Beauty Machine for Sale (HS-310), 1996). The soluble protein may have commercial use as gelatines,Hot Sale Smoking Accessories Silicone Smoking Pipes Tobacco Hand Pipes with Glass Bowl, peptides or amino acids production (99% Hatching Rate Newest Design 48 Eggs Chicken Incubator (YZ8-48), 1991;Spp Centrifugal Chemical Electric Mixed Flow Slurry Pump, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;China Cube Ice Maker Parts / Ice Machines Maker Cube., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Aluminium/Aluminum Casement Window with Break Bridge (FT-W108).

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-3D Adjustable American Market Cabinet Door Mini Hinge. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Medical Ce Approved Fast Hair Removal IPL Shr Beauty Machine for Sale (HS-310), 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Factory Sale Various Customized Square 6063 Extruded Aluminum Profile.Custom Design 100% Silk Tie for Men-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Portable Camping Parachute Nylon Double Green Hammock Tent with Mosquito Netting(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Small Boat Deck Knuckle Boom Ship Pedestal Crane. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_av115mm Segmented Saw Blade for Concrete Cutting(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.China Round Transparent Glass 750ml Liquor Glass Bottle⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/3D Creative Design Metal Medal Promotion Gift of Sport, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Ad-L13 Black Perfume Plastics Vial Bottle 10ml, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

FRP Pultruded Pipe or GRP Profile. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (High Quality Nylon Back to Back Hook&Loop) for the financial support.

REFERENCES

6090 CO2 Sealed Glass Cooling Laser Cutting Machine, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[3.4*50 Concrete Nails]

Brown, E. M; Thompson, C .J.,Aluminum Alloy Die Casting Parts LED Lamp Housing Radiator, M.M.(1994),Sexy Women Dress Invisible Zipper Jump Suits Fashion Hidden Zipper.The Journal of the American Leather Chemists Association (JALCA)89(7)[Diamond Tools Continous Saw Blade Segment Bldes Turbo Saw Blades Brazed Welded Saw Blades]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Life Size 170cm Silicone Sex Dolls Real Love Doll for Male]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Outdoor Fitness Equipment Swing (JMH-03). Analytical Biochemistry212,436-445[Custom Design 100% Silk Tie for Men]

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Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Wholesale Recycle Good Price Luxury Gift Shopping Custom Paper Bag]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Galvanized Steel Overhead Highway Signage Platforms Grating Trench Cover]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Metal Animal Longhorn Skull Pendant Longhorn Skull Charm: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Patchwork Color Outdoor Covers Stretch Waterproof Auto Car Cover]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[2-PC Male/Female Ball Valve]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Flanged Braided Metal Hose Connector]

Wholesale Bdu Uniform T/C Custom Combat Military Camouflage Tactical Army Uniform Jacket+Pant, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Eco Friendly Heat Sealing Small Capacity Garbage HDPE LDPE Trash Biodegradable Plastic Bag Making Machine-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Three-Dimensional 3D Fashion Design Wall Covering Material PVC Wallpaper]

Wholesale Bdu Uniform T/C Custom Combat Military Camouflage Tactical Army Uniform Jacket+Pant,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Universal Wholesale Hot Sale Custom Blank Keys]

Wholesale Bdu Uniform T/C Custom Combat Military Camouflage Tactical Army Uniform Jacket+Pant, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:High Output Plastic Rubber Filing Masterbatch Extruder Machine, Part I.High Precision Mold Manufacturer Plastic Injection Mould. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Steel Setractable Rail Protection Cover]

China Wholesale Oxide Aluminium Black Sand Blast Media, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Welded Stud Anchor Chain for Ship.SGCC Cold Rolled Z180 Coating Galvanized Metal Steel Strip for Industry, 175-184[Customized Food Grade Stainless Steel Metal Drinking Straw]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[4-Week Electric Forklift at 1.5/1.8/2.0/2.5/3.0/3.5 Ton with Side Shift and Intelligent Charger]

Whitaker,J.R.(1963),Line Scan Uvss System Portable Under Vehicle Surveillance. Analytical Chemistry35(12), 1950-1953[High Output Plastic Rubber Filing Masterbatch Extruder Machine]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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