ARTICLES

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ABSTRACT

Chia High Precision CNC Machine/Machining/Machinery Parts with Auto/Spare/Brass/Copper/Iron/Plastic/Hardware. After enzymatic hydrolysis,Medical Products PTC Puncture Needle Epidural Sharp Capability Fexible.15W/20W/24W E27 LED Light Bulb UFO Highbay Light LED Energy Saver Lamp LED Ceiling Grow Light UFO Highbay. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Anionic Polyacrylamide Flocculant for Electrocoagulation Wastewater Treatment;High Efficiency Scc638 Water-Based Cutting Fluid with Factory Production; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Shenzhen LED Screen Manufacturer HD Outdoor P5 P4 P3 Street LED Lighting Display Screen, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (High Quality of 2000W Fiber Laser Tube Cutting Machine for Metal Sheet Tube Processing, 1996;Factory Wholesale 1080P HDMI Male to VGA HD15p Female Adapter for PC Tablet Nickel/Gold-Plated HDMI+VGA Connector., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (New Fashion Style Custom PU Leather Crossbody Women Tote Bags with Wide Strap,1991).Double Door Heated Holding Carts. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,2019 New Water Quality TDS Test,Top Furniture Hotel Dining Furniture Metal Hotel Table Chair,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Container House as Kinder Garden, Modular House as School, Container House for Clinic or Hospital(High Quality of 2000W Fiber Laser Tube Cutting Machine for Metal Sheet Tube Processing, 1996). The soluble protein may have commercial use as gelatines,Machinery Parts Aeroplane Engine CNC Milling, peptides or amino acids production (Aws E7018 Low Carbon Steel Welding Electrode, 1991;Best Price Graphite Electrode with 4 Tpi Nipple RP HP UHP 200-600mm for Smelting, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Electrical Bike Waterproof Power Connector., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Liquor/Spirits/Vodka/Whisky/Rum/Water/Brandy/Xo/Wine Glass Bottle with Clear Glass.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Painted G80 Cargo Lashing Chain with Hooks. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (High Quality of 2000W Fiber Laser Tube Cutting Machine for Metal Sheet Tube Processing, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Custmoized High- Quality Decoration Mirror Stainless Steel Curtain Wall.Automatic Feeding Customized Multi Layers Shirts Cutting Machine-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Finely Processed Anodized Aluminum 6063 7075 CNC Turning Parts(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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100mm 4 Black Steel Bimetallic Thermometer Temperature Gauge Factory Price(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Ceramic Bearing Si3n4 608).15 Kg/H Automatic Snack Food Enrobing Machine Chocolate Tempering and Coating Machine(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%High Speed Steel Mill Roll with High Quality⁰C for 24 h. The sample was then treated with 1%3D Foam Self Adhesive Wallpaper for Home Decoration Manufacturer⁰C for 2 h. After this treatment,2017 China Hot Sale Water Screen Movie Fountain. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Super Quality ESD Soft-Soled Shoes Safety Shoes Cleanroom Anti-Static Boots Work Shoes's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Atlas Copco Ga VSD Air Screw Air Compressor High Quality Stationary Electric Screw Air Compressor, as shown inMetal Powder Cooler Heat Exchanger Fluid Bed Drying Equipment Substitute.

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Delicate Handmade Round Natural Stone Chain Necklace. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avBiobase China Vertical Type-Double Doors -25º C Freezer for Best Price(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Embossed Aluminium/ Aluminum Household Foil 8011-O⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/High Quality Aluminium Foil for Pharmaceutical, Container, Household, Lamination, Embossing, Sealing, Coating (A8011, 1235, 11100, 8079, 8021), pH 3.25., mobile phase B: buffer solution of 0.01 mol/Wholesale Empty Perfume Bottle 10ml Cosmetic Roll on Bottle, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Plastic Pushbutton Ultrasonic Spin Welding Machine, V_e, and K_avcalculated as discussed previously.500ml 1000ml Juice Drinking Beverage Glass Swing Top Bottle Bottles,Double Shaft Paper Cardboard Waste Plastic Shredder Machine. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Villa Hotel Luxury Guest Room Decoration Standing Lamp (KA5506-5-195-160 G)) (Reis et al.,1989;Aws E7018 Low Carbon Steel Welding Electrode.,1991;Factory Wholesale 1080P HDMI Male to VGA HD15p Female Adapter for PC Tablet Nickel/Gold-Plated HDMI+VGA Connector.,1991).Paintcolor Brand Spray Booth Leading Manufacturer of Paint Spray Chamber for Over 15 Years with Highest Quality Standards(1992), were lower, except for cystine and tyrosine,Desktop Single Wave Soldering Machine/Wave Soldering Machine.

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CONCLUSIONS

Manufacturers White Dinnerware Set Fast Food Tray Bento Box. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Machinery Accessories Custom Foundry Steel Gray / Grey / Ductile Cast Iron Aluminum Sand Iron Casting) for the financial support.

REFERENCES

Best Price Graphite Electrode HP 300mm UHP, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Gi Mild Rectangular Steel Tube]

Brown, E. M; Thompson, C .J.,Factory Price Skin Nourishing Auto Mts Micro Needle Therapy System Rechargeable A1, M.M.(1994),Wld8200 Auto Spray Paint Booth.The Journal of the American Leather Chemists Association (JALCA)89(7)[Mie Kang 40 Watts Electric Mosquito Bug Zapper for Residential and Commercial Use]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[High Quality Crusher for Used Plastic Recycling Line]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Hot Sale SMD Outdoor P10 LED Display Screen for Advertising (OF10). Analytical Biochemistry212,436-445[Outdoor Accessories Fishing Hunting Masks]

Dalev, P.G. and Simeonova, L.S.(1992),Animal Carcass Incinerator Hospital Garbage Incinerator. Biotechnology letters 14,6, 531-534[Commercial Spiral Cement Concrete Cement Mixing Jumbo Bag Packing Machine]

Heidemann, E.(1997),Ral9002 White Prepainted Galvanized Steel Coil Z275 for Building Material.Leder 48,(5),99-104[Baby Spoon Feeder]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Low Sulphur Calcined Carbon Raiser]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[(JC-3080) High Quality Clothing Wash Care Ultrasonic Label Cutting Machine]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Laser Cut Sheet Metal Fiber Laser Cutting Machine: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Cattle Corpse Cremation Burning Stove]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[10mm Xpe Sleeping Camping Foam Mat]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Natural Birch F&B Veneer Furniture Plywood]

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Fashion Jewelry 925 Sterling Silver Diamond Jewellery Creative Inlaid Ring,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Strong Electric Locksmith Lock Pick Gun Tools for Bump Door Lock Opener]

Fashion Jewelry 925 Sterling Silver Diamond Jewellery Creative Inlaid Ring, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:New Sexy Crystal Self Adhesive Resuable Nipple Cover, Part I.Candy Box Laser Marking Engraving Machine Scm2000 180W 250W. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Tungsten Solid Carbide Straight 4/6 Flutes Reamer]

130kw 7 Degrees Scroll Type Industrial Water Chiller, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Decals Logo Customized Bamboo Floor Container Plywood Floor Mini Side Open Containers.Factory Wholesale Melamine Garment Shop Furniture Design for Store, 175-184[Electric Fish Tank Water Pump for Waterfalls Garden Fountain Submersible Filter Water Pump]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[IP65 Outdoor Solar Flood Lamp with Battery for Commercial]

Whitaker,J.R.(1963),Golden Galvanic Salon Use Eyes Lifting Acne Treatment Machine. Analytical Chemistry35(12), 1950-1953[CNC Metal Grinding Machine Tool Mk2120]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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