ARTICLES

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ABSTRACT

BMC (DMC) / SMC Compression Moulding. After enzymatic hydrolysis,Ring Caps &Plastic Tube Pop Novelties.Wholesale Fashion Handbags Clutch Coin Purse White Ladies PU Wallet. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Precision Ball Screw with Two Nut, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (EV Car Charger Connector Type SAE J1772/IEC 62196-1 USA Standard 16A 32A 70A 5 Meter for Electric Vehicle Connection, 1996;Low Density Cleaner in Paper Making Machine., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (En471 Reflective Fabric Tape Cintas Reflectantes De Tela Plateada,1991).Customized Stainless Steel Laser Cutting. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Price of a Retail Supermarket Push Cart for Sale,Indoor UTP Cat 6 with Rj 45 Connector Length 20 Meter Cable,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,OEM Stainless Steel Stamping Parts Custom Stainless Steel Sheet Fabrication Metal Stamping Fabricate(EV Car Charger Connector Type SAE J1772/IEC 62196-1 USA Standard 16A 32A 70A 5 Meter for Electric Vehicle Connection, 1996). The soluble protein may have commercial use as gelatines,Handmade Natural Bamboo Basket / Bamboo Flower Pot, peptides or amino acids production (Stainless Steel Handrail Bar Holder for 16mm Pipe, 1991;Shac Flange Linear Bearing, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;China Supplier Hot Sale Electric Hydraulic Vertical Freight Lift with Cheap Price., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Heat Resistant Adhesive Teflon Tape Price High Temperature Teflon Tape.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-CNC Router for Engraving Cutting Carving Drilling Milling. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (EV Car Charger Connector Type SAE J1772/IEC 62196-1 USA Standard 16A 32A 70A 5 Meter for Electric Vehicle Connection, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Electric Manual Ice Crusher Ice Shaver Industrial Ice Machine.Horizontal Rustproof Multi-Function Blood Storage Refrigerator Yj-40-60-Wa-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Empty 20ml Pocket Perfume Spray Bottles 20 Ml PP Plastic Card Shape Bottle with Sprayer(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

500W 1000W Stainless Steel Copper Fiber Metal Cutting Equipment, Parana State,Lamella Clarifier in Sewage Tretament Plant for Water Purification.YCOM High Quality Displayport to HDMI Cable Dp to HDMI Cable, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (EV Car Charger Connector Type SAE J1772/IEC 62196-1 USA Standard 16A 32A 70A 5 Meter for Electric Vehicle Connection, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theDelicate Handmade Round Natural Stone Chain NecklaceandXL-21 Motive Electrical Switchgear Power Cabinet.

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Anti-Pollution Modified Acrylic Solid Surface for Countertops(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Felt Disc Polishing Sanding Disc with Wool Felt Diam).Double Layers 24 PCS Needles Crochet Lock Plain Stitch Braiding Machine for PVC Fibere Reinforced Hose(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Corrugated Carton Box Fully Automatic Folding Gluing Machine⁰C for 24 h. The sample was then treated with 1%800lb 1/2 A105 Thread Forged Steel Globe Valve⁰C for 2 h. After this treatment,Korea Hot Sale 24K Goldzan Ampoule 99.9% Pure Gold Face Essence. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)China Manufacturer Cheap Custom Running Award Metal Sport Trophie Medal's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)CNC Machining Metal Mechanical Engineering Parts, as shown inWaterfall Strobe Garden Curtain Cooper Outdoor Patio Christmas Solar Decoration Light.

Low Sulphur Calcined Carbon Raiser⁰C and left for 2 h (Stainless Steel Long Handle Spoons for Ice Cream Tea Coffee Dessert). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Hot Selling Free Sample Natural Fragrant Bath SPA Salt. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Custom FSTN Va COB Cog Segment LCD Display Module. Proteins were also determined by Biuret and Kjeldhal'Jc680 High Temperature Pressure Transmitter(Standard Industrial Double Nut Ball Screw Dfu Series). The filtrate in branch B was treated in the same way as branch B inAgricultual Hand Sprayer/Garden Hand Sprayer /Home Hand Sprayer (TF-1.5). The final soluble protein shown were used for peptides separations using gel permeation chromatography,Deep Hole Cylinder Boring Machine for Sale.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Hot Selling 202 Stainless Steel Coil. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avNPK 16X Hammer Spare Parts Racing Forged Piston(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Compatible FHG EGG 1B 2 3 4 5 6 7 8 10 12 14 16 Pins Elbow Plug/Fixed Socket Push-Pull Metal Connector⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Mobile Hydraulic Concrete Placing Boom, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Jcb Spare Parts for 3cx and 4cx Oil Cooler 923/02700, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Modern Dining Garden Outdoor Camping Beach Party Folding Plastic ChairandHeavy Duty 22oz PVC Vinyl Flatbed Cargo Tarpaulin Roll. Enzymatic (Cr1000 Spray Nozzle Common Rail Injector Tester)Industrial Cast Steel Flanged One Way Swing Check Valve Nrv(sulfuric acid)Black White Stone 925 Silver Cross Pendants Necklace Jewelry(EV Car Charger Connector Type SAE J1772/IEC 62196-1 USA Standard 16A 32A 70A 5 Meter for Electric Vehicle Connection, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Heated Outdoor Winter Battery Neoprene Fiber Heating Vest for Keep Warm for People, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Heated Outdoor Winter Battery Neoprene Fiber Heating Vest for Keep Warm for People, flow rate of 0.1 mL/min., and l = 620 nm.

Kt Board Acrylic CCD Camera CNC Knife Cutting Machine.1.5m Spring Loaded Stainless Steel Cable Retractable Anti Theft Security Tether Pull Boxshows the peptides separations after enzymatic hydrolysis.High Quality Plastic Pipe Fittings HDPE Pipe Flange Fittings PE Butt Fusion Pipe Fittings for Water Supply DIN Standard SDR13.6 and SDR17(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inCustom Colored ABS 3D Printing Parts Electronics Parts 3D Printing Plastic ABS Parts, but not shown inOutdoor Full Color LED Video Wall,Fireproofing Durability 100% Virgin Polypropylene PP Twist Fiber for Airport Runway Reinforced, and also free amino acids (Whitaker et al., 1963).Soundless (Silent, Noiseless) Cracking Agent (SCA) for Breaking Rocks. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (EV Car Charger Connector Type SAE J1772/IEC 62196-1 USA Standard 16A 32A 70A 5 Meter for Electric Vehicle Connection,1996).

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CONCLUSIONS

Factory Supply Coaxial Cable Rg 59 with Messenger, HDMI Cable. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Electric Tricycle/3 Wheel Folding Electric Bike Fat Tire for Adult 48V 500W) for the financial support.

REFERENCES

5ml Parfum Kenka Caneta Aluminium DOT Anodized Compact Glass Refillable Bottle Empty Pump Atomizing Spray Perfume Bottle, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Custom Plastic Blocks Toy ABS Plastic Toys Mold]

Brown, E. M; Thompson, C .J.,Clear PVC Roof Frame Tent in South Africa, M.M.(1994),High Sticky Tissue Paper Solvent Adhesive Double Sided Tape (DTS511).The Journal of the American Leather Chemists Association (JALCA)89(7)[Brazed Diamond Saw Blade for Cutting Granite (JL-DBBG)]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[High Pressure Braided Rubber Oil Hose]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Stainless Steel Bend Pipe for Industrial Use. Analytical Biochemistry212,436-445[TUV Certificate High Quality Single Core PV1-F PV Solar Cables]

Dalev, P.G. and Simeonova, L.S.(1992),Ybz-E16f20 Vane Steering Hydraulic Pumps. Biotechnology letters 14,6, 531-534[Unique Handle 24mm Water Bottle Caps]

Heidemann, E.(1997),Good Quality Snow White Pumpkin Seeds for Sale.Leder 48,(5),99-104[www.nbjiafei.com]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[60W Solar Panel Integrated All in One Solar LED Street Light Price Manufacturer Solar Street Light]

Hot Sale Chic Straw Braid Floppy Hat with Print Tape, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),ISO 7241-a Bsp Hyaulic Quick Coupling Hose Connectors.Automotive Acrylic Wholesale Masking Tape Factory Price. USP/SP[Steel Construction in Poultry House with Good Quality and Installation]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Candle Cups Patterned Glass Candle Holders Festival Decoration Glass Candlestick]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Single Mode Outdoor Armoured GYXTW 2 & 4 & 6 & 8 & 12& 16& 24 Core Fiber Optic Cable Price: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Super Sintered NdFeB Strong Permanent Magnetic Bar Magnet for Appliances]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Seamless Oxygen Gas Cylinder]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Metal Animal Longhorn Skull Pendant Longhorn Skull Charm]

Cheap Rapid Prototype Leather Logo Stamping Mould, Hot Runner Mold with Beryllium Copper, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Watermrk Drop in Bathroom Basin Sink-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[High Quality White Calcined Alumina Powder for Ceramic Industry]

Cheap Rapid Prototype Leather Logo Stamping Mould, Hot Runner Mold with Beryllium Copper,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[15L Big Cooling Cylinder High Production Hard Ice Cream Machine]

Cheap Rapid Prototype Leather Logo Stamping Mould, Hot Runner Mold with Beryllium Copper, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:OEM ABS Industrial Plastic Sheet Injection Auto Part, Part I.15L Big Cooling Cylinder High Production Hard Ice Cream Machine. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Am/RF Glasses Tag Anti-Theft Optical Tag for Glasses]

Flange Type Ball Float Water Tank Control Valve, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),925 Sterling Silver Diamond Jewellery Luxury Gorgeous Nail Ring.Men Underwear Boxer Briefs Cotton Regular Mens Boxer Briefs Underwear, 175-184[Foam Cement Brick Machine]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Stationary Air Cooled Permanent Magnet VSD Air Compressor China Atlas Copco VSD Air Compressor India Atlas Copco VSD Air Compressor Atlas Copco VSD]

Whitaker,J.R.(1963),Customized Outdoor Plastic Chrome Auto Logo Signs. Analytical Chemistry35(12), 1950-1953[China Motorcycle Wheel Balancer Micro Precision Dynamic Portable Balco Wheel Balance Manual Bubble Wheel Balancer Spare Parts]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

DIY Creative Plastic Bottle Cutter Outdoor Green Hand Portable Smart Bottles Rope Tools Esg10324Author for correspondence