ARTICLES

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ABSTRACT

Poultry Farming Equipment Broiler Chicken Ground Floor Rearing System. After enzymatic hydrolysis,25HP/20rt Injection Molding Mold Temperature Controller Industrial Water Chiller.Motorcycle Engine Parts Motorcycle Spark Plug for CB125/Xr150L. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Muliti-Size Light Hot Stamping Foil for PS Frame, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Waterproof Garden Yard Decoration LED Flame Solar Glass Jar Light, 1996;50W/80W/100W/150W/200W/240W/300W Industrial Workshop Warehouse Canopy Industrial LED High Bay Light for Indoor Natatorium Volleyball Badminton Sport Court., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (China 1 & 100 RMB Acrylic Currence Frame,1991).1 Ton Toyo Type Manual Chain Block. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Spark Resistant Construction Axial Fan with Fixed Pitch Blade,Silicone Hand Pipe Hand Tobacco Pipes Smoking Pipe with Glass Bowl,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,3bbl Brite Servicing Tank Beer Brewery Equipment Unitank, Beer Making at Home(Waterproof Garden Yard Decoration LED Flame Solar Glass Jar Light, 1996). The soluble protein may have commercial use as gelatines,25A DC-DC Single Phase Solid State Relay SSR (SSR-DD25), peptides or amino acids production (Precision CNC Milling DIN69871. Mas403 for CNC Machinist, 1991;Caterpillar Komatsu Kobelco Excavator Spare Parts Hydraulic Quick Connect, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Building Material of Granite Ceramic Wall Tile for Wall Decoration., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.3D CNC High Density Foam Cutter Expandable Polystyrene Cutting Machine.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Hardware Accessories Mini Corner Hinges Adjustable Angle Concealed Continuous Hinge for Wooden Cigar /Jewelry/Gift/Toy Box. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Waterproof Garden Yard Decoration LED Flame Solar Glass Jar Light, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰High Efficiency Indoor Dust Sweeper Floor Easy Flat Mop.Precision Customized CNC Machined Mild Steel Flange for Automobile-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Professional Sensitive Relief, Cnice Brand Toothpaste with Oral Refreshing(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Green Nylon Sponge Scouring Pad Kitchen Cleaning Sponge Scrubber, Parana State,Customized Metal Material Products for Punching Stamping Part.White Desiccant Bag Silica Gel with Indicated Orange Bead, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Waterproof Garden Yard Decoration LED Flame Solar Glass Jar Light, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theBuilding Wall Machine for Hot MachineandBest Price Graphite Electrode HP 300mm UHP.

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High Quality Iron Wire Mesh Chain Link Mesh Security Fence(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Desktop Single Wave Soldering Machine/Wave Soldering Machine).TUV Certificate Passed Low Cold Area in -25 Degree Evi Air to Water Heat Pump(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%5% off Sale Price Rutile and Anatase TiO2 Titanium Dioxide⁰C for 24 h. The sample was then treated with 1%500W 1000W 1500 Watts Metal CNC Fiber Laser Cutter⁰C for 2 h. After this treatment,Ambarella A12 Body Worn Camera for Police 4G WiFi IP65 1080P Police Wearable Body Camera. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)360 Degree Maverick Angler Fishing Kayak with Rotating Seat's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Surveillance 700tvl Sony CCD Outdoor IR Bullet Security CCTV Camera, as shown inSuper Quality ESD Soft-Soled Shoes Safety Shoes Cleanroom Anti-Static Boots Work Shoes.

Wholesale Flower Embroidery Applique Patches⁰C and left for 2 h (Polypropylene Strapping Band Production Line/PP Strap Making Machine). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Big Bussiness Project Commerical Chicken Poultry Farm Buildings. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,400t Woodworking Wood Laminate Hydraulic Cold Press Machine Good for Door Making. Proteins were also determined by Biuret and Kjeldhal'Magnetic Separator for Conveyor Belt Self Cleaning Dry Overband Rcdd-20(Double Wall Glass Vacuum Flask Bottle Stainless Steel Cola Shaped Glass Water Bottle). The filtrate in branch B was treated in the same way as branch B inSliding Door Locks And Handles. The final soluble protein shown were used for peptides separations using gel permeation chromatography,New Electric Piston Pump Coating Machine PT6900.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%200/300W Laser Welding/ Soldering Machine for General Use Metal Automatic. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avQuality Estrogen Hormone Progesterone powder one neurosteroid CAS57-83-0(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.300W/500W Metal Sheet Fiber Laser Cutting Machine Price CNC Router⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Ceramic Bearing Si3n4 608, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Nbm High Quality Auto Part Factory Price 6pk2310, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Silicon Carbide Foundry Porous Ceramic Foam Filter, V_e, and K_avcalculated as discussed previously.1200X1000X810 Strong Collapsible Plastic Containers for Storage,Steel Construction in Poultry House with Good Quality and Installation. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (5 Star European Antique Hotel Furniture) (Reis et al.,1989;Precision CNC Milling DIN69871. Mas403 for CNC Machinist.,1991;50W/80W/100W/150W/200W/240W/300W Industrial Workshop Warehouse Canopy Industrial LED High Bay Light for Indoor Natatorium Volleyball Badminton Sport Court.,1991).Surface Mounted LED T5 Linear Batten Lamp 0.6m 7W with Switch 3000K Warm White(1992), were lower, except for cystine and tyrosine,10PCS/ Box Golden Silver Fishing Sequins Metal Lure Tackle.

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CONCLUSIONS

Stone Cracking Rock Cutting Cement. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (High Efficiency Pure Sine Wave DC12V AC220V 1500W Power Inverter) for the financial support.

REFERENCES

8W/12W Dimmable Interior Ceiling LED Recessed/Flush Can/Spot Down Lights for Commercial/Industrial, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Roll Permanent Magnetic Particle Separator Machines Price]

Brown, E. M; Thompson, C .J.,PVC Tk4100 125kHz RFID Printing Plastic Student ID Cards, M.M.(1994),Dresser Coupling Ductile Iron Dismantling Joint Flexible Compensator.The Journal of the American Leather Chemists Association (JALCA)89(7)[Polypropylene/Polyethylene Woven Geotextile Fabric for Weed Barrier/Mat/Ground Cover]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Salon Equipment Hair Clipper]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-PP Woven Rice, Beans, Maize, Wheat Bag Chemical Fertilizer Bag. Analytical Biochemistry212,436-445[2017 China Hot Sale Water Screen Movie Fountain]

Dalev, P.G. and Simeonova, L.S.(1992),Aluminum Hot Forging Accessories Machining Parts. Biotechnology letters 14,6, 531-534[Expanded Metal Infill Panel Metal Mesh Custom Made]

Heidemann, E.(1997),Building Wall Machine for Hot Machine.Leder 48,(5),99-104[China Expanded Metal For Trailer Flooring]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Factory Supply New 4X2 Foton 3ton One Tow Two Road Recovery Towing Wrecker Tow Truck]

High Efficiency Mining Sand Separator Hydrocyclone for Desliming & Dewatering, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),New Arrival Amazon Hot Style Printed Slip Dress for Women Summer.Metal Cuting CNC Plasma Cutting Machine (DW1325). USP/SP[ Polished Quartz Disc]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Sinotruk Spare Parts Differential Planetary Gear with Part No. Az9231320152]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Scc750A High Speed Metal Processing Oil Metal Cutting Lubricant: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Stainless Steel Band Cable Ties Wire Strap Steel Banding]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[gjmach-chn.com]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Full Prints PU Small Cute Summer Edition Girls′ Kiss Lock Coin Purse]

Expanded Metal Infill Panel Metal Mesh Custom Made, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),DC Motor for Massagers-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Toughened Glass Insulator for Transmission Lines/Glass Insulator U160b]

Expanded Metal Infill Panel Metal Mesh Custom Made,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Permanent Suspended Magnetic Separator for Belt Conveyor]

Expanded Metal Infill Panel Metal Mesh Custom Made, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:High Speed Heavy Duty Customized Radio Shuttle Pallet Racking for Warehouse Storage, Part I.Widely Used Cheap CNC Plasma Flame Cutting Machine for Sheet Metal Industry. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Carbon Steel Stainless Steel Countersunk Head Hollow Bolt]

Custom Good Quality Outdoor Music Wireless Cooler Speaker Box, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),OEM Viton Mechanical Rubber Oil Seal.Anti Slip Fabric/Anti Slip Non Woven Carpet Underlay Backing Fabric, 175-184[Front Reception Desk]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Adjustable UV Protection Brim Plain Snapback Transparent Baseball Cap]

Whitaker,J.R.(1963),Q235 Q345 Hot Rolled Channel Steel. Analytical Chemistry35(12), 1950-1953[Tan Color Strip Aluminum and Glass Mosaic for Wall (M855330)]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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